##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921320_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2011593
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.385929459885773	33.0	30.0	34.0	22.0	34.0
2	30.127850912187505	33.0	31.0	34.0	26.0	34.0
3	30.363329460780584	33.0	31.0	34.0	27.0	34.0
4	32.57519985404602	37.0	35.0	37.0	19.0	37.0
5	32.23734821109439	37.0	35.0	37.0	19.0	37.0
6	32.39238603435188	37.0	35.0	37.0	17.0	37.0
7	32.59674645914954	37.0	35.0	37.0	17.0	37.0
8	32.852501475199006	37.0	35.0	37.0	17.0	37.0
9	34.61721779703946	39.0	37.0	39.0	19.0	39.0
10-11	34.79312564718609	39.0	37.0	39.0	26.0	39.0
12-13	34.819246239174625	39.0	37.0	39.0	26.5	39.0
14-15	36.21637577780396	41.0	38.0	41.0	27.0	41.0
16-17	36.24454673485143	41.0	38.0	41.0	27.0	41.0
18-19	36.26488981618051	41.0	38.0	41.0	27.0	41.0
20-21	36.262863561366544	41.0	38.0	41.0	27.0	41.0
22-23	36.21442036236952	41.0	38.0	41.0	27.0	41.0
24-25	36.15756069940589	41.0	38.0	41.0	27.0	41.0
26-27	36.07091866992975	41.0	38.0	41.0	26.0	41.0
28-29	36.00498684376014	40.0	38.0	41.0	25.0	41.0
30-31	35.92647344666639	40.0	38.0	41.0	25.0	41.0
32-33	35.81769771519387	40.0	37.0	41.0	24.5	41.0
34-35	35.74044650185201	40.0	37.0	41.0	24.0	41.0
36-37	35.66352711507746	40.0	37.0	41.0	24.0	41.0
38-39	35.57347236742224	40.0	37.0	41.0	23.5	41.0
40-41	35.4518990670578	40.0	36.5	41.0	22.5	41.0
42-43	35.31158042407186	40.0	36.0	41.0	20.5	41.0
44-45	35.17175293411739	40.0	36.0	41.0	18.5	41.0
46-47	34.98093401597639	40.0	35.0	41.0	16.0	41.0
48-49	34.78824593245254	40.0	35.0	41.0	13.0	41.0
50-51	34.61110671989811	40.0	35.0	41.0	10.0	41.0
52-53	34.4227634516525	39.0	35.0	41.0	8.5	41.0
54-55	34.21582646191352	39.0	35.0	41.0	7.5	41.0
56-57	33.96674774668633	39.0	35.0	41.0	6.5	41.0
58-59	33.89199107374106	38.5	35.0	41.0	2.0	41.0
60-61	33.64707100293151	38.0	35.0	41.0	2.0	41.0
62-63	33.35600591173264	37.0	34.5	40.0	2.0	41.0
64-65	33.04811584649579	37.0	34.0	40.0	2.0	41.0
66-67	32.72942140880387	36.0	34.0	39.0	2.0	41.0
68-69	32.38651108847565	35.5	34.0	39.0	2.0	41.0
70-71	32.05486273813838	35.0	34.0	38.5	2.0	40.5
72-73	31.713187260047135	35.0	33.5	37.0	2.0	39.5
74-75	31.38364967466083	35.0	33.0	37.0	2.0	39.0
76-77	31.091728794045316	35.0	33.0	36.0	2.0	39.0
78-79	30.84173115535797	35.0	33.0	36.0	2.0	37.0
80-81	30.619601231461832	35.0	33.0	35.5	2.0	37.0
82-83	30.428024704798634	35.0	33.0	35.0	2.0	36.5
84-85	30.26354138237705	35.0	33.0	35.0	2.0	36.0
86-87	30.128962220488937	35.0	33.0	35.0	2.0	36.0
88-89	30.014879252413387	35.0	32.5	35.0	2.0	36.0
90-91	29.922217118472773	35.0	32.0	35.0	2.0	35.0
92-93	29.858153712008345	35.0	32.0	35.0	2.0	35.0
94-95	29.792748085721115	35.0	32.0	35.0	2.0	35.0
96-97	29.728587492599146	35.0	32.0	35.0	2.0	35.0
98-99	29.674787096594592	35.0	32.0	35.0	2.0	35.0
100-101	29.621576283075154	35.0	32.0	35.0	2.0	35.0
102-103	28.94380846423705	34.0	30.5	35.0	2.0	35.0
104-105	29.49484861003195	35.0	32.0	35.0	2.0	35.0
106-107	29.558325913840427	35.0	32.0	35.0	2.0	35.0
108-109	29.631638706239283	35.0	32.0	35.0	2.0	35.0
110-111	29.743874382143904	35.0	33.0	35.0	2.0	35.0
112-113	29.707694598261178	35.0	33.0	35.0	2.0	35.0
