##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921322_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1616194 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.601287345454814 31.0 31.0 34.0 28.0 34.0 2 30.75503064607343 31.0 31.0 34.0 27.0 34.0 3 30.83574187257223 33.0 31.0 34.0 27.0 34.0 4 34.3174309519773 37.0 35.0 37.0 32.0 37.0 5 34.38614052520923 37.0 35.0 37.0 32.0 37.0 6 34.61182073439203 37.0 35.0 37.0 32.0 37.0 7 34.6345599600048 37.0 35.0 37.0 32.0 37.0 8 34.62973133175844 37.0 35.0 37.0 32.0 37.0 9 36.27674029231639 39.0 37.0 39.0 32.0 39.0 10-11 36.280834788397925 39.0 37.0 39.0 32.0 39.0 12-13 36.19526028434706 39.0 37.0 39.0 32.0 39.0 14-15 37.55086982132096 41.0 38.0 41.0 32.0 41.0 16-17 37.53995745560248 41.0 38.0 41.0 32.0 41.0 18-19 37.53796945168711 41.0 38.0 41.0 32.0 41.0 20-21 37.57134477667904 41.0 38.0 41.0 32.0 41.0 22-23 37.53665092185716 41.0 38.0 41.0 32.0 41.0 24-25 37.52613176388478 41.0 38.0 41.0 32.0 41.0 26-27 37.4976407535234 41.0 38.0 41.0 32.0 41.0 28-29 37.428640682987314 41.0 38.0 41.0 31.0 41.0 30-31 37.36145351362522 40.0 38.0 41.0 31.0 41.0 32-33 37.28120850590956 40.0 38.0 41.0 31.0 41.0 34-35 37.177972137008304 40.0 38.0 41.0 30.0 41.0 36-37 37.07646947086798 40.0 38.0 41.0 30.0 41.0 38-39 36.98131783684384 40.0 38.0 41.0 30.0 41.0 40-41 36.88440001633467 40.0 37.5 41.0 30.0 41.0 42-43 36.70495219014549 40.0 37.0 41.0 30.0 41.0 44-45 36.48389333211236 40.0 36.5 41.0 30.0 41.0 46-47 36.279297534825645 40.0 36.0 41.0 29.0 41.0 48-49 36.07871270404419 40.0 36.0 41.0 29.0 41.0 50-51 35.90290119874223 40.0 35.0 41.0 28.0 41.0 52-53 35.72169089849362 39.0 35.0 41.0 28.0 41.0 54-55 35.517588544444536 39.0 35.0 41.0 28.0 41.0 56-57 35.274821587012454 39.0 35.0 41.0 27.5 41.0 58-59 35.0110129105788 38.0 35.0 40.0 27.0 41.0 60-61 34.7202312964904 38.0 34.5 40.0 26.5 41.0 62-63 34.3778141732985 37.0 34.0 40.0 26.0 41.0 64-65 34.0345447390598 37.0 34.0 39.5 26.0 41.0 66-67 33.69458740720483 36.0 34.0 39.0 26.0 41.0 68-69 33.33130985512878 36.0 34.0 39.0 25.0 40.0 70-71 33.27133902241934 35.5 34.0 39.0 26.0 40.0 72-73 32.97344316338261 35.0 34.0 37.5 25.5 39.5 74-75 32.63839242071187 35.0 33.5 37.0 25.0 39.0 76-77 32.32435586321939 35.0 33.0 36.5 24.5 39.0 78-79 32.022633421482816 35.0 33.0 36.0 24.0 38.0 80-81 31.744817144476468 35.0 33.0 36.0 23.5 37.0 82-83 31.50934541274129 35.0 33.0 35.0 23.0 37.0 84-85 31.28217342719995 35.0 33.0 35.0 21.0 36.0 86-87 31.102321874725433 35.0 33.0 35.0 20.0 36.0 88-89 30.94710783482676 35.0 33.0 35.0 20.0 36.0 90-91 30.80821083360042 35.0 32.5 35.0 19.0 35.5 92-93 30.676471388954543 35.0 32.0 35.0 18.0 35.0 94-95 30.536967096771797 35.0 32.0 35.0 17.0 35.0 96-97 30.425893178665433 35.0 32.0 35.0 15.0 35.0 98-99 30.32696353284321 35.0 32.0 35.0 11.0 35.0 100-101 30.209509192584555 35.0 32.0 35.0 7.5 35.0 102-103 29.364699411085553 34.0 30.0 35.0 7.0 35.0 