##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1585263
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.391830882320473	33.0	30.0	34.0	22.0	34.0
2	30.1313050263584	33.0	31.0	34.0	26.0	34.0
3	30.370119658378453	33.0	31.0	34.0	27.0	34.0
4	32.59034683834796	37.0	35.0	37.0	19.0	37.0
5	32.24828624650925	37.0	35.0	37.0	19.0	37.0
6	32.40997865969243	37.0	35.0	37.0	17.0	37.0
7	32.60962187346831	37.0	35.0	37.0	17.0	37.0
8	32.859605630106806	37.0	35.0	37.0	17.0	37.0
9	34.62827366815475	39.0	37.0	39.0	22.0	39.0
10-11	34.79893651715835	39.0	37.0	39.0	26.0	39.0
12-13	34.826666616201855	39.0	37.0	39.0	26.5	39.0
14-15	36.2235789897323	41.0	38.0	41.0	27.0	41.0
16-17	36.25259878013932	41.0	38.0	41.0	27.0	41.0
18-19	36.27767884571834	41.0	38.0	41.0	27.0	41.0
20-21	36.2772057381015	41.0	38.0	41.0	27.0	41.0
22-23	36.22728531480266	41.0	38.0	41.0	27.0	41.0
24-25	36.17213515990722	41.0	38.0	41.0	27.0	41.0
26-27	36.08391982907568	41.0	38.0	41.0	26.0	41.0
28-29	36.019317930210946	40.0	38.0	41.0	25.5	41.0
30-31	35.93822949251954	40.0	38.0	41.0	25.0	41.0
32-33	35.825213860413065	40.0	37.0	41.0	25.0	41.0
34-35	35.753510616219515	40.0	37.0	41.0	24.0	41.0
36-37	35.67833192347264	40.0	37.0	41.0	24.0	41.0
38-39	35.59012637019851	40.0	37.0	41.0	23.5	41.0
40-41	35.4668077158175	40.0	36.5	41.0	22.5	41.0
42-43	35.32935386746553	40.0	36.0	41.0	20.5	41.0
44-45	35.18845264161215	40.0	36.0	41.0	18.5	41.0
46-47	34.993125746327266	40.0	35.5	41.0	15.5	41.0
48-49	34.79656750961828	40.0	35.0	41.0	11.5	41.0
50-51	34.621490566549525	40.0	35.0	41.0	9.0	41.0
52-53	34.44206576448198	39.5	35.0	41.0	8.0	41.0
54-55	34.23981635854744	39.0	35.0	41.0	7.0	41.0
56-57	34.00083487724119	39.0	35.0	41.0	4.0	41.0
58-59	33.93543027245322	39.0	35.0	41.0	2.0	41.0
60-61	33.69562274524795	38.0	35.0	41.0	2.0	41.0
62-63	33.40629567459784	37.0	34.5	40.5	2.0	41.0
64-65	33.10790922389534	37.0	34.0	40.0	2.0	41.0
66-67	32.78680383002694	36.0	34.0	39.0	2.0	41.0
68-69	32.450635005043324	36.0	34.0	39.0	2.0	41.0
70-71	32.1169704963782	35.0	34.0	38.5	2.0	40.5
72-73	31.771127882250454	35.0	33.5	37.0	2.0	39.5
74-75	31.43811216183056	35.0	33.0	37.0	2.0	39.0
76-77	31.136555574690128	35.0	33.0	36.5	2.0	39.0
78-79	30.872959565699823	35.0	33.0	36.0	2.0	37.5
80-81	30.642928018883932	35.0	33.0	36.0	2.0	37.0
82-83	30.4399680053089	35.0	33.0	35.0	2.0	37.0
84-85	30.264609090100507	35.0	33.0	35.0	2.0	36.0
86-87	30.122712130416215	35.0	33.0	35.0	2.0	36.0
88-89	30.004110358975137	35.0	32.5	35.0	2.0	36.0
90-91	29.90012698208436	35.0	32.0	35.0	2.0	35.5
92-93	29.833568625521444	35.0	32.0	35.0	2.0	35.0
94-95	29.767977300927353	35.0	32.0	35.0	2.0	35.0
96-97	29.70064935597437	35.0	32.0	35.0	2.0	35.0
98-99	29.645433281417656	35.0	32.0	35.0	2.0	35.0
100-101	29.59285147007153	35.0	32.0	35.0	2.0	35.0
102-103	28.913493533880498	34.0	30.5	35.0	2.0	35.0
104-105	29.469648884759184	35.0	32.0	35.0	2.0	35.0
106-107	29.52882770871458	35.0	32.0	35.0	2.0	35.0
108-109	29.6009523340922	35.0	32.0	35.0	2.0	35.0
110-111	29.710222846303736	35.0	33.0	35.0	2.0	35.0
112-113	29.67561250089102	35.0	33.0	35.0	2.0	35.0
114-115	29.61381770721956	35.0	33.0	35.0	2.0	35.0
116-117	29.55205729270159	35.0	33.0	35.0	2.0	35.0
118-119	29.47731417436728	35.0	32.5	35.0	2.0	35.0
120-121	29.384885347100134	35.0	32.0	35.0	2.0	35.0
