FastQCFastQC Report
Mon 15 Aug 2016
SRR2921324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037075
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25020.12282316556827805No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.077.0050661
GTATCAA46100.047.3037721
GTACATG100950.037.0486031
TCAACGC61450.034.2312934
TACATGG107450.034.2175752
ACATGGG109450.033.3200873
TATCAAC65500.032.8425142
CATGGGG94900.032.651844
CAACGCA64300.032.52156
AACGCAG66800.031.6611216
ATCAACG67550.031.3165233
GTGGTAT10950.030.009061
ATGGGGG59550.029.1193525
ACGCAGA82300.025.2570488
CGCAGAG85550.024.2279249
TGGGGGG40150.023.1486
GTAAGTA8600.022.865782
GTATACG1602.5405004E-422.4044931
AGAGTAC86900.022.06844710-11
TACCGTA2704.4919943E-822.0620887
Produced by FastQC (version 0.11.5)