##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921318_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1342738
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.220682664823666	31.0	31.0	34.0	26.0	34.0
2	30.382081239973843	31.0	31.0	34.0	26.0	34.0
3	30.463930416805066	31.0	31.0	34.0	26.0	34.0
4	33.90085556527036	37.0	35.0	37.0	30.0	37.0
5	33.96843464622287	37.0	35.0	37.0	30.0	37.0
6	34.2200861225347	37.0	35.0	37.0	31.0	37.0
7	34.24128608857424	37.0	35.0	37.0	31.0	37.0
8	34.2303733118449	37.0	35.0	37.0	31.0	37.0
9	35.84716675926354	39.0	37.0	39.0	31.0	39.0
10-11	35.84462754461406	39.0	37.0	39.0	31.0	39.0
12-13	35.76217586751846	39.0	37.0	39.0	30.0	39.0
14-15	37.088198889135484	41.0	38.0	41.0	31.0	41.0
16-17	37.077743014646195	41.0	38.0	41.0	31.0	41.0
18-19	37.07244860873826	41.0	38.0	41.0	31.0	41.0
20-21	37.104475705610476	41.0	38.0	41.0	31.0	41.0
22-23	37.066685757012905	41.0	38.0	41.0	30.0	41.0
24-25	37.06616592365748	41.0	38.0	41.0	30.0	41.0
26-27	37.039343863061895	41.0	38.0	41.0	30.0	41.0
28-29	36.979943965241176	40.0	38.0	41.0	30.0	41.0
30-31	36.90966927278441	40.0	38.0	41.0	30.0	41.0
32-33	36.83044681836665	40.0	38.0	41.0	30.0	41.0
34-35	36.72586610343939	40.0	38.0	41.0	30.0	41.0
36-37	36.6180025440555	40.0	37.0	41.0	30.0	41.0
38-39	36.521577552731806	40.0	37.0	41.0	29.5	41.0
40-41	36.417714773842704	40.0	37.0	41.0	29.0	41.0
42-43	36.23014095080351	40.0	36.5	41.0	28.0	41.0
44-45	36.003639578234925	40.0	36.0	41.0	28.0	41.0
46-47	35.79612441146374	40.0	35.5	41.0	27.0	41.0
48-49	35.599473612871606	40.0	35.0	41.0	27.0	41.0
50-51	35.42559940956464	39.5	35.0	41.0	26.5	41.0
52-53	35.247973543610144	39.0	35.0	41.0	26.0	41.0
54-55	35.04926426451028	39.0	35.0	41.0	26.0	41.0
56-57	34.81204933501547	39.0	35.0	40.5	26.0	41.0
58-59	34.552787662224496	38.0	34.5	40.0	25.0	41.0
60-61	34.27082014510649	38.0	34.0	40.0	24.5	41.0
62-63	33.9407818204296	37.0	34.0	40.0	23.5	41.0
64-65	33.602379987756365	37.0	34.0	39.5	22.5	41.0
66-67	33.26698879453773	36.0	33.5	39.0	22.0	41.0
68-69	32.91029932868512	36.0	33.0	39.0	22.0	40.5
70-71	32.85982782940529	35.0	33.0	39.0	22.0	40.0
72-73	32.56221727544763	35.0	33.0	37.5	21.5	40.0
74-75	32.22881455652555	35.0	33.0	37.0	20.5	39.0
76-77	31.910012601118012	35.0	33.0	36.5	20.0	39.0
78-79	31.609877727449437	35.0	33.0	36.0	19.5	38.5
80-81	31.3286300082369	35.0	33.0	36.0	18.0	37.0
82-83	31.098526667153237	35.0	33.0	35.0	18.0	37.0
84-85	30.867356103722393	35.0	32.0	35.0	15.5	36.5
86-87	30.685207389676915	35.0	32.0	35.0	12.5	36.0
88-89	30.539811936505856	35.0	32.0	35.0	10.5	36.0
90-91	30.40980705096601	35.0	32.0	35.0	7.0	35.5
92-93	30.2890426129297	35.0	32.0	35.0	7.0	35.0
94-95	30.156831786990466	35.0	32.0	35.0	6.0	35.0
96-97	30.061045043783672	35.0	32.0	35.0	2.0	35.0
98-99	29.971813563033145	35.0	31.5	35.0	2.0	35.0
100-101	29.879333496184664	35.0	31.0	35.0	2.0	35.0
102-103	29.04585369595558	33.5	29.5	34.5	2.0	35.0
104-105	29.634295372589442	34.0	31.0	35.0	2.0	35.0
106-107	29.69678485303909	35.0	31.0	35.0	2.0	35.0
108-109	29.632947753024045	35.0	31.0	35.0	2.0	35.0
110-111	29.55258248444596	35.0	31.0	35.0	2.0	35.0
112-113	29.45919196447855	34.0	31.0	35.0	2.0	35.0
114-115	29.323651002652788	34.0	31.0	35.0	2.0	35.0
116-117	29.185148182296174	34.0	31.0	35.0	2.0	35.0
118-119	29.186229554834973	34.5	30.5	35.0	2.0	35.0
120-121	29.36223857520976	35.0	31.5	35.0	2.0	35.0
