FastQCFastQC Report
Mon 15 Aug 2016
SRR2921319_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921319_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613864
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14400.059.0951461
GTATCAA24350.053.765291
TCAACGC29200.044.2209664
CAACGCA30300.042.0238235
TAGTACG1900.040.713752
GTACATG81950.039.9385221
AACGCAG32700.039.120266
TATCAAC33100.039.010642
TACATGG84450.038.260582
ATCAACG34100.037.692133
ACATGGG87000.036.6603743
CATGGGG79400.035.8976754
ATGGGGG46750.033.600585
GTACGGT1301.7355796E-632.0409974
ACGCAGA41300.030.5391127
CGCAGAG45100.028.0978878
GAGTACA57450.026.3100661
AGTACAT56900.025.9352722
TACGGTC1651.1100456E-525.2428575
TGGGGGG30250.025.176516
Produced by FastQC (version 0.11.5)