##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921320_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2011593
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.753555515454668	31.0	31.0	34.0	28.0	34.0
2	30.835555204258515	33.0	31.0	34.0	27.0	34.0
3	31.05696878046404	34.0	31.0	34.0	28.0	34.0
4	34.44793504451447	37.0	35.0	37.0	32.0	37.0
5	34.51962300525007	37.0	35.0	37.0	32.0	37.0
6	34.584308555458286	37.0	35.0	37.0	32.0	37.0
7	34.52124907970946	37.0	35.0	37.0	32.0	37.0
8	34.53284237914926	37.0	35.0	37.0	32.0	37.0
9	36.167082009134056	39.0	37.0	39.0	32.0	39.0
10-11	36.16016758857283	39.0	37.0	39.0	32.0	39.0
12-13	36.089356296228914	39.0	37.0	39.0	32.0	39.0
14-15	37.44121325735375	41.0	38.0	41.0	32.0	41.0
16-17	37.42364036860339	41.0	38.0	41.0	32.0	41.0
18-19	37.416989917940654	41.0	38.0	41.0	32.0	41.0
20-21	37.44160598093153	41.0	38.0	41.0	32.0	41.0
22-23	37.38945129556525	41.0	38.0	41.0	31.5	41.0
24-25	37.3665122616752	41.0	38.0	41.0	31.0	41.0
26-27	37.31818787398843	41.0	38.0	41.0	31.0	41.0
28-29	37.24660405956871	40.5	38.0	41.0	31.0	41.0
30-31	37.17046589444286	40.0	38.0	41.0	30.5	41.0
32-33	37.090275716807525	40.0	38.0	41.0	30.0	41.0
34-35	36.98249894486608	40.0	38.0	41.0	30.0	41.0
36-37	36.88615937717023	40.0	38.0	41.0	30.0	41.0
38-39	36.79060376527458	40.0	38.0	41.0	30.0	41.0
40-41	36.68951597067598	40.0	37.0	41.0	30.0	41.0
42-43	36.516368867857466	40.0	37.0	41.0	30.0	41.0
44-45	36.30963942507257	40.0	36.5	41.0	29.0	41.0
46-47	36.107140957440194	40.0	36.0	41.0	28.5	41.0
48-49	35.90283074160628	40.0	35.5	41.0	28.0	41.0
50-51	35.731485693179486	39.5	35.0	41.0	28.0	41.0
52-53	35.53449132105749	39.0	35.0	41.0	27.5	41.0
54-55	35.32007394139868	39.0	35.0	41.0	27.0	41.0
56-57	35.063230981615064	39.0	35.0	40.5	26.5	41.0
58-59	34.784709431778694	38.0	35.0	40.0	26.0	41.0
60-61	34.492473875182505	37.5	34.0	40.0	26.0	41.0
62-63	34.16647303902927	37.0	34.0	40.0	26.0	41.0
64-65	33.810112433280494	36.5	34.0	39.5	25.0	41.0
66-67	33.46433697074906	36.0	34.0	39.0	24.5	41.0
68-69	33.10692023684712	35.5	33.0	39.0	24.0	40.0
70-71	33.04128792454537	35.0	34.0	38.5	25.0	40.0
72-73	32.74263009465632	35.0	33.5	37.0	24.5	39.5
74-75	32.41702073928474	35.0	33.0	37.0	24.0	39.0
76-77	32.118861767763164	35.0	33.0	36.5	23.5	39.0
78-79	31.838396484776	35.0	33.0	36.0	23.0	37.5
80-81	31.567065007682967	35.0	33.0	35.5	21.5	37.0
82-83	31.348184747113358	35.0	33.0	35.0	20.5	37.0
84-85	31.139041794239688	35.0	33.0	35.0	20.0	36.0
86-87	30.97779247591337	35.0	33.0	35.0	20.0	36.0
88-89	30.839941280368343	35.0	32.5	35.0	19.0	36.0
90-91	30.712189294752967	35.0	32.0	35.0	18.0	35.0
92-93	30.6030832280685	35.0	32.0	35.0	18.0	35.0
94-95	30.479190621562115	35.0	32.0	35.0	16.5	35.0
96-97	30.38503837505897	35.0	32.0	35.0	14.0	35.0
98-99	30.300584163893987	35.0	32.0	35.0	10.5	35.0
100-101	30.199179953400115	35.0	32.0	35.0	7.5	35.0
102-103	29.366363126139333	33.5	30.0	35.0	7.0	35.0
104-105	29.947338005252554	34.5	31.0	35.0	6.0	35.0
