##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921319_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1613864
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.653573039611764	31.0	31.0	34.0	27.0	34.0
2	30.751303083779053	33.0	31.0	34.0	27.0	34.0
3	30.7808123856781	33.0	31.0	34.0	27.0	34.0
4	34.24831646284941	37.0	35.0	37.0	31.0	37.0
5	34.327199813615024	37.0	35.0	37.0	31.0	37.0
6	34.28373208647073	37.0	35.0	37.0	31.0	37.0
7	34.31259263481929	37.0	35.0	37.0	31.0	37.0
8	34.31035886543104	37.0	35.0	37.0	31.0	37.0
9	35.924317662454825	39.0	37.0	39.0	31.0	39.0
10-11	35.95807794213143	39.0	37.0	39.0	31.5	39.0
12-13	35.893065338838966	39.0	37.0	39.0	31.0	39.0
14-15	37.23279687755598	41.0	38.0	41.0	31.0	41.0
16-17	37.213363703509096	41.0	38.0	41.0	31.0	41.0
18-19	37.189114138489984	41.0	38.0	41.0	31.0	41.0
20-21	37.22036646210585	41.0	38.0	41.0	31.0	41.0
22-23	37.157564082227495	41.0	38.0	41.0	31.0	41.0
24-25	37.13103086753283	41.0	38.0	41.0	31.0	41.0
26-27	37.10118541587147	41.0	38.0	41.0	30.0	41.0
28-29	37.03313631136204	40.0	38.0	41.0	30.0	41.0
30-31	36.95782699161764	40.0	38.0	41.0	30.0	41.0
32-33	36.8843651013964	40.0	38.0	41.0	30.0	41.0
34-35	36.77019748875989	40.0	38.0	41.0	30.0	41.0
36-37	36.66047975541929	40.0	38.0	41.0	30.0	41.0
38-39	36.56521986982794	40.0	37.0	41.0	30.0	41.0
40-41	36.45949163002582	40.0	37.0	41.0	29.5	41.0
42-43	36.256571185676115	40.0	37.0	41.0	28.5	41.0
44-45	35.99065348753055	40.0	36.0	41.0	28.0	41.0
46-47	35.76914690457188	40.0	36.0	41.0	27.0	41.0
48-49	35.54967054225139	40.0	35.0	41.0	27.0	41.0
50-51	35.36516986561445	39.0	35.0	41.0	26.0	41.0
52-53	35.16607966966238	39.0	35.0	41.0	26.0	41.0
54-55	34.94863693594999	39.0	35.0	41.0	26.0	41.0
56-57	34.6877301928787	38.5	35.0	40.0	25.5	41.0
58-59	34.401448015446164	38.0	34.0	40.0	25.0	41.0
60-61	34.09565954752074	37.5	34.0	40.0	24.0	41.0
62-63	33.76283999147388	37.0	34.0	40.0	23.0	41.0
64-65	33.40134050948531	36.0	33.5	39.0	22.5	41.0
66-67	33.05356647152424	36.0	33.0	39.0	22.0	40.5
68-69	32.695435612914096	35.0	33.0	38.5	21.5	40.0
70-71	32.63781675531519	35.0	33.0	38.0	22.0	40.0
72-73	32.3494969216737	35.0	33.0	37.0	21.5	39.0
74-75	32.028551972161225	35.0	33.0	37.0	20.5	39.0
76-77	31.73883270213599	35.0	33.0	36.0	20.0	39.0
78-79	31.464621864048024	35.0	33.0	36.0	20.0	37.0
80-81	31.195720333311854	35.0	33.0	35.0	18.5	37.0
82-83	30.984610537195202	35.0	32.0	35.0	18.0	36.5
84-85	30.781199964804966	35.0	32.0	35.0	17.5	36.0
86-87	30.624520095869293	35.0	32.0	35.0	15.0	36.0
88-89	30.48602019748876	35.0	32.0	35.0	12.0	35.5
90-91	30.365586877208983	35.0	32.0	35.0	10.0	35.0
92-93	30.25320628008308	35.0	32.0	35.0	7.0	35.0
94-95	30.133541302117155	35.0	32.0	35.0	7.0	35.0
96-97	30.033897527920566	35.0	31.5	35.0	3.5	35.0
98-99	29.946403166561744	35.0	31.0	35.0	2.0	35.0
100-101	29.850121199803702	35.0	31.0	35.0	2.0	35.0
102-103	28.97418121973103	33.5	29.5	34.5	2.0	35.0
104-105	29.581028822750866	34.0	31.0	35.0	2.0	35.0
