##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921319_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1613864
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.47974364630477	33.0	30.0	34.0	23.0	34.0
2	30.21588869941953	33.0	31.0	34.0	26.0	34.0
3	30.453659044380444	33.0	31.0	34.0	27.0	34.0
4	32.68919066290592	37.0	35.0	37.0	19.0	37.0
5	32.35442453639216	37.0	35.0	37.0	19.0	37.0
6	32.51549634913475	37.0	35.0	37.0	17.0	37.0
7	32.71712982010876	37.0	35.0	37.0	17.0	37.0
8	32.96291137295336	37.0	35.0	37.0	17.0	37.0
9	34.7309488284019	39.0	37.0	39.0	24.0	39.0
10-11	34.9003782227003	39.0	37.0	39.0	26.5	39.0
12-13	34.92746445797168	39.0	37.0	39.0	27.0	39.0
14-15	36.33809943093098	41.0	38.0	41.0	27.0	41.0
16-17	36.36378901815766	41.0	38.0	41.0	27.0	41.0
18-19	36.380882156117245	41.0	38.0	41.0	27.0	41.0
20-21	36.379436557231585	41.0	38.0	41.0	27.0	41.0
22-23	36.33015204502982	41.0	38.0	41.0	27.0	41.0
24-25	36.27931071019616	41.0	38.0	41.0	27.0	41.0
26-27	36.19156477869262	41.0	38.0	41.0	27.0	41.0
28-29	36.1237678639588	40.5	38.0	41.0	26.5	41.0
30-31	36.03855963079913	40.0	38.0	41.0	25.5	41.0
32-33	35.92489670752926	40.0	37.5	41.0	25.0	41.0
34-35	35.848575530527974	40.0	37.0	41.0	25.0	41.0
36-37	35.7728773923949	40.0	37.0	41.0	24.5	41.0
38-39	35.68282116708719	40.0	37.0	41.0	24.0	41.0
40-41	35.5564768778534	40.0	37.0	41.0	23.5	41.0
42-43	35.4240973836705	40.0	36.0	41.0	22.5	41.0
44-45	35.28458098080136	40.0	36.0	41.0	21.5	41.0
46-47	35.09271877927756	40.0	35.5	41.0	18.5	41.0
48-49	34.89037335240144	40.0	35.0	41.0	15.5	41.0
50-51	34.70759772818528	40.0	35.0	41.0	13.5	41.0
52-53	34.515117754655904	39.5	35.0	41.0	11.0	41.0
54-55	34.31018660804132	39.0	35.0	41.0	8.5	41.0
56-57	34.05829456509346	39.0	35.0	41.0	8.0	41.0
58-59	33.975616284891416	39.0	35.0	41.0	7.0	41.0
60-61	33.72077479886781	38.0	35.0	41.0	4.0	41.0
62-63	33.4228438703633	37.0	34.5	40.0	2.0	41.0
64-65	33.106949222487145	37.0	34.0	40.0	2.0	41.0
66-67	32.776284123073566	36.0	34.0	39.0	2.0	41.0
68-69	32.432164977965925	35.5	34.0	39.0	2.0	41.0
70-71	32.093985304833616	35.0	34.0	38.0	2.0	40.5
72-73	31.749720546464882	35.0	33.5	37.0	2.0	39.5
74-75	31.423811733826394	35.0	33.0	37.0	2.0	39.0
76-77	31.135080465268448	35.0	33.0	36.0	2.0	39.0
78-79	30.884596223721452	35.0	33.0	36.0	2.0	37.0
80-81	30.666545322282424	35.0	33.0	35.0	2.0	37.0
82-83	30.47514319670059	35.0	33.0	35.0	2.0	36.5
84-85	30.31503769834385	35.0	33.0	35.0	2.0	36.0
86-87	30.183655809907155	35.0	33.0	35.0	2.0	36.0
88-89	30.07185487748658	35.0	33.0	35.0	2.0	36.0
90-91	29.97684532277813	35.0	32.0	35.0	2.0	35.0
92-93	29.916086485602257	35.0	32.0	35.0	2.0	35.0
94-95	29.854394794109044	35.0	32.0	35.0	2.0	35.0
96-97	29.79103474642225	35.0	32.0	35.0	2.0	35.0
98-99	29.734253629797802	35.0	32.0	35.0	2.0	35.0
100-101	29.686175848770404	35.0	32.0	35.0	2.0	35.0
102-103	29.007500941838963	34.0	30.5	35.0	2.0	35.0
104-105	29.560381481958828	35.0	32.0	35.0	2.0	35.0
106-107	29.62133953046849	35.0	32.0	35.0	2.0	35.0
108-109	29.696202096335256	35.0	32.5	35.0	2.0	35.0
110-111	29.80916545632098	35.0	33.0	35.0	2.0	35.0
112-113	29.771873280524257	35.0	33.0	35.0	2.0	35.0
114-115	29.7100015862551	35.0	33.0	35.0	2.0	35.0
116-117	29.64976014087928	35.0	33.0	35.0	2.0	35.0
