##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921325_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2102108
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.032241920966953	31.0	30.0	34.0	16.0	34.0
2	29.814217442681347	33.0	31.0	34.0	25.0	34.0
3	30.077675837778077	33.0	31.0	34.0	26.0	34.0
4	32.24484707731477	37.0	35.0	37.0	19.0	37.0
5	31.857784186159797	37.0	33.0	37.0	19.0	37.0
6	32.01405208485958	37.0	35.0	37.0	17.0	37.0
7	32.227088712853956	37.0	35.0	37.0	17.0	37.0
8	32.48185059949346	37.0	35.0	37.0	17.0	37.0
9	34.211444416747376	39.0	37.0	39.0	17.0	39.0
10-11	34.40701120018572	39.0	37.0	39.0	19.0	39.0
12-13	34.41741813455826	39.0	37.0	39.0	20.5	39.0
14-15	35.82964410011284	40.5	38.0	41.0	23.5	41.0
16-17	35.840913026352595	41.0	38.0	41.0	24.0	41.0
18-19	35.85965754376083	41.0	38.0	41.0	23.5	41.0
20-21	35.84542896939644	41.0	38.0	41.0	23.0	41.0
22-23	35.79358101486698	41.0	38.0	41.0	23.0	41.0
24-25	35.73983068424648	41.0	38.0	41.0	22.0	41.0
26-27	35.654615271908014	40.0	37.5	41.0	20.5	41.0
28-29	35.58179479836431	40.0	37.0	41.0	19.5	41.0
30-31	35.48806483777237	40.0	37.0	41.0	18.0	41.0
32-33	35.370510221168466	40.0	37.0	41.0	17.0	41.0
34-35	35.28250903378894	40.0	37.0	41.0	16.0	41.0
36-37	35.20245011198283	40.0	36.0	41.0	15.5	41.0
38-39	35.10313147564254	40.0	36.0	41.0	15.0	41.0
40-41	34.97967421274264	40.0	36.0	41.0	9.0	41.0
42-43	34.84517137083347	40.0	35.0	41.0	8.5	41.0
44-45	34.7080521076938	40.0	35.0	41.0	7.0	41.0
46-47	34.51549278153168	40.0	35.0	41.0	2.0	41.0
48-49	34.31860541894137	40.0	35.0	41.0	2.0	41.0
50-51	34.140596962667956	40.0	35.0	41.0	2.0	41.0
52-53	33.95985172978743	39.0	35.0	41.0	2.0	41.0
54-55	33.753257444431966	39.0	35.0	41.0	2.0	41.0
56-57	33.51092688862799	39.0	34.5	41.0	2.0	41.0
58-59	33.4502970351666	38.5	35.0	41.0	2.0	41.0
60-61	33.21001727789438	38.0	34.0	41.0	2.0	41.0
62-63	32.924477952607575	37.0	34.0	40.0	2.0	41.0
64-65	32.62344917577974	37.0	34.0	40.0	2.0	41.0
66-67	32.307354569793745	36.0	34.0	39.0	2.0	41.0
68-69	31.97842047126028	35.5	33.5	39.0	2.0	41.0
70-71	31.651371147438667	35.0	33.0	38.5	2.0	40.5
72-73	31.3133868478689	35.0	33.0	37.0	2.0	39.5
74-75	30.983703025724655	35.0	33.0	37.0	2.0	39.0
76-77	30.689386796491902	35.0	33.0	36.0	2.0	39.0
78-79	30.43498002957032	35.0	33.0	36.0	2.0	37.5
80-81	30.207023378437263	35.0	32.0	35.5	2.0	37.0
82-83	30.005922150526995	35.0	32.0	35.0	2.0	37.0
84-85	29.831105252441834	35.0	32.0	35.0	2.0	36.0
86-87	29.691026816890474	35.0	32.0	35.0	2.0	36.0
88-89	29.5688118783621	35.0	32.0	35.0	2.0	36.0
90-91	29.46910934167036	35.0	32.0	35.0	2.0	35.5
92-93	29.400552683306472	35.0	32.0	35.0	2.0	35.0
94-95	29.33136927312964	35.0	32.0	35.0	2.0	35.0
96-97	29.26961340711324	35.0	31.5	35.0	2.0	35.0
98-99	29.211697020324358	35.0	31.0	35.0	2.0	35.0
100-101	29.156892985517395	35.0	31.0	35.0	2.0	35.0
102-103	28.480634677190707	34.0	29.5	35.0	2.0	35.0
104-105	29.029814357778	35.0	31.0	35.0	2.0	35.0
106-107	29.088058748646596	35.0	31.0	35.0	2.0	35.0
108-109	29.1607491146982	35.0	31.5	35.0	2.0	35.0
110-111	29.269261141673027	35.0	32.0	35.0	2.0	35.0
112-113	29.231282836086443	35.0	32.0	35.0	2.0	35.0
114-115	29.166984046490477	35.0	32.0	35.0	2.0	35.0
116-117	29.102935006193782	35.0	32.0	35.0	2.0	35.0