122-123	29.274530472230794	35.0	32.0	35.0	2.0	35.0
124-125	28.750702249405933	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101688.0
3	5930.0
4	4402.0
5	4138.0
6	4357.0
7	4669.0
8	4938.0
9	4886.0
10	5048.0
11	5119.0
12	5595.0
13	6430.0
14	7772.0
15	6677.0
16	4705.0
17	4102.0
18	3677.0
19	3450.0
20	3329.0
21	3358.0
22	3915.0
23	4870.0
24	6331.0
25	8165.0
26	9723.0
27	11216.0
28	13851.0
29	16264.0
30	20017.0
31	24620.0
32	31928.0
33	43554.0
34	66423.0
35	112570.0
36	259913.0
37	560676.0
38	196710.0
39	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.66227925880561	17.500999630516315	11.534850409721976	27.301870700956105
2	15.948899330899668	19.02416192139727	37.79051173212268	27.23642701558038
3	19.47008162052606	22.351622412180188	28.2870413300506	29.891254637243158
4	13.51184756679615	15.258903892659283	31.249223190530778	39.98002535001379
5	14.418679290700473	37.39181362660246	32.3698304461521	15.81967663654496
6	35.399548039854785	33.468904548819204	15.740796250030764	15.390751161295249
7	30.800602666572445	31.490464088592994	18.244859794215106	19.46407345061945
8	28.764703581598805	35.17207748375872	17.25531461217115	18.807904322471323
9	27.78480465662324	15.766696968110306	17.605033614415497	38.84346476085096
10-11	27.306335795855617	25.515713482766866	25.10037969229721	22.077571029080303
12-13	26.82770116531314	22.513934463995717	26.126985870191575	24.531378500499564
14-15	24.332429382380084	24.028915075921155	23.18567960016729	28.452975941531466
16-17	24.552172100149942	27.088186628969453	23.974665402523115	24.38497586835749
18-19	24.71220863667417	26.03877085379524	25.680092199212375	23.568928310318224
20-21	25.201212669443496	25.055810928533624	25.67889365991637	24.064082742106514
22-23	25.0691841038364	24.666727224441622	25.550271469150545	24.713817202571434
24-25	24.64121095363987	25.113813922358624	25.506682487385373	24.738292636616134
26-27	24.40131385139248	25.165288031071185	25.70740627895813	24.725991838578203
28-29	24.445628264836813	25.567461045895858	25.60710746418733	24.379803225080003
30-31	24.941697371350998	25.337877689695652	25.496368741338188	24.22405619761516
32-33	24.48044898543649	25.47249257694149	25.580140330027255	24.466918107594765
34-35	24.642819519537138	25.532703406311757	25.787109142142345	24.037367932008756
36-37	24.800585139500512	25.34525816851841	25.801807018772276	24.0523496732088
38-39	24.5722318631041	25.58077114018305	25.854258883226315	23.99273811348653
40-41	24.425915447468338	25.696304020216203	25.743047052760332	24.13473347955513
42-43	24.41030289611251	25.675960392691938	25.499775116179464	24.413961595016094
44-45	24.21377399207576	25.701697447048215	25.828458747854455	24.25606981302156
46-47	24.2317836220236	25.633317626160455	25.377807972557235	24.757090779258707
48-49	24.262125590517158	25.91118949978647	25.286214337936354	24.540470571760018
50-51	24.23165745999244	25.964461417443037	25.48813666880511	24.315744453759407
52-53	24.385291273435385	25.86409952165666	25.402819595234355	24.3477896096736
54-55	24.048375569227314	25.5467704727859	25.702422878727376	24.702431079259405
56-57	24.120981818158878	25.74881896568582	25.450477302504382	24.67972191365092
58-59	24.172897493980493	25.787172223157924	25.598307662514042	24.441622620347538