106-107	30.013133123847616	35.0	32.0	35.0	2.0	35.0
108-109	29.939235471588937	35.0	31.5	35.0	2.0	35.0
110-111	29.845420022837622	35.0	31.0	35.0	2.0	35.0
112-113	29.745759206758024	35.0	31.0	35.0	2.0	35.0
114-115	29.612480506742667	34.5	31.0	35.0	2.0	35.0
116-117	29.476350832399994	34.0	31.0	35.0	2.0	35.0
118-119	29.469445608530155	34.5	31.0	35.0	2.0	35.0
120-121	29.630067563368932	35.0	32.0	35.0	2.0	35.0
122-123	29.48146543560253	35.0	32.0	35.0	2.0	35.0
124-125	28.908944055780665	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57037.0
3	8747.0
4	5496.0
5	4801.0
6	5027.0
7	5577.0
8	5888.0
9	6285.0
10	6850.0
11	7275.0
12	7316.0
13	7184.0
14	7231.0
15	6860.0
16	5749.0
17	5415.0
18	5180.0
19	4989.0
20	5261.0
21	5510.0
22	6344.0
23	8102.0
24	11042.0
25	14267.0
26	16237.0
27	18127.0
28	21751.0
29	26273.0
30	32148.0
31	39941.0
32	52295.0
33	70283.0
34	107431.0
35	180224.0
36	390118.0
37	665696.0
38	177578.0
39	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.008096272254306	16.885828838718915	11.810892081523555	25.29518280750322
2	16.59469257800148	18.610779109980648	38.80878325839278	25.985745053625088
3	20.070260952917902	22.96805294330848	28.028512065722726	28.93317403805089
4	13.818828150247347	14.210952842618141	34.38526362890596	37.58495537822855
5	14.92524179459466	37.24994009201469	32.80556304060121	15.019255072789445
6	35.38422217503877	33.047597215999694	16.50147210703991	15.066708501921621
7	30.954548415018973	31.080284923203283	19.17514066430043	18.790025997477315
8	28.07605262948556	34.890600513885246	18.519159328289938	18.51418752833926
9	28.132292740743097	15.269625706839651	17.87321183364174	38.72486971877551
10-11	27.170283526226157	24.809856040695294	26.18745359348903	21.832406839589517
12-13	26.709009086940917	22.067997442651052	26.620416633108917	24.602576837299114
14-15	24.221911559120006	23.461248202805116	23.49473121123478	28.8221090268401
16-17	24.807233216239837	26.635895238275154	24.17775210315894	24.379119442326065
18-19	24.837593656768917	26.10883002306277	25.497534883744056	23.55604143642426
20-21	25.528882304469462	24.994202037551904	25.28088114114838	24.19603451683025
22-23	25.482661377022612	24.585534762382377	25.08924410041872	24.84255976017629
24-25	25.007140280853534	24.86724297661095	25.145098709877402	24.980518032658118
26-27	24.776869940019736	25.06287611950316	25.262081188369844	24.898172752107264
28-29	24.815017080795908	25.51845802285344	25.087081857318744	24.57944303903191
30-31	25.329781723127525	25.121825599234793	25.028461601943242	24.519931075694437
32-33	24.95309941581522	25.080222557472016	25.063542927426706	24.903135099286057
34-35	24.960438731116113	25.296740588942136	25.28806519915861	24.45475548078314
36-37	24.96836239899396	25.33257699725795	25.16856608275169	24.530494520996406
38-39	25.04408509081255	25.411630708379175	25.083956805311193	24.46032739549708
40-41	25.068837174385934	25.153154478896933	25.15263246728292	24.62537587943421
