##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921323_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1734014
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.33163919091772	33.0	30.0	34.0	19.0	34.0
2	30.07523699347295	33.0	31.0	34.0	26.0	34.0
3	30.309262208955637	33.0	31.0	34.0	27.0	34.0
4	32.48439055278677	37.0	35.0	37.0	19.0	37.0
5	32.159524086887416	37.0	33.0	37.0	19.0	37.0
6	32.321209055982244	37.0	35.0	37.0	17.0	37.0
7	32.527399432761214	37.0	35.0	37.0	17.0	37.0
8	32.77921746883243	37.0	35.0	37.0	17.0	37.0
9	34.52863587029862	39.0	37.0	39.0	17.0	39.0
10-11	34.70683685368169	39.0	37.0	39.0	25.5	39.0
12-13	34.73134818980701	39.0	37.0	39.0	25.5	39.0
14-15	36.112636922193246	41.0	38.0	41.0	27.0	41.0
16-17	36.141061721531656	41.0	38.0	41.0	27.0	41.0
18-19	36.17320102375183	41.0	38.0	41.0	27.0	41.0
20-21	36.174802510245016	41.0	38.0	41.0	27.0	41.0
22-23	36.12550331196864	41.0	38.0	41.0	26.5	41.0
24-25	36.067017336653564	41.0	38.0	41.0	26.0	41.0
26-27	35.975137167289304	41.0	38.0	41.0	25.0	41.0
28-29	35.902869007978026	40.0	38.0	41.0	25.0	41.0
30-31	35.80380089203432	40.0	37.0	41.0	24.5	41.0
32-33	35.67123506499948	40.0	37.0	41.0	24.0	41.0
34-35	35.58749814015343	40.0	37.0	41.0	23.5	41.0
36-37	35.50489413580283	40.0	36.5	41.0	22.5	41.0
38-39	35.4091434671231	40.0	36.0	41.0	21.5	41.0
40-41	35.2735309518839	40.0	36.0	41.0	19.5	41.0
42-43	35.125801175769055	40.0	35.5	41.0	17.0	41.0
44-45	34.972726287100336	40.0	35.0	41.0	14.5	41.0
46-47	34.74928950977328	40.0	35.0	41.0	8.5	41.0
48-49	34.51904684737263	40.0	35.0	41.0	6.0	41.0
50-51	34.32781482733127	40.0	35.0	41.0	2.0	41.0
52-53	34.138869697707165	39.0	35.0	41.0	2.0	41.0
54-55	33.93119202036431	39.0	35.0	41.0	2.0	41.0
56-57	33.6852104423609	39.0	35.0	41.0	2.0	41.0
58-59	33.608106393604665	38.5	35.0	41.0	2.0	41.0
60-61	33.355233291080694	37.5	34.5	41.0	2.0	41.0
62-63	33.06127776361667	37.0	34.0	40.0	2.0	41.0
64-65	32.7553874997549	36.5	34.0	40.0	2.0	41.0
66-67	32.43313433455555	36.0	34.0	39.0	2.0	41.0
68-69	32.094484242918455	35.5	34.0	39.0	2.0	41.0
70-71	31.764444520055775	35.0	33.5	38.0	2.0	40.5
72-73	31.42744637586548	35.0	33.0	37.0	2.0	39.5
74-75	31.0966422416428	35.0	33.0	37.0	2.0	39.0
76-77	30.807082872456625	35.0	33.0	36.0	2.0	39.0
78-79	30.556716670107622	35.0	33.0	36.0	2.0	37.0
80-81	30.339335207212862	35.0	33.0	35.0	2.0	37.0
82-83	30.147419224988955	35.0	33.0	35.0	2.0	36.5
84-85	29.98156127920536	35.0	32.0	35.0	2.0	36.0
86-87	29.847276896264965	35.0	32.0	35.0	2.0	36.0
88-89	29.735584314774854	35.0	32.0	35.0	2.0	36.0
90-91	29.638703897431046	35.0	32.0	35.0	2.0	35.0
92-93	29.572918096393686	35.0	32.0	35.0	2.0	35.0
94-95	29.506551850215743	35.0	32.0	35.0	2.0	35.0
96-97	29.441678671567818	35.0	32.0	35.0	2.0	35.0
98-99	29.390313169328508	35.0	32.0	35.0	2.0	35.0
100-101	29.335310729901835	35.0	32.0	35.0	2.0	35.0
102-103	28.659992364536848	34.0	30.0	35.0	2.0	35.0
104-105	29.201815844624093	35.0	31.0	35.0	2.0	35.0
106-107	29.25877818748868	35.0	32.0	35.0	2.0	35.0
108-109	29.331035389564327	35.0	32.0	35.0	2.0	35.0
110-111	29.440322281134986	35.0	32.0	35.0	2.0	35.0
112-113	29.399523302580025	35.0	32.0	35.0	2.0	35.0
114-115	29.33387879221275	35.0	32.0	35.0	2.0	35.0
116-117	29.265104549328896	35.0	32.0	35.0	2.0	35.0
