FastQCFastQC Report
Mon 15 Aug 2016
SRR2921323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734014
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26060.15028713724341325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19600.11303253606948964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17440.1005758892373418No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.068.5402151
GTATCAA40400.050.5446431
GTACATG96550.040.3204841
TACATGG98050.039.6233752
ACATGGG101550.038.140373
CATGGGG89100.036.9827274
TCAACGC56900.034.6630864
TATCAAC59350.034.135772
CAACGCA59000.033.6313065
ATGGGGG54100.033.262985
ATCAACG60200.032.7629473
AACGCAG61650.032.675556
TGGGGGG40600.029.2123376
ACGCAGA75000.026.5376077
GTATACG1159.847878E-425.9598731
CGCAGAG78500.025.196038
GTAAGTA9250.025.1231652
GTGGTAT10600.024.2210521
TGTAAGT10250.023.3005711
GTCTTAG5950.023.0802921
Produced by FastQC (version 0.11.5)