##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921321_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1585263
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.690162452539422	31.0	31.0	34.0	27.0	34.0
2	30.70531829734246	33.0	31.0	34.0	27.0	34.0
3	30.88327804282318	33.0	31.0	34.0	27.0	34.0
4	34.361909033390674	37.0	35.0	37.0	32.0	37.0
5	34.36210773858975	37.0	35.0	37.0	31.0	37.0
6	34.44166866948891	37.0	35.0	37.0	32.0	37.0
7	34.32161855793014	37.0	35.0	37.0	31.0	37.0
8	34.3130477403434	37.0	35.0	37.0	31.0	37.0
9	35.9352978023205	39.0	37.0	39.0	31.0	39.0
10-11	35.98311857401579	39.0	37.0	39.0	31.5	39.0
12-13	35.941562693382735	39.0	37.0	39.0	31.0	39.0
14-15	37.27113387494693	41.0	38.0	41.0	31.0	41.0
16-17	37.252600987974866	41.0	38.0	41.0	31.0	41.0
18-19	37.230765494432156	40.5	38.0	41.0	31.0	41.0
20-21	37.2731291274697	41.0	38.0	41.0	31.0	41.0
22-23	37.210212753341246	40.0	38.0	41.0	31.0	41.0
24-25	37.1876798991713	40.5	38.0	41.0	31.0	41.0
26-27	37.16229862174289	40.0	38.0	41.0	31.0	41.0
28-29	37.10052937588274	40.0	38.0	41.0	30.0	41.0
30-31	37.035086607080345	40.0	38.0	41.0	30.0	41.0
32-33	36.964583163803105	40.0	38.0	41.0	30.0	41.0
34-35	36.853854849321536	40.0	38.0	41.0	30.0	41.0
36-37	36.745617919550256	40.0	38.0	41.0	30.0	41.0
38-39	36.64581271372637	40.0	37.0	41.0	30.0	41.0
40-41	36.54636170780495	40.0	37.0	41.0	30.0	41.0
42-43	36.35000753818136	40.0	37.0	41.0	29.0	41.0
44-45	36.08703603124529	40.0	36.0	41.0	28.5	41.0
46-47	35.86579608557066	40.0	36.0	41.0	28.0	41.0
48-49	35.660540238433626	40.0	35.0	41.0	27.0	41.0
50-51	35.48619976622176	39.0	35.0	41.0	27.0	41.0
52-53	35.28938889004537	39.0	35.0	41.0	26.5	41.0
54-55	35.07985078816575	39.0	35.0	40.5	26.0	41.0
56-57	34.83107850243146	38.5	35.0	40.0	26.0	41.0
58-59	34.5586520974753	38.0	34.0	40.0	26.0	41.0
60-61	34.26669013280446	37.5	34.0	40.0	25.0	41.0
62-63	33.94109400143699	37.0	34.0	40.0	24.5	41.0
64-65	33.59394119461565	36.5	34.0	39.5	23.5	41.0
66-67	33.254904706663815	36.0	33.0	39.0	23.0	41.0
68-69	32.89795573352813	35.5	33.0	39.0	22.0	40.0
70-71	32.859018976661915	35.0	33.0	38.5	23.0	40.0
72-73	32.56715321053983	35.0	33.0	37.0	22.5	39.5
74-75	32.24060644826758	35.0	33.0	37.0	22.0	39.0
76-77	31.94129144501575	35.0	33.0	36.5	21.5	39.0
78-79	31.642789871459815	35.0	33.0	36.0	20.5	37.5
80-81	31.364721185065193	35.0	33.0	35.5	20.0	37.0
82-83	31.1427201669376	35.0	32.5	35.0	19.5	37.0
84-85	30.920525805497256	35.0	32.0	35.0	18.5	36.0
86-87	30.746012491302704	35.0	32.0	35.0	18.0	36.0
88-89	30.59983800795199	35.0	32.0	35.0	17.0	36.0
90-91	30.469405076633972	35.0	32.0	35.0	15.5	35.0
92-93	30.354345619622737	35.0	32.0	35.0	11.5	35.0
94-95	30.226952247040398	35.0	32.0	35.0	9.0	35.0
96-97	30.128573618383825	35.0	31.5	35.0	7.0	35.0
98-99	30.037853971233794	35.0	31.0	35.0	7.0	35.0
100-101	29.936151288461282	35.0	31.0	35.0	3.5	35.0
102-103	29.060774458244467	33.5	29.5	34.5	2.0	35.0
104-105	29.66912114898285	34.0	31.0	35.0	2.0	35.0
106-107	29.75021684099105	34.0	31.0	35.0	2.0	35.0
108-109	29.676231956464008	34.0	31.0	35.0	2.0	35.0
110-111	29.581160034644093	34.0	31.0	35.0	2.0	35.0
112-113	29.476399184236307	34.0	31.0	35.0	2.0	35.0
114-115	29.33936608625824	34.0	31.0	35.0	2.0	35.0
116-117	29.203502510309015	34.0	31.0	35.0	2.0	35.0
118-119	29.202297662911455	34.0	30.5	35.0	2.0	35.0
120-121	29.363282937909986	35.0	31.0	35.0	2.0	35.0