118-119	29.573627641486517	35.0	33.0	35.0	2.0	35.0
120-121	29.48043236604819	35.0	33.0	35.0	2.0	35.0
122-123	29.367194199759087	35.0	32.5	35.0	2.0	35.0
124-125	28.839771814725403	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99767.0
3	6115.0
4	4564.0
5	4309.0
6	4386.0
7	4753.0
8	4955.0
9	5069.0
10	5258.0
11	5242.0
12	5512.0
13	6431.0
14	7544.0
15	6569.0
16	4595.0
17	4018.0
18	3704.0
19	3294.0
20	3320.0
21	3437.0
22	3888.0
23	4806.0
24	6272.0
25	8354.0
26	9932.0
27	11798.0
28	13942.0
29	16582.0
30	20270.0
31	25207.0
32	33167.0
33	44668.0
34	68036.0
35	116703.0
36	274766.0
37	580163.0
38	182269.0
39	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01577494903287	17.004624334941077	11.403585102680125	27.576015613345927
2	15.996205380379017	18.76267145186955	37.595547084512695	27.645576083238737
3	19.589259070157087	22.019079674619423	27.948575592491064	30.443085662732422
4	13.66321171275854	15.028150902471143	30.94535595321094	40.36328143155938
5	14.860930522670357	37.20030163010591	32.15457611970867	15.78419172751506
6	36.10944604407968	32.87844459665947	15.647387360023307	15.364721999237545
7	31.23363093578059	31.239208657615887	17.981149779184193	19.546010627419335
8	29.037325391602437	34.81726458927445	17.16469796511736	18.980712054005753
9	28.025557434515424	15.464404313607147	17.337959333910447	39.17207891796698
10-11	27.56744883071963	25.081661099697644	24.995561812565043	22.355328257017682
12-13	27.15902996710965	22.15823948268219	25.92705223707093	24.75567831313723
14-15	24.678690397703896	23.529337044509326	23.006647400276602	28.785325157510172
16-17	25.044923240124323	26.603325930809536	23.63182399508261	24.719926833983532
18-19	25.143940257667314	25.700771564394522	25.187748162174756	23.96754001576341
20-21	25.646956620880072	24.69765110319085	25.262196814601477	24.393195461327597
22-23	25.533223369503254	24.31016492095988	25.135606222085627	25.021005487451237
24-25	25.13517248045684	24.691950498926797	25.077794659277362	25.095082361339
26-27	24.920470374207493	24.730088780715104	25.337543931830687	25.011896913246716
28-29	24.94587524103642	25.083123485002456	25.15388533358449	24.817115940376635
30-31	25.302132025994755	24.87350235211889	25.050840715202767	24.773524906683587
32-33	24.976361081231133	25.012175747150938	25.139200081295577	24.87226309032236
34-35	25.072527796642095	25.074944357145334	25.320008377409746	24.532519468802825
36-37	25.190350611947476	24.9183946106983	25.28252070806462	24.608734069289607
38-39	25.072156018103136	25.149485954206796	25.20416838097882	24.574189646711247
40-41	25.017287702061637	25.190443556582213	25.169407087586066	24.62286165377008
42-43	24.878087620766063	25.089350775530033	25.038541041872175	24.99402056183173
44-45	24.767173689976353	25.16159973826791	25.254637317642626	24.81658925411311
46-47	24.74384458665662	25.10220811666906	24.978188992381018	25.1757583042933
48-49	24.83226591583925	25.31855224479882	24.799394496686215	25.049787342675717
50-51	24.752922179316226	25.42271219879742	24.9676862486554	24.856679373230953
52-53	24.908046774697247	25.29807344361111	24.927565147992645	24.866314633698998
54-55	24.50556552472823	25.07293055672597	25.193975452702333	25.22752846584347