60-61	24.563495142446396	25.56348694191437	25.64867785345397	24.224340062185266
62-63	24.302402818964424	25.648110124313757	25.623887014331377	24.425600042390442
64-65	24.13593201885113	25.431994564939696	25.646911585017758	24.78516183119142
66-67	24.096254060051866	25.731503226909354	25.488641316929744	24.683601396109037
68-69	23.910985117295994	25.577522467880726	25.685107139950908	24.82638527487237
70-71	23.93930849329102	25.482900944512043	25.727655284959027	24.850135277237907
72-73	24.03087058740411	25.756325606539736	25.372477626740796	24.840326179315355
74-75	23.794947589138207	25.840065654721016	25.51980333862583	24.84518341751495
76-77	23.905497068940612	25.3615961515534	25.579162574285778	25.153744205220207
78-79	23.804945930107497	25.438334207005397	25.77042421352167	24.986295649365438
80-81	24.00797217874889	25.676843526910048	25.3280685917731	24.987115702567966
82-83	24.07726667436255	25.669778453165183	25.469370066670326	24.78358480580194
84-85	24.009769987692895	25.704851497827175	25.31150982518358	24.97386868929635
86-87	23.649861253306234	25.892454438159472	25.518794042376562	24.938890266157728
88-89	23.630842327109132	25.576797036201565	25.584082893500952	25.208277743188358
90-91	23.929684587420212	25.384906043625733	25.586513732473666	25.098895636480396
92-93	23.78369802732177	25.64544780149074	25.5911348072488	24.979719363938695
94-95	23.835025117338834	25.45147971041965	25.51935503304033	25.19414013920118
96-97	23.60252855059372	25.38892771892994	25.58962085618979	25.418922874286547
98-99	23.51558402194212	25.480717219209577	25.43492035856545	25.568778400282856
100-101	23.468566414531846	25.519424852532357	25.5869530797098	25.425055653225993
102-103	23.274749994165006	25.44628241496837	25.658234627314208	25.620732963552417
104-105	23.441558380115836	25.466839382650747	25.552061915337	25.53954032189642
106-107	23.431029251371545	25.407078590943183	25.6456515938405	25.51624056384477
108-109	23.428424178807713	25.346442467336505	25.620183743193532	25.604949610662253
110-111	23.27030139324632	25.699753572691325	25.551260065812777	25.478684968249578
112-113	23.230225614042947	25.726580293410855	25.558089678514733	25.48510441403146
114-115	23.257716663160394	25.691111427218782	25.52290499727331	25.528266912347515
116-117	22.96094786161826	25.506031373550904	25.688052849624903	25.844967915205935
118-119	23.0701539688227	25.387098361400305	25.725151016618135	25.81759665315886
120-121	23.016508333017928	25.41138362161412	25.783846182960524	25.78826186240743
122-123	22.859751741452804	25.673264500541716	25.52196272809996	25.94502102990552
124-125	22.740409377770643	25.475292846376256	25.462077344434775	26.322220431418327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	42.5
2	58.5
3	66.5
4	55.5
5	78.5
6	191.0
7	390.0
8	518.0
9	611.5
10	609.5
11	523.0
12	517.5
13	552.0
14	626.5
15	678.0
16	722.0
17	764.0
18	797.0
19	864.0
20	937.5
21	997.5
22	1070.5
23	1216.5
24	1430.0
25	1675.0
26	2040.5
27	2583.0
28	3275.0
29	4216.5
30	5541.5
31	7084.0
32	9095.0
33	11444.5
34	14048.5
35	16798.0
36	20093.5
37	24035.0
38	27605.5
39	31173.0
40	34804.0
41	38014.5
42	41504.5
43	46148.0
44	50871.5
45	55625.0
46	59718.5
47	63531.5
48	68347.5
49	71789.5
50	73731.5
51	75543.0
52	75307.0
53	73563.5
54	72767.0
55	70696.0
56	66334.5
57	60810.0
