##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921323_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1734014
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.62171874044846	31.0	31.0	34.0	27.0	34.0
2	30.60181578695443	33.0	31.0	34.0	27.0	34.0
3	30.775073326974294	33.0	31.0	34.0	27.0	34.0
4	34.14886500339674	37.0	35.0	37.0	30.0	37.0
5	34.236126121242386	37.0	35.0	37.0	31.0	37.0
6	34.23690523836601	37.0	35.0	37.0	31.0	37.0
7	34.18611960457066	37.0	35.0	37.0	30.0	37.0
8	34.22302588099058	37.0	35.0	37.0	31.0	37.0
9	35.819899377975034	39.0	37.0	39.0	31.0	39.0
10-11	35.820641298167146	39.0	37.0	39.0	30.5	39.0
12-13	35.73682450084025	39.0	37.0	39.0	30.0	39.0
14-15	37.061380992310326	41.0	38.0	41.0	31.0	41.0
16-17	37.051911345583136	41.0	38.0	41.0	31.0	41.0
18-19	37.044715036902815	41.0	38.0	41.0	31.0	41.0
20-21	37.079494456215464	41.0	38.0	41.0	31.0	41.0
22-23	37.03232182669805	41.0	38.0	41.0	30.0	41.0
24-25	37.02211602674488	41.0	38.0	41.0	30.0	41.0
26-27	36.99304676894189	41.0	38.0	41.0	30.0	41.0
28-29	36.92119123605691	40.0	38.0	41.0	30.0	41.0
30-31	36.8403761445986	40.0	38.0	41.0	30.0	41.0
32-33	36.758126520316445	40.0	38.0	41.0	30.0	41.0
34-35	36.6499659172302	40.0	38.0	41.0	30.0	41.0
36-37	36.53652940518358	40.0	37.0	41.0	30.0	41.0
38-39	36.43557606801329	40.0	37.0	41.0	29.0	41.0
40-41	36.32474132273442	40.0	37.0	41.0	29.0	41.0
42-43	36.12357483849611	40.0	36.5	41.0	28.0	41.0
44-45	35.86064530044163	40.0	36.0	41.0	27.0	41.0
46-47	35.62786517294555	40.0	35.0	41.0	27.0	41.0
48-49	35.40703477595913	40.0	35.0	41.0	26.0	41.0
50-51	35.2211775683472	39.0	35.0	41.0	26.0	41.0
52-53	35.02232969283985	39.0	35.0	41.0	26.0	41.0
54-55	34.80533461667552	39.0	35.0	41.0	25.0	41.0
56-57	34.54903391783458	38.0	34.5	40.0	24.0	41.0
58-59	34.27184469098866	38.0	34.0	40.0	23.5	41.0
60-61	33.97266458056279	37.5	34.0	40.0	23.0	41.0
62-63	33.6326552150098	37.0	34.0	40.0	22.0	41.0
64-65	33.28035442620417	36.0	33.5	39.0	21.5	41.0
66-67	32.93454724125641	36.0	33.0	39.0	21.0	41.0
68-69	32.576380582855734	35.0	33.0	38.5	20.0	40.0
70-71	32.52518578281375	35.0	33.0	38.0	20.5	40.0
72-73	32.234947930062845	35.0	33.0	37.0	20.0	39.5
74-75	31.912271181201536	35.0	33.0	37.0	19.5	39.0
76-77	31.617298937609498	35.0	33.0	36.0	18.5	39.0
78-79	31.341435536275945	35.0	33.0	36.0	18.0	37.5
80-81	31.07414126990901	35.0	32.5	35.0	16.0	37.0
82-83	30.849911534739626	35.0	32.0	35.0	12.5	36.5
84-85	30.63226853993105	35.0	32.0	35.0	9.5	36.0
86-87	30.461379492899134	35.0	32.0	35.0	7.0	36.0
88-89	30.32362772157549	35.0	32.0	35.0	7.0	36.0
90-91	30.192939907059575	35.0	32.0	35.0	3.5	35.0
92-93	30.07213696083192	35.0	32.0	35.0	2.0	35.0
94-95	29.938387175651407	35.0	31.0	35.0	2.0	35.0
96-97	29.832962421295328	35.0	31.0	35.0	2.0	35.0
98-99	29.73729422023121	35.0	31.0	35.0	2.0	35.0
100-101	29.62827808772017	35.0	31.0	35.0	2.0	35.0
102-103	28.75915102184873	33.5	29.5	34.5	2.0	35.0
104-105	29.347009885733332	34.0	31.0	35.0	2.0	35.0
106-107	29.406003642415804	34.0	31.0	35.0	2.0	35.0
108-109	29.326075510347668	34.0	31.0	35.0	2.0	35.0
110-111	29.221209863357505	34.0	31.0	35.0	2.0	35.0
112-113	29.106628896883187	34.0	30.5	35.0	2.0	35.0
114-115	28.964692038241907	34.0	30.0	35.0	2.0	35.0
116-117	28.816244563192683	34.0	30.0	35.0	2.0	35.0
118-119	28.799727107162916	34.0	30.5	35.0	2.0	35.0
120-121	28.94311233934674	35.0	31.0	35.0	2.0	35.0
