FastQCFastQC Report
Mon 15 Aug 2016
SRR2921319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613864
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16300.062.300211
GTATCAA26450.050.814191
GTACATG74850.041.0203631
TACATGG78750.038.8152162
ACATGGG79350.038.071173
CATGGGG72050.036.3876384
TCAACGC36450.035.79994
TATCAAC40100.033.5814362
CAACGCA38950.033.349115
ATGGGGG42750.033.1723335
ATCAACG41200.031.5278593
AACGCAG41450.031.1920156
ATGGGGA25950.025.7167385
ACGCAGA51750.024.2876827
TGGGGGG28300.022.737656
CGCAGAG55350.022.6066842
AGAGTAC54950.022.4459445
TATAACG1350.002534686622.068432
GTGGTAT9450.021.4919221
CAGAGTA60250.021.1637154
Produced by FastQC (version 0.11.5)