118-119	29.181980941330345	35.0	32.0	35.0	2.0	35.0
120-121	29.081014051789662	35.0	32.0	35.0	2.0	35.0
122-123	28.962920426248	35.0	32.0	35.0	2.0	35.0
124-125	28.438155343613143	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	112766.0
3	6927.0
4	5327.0
5	5097.0
6	5329.0
7	5769.0
8	5935.0
9	5926.0
10	6020.0
11	6099.0
12	6748.0
13	8157.0
14	9937.0
15	8722.0
16	5750.0
17	4999.0
18	4525.0
19	4052.0
20	3673.0
21	3900.0
22	4433.0
23	5479.0
24	7253.0
25	9480.0
26	10841.0
27	12488.0
28	15059.0
29	18008.0
30	21752.0
31	26904.0
32	35336.0
33	48082.0
34	74394.0
35	127370.0
36	288794.0
37	606535.0
38	195912.0
39	236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2700312289199	17.170294227567304	11.442408155850707	27.117266387662088
2	16.237758172656044	19.12014551208929	37.7520596719519	26.890036643302768
3	19.591018296276733	22.878073648771004	28.2667844665614	29.264123588390866
4	13.666624368410124	15.507521399072516	31.025425097478255	39.8004291350391
5	14.609799209778416	37.59121051780309	31.806129993761406	15.992860278657087
6	34.890068679670264	33.20065494927489	16.26163123448015	15.647645136574695
7	30.66713888618106	31.929672363102966	18.057954947258885	19.345233803457084
8	29.137289166875735	34.50906773478209	17.488544577382136	18.865098520960036
9	28.737526104130467	15.727590818728189	17.462733307918874	38.07214976922248
10-11	27.58192136010796	25.299167233762788	24.941694829236784	22.177216576892466
12-13	27.09895275239337	22.649174648402585	25.738754162533706	24.513118436670343
14-15	24.892186568274536	23.857448671117996	23.209904879660716	28.04045988094675
16-17	24.836333501344278	26.81140406017483	23.87547044026173	24.47679199821916
18-19	24.818542410845588	25.79082406485761	25.713344874954874	23.677288649341932
20-21	25.480936140077297	24.787862151055297	25.722399011772684	24.008802697094715
22-23	25.300631944148087	24.295478583217896	25.63439510869001	24.769494363944005
24-25	24.81718717380598	24.59022822191747	25.630877259353152	24.961707344923397
26-27	24.39766922297052	24.819147942288815	25.879981361165484	24.903201473575184
28-29	24.545447729949124	25.317125467268433	25.681107534310566	24.456319268471884
30-31	25.26565529459393	24.883247770779242	25.63393375139993	24.217163183226894
32-33	24.811622051494396	24.97272225022405	25.678887252352055	24.536768445929503
34-35	24.83543962159475	25.11161962936862	25.91045977714136	24.142480971895267
36-37	24.91963732703427	24.970761481741206	25.90129030100103	24.20831089022349
38-39	24.758335284490208	25.29578769260225	25.77882877531554	24.167048247592003
40-41	24.696830590756477	25.191607449536164	25.80417459143928	24.307387368268078
42-43	24.577598566101543	25.126325392989905	25.485953400606913	24.810122640301635
44-45	24.213126306938697	25.298873019479657	25.878510784803353	24.609489888778292
46-47	24.300380504424993	25.180852057711185	25.451351603850945	25.067415834012873
48-49	24.379906967302457	25.529320985874392	25.265453450779518	24.82531859604363
50-51	24.381291039172694	25.54065307431197	25.429696646047837	24.648359240467492
52-53	24.56920763038822	25.39927589973322	25.294288281409493	24.737228188469064
54-55	24.147440562763624	25.092040779370873	25.734163622669715	25.026355035195795