122-123	28.78511419169626	35.0	31.0	35.0	2.0	35.0
124-125	28.191525558617172	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59695.0
3	8282.0
4	5200.0
5	4453.0
6	4757.0
7	5377.0
8	5785.0
9	6038.0
10	6618.0
11	7169.0
12	7092.0
13	7129.0
14	8008.0
15	6976.0
16	5605.0
17	5295.0
18	5043.0
19	4727.0
20	4733.0
21	5030.0
22	5663.0
23	7482.0
24	10715.0
25	13774.0
26	15081.0
27	16098.0
28	19511.0
29	23398.0
30	28465.0
31	35811.0
32	46289.0
33	62174.0
34	98198.0
35	159671.0
36	335349.0
37	546162.0
38	137107.0
39	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.057626033516655	16.984885494862336	11.608493653984247	25.34899481763676
2	16.79536100549998	18.90872429958289	38.7670836255583	25.528831069358827
3	20.428245146555003	23.255124522730156	28.16607260269232	28.15055772802252
4	13.74170886733034	14.367624496764295	34.02386692659968	37.866799709305674
5	14.5383636684519	37.663609508067	32.43860209683168	15.359424726649419
6	34.620354442763876	32.976433167314475	16.887313690268112	15.51589869965354
7	30.726279538288242	31.694321577520938	19.085147210563562	18.494251673627257
8	28.51207947357523	34.661257381868474	18.494655408986805	18.33200773556949
9	28.739836413697322	15.799712322494097	17.789866231113233	37.67058503269534
10-11	27.272137429234512	25.179336015539572	25.862508478017137	21.686018077208782
12-13	26.699348007255256	22.482849505310238	26.32663654216037	24.491165945274133
14-15	24.429342513297893	23.80232432734825	23.60470863436108	28.163624524992777
16-17	24.84335571294237	26.85307978427222	24.11720790371318	24.18635659907223
18-19	24.750234295024296	26.305376530126733	25.535216344420895	23.409172830428073
20-21	25.55122468582291	25.20164400673728	25.384984265553285	23.86214704188653
22-23	25.60302319009418	24.646554359172516	25.268955494166427	24.481466956566873
24-25	24.979230641241877	24.84367110563349	25.327083157311638	24.850015095812992
26-27	24.49645115208516	25.218822072005825	25.51035414658959	24.77437262931942
28-29	24.772157034519093	25.72061240267382	25.285991402739853	24.221239160067235
30-31	25.50766738439809	25.172620319498623	25.220517141496902	24.099195154606385
32-33	25.01067683799495	25.187735002178623	25.283271435067956	24.51831672475847
34-35	24.928001716366413	25.48590731102307	25.46038674187689	24.12570423073363
36-37	24.906663129376856	25.465718326958044	25.410726927474503	24.216891616190598
38-39	24.958648229659776	25.674720169052033	25.21575588959942	24.15087571168877
40-41	24.935679867120747	25.318457470276627	25.369729022062913	24.376133640539706
42-43	24.76529182479929	25.303180646498934	25.113061040994957	24.818466487706818
44-45	24.34163097653506	25.394283196436255	25.624399477249632	24.63968634977905
46-47	24.417582271811956	25.1981476135919	25.518182864185725	24.866087250410427
48-49	24.374952599316508	25.549163051718725	25.367144427107046	24.70873992185772
50-51	24.461446939709454	25.524495387208873	25.49741814613463	24.516639526947046
52-53	24.66514027342432	25.446570949891434	25.290596244957282	24.597692531726967
54-55	24.18741322060242	25.07516916125144	25.736992281950315	25.000425336195818
56-57	24.476210304849985	25.41167263201726	25.23969059381672	24.87242646931603
58-59	24.385938807560038	25.55713772422627	25.48689174217533	24.570031726038362
60-61	24.988220435022075	25.38215100335364	25.48616297612079	24.143465585503492