56-57	24.668063727798625	25.260709700445638	24.891998334432145	25.179228237323592
58-59	24.715279602246536	25.305292143575915	25.000867483257572	24.978560770919977
60-61	25.034389514853792	25.083433300451585	25.14313473749957	24.739042447195057
62-63	24.773431962048846	25.23174195595168	25.076896194474873	24.917929887524597
64-65	24.7168286794922	25.002850302132025	25.07085479321678	25.209466225158998
66-67	24.61957761000927	25.218946577902475	25.013445990492382	25.148029821595873
68-69	24.47473888753947	25.13476972037297	25.049167711777446	25.34132368031011
70-71	24.478611580653638	25.011029429989147	25.187252457456143	25.32310653190108
72-73	24.570254990507255	25.314276791600783	24.805559824123964	25.309908393767998
74-75	24.361656248605833	25.354088076814403	24.986089286333918	25.298166388245853
76-77	24.41193929600016	24.98565554470513	24.982898187207844	25.61950697208687
78-79	24.35793846321623	24.982247574764667	25.14521050100876	25.514603461010342
80-81	24.531868856359644	25.182791114988625	24.904174081583083	25.381165947068652
82-83	24.523101079149175	25.21795516846525	25.01462328919909	25.244320463186487
84-85	24.495713393445794	25.23846495119787	24.775352817833472	25.49046883752286
86-87	24.16275473026228	25.37847055266119	25.007590478503765	25.451184238572765
88-89	24.16036915130395	25.122717899401685	25.011401208528106	25.705511740766262
90-91	24.35133024198808	24.933195898902042	25.070475422132212	25.644998436977666
92-93	24.367192043168593	25.11512006783742	25.061459849175126	25.456228039818857
94-95	24.32896988714029	25.007714414243598	25.02590060364517	25.637415094970944
96-97	24.09075023948823	24.93230507268917	25.082875940758107	25.894068747064487
98-99	24.00794738836339	25.021632870437926	25.029781019274555	25.94063872192413
100-101	24.019217232678837	25.091705372943444	25.09802560810576	25.79105178627195
102-103	23.821895773125863	25.000309815449135	25.106576514501842	26.07121789692316
104-105	23.87959080802063	25.129572606357353	25.00889945153354	25.98193713408848
106-107	23.911755721217627	25.00912408193766	25.078925632380976	26.00019456446373
108-109	23.788052050412105	24.944597063497202	25.19715947835575	26.07019140773494
110-111	23.733469735255518	25.262879385918712	25.090528410801944	25.91312246802383
112-113	23.716038573525594	25.32278342575191	25.085834955228265	25.87534304549423
114-115	23.645359572701473	25.32933463869233	24.972798136145638	26.05250765246056
116-117	23.386620805505324	25.210667739046194	25.176773771397894	26.225937684050592
118-119	23.546945551005386	25.14717828509182	25.083975737657376	26.221900426245416
120-121	23.411745606659302	25.098970606550864	25.219953614402215	26.26933017238762
122-123	23.333339529730623	25.246446211243857	25.09246573857028	26.327748520455234
124-125	23.19260581827397	25.218458686251964	24.94873327267145	26.64020222280261
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	44.0
2	48.0
3	57.5
4	49.5
5	59.5
6	127.0
7	308.5
8	467.5
9	525.5
10	516.0
11	472.0
12	469.5
13	499.5
14	540.0
15	585.0
16	636.5
17	678.5
18	730.0
19	792.5
20	821.0
21	878.5
22	965.5
23	1040.5
24	1157.0
25	1317.0
26	1560.5
27	1973.0
28	2490.5
29	3200.0
30	4241.5
31	5499.0
32	7120.0
33	9107.0
34	11224.5
35	13429.5
36	16338.5
37	19767.0
38	23050.0
39	26718.0
40	30871.0
