FastQCFastQC Report
Mon 15 Aug 2016
SRR2921320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011593
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.071.238691
GTATCAA36250.044.8125841
GTACATG94600.039.520491
TACATGG99300.037.267382
ACATGGG98000.037.0320473
TCAACGC47300.034.2684365
CATGGGG92150.033.3041534
CAACGCA49150.033.0882846
AACGCAG52000.031.2662337
TATCAAC51800.031.1764073
ATCAACG52850.030.6697644
ATGGGGG57400.030.626455
ACGCAGA65400.024.6748098
GTGGTAT10650.023.5525991
CGCAGAG69550.023.2880979
TGGGGGG40800.023.2151836
CCTATAC4950.022.8736213
TGGTATC11350.022.0515922
TATCCGT1903.276903E-521.9472266
TATACAG7700.021.6697465
Produced by FastQC (version 0.11.5)