122-123	29.22616400221041	35.0	31.0	35.0	2.0	35.0
124-125	28.647089752431228	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43571.0
3	6310.0
4	4080.0
5	3503.0
6	3730.0
7	4332.0
8	4343.0
9	4506.0
10	5149.0
11	5753.0
12	5626.0
13	5599.0
14	5730.0
15	5274.0
16	4509.0
17	4109.0
18	3793.0
19	3634.0
20	3477.0
21	3735.0
22	4219.0
23	5449.0
24	7917.0
25	10242.0
26	10561.0
27	11617.0
28	13910.0
29	16481.0
30	19985.0
31	25589.0
32	32892.0
33	45991.0
34	73424.0
35	120562.0
36	250921.0
37	439131.0
38	122982.0
39	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.65147600060484	17.66411200487444	11.548491522987113	25.135920471533606
2	16.701003284695997	19.44108030001713	38.778182468214425	25.07973394707245
3	20.828268473332024	23.468773648427213	28.112952336688057	27.59000554155271
4	13.571981885353354	14.803956620188297	34.63547253009177	36.98858896436658
5	14.18379180361935	38.67983199974378	32.58464625429292	14.551729942343943
6	34.475291144860634	33.67312959959713	17.127281314507965	14.724297941034273
7	30.29689106049546	32.4330766136841	18.89362272639247	18.376409599427966
8	28.21897442696408	35.14292566733155	18.5335613510047	18.104538554699662
9	28.381206606079658	16.0682445506695	17.880864742798156	37.66968410045269
10-11	26.970112363985876	25.727714426481153	26.022729007768618	21.279444201764353
12-13	26.229325663027392	22.938003242799592	26.550514759057904	24.28215633511511
14-15	23.845505600194837	24.40405414111292	23.760488844483387	27.989951414208857
16-17	24.407235613230732	27.526342601281605	24.37602964790138	23.690392137586283
18-19	24.287007552497137	27.01940203408362	25.7434727931727	22.950117620246544
20-21	25.022250258996735	25.97039058421174	25.668196690055193	23.33916246673633
22-23	24.850718506213127	25.36912141859406	25.664016373205413	24.116143701987404
24-25	24.35348543454679	25.390887854556617	25.834999465621195	24.4206272452754
26-27	23.948354557462295	25.829908314890925	25.804921227368716	24.41681590027806
28-29	24.06915438336994	26.607776851767028	25.457351246800236	23.865717518062795
30-31	25.085584205063967	25.87482455843093	25.51222979950227	23.52736143700284
32-33	24.615537011824358	25.791269815261142	25.56220822670357	24.030984946210932
34-35	24.41625985499642	26.153524747394435	25.764557055360026	23.665658342249117
36-37	24.28851415337625	26.09961304584053	25.87040480298332	23.741467997799898
38-39	24.45461079271449	26.389531268037818	25.540734285916443	23.61512365333125
40-41	24.580375426811806	25.89213861901593	25.707653882204596	23.819832071967667
42-43	24.095742600988707	26.016627410090027	25.47956020221016	24.408069786711106
44-45	23.727565090841924	26.14194786178502	26.023154003017883	24.10733304435517
46-47	23.721742938667344	25.823961794360166	25.88190780923099	24.572387457741492
48-49	23.776999301009457	26.187716013958955	25.80362499399509	24.231659691036498
50-51	23.864520364658333	26.149797997514675	25.976599811645578	24.009081826181415
52-53	24.080990167809716	26.042736121708685	25.74839975079719	24.127873959684408
54-55	23.601482715063693	25.747613713550294	26.336514055554893	24.31438951583112
56-57	23.77672820249661	26.05135553727509	25.673746769005845	24.49816949122246
58-59	23.82827119378757	26.21531753860889	25.9074593150108	24.048951952592738
60-61	24.46370598657766	26.14946744848204	25.933221964612752	23.45360460032755
