FastQCFastQC Report
Mon 15 Aug 2016
SRR2921318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1342738
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.1818671997068676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.153715765845608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16400.12213849611763426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.073.616741
GTATCAA31750.046.1558842
GTACATG70450.041.0408481
TACATGG70450.040.5878372
ACATGGG71650.039.7417873
CATGGGG61950.036.4446334
ATGGGGG41950.035.6432655
TCAACGC42150.035.3328065
CAACGCA43150.034.513976
ATCAACG44800.033.641724
TATCAAC45050.033.1905633
AACGCAG44850.033.0667577
TGGGGGG32900.030.2382226
GTGGTAT9200.026.6224481
ACGCAGA57100.025.867488
CGCAGAG60150.024.5558289
GAGTACA48200.023.6721821
AGTACAT46500.023.4441132
TGGTATC8750.023.1476332
GTATAGG4450.022.8213231
Produced by FastQC (version 0.11.5)