##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921322_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1616194
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.30430505248751	33.0	30.0	34.0	19.0	34.0
2	30.047019107854627	33.0	31.0	34.0	26.0	34.0
3	30.284253623018028	33.0	31.0	34.0	27.0	34.0
4	32.46001841363104	37.0	35.0	37.0	19.0	37.0
5	32.13073863657457	37.0	33.0	37.0	19.0	37.0
6	32.29369927125085	37.0	35.0	37.0	17.0	37.0
7	32.504750048570905	37.0	35.0	37.0	17.0	37.0
8	32.75897138586085	37.0	35.0	37.0	17.0	37.0
9	34.51311909337617	39.0	37.0	39.0	17.0	39.0
10-11	34.694729407484495	39.0	37.0	39.0	25.5	39.0
12-13	34.71134869947544	39.0	37.0	39.0	25.5	39.0
14-15	36.07258379872714	40.5	38.0	41.0	26.0	41.0
16-17	36.11714002155682	41.0	38.0	41.0	27.0	41.0
18-19	36.14977904880231	41.0	38.0	41.0	27.0	41.0
20-21	36.149874334393026	41.0	38.0	41.0	27.0	41.0
22-23	36.09838237241321	41.0	38.0	41.0	26.5	41.0
24-25	36.03211588460296	41.0	38.0	41.0	25.5	41.0
26-27	35.93558230014466	40.5	38.0	41.0	25.0	41.0
28-29	35.8707636583232	40.0	37.5	41.0	24.5	41.0
30-31	35.773777776677804	40.0	37.0	41.0	24.0	41.0
32-33	35.64034237226471	40.0	37.0	41.0	24.0	41.0
34-35	35.55964382988676	40.0	37.0	41.0	23.0	41.0
36-37	35.481192542479434	40.0	36.0	41.0	22.5	41.0
38-39	35.38603533981688	40.0	36.0	41.0	21.5	41.0
40-41	35.252181978153615	40.0	36.0	41.0	18.5	41.0
42-43	35.10263495595207	40.0	35.0	41.0	17.0	41.0
44-45	34.94559223707055	40.0	35.0	41.0	14.5	41.0
46-47	34.727164870058914	40.0	35.0	41.0	8.5	41.0
48-49	34.50004052731293	40.0	35.0	41.0	4.5	41.0
50-51	34.308808534124	40.0	35.0	41.0	2.0	41.0
52-53	34.12880013166736	39.0	35.0	41.0	2.0	41.0
54-55	33.934035146770746	39.0	35.0	41.0	2.0	41.0
56-57	33.69502949522149	39.0	35.0	41.0	2.0	41.0
58-59	33.637453795769574	39.0	35.0	41.0	2.0	41.0
60-61	33.39195603993085	38.0	34.5	41.0	2.0	41.0
62-63	33.103026616854166	37.0	34.0	40.0	2.0	41.0
64-65	32.80729448321179	37.0	34.0	40.0	2.0	41.0
66-67	32.49369815752317	36.0	34.0	39.0	2.0	41.0
68-69	32.15440937164722	36.0	34.0	39.0	2.0	41.0
70-71	31.82355738234395	35.0	33.5	38.5	2.0	40.5
72-73	31.481240494643586	35.0	33.0	37.0	2.0	39.5
74-75	31.142223025206135	35.0	33.0	37.0	2.0	39.0
76-77	30.846029313312634	35.0	33.0	36.0	2.0	39.0
78-79	30.585246573121793	35.0	33.0	36.0	2.0	37.5
80-81	30.359306494146125	35.0	33.0	35.5	2.0	37.0
82-83	30.16047887815448	35.0	32.5	35.0	2.0	37.0
84-85	29.98873681006117	35.0	32.0	35.0	2.0	36.0
86-87	29.848543862927347	35.0	32.0	35.0	2.0	36.0
88-89	29.72660027199705	35.0	32.0	35.0	2.0	36.0
90-91	29.629241910315223	35.0	32.0	35.0	2.0	35.0
92-93	29.55750547273409	35.0	32.0	35.0	2.0	35.0
94-95	29.482512619153393	35.0	32.0	35.0	2.0	35.0
96-97	29.41823537273372	35.0	32.0	35.0	2.0	35.0
98-99	29.366660190546433	35.0	32.0	35.0	2.0	35.0
100-101	29.31476264606848	35.0	32.0	35.0	2.0	35.0
102-103	28.630641494771048	34.0	30.0	35.0	2.0	35.0
104-105	29.17811537476318	35.0	31.0	35.0	2.0	35.0
106-107	29.232161794933035	35.0	32.0	35.0	2.0	35.0
108-109	29.29925893797403	35.0	32.0	35.0	2.0	35.0
110-111	29.40541791393855	35.0	32.0	35.0	2.0	35.0
112-113	29.360200569981078	35.0	32.0	35.0	2.0	35.0
114-115	29.290741086775476	35.0	32.0	35.0	2.0	35.0
116-117	29.217164214197055	35.0	32.0	35.0	2.0	35.0
118-119	29.1292657317129	35.0	32.0	35.0	2.0	35.0
120-121	29.02240851036447	35.0	32.0	35.0	2.0	35.0
