FastQCFastQC Report
Mon 15 Aug 2016
SRR2921323_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921323_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734014
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.059.3557361
GTATCAA33150.052.244951
TCAACGC39950.041.563284
CAACGCA40650.040.9927755
TATCAAC42450.039.956692
GTACATG100400.039.1834451
AACGCAG44300.037.8806656
TACATGG103400.037.5850372
ATCAACG45050.036.9901053
ACATGGG106000.036.270123
CATGGGG96700.034.5269934
ATGGGGG60450.033.571285
ACGCAGA55200.030.284947
CGCAGAG58900.028.4834988
TGGGGGG46450.027.287656
GAGTACA69600.026.0810741
AGTACAT69700.025.7013572
GCAGAGT66000.025.1474959
TACCGTA1501.688592E-423.796857
CAGAGTA62800.021.7872610-11
Produced by FastQC (version 0.11.5)