114-115	29.6471169863884	35.0	33.0	35.0	2.0	35.0
116-117	29.585756661511546	35.0	33.0	35.0	2.0	35.0
118-119	29.50896503417938	35.0	33.0	35.0	2.0	35.0
120-121	29.412529274062894	35.0	32.0	35.0	2.0	35.0
122-123	29.296517983508593	35.0	32.0	35.0	2.0	35.0
124-125	28.77176844421312	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	129718.0
3	7252.0
4	5783.0
5	5507.0
6	5549.0
7	5948.0
8	6288.0
9	6269.0
10	6454.0
11	6350.0
12	7039.0
13	7812.0
14	9044.0
15	7810.0
16	5527.0
17	4957.0
18	4522.0
19	4163.0
20	4133.0
21	4117.0
22	4961.0
23	6256.0
24	8101.0
25	10265.0
26	12471.0
27	14416.0
28	17587.0
29	20867.0
30	25403.0
31	31628.0
32	41338.0
33	55489.0
34	85342.0
35	146887.0
36	341326.0
37	710447.0
38	234308.0
39	259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.68607013250442	17.262572562300612	11.497129419930411	27.554227885264552
2	16.13919913223003	19.02323183665881	37.50694101639845	27.330628014712715
3	19.22660299573522	22.51151202057275	28.179557196709276	30.082327786982756
4	13.647311169295055	15.360925515824519	31.0974888203631	39.894274494517326
5	14.830890356323772	37.212337340950896	32.10503987866608	15.851732424059248
6	35.522608072045045	33.23947080232074	15.81530599151955	15.42261513411467
7	30.911689127909753	31.286744816942196	18.373900141609255	19.4276659135388
8	28.782599876506016	34.8533434355788	17.35219748696701	19.011859200948177
9	28.057651598687922	15.524777500146659	17.536548043164622	38.8810228580008
10-11	27.44333821681984	25.21531999596329	25.096779036569476	22.24456275064739
12-13	27.083385144605675	22.34026626185898	25.918327860405014	24.658020733130332
14-15	24.692196681933172	23.70076849541632	22.99269782704553	28.614336995604976
16-17	24.968171991053858	26.727001933293664	23.741557064475767	24.563269011176715
18-19	25.129213513866873	25.76450604073488	25.292566637485812	23.813713807912436
20-21	25.60609924572217	24.74359872996178	25.241686563832744	24.408615460483308
22-23	25.517139898577895	24.365366155082064	25.042391776070012	25.075102170270032
24-25	25.116164154478565	24.840163989435236	24.985546280982284	25.05812557510391
26-27	24.835739635204536	24.888036496448336	25.234726905492316	25.041496962854815
28-29	24.829177671626418	25.212679702106737	25.086560750609095	24.87158187565775
30-31	25.28568154691332	24.999788724657524	25.04982369694068	24.664706031488475
32-33	24.890099538027822	25.041770378003903	25.11874917043358	24.949380913534696
34-35	25.081639277925504	25.13910617107934	25.351052623468068	24.428201927527088
36-37	25.168461015722364	25.100430355444665	25.27852304119173	24.452585587641238
38-39	25.04480280056652	25.309717224110447	25.24223339413092	24.40324658119212
40-41	24.868276037946046	25.315409230396007	25.202091079060228	24.614223652597715
42-43	24.78142944422654	25.276435143689604	25.082061828610456	24.860073583473397
44-45	24.646188369118406	25.301241354488706	25.286800063432313	24.765770212960575
46-47	24.688816276453537	25.304820607349498	24.94155129790171	25.06481181829525
48-49	24.623097216981765	25.4499543396701	24.91351381715884	25.013434626189294
50-51	24.564685798767442	25.52260820155966	25.092749875347547	24.819956124325348