62-63	24.239860398709745	26.201309171724336	25.80929498518263	23.74953544438329
64-65	23.913534052976757	25.844267752636103	25.802262899735013	24.439935294652127
66-67	23.939298247704617	26.009126414318658	25.70943087638602	24.34214446159071
68-69	23.66485498138973	25.954058044683272	25.995989342151564	24.38509763177543
70-71	23.68565205458741	25.81771450680555	26.17401404121054	24.322619397396497
72-73	23.809788026512418	26.4796492716309	25.530585395081445	24.179977306775235
74-75	23.700397482980176	26.29487323666734	25.699281819678127	24.30544746067436
76-77	23.666802463407496	25.76899559318474	25.88614821109517	24.6780537323126
78-79	23.521015622585328	25.803276747249114	26.263765604245464	24.411942025920094
80-81	23.89069970347161	26.06839405096207	25.712470614345897	24.328435631220422
82-83	24.105382988619432	26.059641009962515	25.88610793236083	23.948868069057227
84-85	23.984670391444258	26.331555852485106	25.461696274589325	24.222077481481318
86-87	23.466023665664302	26.535410052786908	25.787902767821404	24.210663513727386
88-89	23.417748387380268	26.022145080290255	26.015963464415865	24.544143067913616
90-91	23.95622196610144	25.72782470911775	26.068150884516626	24.247802440264174
92-93	24.119976300573086	25.884957976715423	25.80902596621528	24.186039756496207
94-95	24.015868399385255	25.830016865667137	25.7468243278218	24.40729040712581
96-97	23.610210102403414	25.75622043584574	25.92022677861602	24.713342683134833
98-99	23.348417738724496	25.80230243239761	25.9023644730972	24.946915355780696
100-101	23.43126057379958	26.021741389114705	26.015373368939628	24.531624668146083
102-103	23.332536421478487	26.03376969843217	25.97340548737118	24.66028839271816
104-105	23.571384150989488	26.063062234418304	25.72921736559346	24.636336248998745
106-107	23.557041331448513	25.926073504943183	26.00524482034889	24.511640343259415
108-109	23.496086883985868	25.844068210661792	25.99012113231605	24.669723773036292
110-111	23.300558706651636	26.440495302402816	25.932546310312894	24.326399680632658
112-113	23.222647158492126	26.546285986661054	25.888760208090506	24.342306646756313
114-115	23.34324114180852	26.732426611216702	25.66522083663511	24.25911141033967
116-117	23.171703081355577	26.51882823063452	25.76318914548957	24.546279542520338
118-119	23.01816835178064	26.223334567753444	26.05073951460811	24.707757565857804
120-121	23.084024650567628	26.305483514895656	26.190818453068136	24.419673381468577
122-123	23.19678055643168	26.477294350668963	25.825630354583414	24.500294738315944
124-125	23.037900679218303	26.35251578884652	25.760247855100094	24.84933567683508
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	566.0
1	494.5
2	374.5
3	298.5
4	246.5
5	200.5
6	191.5
7	227.0
8	274.0
9	321.5
10	336.5
11	313.0
12	313.0
13	371.0
14	430.0
15	462.0
16	503.5
17	543.5
18	603.0
19	664.5
20	713.5
21	797.5
22	936.5
23	1168.5
24	1389.0
25	1731.5
26	2371.5
27	3122.5
28	3865.0
29	4848.5
30	6391.5
31	8203.0
32	10500.5
33	13117.5
34	15992.5
35	18904.0
36	21999.0
37	24874.5
38	27003.0
39	29654.5
40	32253.5
41	34262.5
42	36292.5
43	39628.5
44	43220.0
45	46555.5
46	49934.0
47	52880.0
48	56620.5
49	60175.0
50	62107.0
51	63279.5
52	62557.5
53	60345.0
54	58607.0
55	55783.5
56	51446.0
57	46418.5
58	41938.5
59	37128.5
60	31527.5
61	26766.0
62	21759.5
63	16279.0
64	11964.5
65	9052.0
66	6959.5
67	5239.5
68	3900.5
69	3094.0