122-123	29.215107209340026	35.0	31.0	35.0	2.0	35.0
124-125	28.613113723085696	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48168.0
3	7150.0
4	4520.0
5	3724.0
6	4078.0
7	4592.0
8	4964.0
9	5240.0
10	5640.0
11	5996.0
12	6018.0
13	5957.0
14	5959.0
15	5376.0
16	4825.0
17	4612.0
18	4458.0
19	4299.0
20	4222.0
21	4462.0
22	5095.0
23	6724.0
24	9245.0
25	11656.0
26	12863.0
27	14486.0
28	17760.0
29	21492.0
30	25950.0
31	32682.0
32	43022.0
33	58102.0
34	90176.0
35	148608.0
36	315507.0
37	503184.0
38	124405.0
39	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73653094301221	17.214311896276417	11.768331652007905	25.280825508703465
2	16.481241163767677	18.870880131323524	38.94093280331185	25.70694590159694
3	20.46793144172244	22.7485388971323	28.169381945916484	28.614147715228782
4	13.661426959969111	14.062164842569064	34.398855323025515	37.877552874436304
5	14.475044712280027	37.76675172777082	32.85190220391323	14.906301356035922
6	35.505075488746094	33.36976109111651	16.359428395479256	14.765735024658142
7	31.177898597811865	31.266536874153832	19.00764244896517	18.547922079069128
8	28.012266718482472	35.68136309209123	18.217061110262936	18.08930907916336
9	27.896883074935403	15.818167595284239	17.794318778262273	38.49063055151809
10-11	27.104961013399276	25.233698806429633	26.088659819828912	21.572680360342172
12-13	26.479332868189864	22.29846691908901	26.751605817405533	24.470594395315594
14-15	23.937090976533863	23.87835940190944	23.659141118536844	28.52540850301985
16-17	24.53666700521512	27.10953009685219	24.35192679278753	24.001876105145158
18-19	24.57830512045789	26.592555232669085	25.58380575587449	23.24533389099853
20-21	25.167472143700216	25.455892010353338	25.54187544829039	23.83476039765605
22-23	25.1093483321994	25.051594875429483	25.45309337418657	24.38596341818455
24-25	24.6224874288645	25.287175770054994	25.463843515464184	24.626493285616323
26-27	24.388316000538733	25.524013314462874	25.566247906012734	24.52142277898566
28-29	24.491855173362236	26.01398707274514	25.37945265452839	24.114705099364237
30-31	25.025273035524187	25.555304971809328	25.336340338288053	24.083081654378425
32-33	24.596176156461276	25.582354830020204	25.41111017885272	24.410358834665796
34-35	24.556384355861503	25.779134671918726	25.582245532931776	24.082235439287995
36-37	24.659050011465673	25.712220135865856	25.496375301900464	24.132354550768014
38-39	24.67467866879347	25.83175174043561	25.474123016332385	24.019446574438536
40-41	24.623957874323846	25.60265941356764	25.519544853947057	24.253837858161457
42-43	24.42682695261761	25.675906702574558	25.35811684194788	24.53914950285995
44-45	24.181024516982916	25.699950131421527	25.703861392478327	24.41516395911723
46-47	24.25437997451961	25.491746630661442	25.605995178345392	24.647878216473558
48-49	24.1776488029524	25.828912090338452	25.567674983440053	24.425764123269094
50-51	24.211131592460678	25.77902726626974	25.61462973967658	24.395211401593002
52-53	24.347135179362176	25.772677221260714	25.520940280528503	24.359247318848603
54-55	24.12450711386762	25.411933104063678	25.831980436272634	24.631579345796073
56-57	24.18741795007832	25.653765574896713	25.553460432775683	24.605356042249284
58-59	24.259114046946593	25.773374725501412	25.624399509703416	24.343111717848576
60-61	24.661333545295573	25.60510172925861	25.65241472935702	24.08114999608879
