FastQCFastQC Report
Mon 15 Aug 2016
SRR2921324_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921324_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037075
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.057.02471
GTATCAA34500.051.0527421
TCAACGC45250.038.2657434
CAACGCA46150.037.518585
GTACATG105500.037.1124731
TATCAAC49850.035.212162
ATCAACG49900.035.1768763
AACGCAG50050.034.8302576
TACATGG110150.034.7345962
ACATGGG112900.033.4669083
CATGGGG101550.030.8206394
ACGCAGA61800.028.1089677
ATGGGGG61850.027.8024645
CGCAGAG67050.026.085498
AGAGTAC68350.022.28188510-11
GCAGAGT78050.021.9517979
TGGGGGG39700.021.5806796
GTATAGG6900.021.5594331
GTATACG1450.003866864820.5186351
GTGGTAT12200.020.4849991
Produced by FastQC (version 0.11.5)