62-63	24.703044528902552	25.45313247939652	25.392749418950018	24.451073572750918
64-65	24.482960073404357	25.12100934452238	25.431166978189196	24.964863603884062
66-67	24.491724391289026	25.326955606011936	25.27807879230728	24.90324121039176
68-69	24.179256665259448	25.287153472255863	25.542932875641032	24.990656986843653
70-71	24.219524967357653	25.185790313874033	25.717670236178332	24.877014482589978
72-73	24.259306357914795	25.645390954405233	25.21365968664592	24.881643001034057
74-75	24.160509494631775	25.561613964849695	25.369509620644383	24.908366919874148
76-77	24.13062459845924	25.009544703237584	25.459126178093207	25.40070452020997
78-79	24.02533851684092	25.21458095078263	25.645934366459223	25.114146165917226
80-81	24.29323091678059	25.430249581234214	25.301988593192075	24.974530908793117
82-83	24.465564701750296	25.3569486248892	25.465487997517783	24.71199867584272
84-85	24.434755802683526	25.523444747586982	25.11632185605129	24.925477593678206
86-87	24.019701137890664	25.678408913957472	25.361322098609502	24.940567849542365
88-89	23.887828842932525	25.293673741211848	25.517672329397882	25.300825086457746
90-91	24.386395329926053	25.013899029605508	25.575540937544332	25.0241647029241
92-93	24.412076142887297	25.20188615477958	25.51188132173865	24.874156380594474
94-95	24.339988811414663	25.14060706726416	25.395291566459623	25.124112554861554
96-97	23.993637393119077	25.071508676636018	25.598044386565277	25.33680954367962
98-99	23.89860136835324	25.117523468648006	25.38212789713878	25.601747265859977
100-101	23.84827091406448	25.360273096436746	25.574559675827746	25.21689631367103
102-103	23.698795569837802	25.19801814604033	25.670008973823656	25.43317731029821
104-105	23.860651504477808	25.265572838283667	25.495684540501824	25.378091116736705
106-107	23.9593369077283	25.105621323871848	25.616029468717027	25.319012299682825
108-109	23.811844457331137	25.2055678575064	25.593648829110283	25.388938856052178
110-111	23.674311184522985	25.63163226494254	25.534192994350025	25.159863556184455
112-113	23.542325272379088	25.673605317847937	25.568209179758522	25.215860230014453
114-115	23.68706903536876	25.76746415098039	25.453214827100872	25.092251986549968
116-117	23.429046640556923	25.62269206436354	25.541461957377408	25.40679933770213
118-119	23.445085777169076	25.41762426218583	25.643231312052407	25.494058648592695
120-121	23.367738422887303	25.5379640246111	25.820975492717945	25.27332205978365
122-123	23.299279662348756	25.738698605547878	25.562052816687174	25.399968915416192
124-125	23.164418515663815	25.52272069956957	25.566838842766686	25.746021941999924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	376.0
1	332.0
2	279.5
3	239.0
4	208.0
5	196.0
6	192.5
7	245.5
8	340.5
9	414.5
10	415.5
11	403.5
12	455.5
13	503.0
14	514.5
15	561.5
16	618.0
17	674.5
18	735.0
19	764.0
20	797.5
21	876.0
22	982.0
23	1125.5
24	1279.0
25	1577.5
26	2128.5
27	2801.0
28	3599.0
29	4692.5
30	6278.5
31	8014.5
32	10552.0
33	13355.5
34	16072.5
35	19354.5
36	23094.5
37	26778.5
38	29592.5
39	32772.5
40	36325.5
41	39429.5
42	42523.0
43	46717.0
44	51741.5
45	56944.0
46	61937.0
47	66995.5
48	72266.5
49	76746.5
50	80123.5
51	82787.0
52	83319.0
53	82111.5
54	81353.0
55	79312.0
56	74824.5
57	68689.5
58	62374.5
59	55172.5
60	47078.5
61	39731.5
62	32597.5
63	25087.5
64	18388.0
65	13637.5
66	10244.5
67	7730.5
68	5947.0
69	4516.0
70	3370.0
71	2568.5