104-105 29.92949206592773 34.5 31.0 35.0 3.5 35.0 106-107 29.97503703144548 35.0 32.0 35.0 2.0 35.0 108-109 29.88369713041875 35.0 31.5 35.0 2.0 35.0 110-111 29.77899837519506 35.0 31.0 35.0 2.0 35.0 112-113 29.659725255755188 35.0 31.0 35.0 2.0 35.0 114-115 29.507858895652376 34.5 31.0 35.0 2.0 35.0 116-117 29.355398238082806 34.0 31.0 35.0 2.0 35.0 118-119 29.330963980809237 34.5 31.0 35.0 2.0 35.0 120-121 29.467296624043897 35.0 32.0 35.0 2.0 35.0 122-123 29.294897456617214 35.0 32.0 35.0 2.0 35.0 124-125 28.698114830274086 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40197.0 3 6097.0 4 4055.0 5 3664.0 6 3990.0 7 4749.0 8 4738.0 9 4905.0 10 5750.0 11 6209.0 12 6265.0 13 5877.0 14 6222.0 15 5763.0 16 4803.0 17 4481.0 18 4302.0 19 3998.0 20 4179.0 21 4447.0 22 5150.0 23 6737.0 24 9756.0 25 12636.0 26 13164.0 27 14514.0 28 17253.0 29 21129.0 30 26061.0 31 32593.0 32 41937.0 33 56660.0 34 89669.0 35 144219.0 36 309988.0 37 536359.0 38 143618.0 39 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.19517830876324 17.152282595983017 11.49778866069014 25.1547504345636 2 16.849802569657545 19.306094691069937 38.836259361774935 25.00784337749758 3 20.545002933117495 23.57063120193462 28.223273359124967 27.66109250582292 4 13.770894338324293 14.739156478884743 34.60229902697297 36.887650155817994 5 14.434081818541841 38.51066541919132 32.51059363985584 14.544659122410994 6 34.47111692050802 33.363659375419694 17.194246964146572 14.970976739925721 7 30.31603087351401 32.442746438302166 18.97323845785894 18.26798423032488 8 28.198705338606366 35.03543580188624 18.588867347639663 18.17699151186773 9 28.73559235403934 15.84096966156767 17.859076561706214 37.56436142268677 10-11 27.12653194577998 25.45579370353732 26.00892157699919 21.408752773683513 12-13 26.409512533108266 22.75621024571816 26.508052257921 24.32622496325257 14-15 24.21940502701863 24.194468559321876 23.661242727121408 27.92488368653808 16-17 24.525881125243988 27.233894313543562 24.4187424336973 23.821482127515154 18-19 24.425635946980208 26.935608173559594 25.712148311365507 22.926607568094692 20-21 25.27945850438381 25.66522896090721 25.688865870746326 23.366446663962652 22-23 25.30905085799764 25.00727824554836 25.55730013442711 24.126370762026895 24-25 24.731315198875325 25.15071656499267 25.532373045696684 24.58559519043532 26-27 24.182197089465014 25.668804903568958 25.711313886453368 24.437684120512657 28-29 24.20027102115574 26.448601889722852 25.331474961481582 24.019652127639826 30-31 25.29448635898718 25.61918398335766 25.387981949349026 23.698347708306134 32-33 24.828798804020344 25.564467375088253 25.429480329265676 24.17725349162573 34-35 24.67687323183696 25.922786191806864 25.667787070474056 23.73255350588212 36-37 24.5769038591541 25.91024338025206 25.810280042757455 23.702572717836386 38-39 24.6068997778906 26.220727673581173 25.471359829911044 23.701012718617186 