56-57	24.317739072464235	25.384310622636264	25.239905790841366	25.058044514058132
58-59	24.31453840626431	25.462222334998447	25.457810605912062	24.76542865282518
60-61	24.761074593400053	25.31057996071543	25.586385115691108	24.341960330193412
62-63	24.532385551673748	25.382148010339016	25.48929824095999	24.596168197027243
64-65	24.32523612842803	25.01415790183932	25.485578547808725	25.17502742192393
66-67	24.329042326071185	25.335089566750902	25.330562498341997	25.005305608835915
68-69	24.012061032955906	25.24336597051696	25.561558326518703	25.18301467000843
70-71	24.10600491114835	25.165252414340365	25.649216211633817	25.07952646287746
72-73	24.186857776234795	25.619833519221874	25.11464728658477	25.078661417958564
74-75	24.029275426842	25.576033411494947	25.29042441410508	25.10426674755798
76-77	24.07944803213815	25.09982618364096	25.261877931781406	25.558847852439488
78-79	24.012464720584724	25.225430705865122	25.477879648030523	25.284224925519634
80-81	24.18997193794283	25.42194007660838	25.15288227200011	25.235205713448682
82-83	24.349399716495945	25.371334948852777	25.23015961808844	25.049105716562842
84-85	24.240231047730873	25.538057939555276	24.936621042275323	25.28508997043853
86-87	23.82945005057629	25.805702837462675	25.10152743864813	25.2633196733129
88-89	23.72792261192816	25.377188419470663	25.282292991867422	25.612595976733754
90-91	24.140588821152395	25.12230342784083	25.346696985652308	25.390410765354467
92-93	24.053260546111773	25.353430557253613	25.292386868773082	25.300922027861528
94-95	24.034507278212192	25.207654392042496	25.12484061519794	25.63299771454737
96-97	23.748337301510634	25.09778671150318	25.26381867164628	25.890057315339902
98-99	23.624794047103453	25.294764226104416	25.112037799024574	25.968403927767554
100-101	23.606499140145353	25.451466943173468	25.229928939443397	25.712104977237786
102-103	23.420341473598253	25.31960526270261	25.293711584796892	25.966341678902243
104-105	23.60655520261682	25.404437800891806	25.09267525253574	25.896331743955635
106-107	23.73503359412598	25.21804247211014	25.16896543471384	25.877958499050035
108-109	23.562899382844382	25.275237333719335	25.228899480306698	25.932963803129578
110-111	23.434142521173563	25.677075343627052	25.145588067372472	25.743194067826913
112-113	23.350639202190315	25.741155912854545	25.160964764943433	25.747240120011703
114-115	23.35605915557288	25.832639371074116	24.973817822796256	25.83748365055675
116-117	23.141038968004317	25.600757093643917	25.119874602039964	26.138329336311806
118-119	23.287209873325086	25.39908322090097	25.20418764531024	26.10951926046371
120-121	23.175891041060293	25.540697262012756	25.253819035750062	26.029592661176892
122-123	22.991965323786147	25.750641297728237	25.022347380367986	26.235045998117627
124-125	22.850540495262	25.66070063549175	24.905659506871782	26.58309936237447
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	82.0
1	76.0
2	82.0
3	86.0
4	84.5
5	109.5
6	218.0
7	525.0
8	783.0
9	917.5
10	867.5
11	720.0
12	743.0
13	815.5
14	913.5
15	968.0
16	1023.0
17	1133.5
18	1222.0
19	1259.0
20	1279.0
21	1338.0
22	1495.5
23	1664.5
24	1788.5
25	2094.0
26	2606.0
27	3216.5
28	3836.5
29	4710.5
30	6125.0
31	7752.0
32	9914.0
33	12304.5
34	14713.5
35	17582.5