72	1953.0
73	1484.0
74	1121.0
75	788.0
76	583.0
77	405.0
78	251.5
79	181.5
80	123.0
81	100.0
82	83.5
83	64.0
84	52.0
85	42.0
86	35.0
87	24.0
88	18.0
89	20.0
90	19.5
91	16.0
92	13.0
93	9.5
94	7.5
95	9.0
96	6.5
97	3.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01435974565372598
2	0.010957235639389301
3	0.011187914284429076
4	0.013494700734826824
5	0.00922714580159099
6	0.027450758759733195
7	0.011822280558288458
8	0.011822280558288458
9	0.007381716641272792
10-11	0.0068050200286733555
12-13	0.005709296464734425
14-15	0.005363278497174763
16-17	0.004008041457566086
18-19	0.005132599852134989
20-21	0.004584738070165523
22-23	0.00484425154583527
24-25	0.00516143468276496
26-27	0.0040945459494560014
28-29	0.00516143468276496
30-31	0.00452706840890558
32-33	0.006949194181823214
34-35	0.006833854859303328
36-37	0.006141818924184003
38-39	0.006343662738593806
40-41	0.0062859930773338625
42-43	0.006084149262924059
44-45	0.006228323416073919
46-47	0.008217926729541976
48-49	0.007237542488122933
50-51	0.0049307560377251855
52-53	0.004555903239535552
54-55	0.005276774005284848
56-57	0.006459002061113693
58-59	0.006170653754813974
60-61	0.004555903239535552
62-63	0.00484425154583527
64-65	0.0032871706918167903
66-67	0.0036331886593764526
68-69	0.005651626803474481
70-71	0.004036876288196058
72-73	0.00374852798189634
74-75	0.003489014506226593
76-77	0.003921536965676172
78-79	0.0030564920467770155
80-81	0.0028546482323672127
82-83	0.004671242562055439
84-85	0.008044917745762145
86-87	0.0059976447710341435
88-89	0.004296389763865805
90-91	0.004613572900795495
92-93	0.006689680706153469
94-95	0.0059976447710341435
96-97	0.007698899778202483
98-99	0.006055314432294088
100-101	0.006978029012453186
102-103	0.004728912223315383
104-105	0.004238720102605861
106-107	0.006199488585443946
108-109	0.00562279197284451
110-111	0.006545506553003609
112-113	0.004584738070165523
114-115	0.0023356212810277193
116-117	0.002941152724257128
118-119	0.14206921051387128
120-121	0.009342485124110877
122-123	0.0020472729747280007
124-125	0.0019319336522081135
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1734014.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.61354185704897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83537921959352	48.99718641857258
2	10.162490693223205	12.319691100111106
3	3.3834859611822097	6.152552037925663
4	1.6444558240720566	3.9870516769783824
5	0.9417625125270748	2.8541780736229723
6	0.6033002870201395	2.1940900319798926
7	0.45566483451683887	1.933362166384019
8	0.31445287680495393	1.524808208822923
9	0.24520077965666684	1.3376238948990375
>10	1.335668019727943	14.450234308791208
>50	0.061110132713074486	2.435337252903848
>100	0.01655330591315192	1.5394712607898278
>500	1.9022121962642305E-4	0.07431073307432726
>1k	2.8533182943963454E-4	0.20010283514429006
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	5.766966125994369E-5
14-15	0.0	0.0	0.0	0.0	1.4417415314985923E-4
16-17	0.0	0.0	0.0	0.0	1.7300898377983108E-4
18-19	0.0	0.0	0.0	2.8834830629971846E-5	1.7300898377983108E-4
20-21	0.0	0.0	0.0	8.650449188991554E-5	1.7300898377983108E-4
22-23	0.0	0.0	0.0	2.0184381440980294E-4	2.0184381440980294E-4
24-25	0.0	0.0	0.0	4.6135729007954954E-4	2.8834830629971846E-4
26-27	0.0	0.0	0.0	0.0010668887333089583	2.8834830629971846E-4
28-29	0.0	0.0	0.0	0.0046712425620554394	3.171831369296903E-4