52-53	24.821397767838725	25.445952536124356	25.058821540937952	24.67382815509897
54-55	24.426983987317513	25.14765660846901	25.31207853676166	25.113280867451813
56-57	24.56369156186167	25.28943479123262	25.014851413780026	25.13202223312569
58-59	24.61887171013222	25.386049762551373	25.114374528048167	24.880703999268242
60-61	24.932727445362953	25.19856153804472	25.18218148502207	24.686529531570255
62-63	24.67246107935353	25.272010789458903	25.262316979627585	24.793211151559984
64-65	24.51072359070647	24.970930998467384	25.276012593004648	25.2423328178215
66-67	24.5688615937717	25.39452563217311	24.991983964947185	25.044628809108005
68-69	24.441748405368283	25.268481248443397	25.113653706291483	25.17611663989684
70-71	24.395267830023272	25.14522072804986	25.2430039277329	25.216507514193975
72-73	24.504708457426528	25.44712076448864	24.87148245196717	25.176688326117656
74-75	24.290375836463937	25.472374381895346	25.016094209912243	25.221155571728477
76-77	24.372574372648938	25.102915947709104	25.05444689855254	25.470062781089414
78-79	24.248717310111935	25.141417771885266	25.234801473260248	25.37506344474255
80-81	24.43108521455384	25.419282131126923	24.829277095316996	25.320355559002245
82-83	24.461856846787597	25.40546223813664	24.92884992143043	25.203830993645337
84-85	24.45865043276647	25.480651404135923	24.784064172026845	25.276633991070756
86-87	24.099755765704096	25.581019619773983	24.95974583327741	25.359478781244515
88-89	24.130353406479344	25.36062215368616	24.96491586518744	25.544108574647055
90-91	24.295829961204777	25.177173456990197	25.023339769376236	25.503656812428787
92-93	24.254884644418368	25.423664777691418	25.041602776318868	25.279847801571346
94-95	24.308556912690957	25.2675744063154	24.8953066976869	25.528561983306737
96-97	24.0672156533281	25.255010352532953	24.977070320170412	25.70070367396854
98-99	23.93205774236854	25.31230884218234	24.901937049314956	25.85369636613416
100-101	23.974556483344294	25.31130800315968	25.011446152377744	25.70268936111828
102-103	23.765269614678516	25.283767640869698	25.0501716798577	25.90079106459408
104-105	23.814334027145676	25.299974000679065	25.03702293358839	25.84866903858687
106-107	23.90727635669387	25.187308251853445	25.06384873064374	25.841566660808944
108-109	23.81672850847889	25.224947106359963	25.1094659730839	25.84885841207725
110-111	23.710828735379053	25.583706961134588	24.942321544773442	25.763142758712913
112-113	23.663424268621085	25.6394112678759	24.99777537722891	25.699389086274106
114-115	23.652668266398617	25.620045267741343	24.902253792652267	25.825032673207772
116-117	23.408706147933078	25.45486044914479	25.044759625339008	26.091673777583125
118-119	23.51082093660899	25.426870780848848	25.009861619174718	26.052446663367444
120-121	23.419185818183628	25.514170382632646	25.122142186031944	25.94450161315179
122-123	23.366067495940957	25.702142319535405	24.83152362497129	26.10026655955235
124-125	23.182283666154635	25.57039456350449	24.80547725928246	26.441844511058417
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	33.0
2	50.5
3	60.5
4	52.0
5	72.5
6	145.0
7	359.0
8	552.0
9	613.5
10	587.0
11	521.5
12	541.0