40-41 24.681608472784966 25.739654673125045 25.560549770681373 24.01818708340862 42-43 24.27766445588423 25.781598786457106 25.352261269375028 24.58847548828364 44-45 23.932910935371503 25.883389575026893 25.829246567565367 24.354452922036234 46-47 23.923227216684946 25.55270742094622 25.804151126113233 24.7199142362556 48-49 23.951311771055675 26.01884747554033 25.765332562743705 24.26450819066029 50-51 24.112291618132016 25.91550655322166 25.906317842156508 24.065883986489812 52-53 24.284660616377117 25.840020642018757 25.639293097484657 24.23602564411947 54-55 23.665443047569546 25.556437303830343 26.13136811752931 24.646751531070798 56-57 24.00674714272057 25.720294921419985 25.543850703997883 24.729107231861562 58-59 24.04981636295627 25.95007535136383 25.810912783310147 24.189195502369753 60-61 24.750807335350924 25.77656719585625 25.834607476305894 23.63801799248694 62-63 24.41414341107216 25.782237852283547 25.812186188310065 23.99143254833423 64-65 24.074455068163854 25.362367489252957 25.887475887800736 24.675701554782453 66-67 24.148874524754824 25.702422346585802 25.572421192601258 24.57628193605812 68-69 23.690423484372452 25.79932176415572 25.887960834215857 24.62229391725597 70-71 23.78760189106601 25.495420992986002 26.07879152949446 24.638185586453528 72-73 23.762799875504985 26.125482557394864 25.54220609864683 24.569511468453324 74-75 23.697341252628952 25.952626540943 25.750415961023105 24.59961624540494 76-77 23.70189737446539 25.35551020711231 25.85253148265484 25.090060935767468 78-79 23.506153833778733 25.575984115306344 26.04763056825895 24.87023148265597 80-81 23.888846779146085 25.862707457085442 25.61322527801454 24.63522048575393 82-83 24.002841378439804 25.76320278791696 25.909912463020934 24.3240433706223 84-85 23.947211281724435 25.917831275350927 25.46057862771609 24.67437881520855 86-87 23.46485901995393 26.249746689169434 25.656991610964063 24.62840267991257 88-89 23.345526074552996 25.720372582451052 25.926235307839647 25.007866035156308 90-91 23.90681882941278 25.325617737043743 26.05690602897494 24.710657404568533 92-93 24.099698994699846 25.319777524009417 25.89456182520159 24.685961656089148 94-95 23.975154972037586 25.444527497713626 25.740580088583737 24.839737441665047 96-97 23.593166745819712 25.295663702165903 25.999687513052518 25.111482038961864 98-99 23.32010600835716 25.32549057947564 25.841640889574215 25.512762522592986 100-101 23.343291580063127 25.659852445514797 25.949751281031087 25.047104693390988 102-103 23.23670990913729 25.546532914181157 26.05215765294584 25.164599523735713 104-105 23.417229639841548 25.58111330703994 25.795267931505172 25.20638912161334 106-107 23.480772294902675 25.369308996855068 26.087027815548925 25.062890892693336 108-109 23.44833432543243 25.347162493456477 26.007733422766634 25.196769758344463 110-111 23.128405406015823 26.075335601979717 25.903965036568234 