41	34326.5
42	38156.5
43	43310.0
44	48138.0
45	52907.0
46	57831.5
47	62812.0
48	68144.5
49	72716.5
50	76064.5
51	78497.0
52	79108.5
53	78503.5
54	78321.0
55	77149.0
56	73271.5
57	67391.5
58	61597.0
59	55451.5
60	48040.5
61	40051.5
62	32357.5
63	25167.5
64	18406.5
65	13535.5
66	10220.5
67	7668.0
68	5751.5
69	4310.0
70	3322.0
71	2455.0
72	1824.0
73	1407.5
74	1044.0
75	785.0
76	598.5
77	427.5
78	288.5
79	222.5
80	189.5
81	155.5
82	150.0
83	143.5
84	127.5
85	112.0
86	113.0
87	109.5
88	98.0
89	88.0
90	83.5
91	82.0
92	72.0
93	72.0
94	66.5
95	59.5
96	58.0
97	46.0
98	33.5
99	17.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3088240396960339
2	0.0
3	0.0
4	0.014809178468569844
5	0.16228133225600175
6	0.04021404529749719
7	0.01871285312764892
8	0.006815939880931727
9	0.0053907888149187285
10-11	0.0018279111498862358
12-13	0.05756371044895977
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.1687081439328222E-4
92-93	3.4079699404658634E-4
94-95	1.2392617965330412E-4
96-97	3.7177853895991236E-4
98-99	3.098154491332603E-5
100-101	0.0
102-103	0.0
104-105	3.098154491332603E-5
106-107	1.8588926947995618E-4
108-109	3.4079699404658634E-4
110-111	3.7177853895991236E-4
112-113	6.815939880931727E-4
114-115	2.4785235930660824E-4
116-117	4.647231736998904E-4
118-119	3.0981544913326033E-4
120-121	6.196308982665206E-5
122-123	0.0014251510660129973
124-125	3.098154491332603E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1613864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.69684407288578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.59980462011198	53.439466799582156
2	9.520287551006755	12.318651184330394
3	3.06391520880114	5.9467693354905204
4	1.4498920085642286	3.7521374880241236
5	0.8571584376386631	2.772772289283349
6	0.5499411103235904	2.1347672558325006
7	0.3726249353054912	1.6875360135989965
8	0.26269458367982046	1.3596408415299994
9	0.20992565595667562	1.222337468729458
>10	1.0572043189894935	11.984863654604826
>50	0.04390145586730809	1.8907597698388081
>100	0.012266840961708061	1.2136924939793636
>500	9.58181982738378E-5	0.04196814417386588
>1k	2.874545948215134E-4	0.23463726100159876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.196308982665206E-5	0.0
9	0.0	0.0	0.0	6.196308982665206E-5	0.0
10-11	0.0	0.0	0.0	6.196308982665206E-5	0.0
12-13	0.0	0.0	0.0	6.196308982665206E-5	2.1687081439328222E-4
14-15	0.0	0.0	0.0	6.196308982665206E-5	2.4785235930660824E-4
16-17	0.0	0.0	0.0	6.196308982665206E-5	3.098154491332603E-4
18-19	0.0	0.0	0.0	9.294463473997809E-5	3.7177853895991236E-4
20-21	0.0	0.0	0.0	1.2392617965330412E-4	4.337416287865644E-4
22-23	0.0	0.0	0.0	1.5490772456663014E-4	5.886493533531946E-4
24-25	0.0	0.0	0.0	2.7883390421993426E-4	7.435570779198247E-4
26-27	0.0	0.0	0.0	8.055201677464768E-4	8.055201677464768E-4
28-29	0.0	0.0	0.0	0.0034699330302925156	8.055201677464768E-4
30-31	0.0	0.0	0.0	0.013105193498336911	8.055201677464768E-4
32-33	0.0	0.0	0.0	0.030857618733672723	8.674832575731288E-4
34-35	0.0	0.0	0.0	0.05369101733479401	9.91409437226433E-4
36-37	0.0	0.0	0.0	0.08742991974540605	9.91409437226433E-4
38-39	0.0	0.0	0.0	0.1418025310682932	9.91409437226433E-4
40-41	0.0	0.0	0.0	0.20788616636841767	9.91409437226433E-4
42-43	0.0	0.0	0.0	0.2701900531891163	9.91409437226433E-4