106-107	29.6573496899367	34.0	31.0	35.0	2.0	35.0
108-109	29.586945058567515	34.0	31.0	35.0	2.0	35.0
110-111	29.488661684008072	34.0	31.0	35.0	2.0	35.0
112-113	29.388764170958645	34.0	31.0	35.0	2.0	35.0
114-115	29.255857680696764	34.0	31.0	35.0	2.0	35.0
116-117	29.121186791452068	34.0	30.5	35.0	2.0	35.0
118-119	29.11584154550817	34.0	30.5	35.0	2.0	35.0
120-121	29.278279334565987	35.0	31.0	35.0	2.0	35.0
122-123	29.1360631998731	35.0	31.0	35.0	2.0	35.0
124-125	28.54140001883678	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53531.0
3	7302.0
4	4768.0
5	3988.0
6	4313.0
7	4826.0
8	5148.0
9	5557.0
10	5817.0
11	6218.0
12	6016.0
13	6049.0
14	6106.0
15	5651.0
16	4758.0
17	4433.0
18	4351.0
19	4292.0
20	4312.0
21	4562.0
22	5257.0
23	6571.0
24	9184.0
25	11887.0
26	13180.0
27	14896.0
28	17507.0
29	21382.0
30	26460.0
31	33301.0
32	43185.0
33	58791.0
34	90228.0
35	150893.0
36	322419.0
37	515271.0
38	121439.0
39	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.05836720073672	16.72962967094394	11.608060256090864	25.60394287222848
2	16.684503577193954	18.479824254273577	39.02156231505954	25.814109853472928
3	20.502856165512593	22.699916093553828	27.722528694889142	29.07469904604444
4	14.050279208437418	13.875274297269142	33.891584953295876	38.182861540997564
5	14.910821733941281	37.17688293113983	32.86142573683147	15.050869598087417
6	35.82612015504356	33.21412944179111	16.142417454920476	14.817332948244852
7	31.3749002285448	31.034809702990913	18.604153962847835	18.986136105616453
8	28.738401589895464	34.76960582912094	18.033811925579307	18.458180655404288
9	28.111117376698914	15.488615667949185	17.607217461408283	38.79304949394362
10-11	27.462217096852754	24.790721538965855	25.83728425372066	21.90977711046073
12-13	26.938085912144587	21.924349499450514	26.436498486624387	24.701066101780516
14-15	24.341731834574727	23.414374945779475	23.3821524619217	28.861740757724103
16-17	25.13706779030859	26.554033957119845	23.957367703556823	24.351530549014747
18-19	25.052423372644668	26.046174704917142	25.117580791481387	23.783821130956806
20-21	25.699828168366494	24.8960056116382	25.13596882863869	24.268197391356612
22-23	25.767278126198654	24.488920174818393	24.979876551395506	24.763925147587447
24-25	25.140662663667516	24.80090346327589	25.03240818198155	25.02602569107505
26-27	24.951395819126454	24.974477963987514	25.166447050644088	24.907679166241945
28-29	25.019627745173313	25.385847078951556	25.008752704043346	24.585772471831785
30-31	25.446209339080063	25.0635693586861	24.890095573242714	24.60012572899113
32-33	25.085042260093143	25.096072424805104	25.0089465170241	24.809938798077653
34-35	25.152849603673904	25.23371667215592	25.14814010390024	24.465293620269936
36-37	25.150826758674704	25.204551820207566	25.05747404218192	24.58714737893581
38-39	25.20710077504969	25.399538657210535	24.856957399377542	24.536403168362234
40-41	25.22905265414792	25.127798848222465	24.997823414973606	24.64532508265601