24.89229395543623 112-113 23.05610874931665 26.064516847598927 25.9802718918546 24.899102511229827 114-115 23.13471923373805 26.207099246205896 25.769765632371843 24.888415887684214 116-117 22.939814485385977 26.054185463876255 25.83183646597816 25.17416358475961 118-119 22.92653536623898 25.77147778032476 26.04881327790547 25.253173575530784 120-121 22.899550570001985 25.875299418890663 26.193452294394902 25.031697716712447 122-123 22.871203475139147 26.03489555989161 25.908608988374027 25.18529197659522 124-125 22.691850890732134 25.86957873565939 25.80160942177821 25.636960951830268 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 450.0 1 382.5 2 288.0 3 250.0 4 215.5 5 185.5 6 192.5 7 226.5 8 290.0 9 325.5 10 315.5 11 327.0 12 342.5 13 370.0 14 435.5 15 475.5 16 503.0 17 570.5 18 612.0 19 633.5 20 685.0 21 778.0 22 864.5 23 1017.0 24 1209.5 25 1387.5 26 1888.0 27 2685.5 28 3517.5 29 4689.5 30 6308.5 31 8278.5 32 10938.5 33 13947.0 34 17022.5 35 20079.5 36 24050.5 37 28312.5 38 31093.0 39 34171.0 40 37563.0 41 40264.5 42 43586.0 43 47682.5 44 51764.5 45 55876.5 46 60376.0 47 65239.0 48 70163.0 49 74681.0 50 77693.0 51 78713.5 52 77727.5 53 75910.0 54 73766.5 55 70678.0 56 65713.0 57 58861.0 58 52512.0 59 45903.5 60 38261.0 61 31633.0 62 26102.5 63 20057.0 64 14489.5 65 10713.0 66 7976.0 67 5971.5 68 4509.5 69 3305.5 70 2446.5 71 1855.0 72 1303.0 73 893.0 74 635.5 75 419.0 76 278.0 77 178.5 78 102.0 79 68.0 80 47.5 81 39.0 82 36.0 83 19.0 84 13.0 85 18.0 86 14.5 87 8.5 88 6.5 89 6.0 90 3.5 91 1.5 92 4.0 93 3.5 94 1.5 95 2.0 96 2.5 97 1.0 98 0.5 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01268412084192863 2 0.011199150597019912 3 0.010271044193951964 4 0.01274599460213316 5 0.008043588826588887 6 0.026048853046107087 7 0.011199150597019912 8 0.010704160515383672 9 0.0077342200255662375 10-11 0.00692986114290735 12-13 0.005847070339328075 14-15 0.005383017137794101 16-17 0.003990857533192178 18-19 0.004980837696464657 20-21 0.004795216415851068 22-23 0.004733342655646538 24-25 0.005011774576566922 26-27 0.004021794413294443 28-29 0.005197395857180511 30-31 0.004640532015339743 32-33 0.006991734903111879 34-35 0.00680611362249829 36-37 0.0062801866607597855 38-39 0.0060945653801461955 40-41 0.00631112354086205 42-43 0.0061564391403507255 44-45 0.0064039341811688456 46-47 0.007827030665873033 48-49 0.007146419303623204 50-51 0.004980837696464657 52-53 0.004516784494930683 54-55 0.005352080257691836 56-57 0.006342060420964315 58-59 0.0060945653801461955 60-61 0.004640532015339743 62-63 0.004795216415851068 64-65 0.00318649865053329 66-67 0.0033721199311468795 68-69 0.005537701538305426 70-71 0.0042383525740102975 72-73 0.004021794413294443 74-75 0.0035268043316582045 76-77 0.004083668173498973 78-79 0.0029080667296129056 80-81 0.0028771298495106406 82-83 0.004764279535748803 84-85 0.007981715066384357 86-87 0.0059708178597371354 