122-123	28.89962188945139	35.0	32.0	35.0	2.0	35.0
124-125	28.36831809795111	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107280.0
3	6131.0
4	4664.0
5	4540.0
6	4704.0
7	4910.0
8	5401.0
9	5188.0
10	5464.0
11	5604.0
12	6284.0
13	7895.0
14	9555.0
15	8302.0
16	5478.0
17	4663.0
18	4144.0
19	3759.0
20	3591.0
21	3718.0
22	4172.0
23	5223.0
24	6690.0
25	8422.0
26	10101.0
27	11809.0
28	13882.0
29	16741.0
30	20621.0
31	26113.0
32	33137.0
33	44818.0
34	70563.0
35	119184.0
36	259136.0
37	558268.0
38	195756.0
39	283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.31048434137466	17.264016064456396	11.346080813747124	27.079418780421815
2	16.132221750606675	19.390184594176194	37.841806119809874	26.635787535407257
3	19.481139021676853	23.155202902621838	28.529495840227103	28.83416223547421
4	13.639635012330245	15.532706868117419	31.47749459294716	39.35016352660517
5	14.207110836794309	38.12553216013326	32.03030787516437	15.637049127908057
6	34.37863647392633	33.518138422457376	16.40783225301688	15.695392850599415
7	30.115906099288498	32.53669679065919	18.224406931963262	19.12299017808905
8	29.12248829111907	34.54783516420278	17.58408149096487	18.745595053713274
9	28.736357639907283	15.536233390631555	17.746793836542903	37.98061513291826
10-11	27.338067176350915	25.555884180070837	25.043343373568522	22.062705270009726
12-13	26.910431669040424	22.745948643243178	25.94926854092961	24.39435114678679
14-15	24.728683561503136	24.16420924715721	23.39889270718738	27.708214484152272
16-17	24.53081127636905	27.074998422219114	24.14973078726935	24.244459514142484
18-19	24.644411500104564	26.16752691817938	25.95734175476459	23.230719826951468
20-21	25.18880777926412	25.052438011773337	26.179282932618236	23.5794712763443
22-23	25.171575937047162	24.384943886686873	26.014544046073677	24.42893613019229
24-25	24.67055316379098	24.812770001621093	25.935036264210854	24.581640570377072
26-27	24.154526003685202	25.065493375176494	26.261358475529544	24.518622145608756
28-29	24.09630279533274	25.76135661931674	25.924332103695473	24.218008481655048
30-31	25.034247126273208	25.144258671916862	25.983019365249472	23.838474836560465
32-33	24.595933408984315	25.2431329407237	26.003375832356756	24.157557817935224
34-35	24.689362786893156	25.456721160949737	26.251984600858556	23.601931451298544
36-37	24.62204413579063	25.298231524185834	26.473709220551495	23.606015119472044
38-39	24.37674561345977	25.784497405633232	26.225564505251224	23.61319247565577
40-41	24.36115342588823	25.652675359517485	26.025866944191105	23.96030427040318
42-43	24.12606407399112	25.571001996047503	25.788581073806732	24.514352856154645
44-45	23.796307868981074	25.696667603022906	26.30086487142014	24.20615965657588
46-47	23.79689566970302	25.474850172689667	25.89556080520037	24.832693352406952
48-49	23.971286862839484	25.920588741203098	25.73388466978593	24.37423972617149
50-51	24.096117174052125	25.88838344901664	25.899737284014172	24.115762092917063
52-53	24.32220389383948	25.734441533627773	25.68197258497433	24.261381987558426
54-55	23.593207251109703	25.489328632577525	26.210405434001117	24.707058682311654
56-57	23.839341069203325	25.6050635010401	25.680889794170746	24.87470563558583
58-59	23.947898581482175	25.738865507482394	25.91981531920054	24.39342059183489
60-61	24.58541486974955	25.57821028911133	26.05228703979844	23.784087801340682