118-119	29.023210034879273	35.0	32.0	35.0	2.0	35.0
120-121	28.925907232168854	35.0	32.0	35.0	2.0	35.0
122-123	28.810459072511975	35.0	32.0	35.0	2.0	35.0
124-125	28.2792775157128	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	151102.0
3	9569.0
4	6997.0
5	6443.0
6	6621.0
7	7129.0
8	7214.0
9	7437.0
10	7500.0
11	7407.0
12	7997.0
13	9206.0
14	10274.0
15	8696.0
16	6289.0
17	5559.0
18	5053.0
19	4638.0
20	4484.0
21	4585.0
22	5267.0
23	6620.0
24	8684.0
25	11156.0
26	13615.0
27	15818.0
28	18899.0
29	22472.0
30	27060.0
31	33518.0
32	42829.0
33	57797.0
34	87613.0
35	149450.0
36	343224.0
37	719955.0
38	253599.0
39	332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98057423723376	17.665261244333436	11.434046356022503	27.9201181624103
2	15.451204219764161	19.95939314250267	37.62727700003996	26.962125637693212
3	18.30353150266304	24.23453029054644	28.59286963372006	28.86906857307046
4	13.56270266283803	16.149602346666157	31.87045755707866	38.41723743341716
5	14.133274109292184	37.4657511722881	32.22351801289362	16.17745670552609
6	33.29563757239299	34.77053181484147	16.89426910204128	15.039561510724253
7	29.64935379479315	31.406653194244132	20.16780731802832	18.7761856929344
8	27.73536145117122	35.168722251507674	18.426378599335084	18.669537697986026
9	28.060197263881832	15.682963867940147	17.938815084616426	38.31802378356159
10-11	26.66783694919713	25.3121169673603	26.03674068703672	21.983305396405854
12-13	26.461727903797605	22.805040116064323	26.418817804145263	24.31441417599281
14-15	24.088771842360146	23.897392522172982	23.905931569643425	28.107904065823448
16-17	24.18267757888748	26.759947633518355	24.409973226875117	24.647401560719047
18-19	24.40609616632447	25.9928129287363	25.744514553962023	23.85657635097721
20-21	25.231196494185838	25.133794267468655	25.51093949502119	24.12406974332432
22-23	24.97150479423512	24.70677053700381	25.39819552563427	24.923529143126803
24-25	24.584512308596896	25.072451082437247	25.40613993191596	24.936896677049894
26-27	24.23850249368729	25.184386339807467	25.613408064666515	24.963703101838725
28-29	24.426361538037057	25.349649018984756	25.51605340924443	24.70793603373376
30-31	24.78535831650895	25.306002355730534	25.371650742968487	24.536988584792027
32-33	24.499954331556705	25.46346334251142	25.39859988164262	24.637982444289257
34-35	24.626708047350565	25.477330375033063	25.563006277508098	24.33295530010827
36-37	24.766139513288564	25.248512445602223	25.56507562884495	24.42027241226426
38-39	24.544052922114375	25.568667261625	25.434468638147994	24.452811178112636
40-41	24.568671067328605	25.536223638366824	25.393795180837518	24.501310113467053
42-43	24.414730356385114	25.47968515414051	25.264282329927862	24.84130215954651
44-45	24.14892574501405	25.567501764895052	25.575041815168394	24.708530674922507
46-47	24.246018758313085	25.49759574674565	25.260143627254166	24.996241867687104
48-49	24.311952573321637	25.637574282577297	25.158935696928985	24.891537447172077
50-51	24.25096617300348	25.754409383342814	25.291873681085843	24.70275076256786
52-53	24.388994285736032	25.552921162946905	25.259691699950714	24.798392851366344
54-55	24.056185505216668	25.441865974536036	25.571307468503047	24.93064105174425
56-57	24.16738340751284	25.61069650084582	25.175799721041926	25.046120370599418