42-43	24.9475763025058	25.03036360729569	24.997180522179686	25.02487956801882
44-45	24.756408905853995	25.151105795745366	25.19346003457742	24.899025263823223
46-47	24.871091457675053	24.979906936738917	25.156979961468412	24.992021644117614
48-49	24.789876520794532	25.25453856490474	25.03328420356641	24.922300710734323
50-51	24.774699271124607	25.289079262294443	25.13026033477146	24.805961131809482
52-53	24.86393839103081	25.196509337330546	25.095659846907065	24.843892424731578
54-55	24.632059740448053	24.89207023753533	25.32750726399679	25.148362758019832
56-57	24.765129991045782	25.127853188041627	25.086552255130083	25.0204645657825
58-59	24.860281018109713	25.22811420427259	25.134141996251024	24.777462781366673
60-61	25.117742613608723	25.091747994092188	25.211093970704468	24.579415421594618
62-63	24.975438246437964	25.152382017605145	25.093576330742017	24.77860340521487
64-65	24.842182621700243	24.98591068411645	25.079787819085436	25.092118875097867
66-67	24.815429072992153	25.155493783192018	25.030200262050595	24.998876881765238
68-69	24.621989752004456	25.04751270155331	25.14672083352181	25.18377671292042
70-71	24.62164552863665	24.940613676746928	25.3150410754757	25.122699719140723
72-73	24.683302521031834	25.28916934434707	24.955609527430074	25.071918607191023
74-75	24.4576643923344	25.248991979834017	25.15294579835135	25.140397829480225
76-77	24.563080927004584	24.875913991820546	25.18100136324204	25.38000371793283
78-79	24.501748801523345	24.891042647582335	25.31962217583813	25.287586375056193
80-81	24.687736530960827	25.140574716596596	25.039448290904538	25.13224046153803
82-83	24.74222236543202	25.130592876166354	25.192000961708867	24.935183796692762
84-85	24.751261354045443	25.205702026718026	24.920526329487615	25.122510289748917
86-87	24.408605681970986	25.302660662510355	25.157317859845048	25.13141579567361
88-89	24.402714090965425	25.001146362622386	25.26223819556327	25.333901350848926
90-91	24.671666276798287	24.913240085079092	25.21820494270484	25.19688869541778
92-93	24.629157991275036	25.008799325798137	25.255428316478284	25.106614366448543
94-95	24.68390061176529	24.90667054990713	25.20609317608275	25.203335662244836
96-97	24.379944433651755	24.813159935590875	25.379853651115585	25.427041979641785
98-99	24.332009834069442	24.930667331772813	25.216518386208758	25.52080444794899
100-101	24.39667942468396	24.962626021955607	25.26651325649192	25.37418129686852
102-103	24.151005829747575	24.909405920961312	25.428431916876526	25.511156332414586
104-105	24.23025551207291	25.025429135050008	25.27834175084436	25.465973602032722
106-107	24.283262174907204	24.907173884754332	25.376478680357177	25.433085259981286
108-109	24.218447913465067	24.880001933353824	25.447986585498857	25.45356356768226
110-111	24.12986893077092	25.200222708937574	25.359167735337014	25.31074062495449
112-113	24.041513353005403	25.27364087361832	25.336474172044266	25.348371601332016