88-89 0.0042383525740102975 90-91 0.004547721375032948 92-93 0.006775176742396025 94-95 0.00593988097963487 96-97 0.007827030665873033 98-99 0.005878007219430341 100-101 0.006991734903111879 102-103 0.004609595135237478 104-105 0.004454910734726153 106-107 0.0062801866607597855 108-109 0.005692385938816751 110-111 0.006527681701577905 112-113 0.004547721375032948 114-115 0.0022583922474653413 116-117 0.002536824168385726 118-119 0.1289449162662403 120-121 0.009095442750065895 122-123 0.0017634021658291023 124-125 0.0014540333648064528 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1616194.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.387164387049594 #Duplication Level Percentage of deduplicated Percentage of total 1 80.08218328431094 48.3593596646352 2 10.689200065559742 12.90980963050035 3 3.523846188908452 6.383852372528787 4 1.7092740992295228 4.128728640507973 5 0.9749476706593947 2.943716262843998 6 0.612059299788442 2.217631533056827 7 0.413218268038879 1.7467155635856997 8 0.3384994880630078 1.6352819384474384 9 0.24724138572557985 1.3437185582783275 >10 1.3339099710044928 14.181179171625475 >50 0.05993998948915065 2.4399415113219924 >100 0.015167929734460328 1.4742409557175185 >500 4.098875903626531E-4 0.16633309929224474 >1k 1.0247189759066327E-4 0.06949109765818112 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.18737602045299E-5 2 0.0 0.0 0.0 0.0 6.18737602045299E-5 3 0.0 0.0 0.0 0.0 6.18737602045299E-5 4 0.0 0.0 0.0 0.0 6.18737602045299E-5 5 0.0 0.0 0.0 0.0 6.18737602045299E-5 6 0.0 0.0 0.0 0.0 6.18737602045299E-5 7 0.0 0.0 0.0 0.0 6.18737602045299E-5 8 0.0 0.0 0.0 0.0 1.237475204090598E-4 9 0.0 0.0 0.0 1.237475204090598E-4 2.474950408181196E-4 10-11 0.0 0.0 0.0 1.237475204090598E-4 2.474950408181196E-4 12-13 0.0 0.0 0.0 1.856212806135897E-4 3.093688010226495E-4 14-15 0.0 0.0 0.0 1.856212806135897E-4 3.4030568112491445E-4 16-17 0.0 0.0 0.0 2.474950408181196E-4 3.712425612271794E-4 18-19 0.0 0.0 0.0 2.474950408181196E-4 3.712425612271794E-4 20-21 0.0 0.0 0.0 2.7843192092038454E-4 3.712425612271794E-4 22-23 0.0 0.0 0.0 4.949900816362392E-4 4.3311632143170926E-4 24-25 0.0 0.0 0.0 9.590432831702135E-4 4.949900816362392E-4 26-27 0.0 0.0 0.0 0.0014540333648064528 5.259269617385041E-4 28-29 0.0 0.0 0.0 0.005908944099532605 5.568638418407691E-4 30-31 0.0 0.0 0.0 0.02249111183434662 5.568638418407691E-4 32-33 0.0 0.0 0.0 0.05305674937538439 5.878007219430341E-4 34-35 0.0 0.0 0.0 0.09386249423027186 6.806113622498289E-4 36-37 0.0 0.0 0.0 0.14905388833271255 6.806113622498289E-4 38-39 0.0 0.0 0.0 0.2417717179992006 6.806113622498289E-4 40-41 0.0 0.0 0.0 0.3536085395688884 6.806113622498289E-4 42-43 0.0 0.0 0.0 0.45761833047270317 6.806113622498289E-4 44-45 0.0 0.0 0.0 0.5756734649429462 6.806113622498289E-4 46-47 0.0 0.0 0.0 0.6911608383647012 6.806113622498289E-4 48-49 0.0 0.0 0.0 0.8150630431742724 6.806113622498289E-4 