30-31	0.0	0.0	0.0	0.018684970248221758	3.4601796755966217E-4
32-33	0.0	0.0	0.0	0.04607805934669501	4.0368762881960583E-4
34-35	0.0	0.0	0.0	0.08044917745762145	4.0368762881960583E-4
36-37	0.0	0.0	0.0	0.12894936257723408	4.0368762881960583E-4
38-39	0.0	0.0	0.0	0.20763961536642725	4.0368762881960583E-4
40-41	0.0	0.0	0.0	0.3042651328074629	4.0368762881960583E-4
42-43	0.0	0.0	0.0	0.38655973942540256	4.613572900795495E-4
44-45	0.0	0.0	0.0	0.476236062684615	5.190269513394932E-4
46-47	0.0	0.0	0.0	0.5693437307887941	5.190269513394932E-4
48-49	0.0	0.0	0.0	0.6740718356368518	5.190269513394932E-4
50-51	0.0	0.0	0.0	0.7845380717802739	5.190269513394932E-4
52-53	0.0	0.0	0.0	0.9042891233865471	5.478617819694651E-4
54-55	0.0	0.0	0.0	1.036323812841188	5.766966125994369E-4
56-57	0.0	0.0	0.0	1.166455403474251	5.766966125994369E-4
58-59	0.0	0.0	0.0	1.3127921689213582	6.055314432294088E-4
60-61	0.0	0.0	0.0	1.4640885252368205	6.920359351193243E-4
62-63	0.0	0.0	0.0	1.6379625539355507	6.920359351193243E-4
64-65	0.0	0.0	0.0	1.800302650382292	6.920359351193243E-4
66-67	0.0	0.0	0.0	1.9774350149422093	6.920359351193243E-4
68-69	0.0	0.0	0.0	2.1787309675700426	6.920359351193243E-4
70-71	0.0	0.0	0.0	2.3801999291816562	7.497055963792679E-4
72-73	0.0	0.0	0.0	2.5732202854186874	8.650449188991554E-4
74-75	0.0	0.0	0.0	2.7750064301672306	8.650449188991554E-4
76-77	0.0	0.0	0.0	3.0194681242481316	8.650449188991554E-4
78-79	0.0	0.0	0.0	3.2891891299608886	8.650449188991554E-4
80-81	0.0	0.0	0.0	3.5439448585766895	8.938797495291272E-4
82-83	0.0	0.0	0.0	3.8054767723905343	0.0010380539026789864
84-85	0.0	0.0	0.0	4.078427279133848	0.0010380539026789864
86-87	5.766966125994369E-5	0.0	0.0	4.386642783737617	0.0010380539026789864
88-89	1.1533932251988739E-4	0.0	0.0	4.734362006304448	0.0010668887333089583
90-91	1.1533932251988739E-4	0.0	0.0	5.089030423053101	0.0010957235639389301
92-93	1.1533932251988739E-4	0.0	0.0	5.430752000848898	0.0010957235639389301
94-95	1.1533932251988739E-4	0.0	0.0	5.781066358172425	0.0011533932251988738
96-97	1.1533932251988739E-4	0.0	0.0	6.171172781765314	0.0011533932251988738
98-99	1.1533932251988739E-4	0.0	0.0	6.614883155499321	0.0011533932251988738
100-101	1.1533932251988739E-4	0.0	0.0	7.082670612809355	0.0011533932251988738
102-103	1.1533932251988739E-4	0.0	0.0	7.562020837202006	0.0011533932251988738
104-105	1.1533932251988739E-4	0.0	0.0	8.052357132064678	0.0011533932251988738
106-107	1.1533932251988739E-4	0.0	0.0	8.577006875377016	0.0012110628864588175
108-109	1.1533932251988739E-4	0.0	0.0	9.134816673913821	0.001297567378348733
110-111	1.1533932251988739E-4	0.0	0.0	9.7291602028588	0.001326402208978705
112-113	1.1533932251988739E-4	0.0	0.0	10.315228135412978	0.0013552370396086768
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1855	0.0	59.355736	1
GTATCAA	3315	0.0	52.24495	1
TCAACGC	3995	0.0	41.56328	4
CAACGCA	4065	0.0	40.992775	5
TATCAAC	4245	0.0	39.95669	2
GTACATG	10040	0.0	39.183445	1
AACGCAG	4430	0.0	37.880665	6
TACATGG	10340	0.0	37.585037	2
ATCAACG	4505	0.0	36.990105	3
ACATGGG	10600	0.0	36.27012	3
CATGGGG	9670	0.0	34.526993	4
ATGGGGG	6045	0.0	33.57128	5
ACGCAGA	5520	0.0	30.28494	7
CGCAGAG	5890	0.0	28.483498	8
TGGGGGG	4645	0.0	27.28765	6
GAGTACA	6960	0.0	26.081074	1
AGTACAT	6970	0.0	25.701357	2
GCAGAGT	6600	0.0	25.147495	9
TACCGTA	150	1.688592E-4	23.79685	7
CAGAGTA	6280	0.0	21.78726	10-11
>>END_MODULE