114-115	24.135913985957007	25.280489667588803	25.28293726195983	25.300659084494363
116-117	23.912153744579243	25.226613013413214	25.35788543886104	25.503347803146507
118-119	23.991883006588466	25.095048305169666	25.379161688684576	25.533906999557292
120-121	23.88283111961044	25.111016643511086	25.52230185868446	25.483850378194013
122-123	23.787631259587027	25.28161964563281	25.38841498307292	25.542334111707245
124-125	23.779272140868766	25.182847768601956	25.33896585323537	25.698914237293913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	239.0
1	207.5
2	166.5
3	146.0
4	114.5
5	102.5
6	120.5
7	138.5
8	171.5
9	222.0
10	235.0
11	237.0
12	261.0
13	272.5
14	288.0
15	328.5
16	354.0
17	358.0
18	387.5
19	431.0
20	455.0
21	494.0
22	560.5
23	659.5
24	800.0
25	970.0
26	1191.0
27	1590.5
28	2116.0
29	2873.5
30	4153.5
31	5612.0
32	7451.5
33	9619.5
34	11943.0
35	14524.5
36	17776.5
37	21331.5
38	24593.0
39	28425.5
40	32423.5
41	36098.0
42	40011.0
43	44764.5
44	49908.5
45	54755.5
46	59313.0
47	63793.0
48	69190.0
49	73431.5
50	75677.5
51	77551.5
52	78255.5
53	77676.0
54	77193.5
55	76116.5
56	72719.5
57	66720.5
58	60744.0
59	54760.5
60	46925.5
61	39070.5
62	31643.5
63	24263.5
64	17688.0
65	13098.0
66	9833.5
67	7310.0
68	5491.0
69	4069.5
70	3084.5
71	2321.5
72	1672.5
73	1228.5
74	949.5
75	704.0
76	503.0
77	334.5
78	206.0
79	130.5
80	89.5
81	63.0
82	43.5
83	41.5
84	37.5
85	31.5
86	26.5
87	15.5
88	10.5
89	13.0
90	13.0
91	9.5
92	8.0
93	7.0
94	6.0
95	4.5
96	2.5
97	1.5
98	2.5
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013507953582210151
2	0.010471762180704197
3	0.010038020551917635
4	0.012082802516197153
5	0.008055201677464768
6	0.023669900313781085
7	0.010905503809490763
8	0.010719614540010806
9	0.006877902970758379
10-11	0.006506124431798466
12-13	0.005421770359832055
14-15	0.005204899545438773
16-17	0.003965637748905732
18-19	0.004957047186132165
20-21	0.0046472317369989045
22-23	0.0046472317369989045
24-25	0.004585268647172252
26-27	0.003872693114165753
28-29	0.004771157916652209
30-31	0.00467821328191223
32-33	0.006661032156365097
34-35	0.0064751428868851406
36-37	0.005700604264051989
38-39	0.005979438168271924
40-41	0.006041401258098576
42-43	0.005576678084398686
44-45	0.0058245304437052935
46-47	0.007621460048678203
48-49	0.0068469214258450525
50-51	0.004740176371738882
52-53	0.0045233055573456
54-55	0.005142936455612121
56-57	0.006134345892838554
58-59	0.005514714994572034
60-61	0.004244471653125666
62-63	0.004616250192085579
64-65	0.003253062215899233
66-67	0.003315025305725885
68-69	0.005266862635265425
70-71	0.004120545473472362
72-73	0.0037487669345124494
74-75	0.0035318961201191677
76-77	0.003965637748905732
78-79	0.002757357497286017
80-81	0.0026334313176327125
82-83	0.004337416287865644
84-85	0.007559496958851551
86-87	0.005762567353878641
88-89	0.003965637748905732
90-91	0.0044303609226056225
92-93	0.006444161341971814
94-95	0.005669622719138664