50-51 0.0 0.0 0.0 0.9427704842364222 7.424851224543588E-4 52-53 0.0 0.0 0.0 1.0784905772450584 7.734220025566237E-4 54-55 0.0 0.0 0.0 1.2315353231109631 8.043588826588887E-4 56-57 0.0 0.0 0.0 1.3888802953110826 8.043588826588887E-4 58-59 0.0 0.0 0.0 1.5656226913353222 8.043588826588887E-4 60-61 0.0 0.0 0.0 1.736518017020234 8.043588826588887E-4 62-63 0.0 0.0 0.0 1.915766300332757 8.043588826588887E-4 64-65 0.0 0.0 0.0 2.098634198617245 8.043588826588887E-4 66-67 0.0 6.18737602045299E-5 0.0 2.3137383259682935 8.043588826588887E-4 68-69 0.0 6.18737602045299E-5 0.0 2.550343584990416 8.043588826588887E-4 70-71 0.0 9.281064030679484E-5 0.0 2.801736672701421 8.352957627611537E-4 72-73 0.0 1.237475204090598E-4 0.0 3.035526675634237 8.662326428634186E-4 74-75 0.0 1.237475204090598E-4 0.0 3.279835217801823 8.662326428634186E-4 76-77 0.0 1.856212806135897E-4 0.0 3.5621651856150933 8.662326428634186E-4 78-79 0.0 1.856212806135897E-4 0.0 3.8801344393061727 8.662326428634186E-4 80-81 0.0 1.856212806135897E-4 0.0 4.18278993734663 8.662326428634186E-4 82-83 0.0 1.856212806135897E-4 0.0 4.486837594991691 9.281064030679485E-4 84-85 0.0 1.856212806135897E-4 0.0 4.802084403233771 9.281064030679485E-4 86-87 0.0 1.856212806135897E-4 0.0 5.163860279149656 9.899801632724785E-4 88-89 0.0 1.856212806135897E-4 0.0 5.576156080272542 0.0011137276836815382 90-91 0.0 1.856212806135897E-4 0.0 6.000176958954185 0.0011137276836815382 92-93 0.0 1.856212806135897E-4 0.0 6.41507145800566 0.0011756014438860682 94-95 0.0 1.856212806135897E-4 0.0 6.836338954358202 0.001237475204090598 96-97 0.0 1.856212806135897E-4 0.0 7.296710667160006 0.001237475204090598 98-99 0.0 1.856212806135897E-4 0.0 7.818492086964808 0.001237475204090598 100-101 0.0 1.856212806135897E-4 0.0 8.386214773721472 0.001237475204090598 102-103 0.0 1.856212806135897E-4 0.0 8.951740941990874 0.001268412084192863 104-105 0.0 2.474950408181196E-4 0.0 9.53313772975274 0.001299348964295128 106-107 0.0 2.474950408181196E-4 0.0 10.161249206469025 0.001299348964295128 108-109 0.0 2.474950408181196E-4 0.0 10.819214772483996 0.0013921596046019228 110-111 0.0 2.474950408181196E-4 0.0 11.514397405261992 0.0014540333648064528 112-113 0.0 2.474950408181196E-4 0.0 12.198473698083275 0.0014849702449087176 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1640 0.0 58.784725 1 GTATCAA 2825 0.0 52.242813 1 GTACATG 9470 0.0 42.22918 1 TCAACGC 3520 0.0 41.41935 4 TACATGG 9710 0.0 40.693844 2 CAACGCA 3605 0.0 40.607822 5 ACATGGG 9920 0.0 39.89237 3 AACGCAG 3835 0.0 38.635536 6 ATCAACG 3880 0.0 37.72969 3 TATCAAC 3915 0.0 37.392387 2 CATGGGG 8550 0.0 37.37559 4 ATGGGGG 5765 0.0 36.954185 5 TGGGGGG 4475 0.0 31.780289 6 ACGCAGA 4905 0.0 30.199923 7 CGCAGAG 5170 0.0 28.536892 8 GTGGTAT 1005 0.0 27.238619 1 GAGTACA 6530 0.0 26.155441 1 AGTACAT 6395 0.0 25.96232 2 TGGTATC 1035 0.0 24.723387 2 GCAGAGT 5970 0.0 24.513554 9 >>END_MODULE