62-63	24.17163409838175	25.580747113279717	26.002664284114406	24.244954504224122
64-65	23.83528833790993	25.166997278792024	26.076850922599636	24.920863460698406
66-67	23.912259295604365	25.593554981642054	25.70279310528315	24.79139261747043
68-69	23.53643807612205	25.5600194036112	25.985556189417853	24.917986330848898
70-71	23.676520269225104	25.34299718969381	26.138446250883245	24.842036290197836
72-73	23.70157914210794	25.878669268664527	25.63272107185152	24.787030517376007
74-75	23.595465643357173	25.797336210875677	25.783631172990372	24.823566972776785
76-77	23.554288655941054	25.21293854574389	25.831645210909087	25.40112758740597
78-79	23.30914481801071	25.44830632956192	26.036756725987104	25.20579212644027
80-81	23.711571754380973	25.628420845517308	25.640053112435762	25.019954287665964
82-83	23.708942119572278	25.632349829290295	25.863726755575133	24.79498129556229
84-85	23.583090891316267	25.738030211719632	25.44023180385523	25.238647093108874
86-87	23.147004629394736	26.06781735360978	25.569702647083208	25.215475369912276
88-89	23.037395263192415	25.59154408443541	25.888878439098278	25.4821822132739
90-91	23.50068045773041	25.22884819032893	25.990855662834512	25.27961568910615
92-93	23.614410195837245	25.249769674215084	25.836984412095365	25.29883571785231
94-95	23.558896467746408	25.271378648081416	25.59581411119471	25.57391077297747
96-97	23.118837932580266	25.18360327399929	25.829041600036135	25.86851719338431
98-99	22.86474360897999	25.225290164822468	25.778318004620115	26.13164822157743
100-101	22.913059942061412	25.56073095185355	25.880401733950254	25.645807372134783
102-103	22.77319430711907	25.342471262732076	25.919505950399518	25.964828479749336
104-105	22.93709789790087	25.35759939710208	25.685592199946296	26.019710505050753
106-107	23.054934067239536	25.163841919771908	25.8472384469172	25.93398556607136
108-109	22.929317960126557	25.164607964974383	25.86452632226996	26.0415477526291
110-111	22.67416661226917	25.797067732465777	25.788405381918395	25.740360273346656
112-113	22.6362810624051	25.759320423071163	25.76813749936579	25.836261015157945
114-115	22.537033061067657	25.875592674380687	25.614918038771407	25.972456225780245
116-117	22.34701545522804	25.575978485129635	25.79154793280099	26.285458126841338
118-119	22.35029770614983	25.304249312117193	25.9714043699043	26.374048611828677
120-121	22.40566096107393	25.423155169682808	25.96690244723399	26.204281422009267
122-123	22.301043859033086	25.59272203699309	25.69493922810552	26.411294875868297
124-125	21.961172905925107	25.482198576777126	25.5166623251831	27.03996619211467
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	85.0
1	78.5
2	84.0
3	91.0
4	81.5
5	104.0
6	228.0
7	548.5
8	794.5
9	923.5
10	939.0
11	787.5
12	744.0
13	829.5
14	929.5
15	956.0
16	980.0
17	1070.5
18	1171.5
19	1225.0
20	1274.0
21	1359.5
22	1445.5
23	1547.0
24	1704.0
25	1960.5
26	2502.0
27	3175.5
28	3781.5
29	4750.0
30	6230.0
31	7940.0
32	10302.0
33	12947.0
34	15660.5
35	18569.0
36	22040.5
37	26074.0
38	28986.5
39	32095.0
40	35365.5
41	37923.0
42	41329.0
43	45725.0
44	49872.0
45	53820.0
46	58446.0
47	63126.5
48	68456.0
49	73805.0
50	77457.0
51	79434.0
52	78698.5
53	76601.5
54	74951.0
55	72253.5
56	67138.5
57	60079.0
58	53866.5
59	47420.5
60	39675.0
61	33026.5
62	27286.5
63	21092.0
64	15299.0
65	11319.5
66	8597.5
67	6522.5
68	4888.5
69	3605.5
70	2764.0
71	2164.5
72	1554.5