96-97	0.0072806630546316175
98-99	0.005359807270005403
100-101	0.006661032156365097
102-103	0.004461342467518949
104-105	0.00405858238364571
106-107	0.0058245304437052935
108-109	0.0054527519047453815
110-111	0.006103364347925228
112-113	0.004337416287865644
114-115	0.002137726599019496
116-117	0.002757357497286017
118-119	0.13529640663649478
120-121	0.008798758755384592
122-123	0.0018279111498862358
124-125	0.0013322064312730193
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1613864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.11612244121321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72345663391691	50.949515727842645
2	10.625262844576358	13.412507813367094
3	3.4754250818093975	6.580660649962361
4	1.590070103954	4.014362374850931
5	0.9186014553236532	2.8989280964442177
6	0.581313896342059	2.201416743498247
7	0.4016140465033362	1.7743824933250965
8	0.29002342962715477	1.4644123436134469
9	0.220096044249158	1.2502447989890884
>10	1.1154253331214088	12.304545781801787
>50	0.04723573482412278	1.9680573354496826
>100	0.011181193547743318	1.072152072924229
>500	2.942022047476919E-4	0.10881376793115498
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.196308982665206E-5
6	0.0	0.0	0.0	0.0	6.196308982665206E-5
7	0.0	0.0	0.0	0.0	6.196308982665206E-5
8	0.0	0.0	0.0	0.0	6.196308982665206E-5
9	0.0	0.0	0.0	0.0	6.196308982665206E-5
10-11	0.0	0.0	0.0	0.0	6.196308982665206E-5
12-13	0.0	0.0	0.0	0.0	2.1687081439328222E-4
14-15	0.0	3.098154491332603E-5	0.0	0.0	3.098154491332603E-4
16-17	0.0	6.196308982665206E-5	0.0	0.0	3.7177853895991236E-4
18-19	0.0	6.196308982665206E-5	0.0	3.098154491332603E-5	4.957047186132165E-4
20-21	0.0	6.196308982665206E-5	0.0	1.5490772456663014E-4	6.196308982665205E-4
22-23	0.0	6.196308982665206E-5	0.0	2.7883390421993426E-4	7.125755330064987E-4
24-25	0.0	6.196308982665206E-5	0.0	4.027600838732384E-4	8.055201677464768E-4
26-27	0.0	6.196308982665206E-5	0.0	7.435570779198247E-4	8.055201677464768E-4
28-29	0.0	6.196308982665206E-5	0.0	0.0034079699404658634	8.365017126598028E-4
30-31	0.0	6.196308982665206E-5	0.0	0.013229119677990215	8.674832575731288E-4
32-33	0.0	6.196308982665206E-5	0.0	0.031694120446332526	9.294463473997809E-4
34-35	0.0	6.196308982665206E-5	0.0	0.055921688568553486	9.91409437226433E-4
36-37	0.0	6.196308982665206E-5	0.0	0.09158144676379174	9.91409437226433E-4
38-39	0.0	6.196308982665206E-5	0.0	0.14939300957205812	9.91409437226433E-4
40-41	0.0	6.196308982665206E-5	0.0	0.21934933798634831	9.91409437226433E-4
42-43	0.0	6.196308982665206E-5	0.0	0.2856188625559527	9.91409437226433E-4
44-45	0.0	6.196308982665206E-5	0.0	0.35309666737717676	0.001022390982139759
46-47	0.0	6.196308982665206E-5	0.0	0.42924930477413215	0.001053372527053085
48-49	0.0	6.196308982665206E-5	0.0	0.5144175717408654	0.001115335616879737
50-51	0.0	6.196308982665206E-5	0.0	0.6046048489835575	0.001115335616879737
52-53	0.0	6.196308982665206E-5	0.0	0.6922516395433568	0.001239261796533041