58-59	24.218570121040404	25.487796059955052	25.395127177100317	24.898506641904223
60-61	24.510610301659096	25.488961556685002	25.48325300127301	24.51717514038289
62-63	24.3645188544071	25.511962277865834	25.40580693285026	24.7177119348768
64-65	24.217713837728606	25.264686685936212	25.43732291585399	25.08027656048119
66-67	24.157774005902645	25.491411478382652	25.322271738654724	25.028542777059982
68-69	24.053973439994518	25.445719249439136	25.45763585886168	25.042671451704667
70-71	24.034088638642732	25.2806230697947	25.55318280506996	25.132105486492605
72-73	24.033351283568685	25.626989669417554	25.175966220574775	25.163692826438982
74-75	23.91558854254872	25.705601234570253	25.257527206023667	25.121283016857365
76-77	23.96960574813473	25.254839427850516	25.304194646516738	25.471360177498013
78-79	23.845849023932168	25.393747609542423	25.50387515769884	25.256528208826566
80-81	24.104946082694134	25.58013194374409	25.124755721399662	25.190166252162115
82-83	24.13981584200241	25.481516649001858	25.28552291319	25.093144595805732
84-85	23.98509020468977	25.622232539907557	25.04034045824477	25.3523367971579
86-87	23.703230281222467	25.73340665655618	25.293062963463342	25.270300098758007
88-89	23.68824532326598	25.458230500050426	25.2839054891566	25.569618687526997
90-91	23.89381688683888	25.353615864482475	25.369718774685087	25.382848473993562
92-93	23.933089703715048	25.40492879018961	25.333642865365935	25.328338640729402
94-95	23.910105432311486	25.364003830442506	25.259204217482743	25.466686519763265
96-97	23.66339549183935	25.227237156765693	25.422446801982208	25.686920549412758
98-99	23.49749560797809	25.352967284729083	25.301590261580404	25.84794684571242
100-101	23.589725171113948	25.38347220980083	25.434135639082296	25.59266698000293
102-103	23.40257969619068	25.25431614360442	25.457849929689623	25.885254230515276
104-105	23.40448254799468	25.40973156469601	25.295275028685495	25.890510858623824
106-107	23.475748481700847	25.23158328637645	25.38000590360474	25.912662328317964
108-109	23.37807276349628	25.2295563765343	25.45816132264052	25.934209537328893
110-111	23.262623867233685	25.66163019400366	25.364665183546258	25.711080755216393
112-113	23.276597789249948	25.6015911767896	25.364137249939585	25.757673784020866
114-115	23.194636612975238	25.70856967386731	25.319554760479317	25.77723895267814
116-117	22.96438147761673	25.58921589871435	25.460487147461734	25.985915476207182
118-119	23.061775249940062	25.341396048910998	25.455638731480462	26.14118996966848
120-121	22.979983640212158	25.42117890848009	25.573668876276667	26.02516857503108
122-123	22.885945614356825	25.577659846994443	25.350432583338268	26.185961955310468
124-125	22.69540101797887	25.545256627102386	25.22940678790537	26.529935567013375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	81.0
1	70.5
2	83.5
3	109.0
4	100.0
5	127.5
6	236.5
7	476.0
8	664.5
9	700.5
10	637.0
11	564.0
12	582.5
13	637.0
14	704.0
15	800.5
16	858.5
17	905.0
18	970.0
19	1025.5
20	1140.0
21	1213.0
22	1320.5
23	1551.0
24	1829.0
25	2156.0
26	2650.5
27	3395.0
28	4324.0
29	5628.0
30	7548.0
31	9982.5
32	13089.5
33	16422.0
34	19862.0
35	23623.0
36	28182.0
37	32940.0
38	37046.0
39	41718.0
40	46426.5
41	50501.5