42-43	24.761434596575953	25.16390535088982	25.132659242670897	24.94200080986333
44-45	24.66793805653343	25.2308909953879	25.327015824926036	24.774155123152635
46-47	24.706773443955345	25.170129073378227	25.168438720223683	24.95465876244274
48-49	24.561704420938756	25.299538437368874	25.222081029000293	24.916676112692077
50-51	24.582787817804483	25.324680651252944	25.336413326230755	24.756118204711814
52-53	24.766845199729254	25.27475340985555	25.261529352312014	24.696872038103184
54-55	24.443404298052815	24.979828175328578	25.53504428852744	25.04172323809117
56-57	24.60326969408017	25.169579833770996	25.139825060827008	25.087325411321824
58-59	24.61513489139559	25.323081366863683	25.327158024938708	24.734625716802018
60-61	24.989733957879896	25.107209297007287	25.293315489145833	24.609741255966984
62-63	24.81378111705975	25.202873311501943	25.303719605046442	24.67962596639186
64-65	24.649194586764	24.907905189822195	25.379241705501425	25.06365851791238
66-67	24.64083547248247	25.244014628822097	25.152093565600147	24.963056333095288
68-69	24.532031925037607	25.113052098423932	25.330928449058447	25.023987527480013
70-71	24.443616425498554	25.029250639263495	25.472801441912967	25.054331493324987
72-73	24.563186676609494	25.32875963211534	25.069326373353217	25.03872731792195
74-75	24.320466793718847	25.34917185327735	25.257673403978455	25.072687949025347
76-77	24.413385241553687	24.95193882771008	25.311720264340178	25.32295566639606
78-79	24.300734543478654	24.944625250090542	25.472534099188103	25.2821061072427
80-81	24.497123079691736	25.25607424413107	25.17061673575186	25.076185940425326
82-83	24.559218686691672	25.291786913648373	25.23386950437115	24.915124895288805
84-85	24.56352822617732	25.30126831237543	25.087289383135143	25.04791407831211
86-87	24.264579949066658	25.405969069615114	25.24737720627258	25.082073775045643
88-89	24.334897344958524	25.09159858294382	25.27894689221047	25.29455717988719
90-91	24.538127992809304	24.91113543804388	25.36405884598864	25.18667772315818
92-93	24.451331175505665	25.19833462304179	25.355038550806846	24.995295650645698
94-95	24.475161344929273	25.059242044861634	25.282936227893142	25.18266038231595
96-97	24.258866776738543	25.021788165831683	25.397966504144105	25.321378553285673
98-99	24.190908666069422	25.004797524576748	25.292673856821978	25.51161995253185
100-101	24.243094653282824	25.02500086381309	25.413878331863813	25.318026151040268
102-103	24.032274702862193	24.992785172130503	25.51951110685775	25.45542901814955
104-105	24.135909891873926	25.018015212072413	25.45913001502117	25.386944881032488
106-107	24.103139654403112	24.891179113548827	25.561591838713166	25.444089393334902
108-109	24.14241311526266	24.916478494703842	25.579529873355543	25.36157851667795
110-111	23.95802209264114	25.293260198295847	25.373799553454017	25.374918155608995
112-113	23.961644267383484	25.287679180386835	25.51499874098024	25.235677811249445
114-115	24.008213629942713	25.35094476381091	25.34627171445481	25.294569891791564
