FastQCFastQC Report
Mon 15 Aug 2016
SRR2921322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616194
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27150.16798725895529867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.12232442392435562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17240.10667036259260955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.066.792461
GTATCAA38200.048.0070421
GTACATG89950.044.0957571
TACATGG93000.042.4091642
ACATGGG95900.041.002473
CATGGGG83850.040.1448674
ATGGGGG57100.038.8142745
TCAACGC48600.035.7957344
CAACGCA50250.034.7389145
TATCAAC53550.033.3769342
AACGCAG52700.033.2349056
ATCAACG52350.033.231573
TGGGGGG42650.032.4060976
GTGGTAT10450.030.867921
TGGTATC11050.027.497462
ACGCAGA64900.026.247327
CGCAGAG68200.025.147288
GAGTACA61050.023.4831051
AGTACAT60000.022.6395762
ATGGGGA25550.022.3854085
Produced by FastQC (version 0.11.5)