58	55488.5
59	49591.5
60	41924.5
61	34446.5
62	27797.5
63	21103.5
64	15243.0
65	11236.5
66	8370.0
67	6302.5
68	4759.0
69	3529.5
70	2628.5
71	1963.0
72	1395.5
73	983.5
74	728.0
75	542.5
76	409.0
77	298.5
78	251.5
79	204.0
80	160.0
81	159.5
82	146.5
83	131.5
84	124.0
85	120.0
86	134.0
87	125.0
88	101.0
89	100.0
90	104.0
91	91.0
92	79.0
93	83.0
94	73.5
95	52.5
96	44.0
97	39.0
98	29.5
99	14.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29515607189469506
2	0.0
3	0.0
4	0.016022577957096076
5	0.15530546035578954
6	0.03847941950326224
7	0.019365871782789354
8	0.006623506635807434
9	0.005046481246329473
10-11	0.0020185924985317892
12-13	0.055637455740782445
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.784860934747105E-4
92-93	4.415671090538289E-4
94-95	2.2078355452691446E-4
96-97	4.415671090538289E-4
98-99	9.462152336867762E-5
100-101	0.0
102-103	0.0
104-105	9.462152336867762E-5
106-107	2.5232406231647366E-4
108-109	4.415671090538289E-4
110-111	5.361886324225066E-4
112-113	7.254316791598618E-4
114-115	5.361886324225066E-4
116-117	6.623506635807434E-4
118-119	4.415671090538289E-4
120-121	1.8924304673735524E-4
122-123	0.0012931608193719275
124-125	2.2078355452691446E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1585263.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.23841737419383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.73846749787927	53.149882080299385
2	9.298360404115543	11.946239130705036
3	3.0576290646662145	5.8925175609448255
4	1.501569929285149	3.858339033358324
5	0.8570752315774961	2.752857822357951
6	0.539594294120508	2.079761008706802
7	0.3749326429777427	1.6859555724769688
8	0.28922341716823907	1.4863403669155133
9	0.2019987223508875	1.1678470411887185
>10	1.0773950627581823	12.01743123690671
>50	0.049014143996078174	2.1284353386140418
>100	0.014248326965592568	1.5010792431020998
>500	1.9650485563807243E-4	0.08242924088944834
>1k	2.947572834571086E-4	0.25088532353411724
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1592	0.10042497680195653	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	6.308101557911841E-5
5	0.0	0.0	0.0	0.0	6.308101557911841E-5
6	0.0	0.0	0.0	0.0	6.308101557911841E-5
7	0.0	0.0	0.0	0.0	1.2616203115823683E-4
8	0.0	0.0	0.0	0.0	1.2616203115823683E-4
9	0.0	0.0	0.0	0.0	2.5232406231647366E-4
10-11	0.0	0.0	0.0	0.0	2.5232406231647366E-4
12-13	0.0	0.0	0.0	0.0	2.838645701060329E-4
14-15	0.0	0.0	0.0	0.0	4.415671090538289E-4
16-17	0.0	0.0	0.0	0.0	5.046481246329473E-4
18-19	0.0	0.0	0.0	0.0	5.046481246329473E-4
20-21	0.0	0.0	0.0	0.0	5.046481246329473E-4
22-23	0.0	0.0	0.0	0.0	5.677291402120658E-4
24-25	0.0	0.0	0.0	9.462152336867762E-5	6.308101557911842E-4
26-27	0.0	0.0	0.0	6.938911713703026E-4	6.308101557911842E-4
28-29	0.0	0.0	0.0	0.0035956178880097497	6.308101557911842E-4
30-31	0.0	0.0	0.0	0.012900067685929717	6.308101557911842E-4
32-33	0.0	0.0	0.0	0.02993194189229169	6.938911713703026E-4
34-35	0.0	0.0	0.0	0.04955013773739751	6.938911713703026E-4
36-37	6.308101557911841E-5	0.0	0.0	0.08068061892569245	6.938911713703026E-4
38-39	6.308101557911841E-5	0.0	0.0	0.13347942896541456	6.938911713703026E-4
40-41	6.308101557911841E-5	0.0	0.0	0.19659198505232256	6.938911713703026E-4
42-43	6.308101557911841E-5	0.0	0.0	0.2505893343880479	6.938911713703026E-4