116-117	23.77814505089804	25.213824460737804	25.54819821712072	25.45983227124343
118-119	23.86348363099831	25.051920046115754	25.564450050725334	25.5201462721606
120-121	23.734764851085195	25.108917487204092	25.696099951028767	25.460217710681942
122-123	23.72984320991398	25.331921813254798	25.476238887262166	25.46199608956905
124-125	23.64407002947474	25.138760947387418	25.52975238534605	25.68741663779179
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	258.0
1	217.5
2	172.0
3	150.5
4	126.5
5	117.5
6	121.5
7	141.5
8	185.5
9	221.0
10	215.0
11	211.5
12	251.0
13	281.0
14	298.0
15	341.0
16	390.5
17	430.0
18	447.5
19	511.0
20	568.5
21	617.5
22	736.0
23	878.5
24	1038.5
25	1224.0
26	1569.0
27	2202.0
28	3006.5
29	4111.5
30	5749.5
31	7588.0
32	10019.0
33	13041.5
34	16361.0
35	19867.0
36	24271.5
37	28852.0
38	32977.0
39	37736.0
40	42282.5
41	46145.0
42	50640.0
43	56943.5
44	63627.0
45	70219.0
46	75271.5
47	80183.5
48	87200.5
49	92295.0
50	94022.5
51	95299.0
52	95638.5
53	94317.0
54	94248.5
55	92632.0
56	86681.5
57	79374.5
58	72761.5
59	65889.0
60	57002.0
61	47386.0
62	38869.5
63	30101.5
64	21800.5
65	16162.0
66	12173.5
67	9072.5
68	6860.0
69	5146.0
70	3856.5
71	2874.0
72	2128.5
73	1562.5
74	1132.5
75	796.5
76	536.0
77	350.0
78	200.5
79	126.5
80	90.5
81	71.0
82	49.5
83	36.0
84	31.5
85	24.5
86	19.0
87	16.0
88	13.5
89	13.5
90	11.5
91	8.5
92	4.5
93	5.0
94	5.5
95	2.0
96	1.5
97	2.5
98	1.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014764418050768719
2	0.010986317808821169
3	0.011632571797575354
4	0.012079978405174406
5	0.008699572925537124
6	0.02634727800305529
7	0.01212969025046319
8	0.012378249476907108
9	0.008699572925537124
10-11	0.007779903787694628
12-13	0.006711099113985781
14-15	0.006213980661097946
16-17	0.004722625302434439
18-19	0.006263692506386729
20-21	0.005493158904410584
22-23	0.0057417181308545015
24-25	0.005841141821432069
26-27	0.004871760838300789
28-29	0.006139412893164771
30-31	0.005518014827054976
32-33	0.00782961563298341
34-35	0.007755047865050236
36-37	0.007034226108362874
38-39	0.007059082031007267
40-41	0.007133649798940441
42-43	0.007083937953651659
44-45	0.007257929412162401
46-47	0.008898420306692259
48-49	0.00820245447264929
50-51	0.005791429976143285
52-53	0.005344023368544233
54-55	0.006164268815809162
56-57	0.0075064886386063175
58-59	0.007034226108362874
60-61	0.005319167445899842
62-63	0.005766574053498894
64-65	0.003927235777813902
66-67	0.0042006509269022115
68-69	0.006512251732830647
70-71	0.004896616760945181
72-73	0.004623201611856871
74-75	0.004150939081613427
76-77	0.004672913457145655
78-79	0.0035543969381480246
80-81	0.003330693634348499
82-83	0.005368879291188625
84-85	0.008948132151981042
86-87	0.006984514263074091
88-89	0.0049214726835895735
90-91	0.005219743755322274
92-93	0.007779903787694628
94-95	0.007034226108362874
96-97	0.008948132151981042
98-99	0.006909946495140915
100-101	0.008078174859427328
102-103	0.005493158904410584
