FastQCFastQC Report
Mon 15 Aug 2016
SRR2921318_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921318_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1342738
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.059.7500531
GTATCAA22900.053.5286751
TCAACGC26550.044.8233224
CAACGCA26600.044.2916765
GTACATG75900.043.0412941
TACATGG77350.041.5405272
ATCAACG29050.041.1707273
AACGCAG28700.041.0492946
CATGGGG67150.040.4070744
ACATGGG78600.040.3499763
TATCAAC30350.039.99542
ATGGGGG44600.038.0231935
ACGCAGA37400.031.4957037
TGGGGGG34600.030.610216
CGCAGAG40600.029.1587288
CGCTAGT850.007209042628.0013942
AGTACAT48650.027.0301232
GAGTACA49850.026.8579031
ATGGGGA21000.026.3513135
GCAGAGT45200.025.7964049
Produced by FastQC (version 0.11.5)