##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921318_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1342738
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.21945234289936	31.0	30.0	34.0	19.0	34.0
2	29.99445014589592	33.0	31.0	34.0	26.0	34.0
3	30.23250254331076	33.0	31.0	34.0	26.0	34.0
4	32.393837814972095	37.0	35.0	37.0	19.0	37.0
5	32.080123598200096	37.0	33.0	37.0	19.0	37.0
6	32.21987759339499	37.0	35.0	37.0	17.0	37.0
7	32.44247127883474	37.0	35.0	37.0	17.0	37.0
8	32.687613666999816	37.0	35.0	37.0	17.0	37.0
9	34.42103597276609	39.0	37.0	39.0	17.0	39.0
10-11	34.617534470611545	39.0	37.0	39.0	23.5	39.0
12-13	34.63564150266098	39.0	37.0	39.0	25.0	39.0
14-15	36.029278235962636	40.5	38.0	41.0	25.5	41.0
16-17	36.040509764376964	41.0	38.0	41.0	26.0	41.0
18-19	36.07497553506343	41.0	38.0	41.0	26.0	41.0
20-21	36.06827541932976	41.0	38.0	41.0	26.0	41.0
22-23	36.01916680692734	41.0	38.0	41.0	25.0	41.0
24-25	35.964618562966116	41.0	38.0	41.0	25.0	41.0
26-27	35.86106857778658	40.5	38.0	41.0	24.5	41.0
28-29	35.78893015614364	40.0	37.0	41.0	24.0	41.0
30-31	35.688455231027945	40.0	37.0	41.0	24.0	41.0
32-33	35.55001981026827	40.0	36.5	41.0	23.0	41.0
34-35	35.46391552186651	40.0	36.0	41.0	22.0	41.0
36-37	35.38123483509069	40.0	36.0	41.0	20.5	41.0
38-39	35.28589158867925	40.0	36.0	41.0	18.5	41.0
40-41	35.148652231485215	40.0	35.5	41.0	17.0	41.0
42-43	35.00182909845405	40.0	35.0	41.0	15.0	41.0
44-45	34.843925248261385	40.0	35.0	41.0	11.0	41.0
46-47	34.61307269176861	40.0	35.0	41.0	7.0	41.0
48-49	34.38238360722643	40.0	35.0	41.0	2.0	41.0
50-51	34.19663962738822	40.0	35.0	41.0	2.0	41.0
52-53	34.021817361242476	39.0	35.0	41.0	2.0	41.0
54-55	33.82828630752984	39.0	35.0	41.0	2.0	41.0
56-57	33.59924199657715	39.0	34.5	41.0	2.0	41.0
58-59	33.54515624045793	39.0	35.0	41.0	2.0	41.0
60-61	33.31105770448144	38.0	34.0	41.0	2.0	41.0
62-63	33.0281510614878	37.0	34.0	40.5	2.0	41.0
64-65	32.732908430386274	37.0	34.0	40.0	2.0	41.0
66-67	32.42240146625775	36.0	34.0	39.5	2.0	41.0
68-69	32.092178071969364	36.0	34.0	39.0	2.0	41.0
70-71	31.761149978625763	35.0	33.0	39.0	2.0	41.0
72-73	31.41614745393368	35.0	33.0	37.0	2.0	40.0
74-75	31.074967342847227	35.0	33.0	37.0	2.0	39.0
76-77	30.773140776532728	35.0	33.0	36.5	2.0	39.0
78-79	30.507189042091607	35.0	33.0	36.0	2.0	38.0
80-81	30.270516288360053	35.0	32.5	36.0	2.0	37.0
82-83	30.060538988246407	35.0	32.0	35.0	2.0	37.0
84-85	29.878783128205207	35.0	32.0	35.0	2.0	36.0
86-87	29.733967460517242	35.0	32.0	35.0	2.0	36.0
88-89	29.602974668178007	35.0	32.0	35.0	2.0	36.0
90-91	29.496446067661747	35.0	32.0	35.0	2.0	35.5
92-93	29.425937897043205	35.0	32.0	35.0	2.0	35.0
94-95	29.349636712448742	35.0	32.0	35.0	2.0	35.0
96-97	29.28411015402856	35.0	32.0	35.0	2.0	35.0
98-99	29.22858368497801	35.0	31.5	35.0	2.0	35.0
100-101	29.174479310185607	35.0	31.0	35.0	2.0	35.0
102-103	28.497846936632463	34.0	29.5	35.0	2.0	35.0
104-105	29.037626849020434	35.0	31.0	35.0	2.0	35.0
106-107	29.09078129910675	35.0	31.0	35.0	2.0	35.0
108-109	29.163964228315578	35.0	31.5	35.0	2.0	35.0
110-111	29.266576949486794	35.0	32.0	35.0	2.0	35.0
112-113	29.22064468273036	35.0	32.0	35.0	2.0	35.0
114-115	29.155694930805563	35.0	32.0	35.0	2.0	35.0
116-117	29.08665800774239	35.0	32.0	35.0	2.0	35.0
118-119	29.003903218647274	35.0	32.0	35.0	2.0	35.0
120-121	28.903608149914575	35.0	32.0	35.0	2.0	35.0
122-123	28.78442518197891	35.0	32.0	35.0	2.0	35.0
124-125	28.252232378915323	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89102.0
3	5922.0
4	4207.0
5	4057.0
6	4058.0
7	4319.0
8	4560.0
9	4481.0
10	4810.0
11	4831.0
12	5449.0
13	6925.0
14	8522.0
15	7492.0
16	5077.0
17	4100.0
18	3766.0
19	3231.0
20	3063.0
21	3146.0
22	3541.0
23	4403.0
24	5839.0
25	7244.0
26	8877.0
27	9941.0
28	11993.0
29	14168.0
30	17041.0
31	21470.0
32	27288.0
33	36904.0
34	57021.0
35	96362.0
36	207526.0
37	458501.0
38	173160.0
39	341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1431363933897	17.643371023615455	11.350050363303236	26.863442219691603
2	16.037305863094662	19.589450808720688	37.681587919609036	26.69165540857561
3	19.766328204012996	22.98810341257937	28.459386715800107	28.78618166760753
