FastQCFastQC Report
Mon 15 Aug 2016
SRR2921321_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921321_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585263
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.050.9418561
GTATCAA21050.050.0405651
TCAACGC25000.041.890824
CAACGCA25600.041.3725785
GTACATG74950.040.097821
AACGCAG27200.038.7164766
TACATGG77750.038.5723762
ACATGGG79150.037.2886773
ATCAACG28550.036.4735573
TATCAAC29250.036.2109832
CATGGGG73000.033.9906434
ATGGGGG44800.030.4151525
ACGCAGA36400.029.0935237
CGCAGAG38550.027.4700628
GAGTACA52850.025.8990541
AGTACAT50400.025.3836522
TGGGGGG29900.023.4802236
GCAGAGT47450.022.1915369
AGAGTAC43100.021.3986785
GTGGTAT9500.020.672421
Produced by FastQC (version 0.11.5)