73	1100.5
74	838.5
75	624.5
76	448.0
77	311.5
78	237.0
79	195.5
80	196.5
81	184.0
82	158.0
83	152.5
84	139.5
85	107.5
86	92.5
87	104.0
88	99.0
89	93.0
90	99.0
91	83.5
92	67.5
93	70.0
94	72.5
95	65.0
96	49.5
97	43.0
98	31.5
99	12.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2899404403184271
2	0.0
3	0.0
4	0.015406566290927947
5	0.1539419153888704
6	0.03823798380639948
7	0.01961398198483598
8	0.006991734903111879
9	0.005444890897998632
10-11	0.001856212806135897
12-13	0.05961536795706456
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.5468440051132475E-4
92-93	3.093688010226495E-4
94-95	1.2374752040905982E-4
96-97	3.403056811249145E-4
98-99	3.0936880102264955E-5
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.2374752040905982E-4
108-109	3.403056811249145E-4
110-111	2.7843192092038454E-4
112-113	7.424851224543588E-4
114-115	1.856212806135897E-4
116-117	5.878007219430341E-4
118-119	1.856212806135897E-4
120-121	1.2374752040905982E-4
122-123	0.0017015284056245725
124-125	1.856212806135897E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1616194.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.95486589188501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.48105578681721	51.92583805680163
2	9.47962411876795	11.935769302050298
3	3.032076429856989	5.726518950467767
4	1.4575438403293868	3.670379079979184
5	0.8513837469831698	2.6799374806928
6	0.5407761250521668	2.0426693058115433
7	0.38208939287622457	1.6838070541064134
8	0.2903373522830463	1.462251926110732
9	0.2215790316686481	1.2554530400838135
>10	1.1952144233883493	13.232652222762779
>50	0.05161591209568299	2.2013893983033928
>100	0.016016124742764606	1.5796779833247188
>500	2.947350593072208E-4	0.1413891467283002
>1k	3.929800790762944E-4	0.46226705277660357
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2715	0.16798725895529867	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1977	0.12232442392435562	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1724	0.10667036259260955	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.18737602045299E-5	0.0
5	0.0	0.0	0.0	6.18737602045299E-5	0.0
6	0.0	0.0	0.0	6.18737602045299E-5	0.0
7	0.0	0.0	0.0	6.18737602045299E-5	0.0
8	0.0	0.0	0.0	6.18737602045299E-5	0.0
9	0.0	0.0	0.0	1.237475204090598E-4	0.0
10-11	0.0	0.0	0.0	1.237475204090598E-4	0.0
12-13	0.0	0.0	0.0	1.237475204090598E-4	3.093688010226495E-5
14-15	0.0	0.0	0.0	1.237475204090598E-4	1.5468440051132475E-4
16-17	0.0	0.0	0.0	1.856212806135897E-4	2.474950408181196E-4
18-19	0.0	0.0	0.0	1.856212806135897E-4	2.474950408181196E-4
20-21	0.0	0.0	0.0	2.1655816071585463E-4	2.474950408181196E-4
22-23	0.0	0.0	0.0	4.3311632143170926E-4	3.093688010226495E-4
24-25	0.0	0.0	0.0	8.971695229656835E-4	3.712425612271794E-4
26-27	0.0	0.0	0.0	0.0013921596046019228	3.712425612271794E-4
28-29	0.0	0.0	0.0	0.005723322818919015	3.712425612271794E-4
30-31	0.0	0.0	0.0	0.021655816071585468	3.712425612271794E-4
32-33	0.0	0.0	0.0	0.05002493512536243	3.712425612271794E-4
34-35	0.0	0.0	0.0	0.08838666645217097	4.949900816362392E-4
36-37	0.0	0.0	0.0	0.13998938246274892	4.949900816362392E-4
38-39	0.0	0.0	0.0	0.22766450067256777	4.949900816362392E-4
40-41	0.0	0.0	0.0	0.333128324941189	4.949900816362392E-4
42-43	0.0	0.0	0.0	0.42962045398015336	5.259269617385041E-4
44-45	0.0	0.0	0.0	0.5379304712181829	5.568638418407691E-4
