FastQCFastQC Report
Mon 15 Aug 2016
SRR2921321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585263
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15920.10042497680195653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.060.804691
GTATCAA26500.049.142911
GTACATG70200.040.676261
TACATGG73200.038.835342
ACATGGG75750.036.8199353
TCAACGC36250.035.1824074
CATGGGG69200.034.7071384
TATCAAC37950.034.3915822
CAACGCA37650.033.7158785
ATCAACG38600.033.503663
AACGCAG39000.032.8564876
ATGGGGG43200.032.281375
GTGGTAT8350.027.186131
TGGGGGG30500.026.5758026
TAGACCG1859.1328184E-725.7714045
ACGCAGA50300.025.1118057
CGCAGAG54400.023.2250582
ATGGGGA27300.022.921685
AGAGTAC54550.022.505695
CAGAGTA59250.021.9274434
Produced by FastQC (version 0.11.5)