36	21220.5
37	24992.5
38	27872.0
39	31092.5
40	34542.5
41	37327.0
42	40320.5
43	45032.0
44	50215.5
45	55328.5
46	60275.5
47	65139.5
48	70986.5
49	75795.5
50	79912.5
51	83760.5
52	84536.5
53	83796.5
54	83002.5
55	80416.5
56	76066.0
57	70075.0
58	63747.0
59	56705.5
60	48512.0
61	40724.0
62	33419.5
63	25818.5
64	18863.0
65	13903.5
66	10596.0
67	8163.0
68	6139.0
69	4591.0
70	3542.5
71	2747.0
72	2090.5
73	1681.5
74	1291.0
75	929.5
76	679.0
77	455.0
78	323.0
79	263.5
80	219.0
81	197.0
82	180.0
83	159.0
84	143.0
85	129.5
86	132.0
87	119.0
88	104.5
89	100.5
90	92.0
91	83.0
92	80.0
93	76.0
94	68.0
95	67.0
96	59.0
97	47.0
98	37.5
99	18.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.316491100994571
2	0.0
3	0.0
4	0.016954880410423444
5	0.1645892132358793
6	0.043252245944957766
7	0.0205880690697999
8	0.006862689689933299
9	0.005363278497174763
10-11	0.001960768482838086
12-13	0.057381312953643966
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	4.0368762881960583E-4
92-93	5.766966125994369E-4
94-95	1.7300898377983108E-4
96-97	6.055314432294088E-4
98-99	5.7669661259943686E-5
100-101	0.0
102-103	0.0
104-105	1.1533932251988737E-4
106-107	3.171831369296903E-4
108-109	6.055314432294088E-4
110-111	6.920359351193243E-4
112-113	9.515494107890709E-4
114-115	5.766966125994369E-4
116-117	8.362100882691836E-4
118-119	4.901921207095215E-4
120-121	1.7300898377983108E-4
122-123	0.0017300898377983107
124-125	1.4417415314985923E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1734014.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.45409702897569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.82617362272808	51.72833883972646
2	9.005901382464183	11.249108775476087
3	3.050169325644088	5.714867130557437
4	1.470386411464101	3.6732662244666527
5	0.8513212234083631	2.65842491447861
6	0.549083858374993	2.0575521940797694
7	0.40669222651410053	1.7779717042949252
8	0.301479386492433	1.5062898284987576
9	0.2115538560953539	1.1891164549889872
>10	1.2516818748889935	13.876879656622352
>50	0.05791975373792326	2.378853630721992
>100	0.01699085807515381	1.6067759580267804
>500	1.846343177784147E-4	0.0773164921506464
>1k	4.6158579444603666E-4	0.5052381959105998
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2606	0.15028713724341325	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1960	0.11303253606948964	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1744	0.1005758892373418	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7300898377983108E-4
2	0.0	0.0	0.0	0.0	1.7300898377983108E-4
3	0.0	0.0	0.0	0.0	1.7300898377983108E-4
4	0.0	0.0	0.0	0.0	1.7300898377983108E-4
5	0.0	0.0	0.0	0.0	1.7300898377983108E-4
6	0.0	0.0	0.0	0.0	1.7300898377983108E-4
7	0.0	0.0	0.0	0.0	1.7300898377983108E-4
8	0.0	0.0	0.0	0.0	1.7300898377983108E-4
9	0.0	0.0	0.0	0.0	1.7300898377983108E-4
10-11	0.0	0.0	0.0	0.0	1.7300898377983108E-4
12-13	0.0	0.0	0.0	0.0	1.7300898377983108E-4
14-15	0.0	0.0	0.0	0.0	2.595134756697466E-4
16-17	0.0	0.0	0.0	0.0	3.4601796755966217E-4
18-19	0.0	0.0	0.0	2.8834830629971846E-5	3.74852798189634E-4
20-21	0.0	0.0	0.0	1.4417415314985923E-4	4.0368762881960583E-4
22-23	0.0	0.0	0.0	2.595134756697466E-4	4.325224594495777E-4
24-25	0.0	0.0	0.0	4.6135729007954954E-4	4.6135729007954954E-4