70	2481.5
71	1898.0
72	1395.5
73	1100.5
74	896.0
75	649.5
76	459.0
77	282.0
78	160.5
79	101.0
80	65.5
81	45.5
82	36.0
83	32.0
84	26.0
85	17.0
86	11.5
87	13.0
88	11.0
89	10.5
90	8.5
91	4.5
92	5.0
93	3.5
94	1.0
95	2.0
96	2.5
97	2.5
98	2.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016756805869797383
2	0.011022254527688947
3	0.011469102684216876
4	0.013256495310328596
5	0.00901143782331326
6	0.02718326285544909
7	0.011767001455235498
8	0.011320153298707567
9	0.0071495705044468845
10-11	0.0068889090798055904
12-13	0.005287703185580508
14-15	0.004580193604411285
16-17	0.003425835866714132
18-19	0.005175991146448525
20-21	0.004245057487015338
22-23	0.00420782014063801
24-25	0.004282294833392665
26-27	0.003314123827582149
28-29	0.004542956258033958
30-31	0.004058870755128699
32-33	0.006404823576900334
34-35	0.006069687459504386
36-37	0.00539941522471249
38-39	0.005436652571089818
40-41	0.005920738073995076
42-43	0.005622839302976456
44-45	0.005660076649353783
46-47	0.008117741510257398
48-49	0.006516535616032316
50-51	0.00420782014063801
52-53	0.00420782014063801
54-55	0.0049153297218072324
56-57	0.005846263381240421
58-59	0.0055483646102218
60-61	0.003798209330487407
62-63	0.004245057487015338
64-65	0.0026810889391675817
66-67	0.0031279370956955117
68-69	0.004878092375429905
70-71	0.0035375479058461145
72-73	0.0034630732130914595
74-75	0.0029045130174315467
76-77	0.0033513611739594768
78-79	0.002569376900035599
80-81	0.002494902207280944
82-83	0.004058870755128699
84-85	0.008006029471125418
86-87	0.005622839302976456
88-89	0.0035003105594687872
90-91	0.004133345447883355
92-93	0.006665485001541627
94-95	0.005175991146448525
96-97	0.00796879212474809
98-99	0.005287703185580508
100-101	0.006814434387050937
102-103	0.004096108101506027
104-105	0.00361202259860077
106-107	0.005957975420372403
108-109	0.005511127263844473
110-111	0.006218636845013696
112-113	0.003947158715996718
114-115	0.0019363420116210311
116-117	0.002494902207280944
118-119	0.1509974395600631
120-121	0.009830659443614465
122-123	0.0016012058942250836
124-125	0.0013405444695837908
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1342738.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.76644490418039
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.4043643457977	53.370377232661134
2	9.919741085370015	12.849327289387032
3	3.003647749124362	5.8360675936568525
4	1.418354043663675	3.6744699609425946
5	0.821336435098023	2.6597520485786017
6	0.5218936999565944	2.0280719738446575
7	0.3604494352439921	1.6341519941931064
8	0.26417660546962174	1.3687823650497322
9	0.1919363761938924	1.1187933060482795
>10	1.0317447079029234	11.683561118918288
>50	0.0477355446217501	2.1043331127636535
>100	0.014159513391408607	1.4099508612563734
>500	2.3022908302916902E-4	0.09610216338159201
>1k	2.3022908302916902E-4	0.1662589793180374
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.447469275465504E-5
7	0.0	0.0	0.0	0.0	7.447469275465504E-5
8	0.0	0.0	0.0	0.0	7.447469275465504E-5
9	0.0	0.0	0.0	0.0	7.447469275465504E-5
10-11	0.0	0.0	0.0	0.0	7.447469275465504E-5
12-13	0.0	0.0	0.0	0.0	1.4894938550931008E-4
14-15	0.0	0.0	0.0	0.0	1.8618673188663758E-4
16-17	0.0	0.0	0.0	3.723734637732752E-5	2.234240782639651E-4
18-19	0.0	0.0	0.0	1.1171203913198256E-4	2.234240782639651E-4
20-21	0.0	0.0	0.0	1.4894938550931008E-4	2.9789877101862015E-4
