FastQCFastQC Report
Mon 15 Aug 2016
SRR2921320_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921320_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011593
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27500.056.2618681
GGTATCA15750.055.9183921
TCAACGC34950.043.0749974
CAACGCA36600.040.969495
GTACATG101000.040.0057031
AACGCAG38650.038.9484836
TATCAAC39200.038.4048772
TACATGG106200.037.5967182
ACATGGG105700.037.26793
ATCAACG41500.036.4197963
CATGGGG96300.034.9737364
ATGGGGG59950.033.6474085
ACGCAGA49750.030.0147937
CGCAGAG53600.027.7478878
TGGGGGG41100.026.6376256
GTATACG1200.001287221624.7948931
GAGTACA70600.024.3594021
GTATAGG5750.023.8030971
AGTACAT69650.023.665222
GCAGAGT64050.023.034959
Produced by FastQC (version 0.11.5)