104-105	0.004896616760945181
106-107	0.007233073489518009
108-109	0.006711099113985781
110-111	0.00765562417447267
112-113	0.005344023368544233
114-115	0.0029081429493938386
116-117	0.0035543969381480246
118-119	0.13596189686482305
120-121	0.010340063820066981
122-123	0.0024855922644391785
124-125	0.0019884738115513428
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2011593.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.40921231045944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93107110244257	45.31370618812095
2	10.92317997482256	12.541823165598945
3	3.7561623025650905	6.469149573015103
4	1.8844093562034563	4.3272982724040165
5	1.0525213959795376	3.0212212141545205
6	0.7081141360495403	2.439136486590337
7	0.48876652176003094	1.9641790712576486
8	0.36354266040021055	1.669655821985995
9	0.2746053552995817	1.4188389429584551
>10	1.520577667696685	15.452822612295586
>50	0.07165190646665805	2.8066944211993166
>100	0.024444749663215897	2.2214427783083606
>500	9.52870650757052E-4	0.3540314521107007
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.942369057756714E-5
2	0.0	0.0	0.0	0.0	9.942369057756714E-5
3	0.0	0.0	0.0	0.0	9.942369057756714E-5
4	0.0	0.0	0.0	0.0	1.491355358663507E-4
5	0.0	0.0	0.0	0.0	1.491355358663507E-4
6	0.0	0.0	0.0	0.0	1.491355358663507E-4
7	0.0	0.0	0.0	0.0	1.491355358663507E-4
8	0.0	0.0	0.0	0.0	1.9884738115513427E-4
9	0.0	0.0	0.0	0.0	3.4798291702148494E-4
10-11	0.0	0.0	0.0	0.0	3.4798291702148494E-4
12-13	0.0	0.0	0.0	0.0	3.7283883966587677E-4
14-15	4.971184528878357E-5	0.0	0.0	0.0	4.971184528878357E-4
16-17	4.971184528878357E-5	0.0	0.0	9.942369057756714E-5	5.468302981766192E-4
18-19	4.971184528878357E-5	0.0	0.0	1.7399145851074247E-4	5.965421434654028E-4
20-21	4.971184528878357E-5	0.0	0.0	1.9884738115513427E-4	5.965421434654028E-4
22-23	4.971184528878357E-5	0.0	0.0	2.2370330379952607E-4	6.711099113985782E-4
24-25	4.971184528878357E-5	0.0	0.0	4.971184528878357E-4	7.456776793317535E-4
26-27	4.971184528878357E-5	0.0	0.0	9.942369057756714E-4	8.699572925537124E-4
28-29	4.971184528878357E-5	0.0	0.0	0.003554396938148025	8.948132151981042E-4
30-31	4.971184528878357E-5	0.0	0.0	0.01252738501277346	8.948132151981042E-4
32-33	4.971184528878357E-5	0.0	0.0	0.030995335537556554	8.948132151981042E-4
34-35	4.971184528878357E-5	0.0	0.0	0.05239628493437788	9.445250604868877E-4
36-37	4.971184528878357E-5	0.0	0.0	0.08771655101205861	9.445250604868877E-4
38-39	4.971184528878357E-5	0.0	0.0	0.1493343832475058	9.445250604868877E-4
40-41	4.971184528878357E-5	0.0	0.0	0.219005534419736	9.445250604868877E-4
42-43	4.971184528878357E-5	0.0	0.0	0.28216443385913553	9.693809831312796E-4
44-45	4.971184528878357E-5	0.0	0.0	0.3536749233070507	0.001019092828420063
46-47	4.971184528878357E-5	0.0	0.0	0.4346306633598347	0.0010688046737088465
48-49	4.971184528878357E-5	0.0	0.0	0.5213281215434733	0.0010936605963532385
50-51	4.971184528878357E-5	0.0	0.0	0.6093926554725533	0.0010936605963532385