54-55	0.0	1.2392617965330412E-4	0.0	0.787860687145881	0.0013631879761863454
56-57	0.0	1.2392617965330412E-4	0.0	0.8857004059821645	0.0013941695210996715
58-59	0.0	1.8588926947995618E-4	0.0	0.993980905454239	0.0014251510660129973
60-61	0.0	1.8588926947995618E-4	0.0	1.1133837795501975	0.0014561326109263234
62-63	0.0	1.8588926947995618E-4	0.0	1.2475338690248994	0.0015490772456663016
64-65	0.0	2.4785235930660824E-4	0.0	1.3694772298037505	0.0016110403354929535
66-67	0.0	3.098154491332603E-4	0.0	1.5014586111345194	0.0016110403354929535
68-69	0.0	3.098154491332603E-4	0.0	1.6488688018321247	0.0016110403354929535
70-71	0.0	3.098154491332603E-4	0.0	1.8106544293695133	0.0017039849702329317
72-73	0.0	3.098154491332603E-4	0.0	1.9684434376130828	0.0017969296049729097
74-75	0.0	3.098154491332603E-4	0.0	2.1313134192224377	0.0017969296049729097
76-77	0.0	3.098154491332603E-4	0.0	2.3128033093247016	0.0017969296049729097
78-79	0.0	3.098154491332603E-4	0.0	2.5186137121839263	0.0018588926947995619
80-81	0.0	3.098154491332603E-4	0.0	2.7280489557980103	0.0018588926947995619
82-83	0.0	3.098154491332603E-4	0.0	2.950000743557078	0.001982818874452866
84-85	0.0	3.098154491332603E-4	0.0	3.1768785969573643	0.001982818874452866
86-87	0.0	3.098154491332603E-4	0.0	3.4230579528386533	0.002013800419366192
88-89	0.0	3.098154491332603E-4	0.0	3.695199843357309	0.002044781964279518
90-91	0.0	3.098154491332603E-4	0.0	3.9876656273391067	0.002044781964279518
92-93	0.0	3.098154491332603E-4	0.0	4.288682317716982	0.002292634323586126
94-95	0.0	3.098154491332603E-4	0.0	4.594408202921684	0.002478523593066082
96-97	0.0	3.098154491332603E-4	0.0	4.91630645457114	0.0026024497727193865
98-99	0.0	3.098154491332603E-4	0.0	5.276466914188557	0.0027263759523726908
100-101	0.0	3.098154491332603E-4	0.0	5.670954925569936	0.0027263759523726908
102-103	0.0	3.098154491332603E-4	0.0	6.0870680553008185	0.0028193205871126685
104-105	0.0	3.098154491332603E-4	0.0	6.516255396985124	0.0028503021320259946
106-107	0.0	3.098154491332603E-4	0.0	6.954830146778168	0.0028503021320259946
108-109	0.0	3.098154491332603E-4	0.0	7.417787372417998	0.0028812836769393207
110-111	0.0	3.098154491332603E-4	0.0	7.921051587990066	0.002974228311679299
112-113	0.0	3.098154491332603E-4	0.0	8.441789394893249	0.003036191401505951
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1440	0.0	59.095146	1
GTATCAA	2435	0.0	53.76529	1
TCAACGC	2920	0.0	44.220966	4
CAACGCA	3030	0.0	42.023823	5
TAGTACG	190	0.0	40.71375	2
GTACATG	8195	0.0	39.938522	1
AACGCAG	3270	0.0	39.12026	6
TATCAAC	3310	0.0	39.01064	2
TACATGG	8445	0.0	38.26058	2
ATCAACG	3410	0.0	37.69213	3
ACATGGG	8700	0.0	36.660374	3
CATGGGG	7940	0.0	35.897675	4
ATGGGGG	4675	0.0	33.60058	5
GTACGGT	130	1.7355796E-6	32.040997	4
ACGCAGA	4130	0.0	30.539112	7
CGCAGAG	4510	0.0	28.097887	8
GAGTACA	5745	0.0	26.310066	1
AGTACAT	5690	0.0	25.935272	2
TACGGTC	165	1.1100456E-5	25.242857	5
TGGGGGG	3025	0.0	25.17651	6
>>END_MODULE