13	602.0
14	666.5
15	718.5
16	736.0
17	751.0
18	826.5
19	902.5
20	921.0
21	998.5
22	1125.0
23	1278.0
24	1474.0
25	1688.5
26	2074.0
27	2599.0
28	3266.5
29	4220.0
30	5658.5
31	7406.5
32	9687.0
33	12432.0
34	15095.0
35	18129.0
36	22332.0
37	26865.5
38	30773.0
39	35207.5
40	39760.5
41	43542.5
42	48143.5
43	54757.0
44	61405.0
45	68321.0
46	73911.0
47	78776.5
48	86123.0
49	91620.0
50	94273.5
51	96214.5
52	96413.0
53	95318.5
54	95577.5
55	94158.5
56	88099.0
57	80628.0
58	74287.0
59	67462.0
60	58142.0
61	48392.5
62	39878.5
63	31129.5
64	22740.0
65	16858.5
66	12714.0
67	9627.0
68	7336.0
69	5489.0
70	4209.5
71	3147.0
72	2353.0
73	1889.5
74	1415.5
75	988.5
76	736.5
77	536.5
78	394.5
79	313.5
80	269.0
81	241.5
82	203.5
83	168.5
84	153.5
85	137.0
86	132.5
87	137.0
88	121.0
89	119.0
90	117.5
91	112.5
92	111.5
93	96.5
94	82.5
95	82.5
96	69.5
97	55.5
98	45.0
99	18.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30199946012936013
2	0.0
3	0.0
4	0.017250010315207898
5	0.16161320903383536
6	0.04230478034075481
7	0.021127534247733015
8	0.007904183400916587
9	0.006512251732830647
10-11	0.0025850159550167453
12-13	0.05423562321006287
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.4855922644391785E-4
92-93	4.225506849546603E-4
94-95	1.491355358663507E-4
96-97	3.9769476231026854E-4
98-99	1.2427961322195892E-4
100-101	0.0
102-103	0.0
104-105	9.942369057756714E-5
106-107	1.2427961322195892E-4
108-109	4.474066075990521E-4
110-111	4.971184528878357E-4
112-113	7.953895246205371E-4
114-115	2.982710717327014E-4
116-117	7.208217566873617E-4
118-119	3.2312699437709317E-4
120-121	1.491355358663507E-4
122-123	0.0017399145851074247
124-125	1.491355358663507E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2011593.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.0679247384582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43449931991579	48.91601376262727
2	9.638363015484513	11.579129284321256
3	3.327563110291306	5.996394314143442
4	1.5971183123180792	3.83742330330943
5	0.9530111211493151	2.862270015005536
6	0.5993386032496093	2.1600615667710117
7	0.44710857056752135	1.8799818776738653
8	0.31819275009589126	1.529054253206643
9	0.2400700981057065	1.297846132647107
>10	1.3535368287527836	14.480716086886405
>50	0.06675619258172708	2.7290501963556144
>100	0.02320017287935038	2.1169686155387075
>500	9.935236866077332E-4	0.40904916843471767
>1k	2.483809216519309E-4	0.20604142307903014
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.971184528878357E-5
7	0.0	0.0	0.0	0.0	4.971184528878357E-5
8	0.0	0.0	0.0	0.0	4.971184528878357E-5
9	0.0	0.0	0.0	0.0	9.942369057756714E-5
10-11	0.0	0.0	0.0	0.0	9.942369057756714E-5
12-13	0.0	0.0	0.0	0.0	9.942369057756714E-5
14-15	0.0	0.0	0.0	4.971184528878357E-5	1.739914585107425E-4
16-17	0.0	0.0	0.0	9.942369057756714E-5	2.4855922644391785E-4
18-19	0.0	0.0	0.0	1.2427961322195892E-4	2.4855922644391785E-4
20-21	0.0	0.0	0.0	1.491355358663507E-4	2.734151490883096E-4
22-23	0.0	0.0	0.0	1.9884738115513427E-4	3.2312699437709317E-4
24-25	0.0	0.0	0.0	5.219743755322274E-4	3.4798291702148494E-4
26-27	0.0	0.0	0.0	9.942369057756714E-4	3.7283883966587677E-4
28-29	0.0	0.0	0.0	0.00347982917021485	3.9769476231026854E-4