22-23	0.0	0.0	0.0	3.3513611739594766E-4	4.468481565279302E-4
24-25	0.0	0.0	0.0	7.075095811692228E-4	5.957975420372403E-4
26-27	0.0	0.0	0.0	0.0013777818159611183	5.957975420372403E-4
28-29	0.0	0.0	0.0	0.0061069248058817135	5.957975420372403E-4
30-31	0.0	0.0	0.0	0.020219879082888845	5.957975420372403E-4
32-33	0.0	0.0	0.0	0.04416349280351044	8.192216203012054E-4
34-35	0.0	0.0	0.0	0.07417679398363641	8.936963130558605E-4
36-37	0.0	0.0	0.0	0.1166273688537898	8.936963130558605E-4
38-39	0.0	0.0	0.0	0.1909158748765582	9.309336594331881E-4
40-41	0.0	0.0	0.0	0.2875467887257231	0.001079883044942498
42-43	0.0	0.0	0.0	0.37218727704138854	0.0011171203913198256
44-45	0.0	0.0	0.0	0.4630464022020677	0.0011171203913198256
46-47	0.0	0.0	0.0	0.5521553720830125	0.0011171203913198256
48-49	0.0	0.0	0.0	0.6534781915757206	0.0011915950840744806
50-51	0.0	0.0	0.0	0.7739782444527525	0.0011915950840744806
52-53	0.0	0.0	0.0	0.8929143287819366	0.0013777818159611183
54-55	0.0	0.0	0.0	1.0182179993416436	0.0015639685478477559
56-57	0.0	0.0	0.0	1.1464261829187823	0.0016012058942250834
58-59	0.0	0.0	0.0	1.28941759300772	0.0016384432406024109
60-61	0.0	0.0	0.0	1.4454048369823451	0.0016756805869797384
62-63	0.0	0.0	0.0	1.6138665919933748	0.001861867318866376
64-65	0.0	0.0	0.0	1.7748064030361843	0.0018991046652437034
66-67	0.0	0.0	0.0	1.9527264440270553	0.001973579357998359
68-69	0.0	0.0	0.0	2.1450201007195746	0.002010816704375686
70-71	0.0	0.0	0.0	2.3597306399312448	0.002085291397130341
72-73	0.0	0.0	0.0	2.558875968357192	0.002308715475394306
74-75	0.0	0.0	0.0	2.7565690402744245	0.0023459528217716335
76-77	0.0	0.0	0.0	2.9840519892935182	0.002383190168148961
78-79	0.0	0.0	0.0	3.2484371485725436	0.002383190168148961
80-81	0.0	0.0	0.0	3.5141628523211526	0.0024949022072809435
82-83	0.0	0.0	0.0	3.792884389955449	0.0027555636319222367
84-85	0.0	0.0	0.0	4.074138067143404	0.0027555636319222367
86-87	0.0	0.0	0.0	4.371515515312741	0.0028300383246768917
88-89	0.0	0.0	0.0	4.712944744246458	0.0029045130174315467
90-91	0.0	0.0	0.0	5.086584277796562	0.0029045130174315467
92-93	0.0	0.0	0.0	5.4389612865652115	0.0029789877101862017
94-95	0.0	0.0	0.0	5.8016158029340055	0.0029789877101862017
96-97	0.0	0.0	0.0	6.199794747746768	0.0029789877101862017
98-99	0.0	0.0	0.0	6.646829091006586	0.0029789877101862017
100-101	0.0	0.0	0.0	7.128233504972675	0.0029789877101862017
102-103	0.0	0.0	0.0	7.638124488917421	0.0033513611739594768
104-105	0.0	0.0	0.0	8.169501421721884	0.003425835866714132
106-107	0.0	0.0	0.0	8.724039983973046	0.0035375479058461145
108-109	0.0	0.0	0.0	9.299878308352039	0.0037237346377327523
110-111	0.0	0.0	0.0	9.916379814975073	0.003947158715996718
112-113	0.0	0.0	0.0	10.534035679335805	0.004021633408751372
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1215	0.0	59.750053	1
GTATCAA	2290	0.0	53.528675	1
TCAACGC	2655	0.0	44.823322	4
CAACGCA	2660	0.0	44.291676	5
GTACATG	7590	0.0	43.041294	1
TACATGG	7735	0.0	41.540527	2
ATCAACG	2905	0.0	41.170727	3
AACGCAG	2870	0.0	41.049294	6
CATGGGG	6715	0.0	40.407074	4
ACATGGG	7860	0.0	40.349976	3
TATCAAC	3035	0.0	39.9954	2
ATGGGGG	4460	0.0	38.023193	5
ACGCAGA	3740	0.0	31.495703	7
TGGGGGG	3460	0.0	30.61021	6
CGCAGAG	4060	0.0	29.158728	8
CGCTAGT	85	0.0072090426	28.001394	2
AGTACAT	4865	0.0	27.030123	2
GAGTACA	4985	0.0	26.857903	1
ATGGGGA	2100	0.0	26.351313	5
GCAGAGT	4520	0.0	25.796404	9
>>END_MODULE