FastQCFastQC Report
Mon 15 Aug 2016
SRR2921322_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921322_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616194
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.058.7847251
GTATCAA28250.052.2428131
GTACATG94700.042.229181
TCAACGC35200.041.419354
TACATGG97100.040.6938442
CAACGCA36050.040.6078225
ACATGGG99200.039.892373
AACGCAG38350.038.6355366
ATCAACG38800.037.729693
TATCAAC39150.037.3923872
CATGGGG85500.037.375594
ATGGGGG57650.036.9541855
TGGGGGG44750.031.7802896
ACGCAGA49050.030.1999237
CGCAGAG51700.028.5368928
GTGGTAT10050.027.2386191
GAGTACA65300.026.1554411
AGTACAT63950.025.962322
TGGTATC10350.024.7233872
GCAGAGT59700.024.5135549
Produced by FastQC (version 0.11.5)