62-63	24.454676698312845	25.72904020260115	25.644349692007268	24.17193340707874
64-65	24.282902580730628	25.433286441544023	25.679468335425593	24.60434264229976
66-67	24.276168000560176	25.70683108662643	25.46541181635986	24.551589096453526
68-69	24.116619337492498	25.619986947809213	25.640142457502314	24.623251257195975
70-71	24.035835372779413	25.471212189552883	25.831704457531522	24.661247980136178
72-73	24.1433094519994	25.82675739765165	25.435576227338018	24.594356923010928
74-75	23.9535580571598	25.771714068527412	25.625392299418685	24.6493355748941
76-77	24.064452638318944	25.378801977043835	25.672898286961544	24.88384709767568
78-79	23.95153221828165	25.380988608506843	25.946462883969275	24.721016289242236
80-81	24.13685318792317	25.735214800245014	25.462570739526512	24.665361272305304
82-83	24.271125623271494	25.72322834446979	25.59494670650221	24.410699325756504
84-85	24.25632127312133	25.73211825932479	25.39883613060899	24.61272433694489
86-87	23.882524865637397	25.935491441136104	25.64180045099278	24.540183242233716
88-89	23.85599103705353	25.529793533027206	25.75377256901841	24.86044286090085
90-91	24.11325975215558	25.426574008887275	25.7483654935566	24.711800745400545
92-93	24.152962718215697	25.593355865268908	25.685838788890063	24.567842627625332
94-95	24.12883702048777	25.418544625151128	25.707063394957814	24.745554959403293
96-97	23.93613967663567	25.424518987828886	25.761364718855727	24.877976616679714
98-99	23.772501987962773	25.477812884522148	25.603698793596003	25.14598633391908
100-101	23.877879642874603	25.45053094093174	25.776555326521184	24.895034089672478
102-103	23.696313527579544	25.41920119859638	25.931033395103103	24.953451878720973
104-105	23.814882933702332	25.48534356890004	25.696264457638087	25.003509039759546
106-107	23.793555810769167	25.380173477263718	25.903999237933984	24.922271474033135
108-109	23.734397611871	25.37462764340066	25.881385892021946	25.009588852706386
110-111	23.64909876832933	25.725003848182272	25.74989086302742	24.876006520460976
112-113	23.637099999621494	25.75073209074519	25.797414309740308	24.81475359989301
114-115	23.70571253111939	25.794216658765585	25.697637339293365	24.802433470821658
116-117	23.405229745641066	25.672548393109977	25.85078858114691	25.071433280102042
118-119	23.51040855398255	25.537955834152893	25.890571379303157	25.061064232561403
120-121	23.44397574570782	25.561384411226523	26.043050958554282	24.951588884511377
122-123	23.368060155308694	25.80581366741735	25.786478933656316	25.039647243617647
124-125	23.327265908695928	25.66293526885336	25.802503908710438	25.207294913740274
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	283.0
1	250.5
2	201.0
3	161.5
4	139.0
5	123.5
6	117.0
7	145.0
8	189.0
9	212.5
10	213.0
11	211.5
12	224.5
13	254.5
14	282.0
15	309.0
16	331.0
17	373.5
18	433.5
19	474.0
20	505.5
21	536.0
22	634.5
23	796.0
24	985.5
25	1248.0
26	1618.5
27	2236.0
28	3046.5
29	4124.5
30	5715.5
31	7436.5
32	9727.5
33	12464.0
34	15268.5
35	18245.0
36	21892.5
37	26066.5
38	29666.5
39	33330.5
40	36896.5
41	39876.5
42	43544.0
43	48233.0
44	52460.5
45	57003.5
46	60914.5
47	64237.5
48	69112.0
49	72475.5
50	73473.5
51	74753.0
52	74358.5
53	72366.5
54	71640.5
55	69644.5
56	64976.5
57	59438.0
58	54207.0
59	48228.5
60	40842.5
61	33746.5
62	27010.0
63	20390.5
64	14843.5
65	10813.5
66	7959.0
67	5965.0