44-45	6.308101557911841E-5	0.0	0.0	0.31080016375831643	7.254316791598617E-4
46-47	6.308101557911841E-5	0.0	0.0	0.3771929326553386	7.56972186949421E-4
48-49	6.308101557911841E-5	0.0	0.0	0.45276398931912243	8.200532025285395E-4
50-51	6.308101557911841E-5	0.0	0.0	0.5335707702759731	8.200532025285395E-4
52-53	6.308101557911841E-5	0.0	0.0	0.6138098220926118	8.200532025285395E-4
54-55	6.308101557911841E-5	0.0	0.0	0.6995369222646337	8.200532025285395E-4
56-57	6.308101557911841E-5	0.0	0.0	0.7835292945082298	8.200532025285395E-4
58-59	6.308101557911841E-5	0.0	0.0	0.8851843511139792	8.200532025285395E-4
60-61	6.308101557911841E-5	0.0	0.0	0.995607668885226	8.200532025285395E-4
62-63	6.308101557911841E-5	0.0	0.0	1.1115821160274353	8.831342181076578E-4
64-65	6.308101557911841E-5	0.0	0.0	1.2179051677860393	8.831342181076578E-4
66-67	6.308101557911841E-5	0.0	0.0	1.335267397270989	8.831342181076578E-4
68-69	6.308101557911841E-5	0.0	0.0	1.4754334138877903	8.831342181076578E-4
70-71	6.308101557911841E-5	0.0	0.0	1.6251562043648278	9.462152336867762E-4
72-73	6.308101557911841E-5	0.0	0.0	1.7669307868788966	0.0011354582804241316
74-75	6.308101557911841E-5	0.0	0.0	1.9092730985331772	0.0011354582804241316
76-77	6.308101557911841E-5	0.0	0.0	2.077289383528159	0.0011354582804241316
78-79	6.308101557911841E-5	0.0	0.0	2.2724620457299514	0.0011354582804241316
80-81	6.308101557911841E-5	0.0	0.0	2.4618627950062546	0.0011985392960032499
82-83	6.308101557911841E-5	0.0	0.0	2.6604102915415297	0.0012616203115823684
84-85	6.308101557911841E-5	0.0	0.0	2.8686407239681992	0.0012616203115823684
86-87	6.308101557911841E-5	0.0	0.0	3.093240679937651	0.0012931608193719275
88-89	6.308101557911841E-5	0.0	0.0	3.3464163359644425	0.0013247013271614867
90-91	6.308101557911841E-5	0.0	0.0	3.6141321660822214	0.0013247013271614867
92-93	6.308101557911841E-5	0.0	0.0	3.886452910337275	0.0013247013271614867
94-95	6.308101557911841E-5	0.0	0.0	4.154011037916106	0.0014508633583197237
96-97	6.308101557911841E-5	0.0	0.0	4.4468331122343745	0.0015454848816884013
98-99	6.308101557911841E-5	0.0	0.0	4.7858620304643456	0.001640106405057079
100-101	6.308101557911841E-5	0.0	0.0	5.1510695701596525	0.001640106405057079
102-103	6.308101557911841E-5	0.0	0.0	5.528357124338359	0.001640106405057079
104-105	6.308101557911841E-5	0.0	0.0	5.913403643433298	0.001640106405057079
106-107	6.308101557911841E-5	0.0	0.0	6.319929248332926	0.001671646912846638
108-109	6.308101557911841E-5	0.0	0.0	6.756607578679374	0.0017347279284257566
110-111	6.308101557911841E-5	0.0	0.0	7.2432460733644835	0.0017662684362153157
112-113	6.308101557911841E-5	0.0	0.0	7.725942004575897	0.0017662684362153157
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1680	0.0	60.80469	1
GTATCAA	2650	0.0	49.14291	1
GTACATG	7020	0.0	40.67626	1
TACATGG	7320	0.0	38.83534	2
ACATGGG	7575	0.0	36.819935	3
TCAACGC	3625	0.0	35.182407	4
CATGGGG	6920	0.0	34.707138	4
TATCAAC	3795	0.0	34.391582	2
CAACGCA	3765	0.0	33.715878	5
ATCAACG	3860	0.0	33.50366	3
AACGCAG	3900	0.0	32.856487	6
ATGGGGG	4320	0.0	32.28137	5
GTGGTAT	835	0.0	27.18613	1
TGGGGGG	3050	0.0	26.575802	6
TAGACCG	185	9.1328184E-7	25.771404	5
ACGCAGA	5030	0.0	25.111805	7
CGCAGAG	5440	0.0	23.225058	2
ATGGGGA	2730	0.0	22.92168	5
AGAGTAC	5455	0.0	22.50569	5
CAGAGTA	5925	0.0	21.927443	4
>>END_MODULE