46-47	0.0	0.0	0.0	0.6454980033337583	6.18737602045299E-4
48-49	0.0	0.0	0.0	0.7611709980361269	6.806113622498289E-4
50-51	0.0	0.0	0.0	0.879597375067597	6.806113622498289E-4
52-53	0.0	0.0	0.0	1.0062220253261676	6.806113622498289E-4
54-55	0.0	0.0	0.0	1.1506663185236428	6.806113622498289E-4
56-57	0.0	0.0	0.0	1.2997202068563551	6.806113622498289E-4
58-59	0.0	0.0	0.0	1.466315306207052	6.806113622498289E-4
60-61	0.0	6.18737602045299E-5	0.0	1.6273108302592387	7.424851224543588E-4
62-63	0.0	6.18737602045299E-5	0.0	1.797834913382923	7.424851224543588E-4
64-65	0.0	6.18737602045299E-5	0.0	1.9705555149938683	7.424851224543588E-4
66-67	0.0	6.18737602045299E-5	0.0	2.1748007974290218	7.424851224543588E-4
68-69	0.0	6.18737602045299E-5	0.0	2.3993097363311584	7.424851224543588E-4
70-71	0.0	6.18737602045299E-5	0.0	2.6349250151900083	8.662326428634186E-4
72-73	0.0	6.18737602045299E-5	6.18737602045299E-5	2.8539890631941462	8.971695229656835E-4
74-75	0.0	6.18737602045299E-5	6.18737602045299E-5	3.0810967000248732	9.281064030679485E-4
76-77	0.0	6.18737602045299E-5	6.18737602045299E-5	3.343008326970648	9.281064030679485E-4
78-79	0.0	6.18737602045299E-5	6.18737602045299E-5	3.6434673065238457	9.281064030679485E-4
80-81	0.0	6.18737602045299E-5	6.18737602045299E-5	3.927282244582024	9.590432831702134E-4
82-83	0.0	6.18737602045299E-5	6.18737602045299E-5	4.209859707436112	0.001237475204090598
84-85	0.0	6.18737602045299E-5	6.18737602045299E-5	4.5059875237749925	0.001237475204090598
86-87	0.0	6.18737602045299E-5	6.18737602045299E-5	4.84551978289735	0.001237475204090598
88-89	0.0	6.18737602045299E-5	6.18737602045299E-5	5.235850399147627	0.001237475204090598
90-91	0.0	6.18737602045299E-5	6.18737602045299E-5	5.630604989252528	0.001237475204090598
92-93	0.0	6.18737602045299E-5	6.18737602045299E-5	6.0168210004492035	0.001299348964295128
94-95	0.0	6.18737602045299E-5	6.18737602045299E-5	6.4115137167939	0.001299348964295128
96-97	0.0	6.18737602045299E-5	6.18737602045299E-5	6.843640058062336	0.001299348964295128
98-99	0.0	6.18737602045299E-5	6.18737602045299E-5	7.336495494971519	0.001299348964295128
100-101	0.0	6.18737602045299E-5	6.18737602045299E-5	7.870001992335078	0.001299348964295128
102-103	0.0	6.18737602045299E-5	6.18737602045299E-5	8.402394762014955	0.0013612227244996578
104-105	0.0	2.1655816071585463E-4	6.18737602045299E-5	8.949637234143921	0.0013921596046019228
106-107	0.0	2.474950408181196E-4	6.18737602045299E-5	9.545481544913544	0.0014230964847041878
108-109	0.0	2.474950408181196E-4	6.18737602045299E-5	10.17018996481858	0.0015468440051132476
110-111	0.0	2.474950408181196E-4	6.18737602045299E-5	10.827134613790175	0.001794339045931367
112-113	0.0	2.474950408181196E-4	6.18737602045299E-5	11.474952883131603	0.001856212806135897
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2370	0.0	66.79246	1
GTATCAA	3820	0.0	48.007042	1
GTACATG	8995	0.0	44.095757	1
TACATGG	9300	0.0	42.409164	2
ACATGGG	9590	0.0	41.00247	3
CATGGGG	8385	0.0	40.144867	4
ATGGGGG	5710	0.0	38.814274	5
TCAACGC	4860	0.0	35.795734	4
CAACGCA	5025	0.0	34.738914	5
TATCAAC	5355	0.0	33.376934	2
AACGCAG	5270	0.0	33.234905	6
ATCAACG	5235	0.0	33.23157	3
TGGGGGG	4265	0.0	32.406097	6
GTGGTAT	1045	0.0	30.86792	1
TGGTATC	1105	0.0	27.49746	2
ACGCAGA	6490	0.0	26.24732	7
CGCAGAG	6820	0.0	25.14728	8
GAGTACA	6105	0.0	23.483105	1
AGTACAT	6000	0.0	22.639576	2
ATGGGGA	2555	0.0	22.385408	5
>>END_MODULE