42	54758.5
43	60214.5
44	66090.0
45	71415.5
46	77033.0
47	82397.5
48	87797.0
49	92703.0
50	96213.5
51	98400.5
52	97759.0
53	96311.0
54	95109.5
55	91905.0
56	86236.5
57	78770.0
58	72219.0
59	65177.0
60	56171.0
61	47621.5
62	39282.0
63	30312.5
64	22348.0
65	16909.5
66	12847.5
67	9816.0
68	7569.0
69	5748.0
70	4495.5
71	3450.5
72	2646.0
73	2086.0
74	1578.5
75	1193.5
76	884.0
77	599.5
78	441.0
79	344.0
80	256.0
81	216.5
82	212.5
83	192.0
84	162.5
85	152.5
86	146.5
87	123.5
88	123.5
89	129.0
90	114.0
91	104.5
92	99.5
93	90.5
94	77.0
95	64.0
96	57.0
97	50.5
98	30.5
99	10.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.350315017116152
2	0.0
3	0.0
4	0.019171231925286428
5	0.18367277038096996
6	0.051186713527563765
7	0.025260357698082114
8	0.009133688659193534
9	0.00723083685519488
10-11	0.0024499216976482654
12-13	0.05727583930035944
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.6649953284988213E-4
92-93	4.757129509996632E-4
94-95	4.7571295099966316E-5
96-97	4.281416558996969E-4
98-99	4.7571295099966316E-5
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.189282377499158E-4
108-109	4.757129509996632E-4
110-111	4.043560083497137E-4
112-113	7.611407215994611E-4
114-115	1.6649953284988213E-4
116-117	6.422124838495453E-4
118-119	1.9028518039986526E-4
120-121	1.189282377499158E-4
122-123	0.0013082106152490739
124-125	7.135694264994948E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2102108.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.50109416833193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.50419348512985	52.391065597815064
2	9.346808967021003	11.8706519277642
3	3.120464484093838	5.944587271601344
4	1.4516895157293594	3.6873549056604094
5	0.8691249880958171	2.759519385656142
6	0.5716183558310273	2.1779034625183877
7	0.40150312451096265	1.7847121402915098
8	0.30037833566777633	1.5259482383493026
9	0.22340689021190965	1.27679237758806
>10	1.1456461235299653	12.823711733437662
>50	0.05136063003245197	2.1861570687471708
>100	0.013430403532240428	1.2740801539715276
>500	1.4987864554569447E-4	0.08413432858403361
>1k	2.248179683185417E-4	0.21338140801516217
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.7571295099966316E-5
2	0.0	0.0	0.0	0.0	4.7571295099966316E-5
3	0.0	0.0	0.0	0.0	4.7571295099966316E-5
4	0.0	0.0	0.0	0.0	4.7571295099966316E-5
5	0.0	0.0	0.0	0.0	4.7571295099966316E-5
6	0.0	0.0	0.0	0.0	4.7571295099966316E-5
7	0.0	0.0	0.0	0.0	4.7571295099966316E-5
8	0.0	0.0	0.0	0.0	4.7571295099966316E-5
9	0.0	0.0	0.0	4.7571295099966316E-5	4.7571295099966316E-5
10-11	0.0	0.0	0.0	4.7571295099966316E-5	4.7571295099966316E-5
12-13	0.0	0.0	0.0	4.7571295099966316E-5	1.9028518039986526E-4
14-15	0.0	0.0	0.0	9.514259019993263E-5	1.9028518039986526E-4
16-17	0.0	0.0	0.0	9.514259019993263E-5	1.9028518039986526E-4
18-19	0.0	0.0	0.0	9.514259019993263E-5	1.9028518039986526E-4
20-21	0.0	0.0	0.0	2.1407082794984843E-4	2.616421230498148E-4
22-23	0.0	0.0	0.0	2.8542777059979794E-4	3.805703607997306E-4
24-25	0.0	0.0	0.0	5.232842460996296E-4	4.757129509996632E-4
26-27	0.0	0.0	0.0	8.80068959349377E-4	4.994985985496464E-4
28-29	0.0	0.0	0.0	0.0036154184275974405	5.232842460996296E-4
30-31	0.0	0.0	0.0	0.012368536725991242	5.232842460996296E-4
32-33	0.0	0.0	0.0	0.02958934555217905	6.659981313995285E-4