52-53	4.971184528878357E-5	0.0	0.0	0.6980537315450988	0.0013173639001527646
54-55	4.971184528878357E-5	0.0	0.0	0.7950663976261599	0.0014416435133747234
56-57	4.971184528878357E-5	0.0	0.0	0.9011514754724241	0.0014416435133747234
58-59	4.971184528878357E-5	0.0	0.0	1.019341387646507	0.0014664994360191154
60-61	4.971184528878357E-5	0.0	0.0	1.1437949923269768	0.0015162112813078988
62-63	4.971184528878357E-5	0.0	0.0	1.2800800161861767	0.0015907790492410742
64-65	4.971184528878357E-5	0.0	0.0	1.4034399602702932	0.0016404908945298576
66-67	4.971184528878357E-5	0.0	0.0	1.543627363984663	0.0017399145851074247
68-69	4.971184528878357E-5	0.0	0.0	1.7004185240254863	0.0017896264303962084
70-71	4.971184528878357E-5	0.0	0.0	1.8694387980073506	0.0017896264303962084
72-73	4.971184528878357E-5	0.0	0.0	2.0239929250101785	0.0019884738115513428
74-75	4.971184528878357E-5	0.0	0.0	2.186302099878057	0.0020878975021289096
76-77	4.971184528878357E-5	0.0	0.0	2.378413525996561	0.0021376093474176935
78-79	4.971184528878357E-5	0.0	0.0	2.5933426891026166	0.002187321192706477
80-81	4.971184528878357E-5	0.0	0.0	2.815430357930257	0.0022121771153508687
82-83	4.971184528878357E-5	0.0	0.0	3.040003619022337	0.002411024496506003
84-85	4.971184528878357E-5	0.0	0.0	3.2729036142002883	0.0024358804191503946
86-87	4.971184528878357E-5	0.0	0.0	3.522133950555604	0.00251044818708357
88-89	4.971184528878357E-5	0.0	0.0	3.8072065273641336	0.0025850159550167453
90-91	4.971184528878357E-5	0.0	0.0	4.116637908364167	0.0025850159550167453
92-93	4.971184528878357E-5	0.0	0.0	4.416375479532888	0.002634727800305529
94-95	4.971184528878357E-5	0.0	0.0	4.721755345141885	0.0027838633361718795
96-97	4.971184528878357E-5	0.0	0.0	5.055794089559866	0.0028087192588162717
98-99	4.971184528878357E-5	0.0	0.0	5.438103035753256	0.0028335751814606634
100-101	4.971184528878357E-5	0.0	0.0	5.847281234325234	0.0028335751814606634
102-103	4.971184528878357E-5	0.0	0.0	6.263269955701775	0.0028335751814606634
104-105	4.971184528878357E-5	0.0	0.0	6.697478068376655	0.002883287026749447
106-107	4.971184528878357E-5	0.0	0.0	7.162209254058848	0.0029329988720382303
108-109	4.971184528878357E-5	0.0	0.0	7.640810044576611	0.0030324225626157976
110-111	4.971184528878357E-5	0.0	0.0	8.165046309069478	0.0030572784852601893
112-113	4.971184528878357E-5	0.0	0.0	8.695198283151711	0.003082134407904581
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2750	0.0	56.261868	1
GGTATCA	1575	0.0	55.918392	1
TCAACGC	3495	0.0	43.074997	4
CAACGCA	3660	0.0	40.96949	5
GTACATG	10100	0.0	40.005703	1
AACGCAG	3865	0.0	38.948483	6
TATCAAC	3920	0.0	38.404877	2
TACATGG	10620	0.0	37.596718	2
ACATGGG	10570	0.0	37.2679	3
ATCAACG	4150	0.0	36.419796	3
CATGGGG	9630	0.0	34.973736	4
ATGGGGG	5995	0.0	33.647408	5
ACGCAGA	4975	0.0	30.014793	7
CGCAGAG	5360	0.0	27.747887	8
TGGGGGG	4110	0.0	26.637625	6
GTATACG	120	0.0012872216	24.794893	1
GAGTACA	7060	0.0	24.359402	1
GTATAGG	575	0.0	23.803097	1
AGTACAT	6965	0.0	23.66522	2
GCAGAGT	6405	0.0	23.03495	9
>>END_MODULE