68	4471.5
69	3228.5
70	2334.5
71	1692.5
72	1188.5
73	797.0
74	553.0
75	389.0
76	256.5
77	154.5
78	109.0
79	79.0
80	47.0
81	36.5
82	35.0
83	33.0
84	21.0
85	14.0
86	16.5
87	14.0
88	7.0
89	4.5
90	6.0
91	5.5
92	4.0
93	4.0
94	2.5
95	1.0
96	2.0
97	2.5
98	3.0
99	2.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012868527178140158
2	0.01072377264845013
3	0.010029881477079828
4	0.011796149913295145
5	0.008074369994127157
6	0.024664677091435303
7	0.010660691632871012
8	0.010156043508238066
9	0.0070019927292821445
10-11	0.0064973446046491965
12-13	0.005614210386541539
14-15	0.005361886324225065
16-17	0.0040371849970635785
18-19	0.00520418378527727
20-21	0.004667995152854763
22-23	0.004825697691802559
24-25	0.0052988053086459475
26-27	0.00438413058274873
28-29	0.005361886324225065
30-31	0.004762616676223441
32-33	0.006560425620228315
34-35	0.0067496686669656705
36-37	0.006087318003384927
38-39	0.006276561050122283
40-41	0.006402723081280519
42-43	0.006055777495595368
44-45	0.006087318003384927
46-47	0.007727424408442007
48-49	0.007128154760440381
50-51	0.005109562261908592
52-53	0.004825697691802559
54-55	0.005330345816435506
56-57	0.006308101557911842
58-59	0.006308101557911842
60-61	0.004731076168433881
62-63	0.005014940738539914
64-65	0.003343293825693276
66-67	0.003406374841272395
68-69	0.00558266987875198
70-71	0.00438413058274873
72-73	0.0040371849970635785
74-75	0.003658698903588868
76-77	0.0042895090593800525
78-79	0.0028386457010603286
80-81	0.0029017267166394473
82-83	0.004983400230750355
84-85	0.007538181361704651
86-87	0.0058980749566475725
88-89	0.004415671090538289
90-91	0.004731076168433881
92-93	0.006655047143596993
94-95	0.006150399018964046
96-97	0.007317397807177736
98-99	0.006150399018964046
100-101	0.006655047143596993
102-103	0.004762616676223441
104-105	0.004510292613906967
106-107	0.006213480034543164
108-109	0.005614210386541539
110-111	0.006245020542332723
112-113	0.004857238199592119
114-115	0.0023655380842169406
116-117	0.0026809431621125325
118-119	0.13209164662267397
120-121	0.008925963704445256
122-123	0.002081673514110908
124-125	0.001356241834951046
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1585263.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.35617301255232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.78129252237078	50.37212252702551
2	10.452239135604017	13.035232638165889
3	3.389671778710538	6.341008796671208
4	1.637803804830115	4.085087096584124
5	0.9422704432990061	2.9378189393483596
6	0.604453179898784	2.261483223825359
7	0.4151117092450209	1.8119344292853186
8	0.30658776245797803	1.5294111647488773
9	0.24107263654522432	1.3529130329705537
>10	1.164180115126535	12.58369501300853
>50	0.05085466795129505	2.1405824355641263
>100	0.014259999899318799	1.4584147565055388
>500	2.0224406140766866E-4	0.09029594629670855
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.308101557911841E-5	0.0
8	0.0	0.0	0.0	6.308101557911841E-5	0.0
9	0.0	0.0	0.0	6.308101557911841E-5	0.0
10-11	0.0	0.0	0.0	6.308101557911841E-5	0.0
12-13	0.0	0.0	0.0	6.308101557911841E-5	0.0
14-15	0.0	0.0	0.0	6.308101557911841E-5	3.784860934747105E-4
16-17	0.0	0.0	0.0	6.308101557911841E-5	3.784860934747105E-4
18-19	0.0	0.0	0.0	6.308101557911841E-5	4.415671090538289E-4
20-21	0.0	0.0	0.0	6.308101557911841E-5	4.731076168433881E-4
22-23	0.0	0.0	0.0	9.462152336867762E-5	5.677291402120658E-4