44-45	0.0	0.0	0.0	0.333640257171608	9.91409437226433E-4
46-47	0.0	0.0	0.0	0.40474290274769126	9.91409437226433E-4
48-49	0.0	0.0	0.0	0.48507804870794563	0.001115335616879737
50-51	0.0	0.0	0.0	0.5696886478662391	0.0011772987067063892
52-53	0.0	0.0	0.0	0.6525023174195596	0.0013012248863596932
54-55	0.0	3.098154491332603E-5	0.0	0.7439288564587847	0.0013631879761863454
56-57	0.0	6.196308982665206E-5	0.0	0.8359750263962762	0.0014251510660129975
58-59	0.0	6.196308982665206E-5	0.0	0.9375944937119856	0.0014871141558396494
60-61	0.0	6.196308982665206E-5	0.0	1.0510798927294989	0.0014871141558396494
62-63	0.0	6.196308982665206E-5	0.0	1.1777944114250023	0.0014871141558396494
64-65	0.0	6.196308982665206E-5	0.0	1.294068149484715	0.0014871141558396494
66-67	0.0	6.196308982665206E-5	0.0	1.4183661076769791	0.0015180957007529755
68-69	0.0	6.196308982665206E-5	0.0	1.557659133607293	0.0015490772456663016
70-71	0.0	9.294463473997809E-5	0.0	1.7106150208443833	0.0016110403354929535
72-73	0.0	1.2392617965330412E-4	0.0	1.860255882775748	0.0017969296049729097
74-75	0.0	1.2392617965330412E-4	0.0	2.0131498069230123	0.0018279111498862358
76-77	0.0	1.2392617965330412E-4	0.0	2.1839510640301785	0.0018588926947995619
78-79	0.0	1.2392617965330412E-4	0.0	2.3789179261697395	0.0018588926947995619
80-81	0.0	1.2392617965330412E-4	0.0	2.576951961255719	0.0018898742397128877
82-83	0.0	1.2392617965330412E-4	0.0	2.7870687988578964	0.002044781964279518
84-85	0.0	1.2392617965330412E-4	0.0	3.0013681450233722	0.002044781964279518
86-87	0.0	1.2392617965330412E-4	0.0	3.2342873996817576	0.002075763509192844
88-89	0.0	1.2392617965330412E-4	0.0	3.4937888198757765	0.00210674505410617
90-91	0.0	1.2392617965330412E-4	0.0	3.7716313146584843	0.00210674505410617
92-93	0.0	1.2392617965330412E-4	0.0	4.058520420555883	0.00210674505410617
94-95	0.0	1.2392617965330412E-4	0.0	4.346834677519295	0.002168708143932822
96-97	0.0	1.2392617965330412E-4	0.0	4.65221976572995	0.0023545974134127783
98-99	0.0	1.2392617965330412E-4	0.0	4.9946897632018565	0.0024165605032394305
100-101	0.0	1.2392617965330412E-4	0.0	5.369938235192061	0.0024165605032394305
102-103	0.0	1.2392617965330412E-4	0.0	5.7643333019387	0.002478523593066082
104-105	0.0	1.2392617965330412E-4	0.0	6.1728559531658185	0.0026024497727193865
106-107	0.0	1.2392617965330412E-4	0.0	6.591633495759246	0.0026644128625460386
108-109	0.0	1.2392617965330412E-4	0.0	7.034576643385068	0.0026644128625460386
110-111	0.0	1.2392617965330412E-4	0.0	7.512745807577343	0.0026953944074593647
112-113	0.0	1.2392617965330412E-4	0.0	8.011703588406458	0.002788339042199343
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1630	0.0	62.30021	1
GTATCAA	2645	0.0	50.81419	1
GTACATG	7485	0.0	41.020363	1
TACATGG	7875	0.0	38.815216	2
ACATGGG	7935	0.0	38.07117	3
CATGGGG	7205	0.0	36.387638	4
TCAACGC	3645	0.0	35.7999	4
TATCAAC	4010	0.0	33.581436	2
CAACGCA	3895	0.0	33.34911	5
ATGGGGG	4275	0.0	33.172333	5
ATCAACG	4120	0.0	31.527859	3
AACGCAG	4145	0.0	31.192015	6
ATGGGGA	2595	0.0	25.716738	5
ACGCAGA	5175	0.0	24.287682	7
TGGGGGG	2830	0.0	22.73765	6
CGCAGAG	5535	0.0	22.606684	2
AGAGTAC	5495	0.0	22.445944	5
TATAACG	135	0.0025346866	22.06843	2
GTGGTAT	945	0.0	21.491922	1
CAGAGTA	6025	0.0	21.163715	4
>>END_MODULE