26-27	0.0	0.0	0.0	0.0010668887333089583	4.6135729007954954E-4
28-29	0.0	0.0	0.0	0.00420988527197589	4.6135729007954954E-4
30-31	0.0	0.0	0.0	0.01776225566806266	4.6135729007954954E-4
32-33	0.0	0.0	0.0	0.04388661221881715	4.6135729007954954E-4
34-35	0.0	0.0	0.0	0.07690249329013492	4.6135729007954954E-4
36-37	0.0	0.0	0.0	0.12266336949990023	4.6135729007954954E-4
38-39	0.0	0.0	0.0	0.1975762594765671	4.6135729007954954E-4
40-41	0.0	0.0	0.0	0.2893575253717675	4.6135729007954954E-4
42-43	0.0	0.0	0.0	0.36692321976639175	4.901921207095213E-4
44-45	0.0	0.0	0.0	0.4516976218185089	5.190269513394932E-4
46-47	0.0	0.0	0.0	0.5396726900705531	5.190269513394932E-4
48-49	0.0	0.0	0.0	0.6386338287926164	5.190269513394932E-4
50-51	0.0	0.0	0.0	0.7428140718587047	5.190269513394932E-4
52-53	0.0	0.0	0.0	0.8566251483552036	5.190269513394932E-4
54-55	0.0	0.0	0.0	0.982258505409991	5.190269513394932E-4
56-57	0.0	0.0	0.0	1.1048353704180012	5.190269513394932E-4
58-59	0.0	0.0	0.0	1.2447996382958846	5.190269513394932E-4
60-61	0.0	0.0	0.0	1.3880510768655847	5.190269513394932E-4
62-63	0.0	0.0	0.0	1.5552354248581615	5.190269513394932E-4
64-65	0.0	0.0	0.0	1.709299924914101	5.190269513394932E-4
66-67	0.0	0.0	0.0	1.8776376661318768	5.190269513394932E-4
68-69	0.0	0.0	0.0	2.0678610437978007	5.190269513394932E-4
70-71	0.0	0.0	0.0	2.2601316944384533	5.190269513394932E-4
72-73	0.0	0.0	0.0	2.4411279263027863	8.650449188991554E-4
74-75	0.0	0.0	0.0	2.6325623668551694	8.650449188991554E-4
76-77	0.0	0.0	0.0	2.8650864410552623	8.650449188991554E-4
78-79	0.0	0.0	0.0	3.121312746033192	8.650449188991554E-4
80-81	0.0	0.0	0.0	3.362343095269127	9.22714580159099E-4
82-83	0.0	0.0	0.0	3.6107551611463347	0.0010380539026789864
84-85	0.0	0.0	0.0	3.8679906851963133	0.0010380539026789864
86-87	0.0	0.0	0.0	4.163461194661634	0.0010380539026789864
88-89	0.0	0.0	0.0	4.491774576214494	0.0010957235639389301
90-91	0.0	0.0	0.0	4.831131697898632	0.0010957235639389301
92-93	0.0	0.0	0.0	5.156042569437155	0.0011533932251988738
94-95	0.0	0.0	0.0	5.487412443036792	0.001297567378348733
96-97	0.0	0.0	0.0	5.85831486943012	0.0013552370396086768
98-99	0.0	0.0	0.0	6.282475227997006	0.0013840718702386487
100-101	0.0	0.0	0.0	6.730828009462438	0.0013840718702386487
102-103	0.0	0.0	0.0	7.187917744608752	0.0013840718702386487
104-105	0.0	0.0	0.0	7.6551861749674455	0.0014129067008686205
106-107	0.0	0.0	0.0	8.158901831242424	0.0014417415314985924
108-109	0.0	0.0	0.0	8.690558438397844	0.001470576362128564
110-111	0.0	0.0	0.0	9.256528494002932	0.0015282460233885077
112-113	0.0	0.0	0.0	9.818259829505413	0.0016147505152784233
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2535	0.0	68.540215	1
GTATCAA	4040	0.0	50.544643	1
GTACATG	9655	0.0	40.320484	1
TACATGG	9805	0.0	39.623375	2
ACATGGG	10155	0.0	38.14037	3
CATGGGG	8910	0.0	36.982727	4
TCAACGC	5690	0.0	34.663086	4
TATCAAC	5935	0.0	34.13577	2
CAACGCA	5900	0.0	33.631306	5
ATGGGGG	5410	0.0	33.26298	5
ATCAACG	6020	0.0	32.762947	3
AACGCAG	6165	0.0	32.67555	6
TGGGGGG	4060	0.0	29.212337	6
ACGCAGA	7500	0.0	26.537607	7
GTATACG	115	9.847878E-4	25.959873	1
CGCAGAG	7850	0.0	25.19603	8
GTAAGTA	925	0.0	25.123165	2
GTGGTAT	1060	0.0	24.221052	1
TGTAAGT	1025	0.0	23.300571	1
GTCTTAG	595	0.0	23.080292	1
>>END_MODULE