24-25	0.0	0.0	0.0	2.2078355452691446E-4	7.885126947389802E-4
26-27	0.0	0.0	0.0	7.885126947389802E-4	9.462152336867762E-4
28-29	0.0	0.0	0.0	0.0038479419503262237	9.462152336867762E-4
30-31	0.0	0.0	0.0	0.013404715810562663	9.777557414763355E-4
32-33	0.0	0.0	0.0	0.03144588626619053	0.0010092962492658946
34-35	0.0	0.0	0.0	0.05223108089951005	0.0010723772648450131
36-37	0.0	0.0	0.0	0.0854432356019159	0.0010723772648450131
38-39	0.0	0.0	0.0	0.14070220524922364	0.0010723772648450131
40-41	0.0	0.0	0.0	0.20860891852014463	0.0010723772648450131
42-43	0.0	0.0	0.0	0.26632804777503793	0.0010723772648450131
44-45	0.0	0.0	0.0	0.3313330343293195	0.0011039177726345725
46-47	0.0	0.0	0.0	0.4031507705661458	0.0011985392960032499
48-49	0.0	0.0	0.0	0.48402063253857563	0.0013877823427406052
50-51	0.0	0.0	0.0	0.5693692466171228	0.0014508633583197237
52-53	0.0	0.0	0.0	0.6542762935866162	0.001640106405057079
54-55	0.0	0.0	0.0	0.7446713889114929	0.0018608899595839933
56-57	0.0	0.0	0.0	0.8330163512300484	0.0018924304673735525
58-59	0.0	0.0	0.0	0.939938672636654	0.0018924304673735525
60-61	0.0	0.0	0.0	1.0555346336853884	0.0018924304673735525
62-63	0.0	0.0	0.0	1.1765240215661377	0.0018924304673735525
64-65	0.0	0.0	0.0	1.28940749894497	0.0018924304673735525
66-67	0.0	0.0	0.0	1.414276369283835	0.0018924304673735525
68-69	0.0	0.0	0.0	1.5620436482779199	0.0018924304673735525
70-71	0.0	0.0	0.0	1.7196831062101368	0.0018924304673735525
72-73	0.0	0.0	0.0	1.8696266802417012	0.002586321638743855
74-75	0.0	0.0	0.0	2.0190971466564225	0.002586321638743855
76-77	0.0	0.0	0.0	2.1948093155520567	0.002586321638743855
78-79	0.0	0.0	0.0	2.4002011022776664	0.002586321638743855
80-81	0.0	0.0	0.0	2.6019657306074766	0.002586321638743855
82-83	0.0	0.0	0.0	2.8142648885389994	0.0026494026543229733
84-85	0.0	0.0	0.0	3.0350169025581244	0.0026494026543229733
86-87	0.0	0.0	0.0	3.273116195861507	0.002712483669902092
88-89	0.0	0.0	0.0	3.542503672892132	0.0027755646854812103
90-91	0.0	0.0	0.0	3.8263367024903756	0.0027755646854812103
92-93	0.0	0.0	0.0	4.116162428568636	0.0027755646854812103
94-95	0.0	0.0	0.0	4.400657808830459	0.0027755646854812103
96-97	0.0	0.0	0.0	4.711174108018669	0.0027755646854812103
98-99	0.0	0.0	0.0	5.068086494165321	0.0027755646854812103
100-101	0.0	0.0	0.0	5.454457714587422	0.0027755646854812103
102-103	0.0	0.0	0.0	5.850764194963233	0.0028071051932707695
104-105	0.0	0.0	0.0	6.25814139357318	0.002870186208849888
106-107	0.0	0.0	0.0	6.683906708224439	0.0029648077322185656
108-109	0.0	0.0	0.0	7.141464854727575	0.0030909697633768026
110-111	0.0	3.154050778955921E-5	0.0	7.650749434005588	0.003311753317903717
112-113	0.0	1.2616203115823683E-4	0.0	8.159403203127809	0.003343293825693276
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1250	0.0	50.941856	1
GTATCAA	2105	0.0	50.040565	1
TCAACGC	2500	0.0	41.89082	4
CAACGCA	2560	0.0	41.372578	5
GTACATG	7495	0.0	40.09782	1
AACGCAG	2720	0.0	38.716476	6
TACATGG	7775	0.0	38.572376	2
ACATGGG	7915	0.0	37.288677	3
ATCAACG	2855	0.0	36.473557	3
TATCAAC	2925	0.0	36.210983	2
CATGGGG	7300	0.0	33.990643	4
ATGGGGG	4480	0.0	30.415152	5
ACGCAGA	3640	0.0	29.093523	7
CGCAGAG	3855	0.0	27.470062	8
GAGTACA	5285	0.0	25.899054	1
AGTACAT	5040	0.0	25.383652	2
TGGGGGG	2990	0.0	23.480223	6
GCAGAGT	4745	0.0	22.191536	9
AGAGTAC	4310	0.0	21.398678	5
GTGGTAT	950	0.0	20.67242	1
>>END_MODULE