34-35	0.0	0.0	0.0	0.050829928814314015	7.135694264994948E-4
36-37	0.0	0.0	0.0	0.08286919606414132	7.135694264994948E-4
38-39	0.0	0.0	0.0	0.14211924411114937	7.135694264994948E-4
40-41	0.0	0.0	0.0	0.21416597054004838	7.135694264994948E-4
42-43	0.0	0.0	0.0	0.2785299328103028	7.37355074049478E-4
44-45	0.0	0.0	0.0	0.3461763144424549	8.087120166994274E-4
46-47	0.0	0.0	0.0	0.4150833353947561	8.324976642494105E-4
48-49	0.0	0.0	0.0	0.49626375048284865	9.0385460689936E-4
50-51	0.0	0.0	0.0	0.583485720048637	9.0385460689936E-4
52-53	0.0	0.0	0.0	0.6735857529679731	0.001046568492199259
54-55	0.0	0.0	0.0	0.7701792676684547	0.0011417110823991918
56-57	0.0	0.0	0.0	0.868746991115585	0.0012844249676990907
58-59	0.0	0.0	0.0	0.9825851002898044	0.001331996262799057
60-61	0.0	0.0	0.0	1.100300270014671	0.001331996262799057
62-63	0.0	0.0	0.0	1.2285286959566302	0.0013795675578990234
64-65	0.0	0.0	0.0	1.3509296382488436	0.0013795675578990234
66-67	0.0	0.0	0.0	1.4857229029145982	0.0013795675578990234
68-69	0.0	0.0	0.0	1.6368331217996412	0.0013795675578990234
70-71	0.0	0.0	0.0	1.8030472268789235	0.0015222814431989221
72-73	0.0	0.0	0.0	1.9640284894972093	0.001902851803998653
74-75	0.0	0.0	0.0	2.125770892837095	0.002045565689298552
76-77	0.0	0.0	0.0	2.3070175271679663	0.002045565689298552
78-79	0.0	0.0	0.0	2.5135958761395703	0.002045565689298552
80-81	0.0	0.0	0.0	2.7222197908004726	0.002093136984398518
82-83	0.0	0.0	0.0	2.9404531070715683	0.00233099345989835
84-85	0.0	0.0	0.0	3.1631581250820604	0.00233099345989835
86-87	4.7571295099966316E-5	0.0	0.0	3.405605230559039	0.0023547791074483328
88-89	4.7571295099966316E-5	0.0	0.0	3.6755247589562474	0.002378564754998316
90-91	4.7571295099966316E-5	0.0	0.0	3.960286531424646	0.0024023504025482995
92-93	4.7571295099966316E-5	0.0	0.0	4.245405088606294	0.002521278640298215
94-95	4.7571295099966316E-5	0.0	0.0	4.538444266422086	0.0026877781731480975
96-97	4.7571295099966316E-5	0.0	0.0	4.856720016288412	0.0028542777059979794
98-99	9.514259019993263E-5	0.0	0.0	5.207558317650664	0.002996991591297878
100-101	9.514259019993263E-5	0.0	0.0	5.591030527451491	0.002996991591297878
102-103	9.514259019993263E-5	0.0	0.0	5.987846485527861	0.0032586337143476925
104-105	9.514259019993263E-5	0.0	0.0	6.400575041815168	0.0033775619520976085
106-107	9.514259019993263E-5	0.0	0.0	6.841465804801656	0.0033775619520976085
108-109	9.514259019993263E-5	0.0	0.0	7.296294957252434	0.0034489188947475578
110-111	9.514259019993263E-5	0.0	0.0	7.779000888631792	0.003758132312897339
112-113	9.514259019993263E-5	0.0	0.0	8.282804689387987	0.0038532749030972717
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2060	0.0	56.852947	1
GTATCAA	3715	0.0	50.82676	1
GTACATG	10105	0.0	42.102493	1
ACATGGG	10440	0.0	40.92719	3
TACATGG	10560	0.0	40.067074	2
CATGGGG	9485	0.0	38.262486	4
ATGGGGG	5805	0.0	35.622177	5
TCAACGC	5000	0.0	35.278893	4
CAACGCA	5205	0.0	33.087986	5
TATCAAC	5575	0.0	32.70919	2
ATCAACG	5490	0.0	32.238686	3
AACGCAG	5515	0.0	31.01199	5
ATGGGGA	3585	0.0	25.93156	5
TGGGGGG	4190	0.0	25.603743	6
ACGCAGA	6740	0.0	25.280493	7
CGCAGAG	7170	0.0	23.598185	7
AGAGTAC	7455	0.0	22.33243	10-11
CAGAGTA	8035	0.0	22.092646	9
GTAAGTA	915	0.0	21.492456	2
GCAGAGT	8355	0.0	20.9618	8
>>END_MODULE