30-31	0.0	0.0	0.0	0.011881131024019272	3.9769476231026854E-4
32-33	0.0	0.0	0.0	0.029653115714759398	4.971184528878357E-4
34-35	0.0	0.0	0.0	0.04981126897936113	4.971184528878357E-4
36-37	0.0	0.0	0.0	0.08311820532284613	4.971184528878357E-4
38-39	0.0	0.0	0.0	0.14108221692956774	4.971184528878357E-4
40-41	0.0	0.0	0.0	0.2066521408654733	4.971184528878357E-4
42-43	0.0	0.0	0.0	0.26488956762128324	4.971184528878357E-4
44-45	0.0	0.0	0.0	0.3327462364404728	4.971184528878357E-4
46-47	0.0	0.0	0.0	0.40788569059446916	5.468302981766192E-4
48-49	0.0	0.0	0.0	0.4906310570776494	5.965421434654028E-4
50-51	0.0	0.0	0.0	0.5727053136494311	6.959658340429699E-4
52-53	0.0	0.0	0.0	0.6566934762648309	7.705336019761453E-4
54-55	0.0	0.0	0.0	0.7480638479056152	8.451013699093206E-4
56-57	0.0	0.0	0.0	0.8477858095549149	8.451013699093206E-4
58-59	0.0	0.0	0.0	0.9580466824054369	8.451013699093206E-4
60-61	0.0	0.0	0.0	1.076186882734231	8.451013699093206E-4
62-63	0.0	0.0	0.0	1.2047417146510253	8.948132151981042E-4
64-65	0.0	0.0	0.0	1.3219125339966882	8.948132151981042E-4
66-67	0.0	0.0	0.0	1.45322637332701	9.19669137842496E-4
68-69	0.0	0.0	0.0	1.6016410874366733	9.445250604868877E-4
70-71	0.0	0.0	0.0	1.7601970179852486	9.445250604868877E-4
72-73	0.0	0.0	0.0	1.904063098250988	0.0010439487510644548
74-75	0.0	0.0	0.0	2.055162252006246	0.0010688046737088465
76-77	0.0	0.0	0.0	2.237207029453771	0.0010936605963532385
78-79	0.0	0.0	0.0	2.43913654501681	0.0011185165189976302
80-81	0.0	0.0	0.0	2.6478268715391238	0.001143372441642022
82-83	0.0	0.0	0.0	2.858704519254143	0.0012427961322195892
84-85	0.0	0.0	0.0	3.0772129352209916	0.0012427961322195892
86-87	0.0	0.0	0.0	3.3104857692386087	0.0012427961322195892
88-89	0.0	0.0	0.0	3.577811217279042	0.0012427961322195892
90-91	0.0	0.0	0.0	3.869768884660068	0.0012427961322195892
92-93	0.0	0.0	0.0	4.15225644551358	0.0012925079775083727
94-95	0.0	0.0	0.0	4.440833707414969	0.0012925079775083727
96-97	0.0	0.0	0.0	4.756106230236435	0.0014664994360191151
98-99	0.0	0.0	0.0	5.116989371110359	0.0015410672039522905
100-101	0.0	0.0	0.0	5.50315098531363	0.0015410672039522905
102-103	0.0	0.0	0.0	5.895725427559153	0.0016404908945298576
104-105	0.0	0.0	0.0	6.306320413721862	0.0016404908945298576
106-107	0.0	0.0	0.0	6.746369668218174	0.0016653468171742495
108-109	0.0	0.0	0.0	7.199890832787746	0.0016902027398186413
110-111	0.0	0.0	0.0	7.695095379631963	0.0017647705077518164
112-113	2.4855922644391784E-5	0.0	0.0	8.196663042673144	0.0017896264303962084
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2230	0.0	71.23869	1
GTATCAA	3625	0.0	44.812584	1
GTACATG	9460	0.0	39.52049	1
TACATGG	9930	0.0	37.26738	2
ACATGGG	9800	0.0	37.032047	3
TCAACGC	4730	0.0	34.268436	5
CATGGGG	9215	0.0	33.304153	4
CAACGCA	4915	0.0	33.088284	6
AACGCAG	5200	0.0	31.266233	7
TATCAAC	5180	0.0	31.176407	3
ATCAACG	5285	0.0	30.669764	4
ATGGGGG	5740	0.0	30.62645	5
ACGCAGA	6540	0.0	24.674809	8
GTGGTAT	1065	0.0	23.552599	1
CGCAGAG	6955	0.0	23.288097	9
TGGGGGG	4080	0.0	23.215183	6
CCTATAC	495	0.0	22.873621	3
TGGTATC	1135	0.0	22.051592	2
TATCCGT	190	3.276903E-5	21.947226	6
TATACAG	770	0.0	21.669746	5
>>END_MODULE