4	13.408410880111552	15.595263544833124	31.51275620603893	39.483569369016394
5	13.76708557821282	38.209999865717535	32.47084205547656	15.55207250059308
6	34.242300969615144	33.71629182927502	16.28646487585141	15.754942325258423
7	30.251660779385624	32.23923268193337	18.274979477698107	19.234127060982896
8	28.616297629217723	34.67832332938836	17.746643359299647	18.958735682094265
9	28.321133524597737	15.84828145063025	17.715865113909572	38.11471991086245
10-11	27.36659089961213	25.507145240379174	25.170326920441617	21.955936939567085
12-13	26.49783338487505	22.7910399380009	26.218129519991358	24.49299715713269
14-15	24.172548926149407	23.99023487828601	23.7328875774723	28.104328618092282
16-17	24.157653987598472	27.03096210876582	24.354453363202648	24.456930540433056
18-19	24.116879093315298	26.17751936714385	26.292657242142546	23.4129442973983
20-21	24.70265978917704	25.222753806029175	26.47217848902764	23.602407915766143
22-23	24.49390722538574	24.64676653226467	26.417029979042823	24.442296263306766
24-25	24.072529413779904	24.904560681234912	26.382138585487265	24.640771319497922
26-27	23.65472638742629	25.17959572157785	26.56951691245798	24.596160978537885
28-29	23.767667258988723	25.832999438460817	26.29530109373534	24.104032208815124
30-31	24.67588613713174	25.3268321891538	26.26256946626967	23.73471220744479
32-33	24.199620476965723	25.36816564363264	26.38001605674376	24.052197822657885
34-35	24.169607175785597	25.568130193678883	26.60113141953233	23.661131211003188
36-37	24.19813098311063	25.333795572926366	26.772385975521658	23.69568746844135
38-39	24.11285745990655	25.77263769998317	26.454788648269435	23.65971619184085
40-41	24.140189672147507	25.573343422171714	26.430398186392285	23.856068719288498
42-43	23.887199140859945	25.581535638374724	26.166050264459635	24.365214956305696
44-45	23.438935965169673	25.79367680068636	26.68808062332339	24.079306610820577
46-47	23.50060101077053	25.546271871355398	26.227864259445997	24.725262858428078
48-49	23.697400386374706	25.987869562044118	25.930412336583906	24.384317714997266
50-51	23.734228121941882	25.990885787100687	26.13447299473166	24.14041309622577
52-53	23.960854611994296	25.824769984911427	25.978150614639638	24.236224788454635
54-55	23.373807846355728	25.503821296485242	26.614723050960055	24.507647806198975
56-57	23.598200095625504	25.749848444000246	25.964372796480028	24.68757866389422
58-59	23.609818147695233	25.83102585910282	26.23307748793882	24.326078505263126
60-61	24.234698057253166	25.69328491485308	26.285842807755493	23.786174220138253
62-63	23.856552804791402	25.878764137158548	26.204106832457263	24.06057622559278
64-65	23.651598450330592	25.38064015466904	26.180535592200414	24.787225802799952
66-67	23.59954064009509	25.729851988995616	26.013153720234328	24.657453650674967
68-69	23.382335198676138	25.623539365088348	26.301519730580353	24.69260570565516
70-71	23.430780986313042	25.49924110288083	26.38872959579605	24.681248315010077
72-73	23.534710420052164	26.042049901023134	25.859102818271325	24.56413686065338
74-75	23.354667850317785	25.99181672076012	25.91261288501554	24.740902543906554
76-77	23.27911327451819	25.379634746316853	26.159906102307374	25.181345876857584
78-79	23.13958493764234	25.448263175690265	26.48912148162933	24.923030405038062
80-81	23.503579998480717	25.73446941994641	25.91015522015464	24.851795361418237
82-83	23.597529823390715	25.766493537830907	26.06927040122496	24.56670623755342
84-85	23.478482027022398	25.927507823566476	25.734916268102936	24.85909388130819
86-87	22.969224077966068	26.199861774970245	25.91574082211124	24.91517332495245
88-89	22.89173316015485	25.699987637201005	26.13071202274755	25.2775671798966
90-91	23.379763408443655	25.352973332062295	26.290015312019637	24.97724794747441
92-93	23.420982860343152	25.567424374447956	26.05858643633065	24.953006328878242
94-95	23.456045375974597	25.517804305683097	25.79540892967126	25.230741388671053
96-97	22.972689666266614	25.439150806917382	26.027800751228742	25.56035877558726
98-99	22.69684134091734	25.365419525409845	26.081680149694186	25.856058983978624
100-101	22.81952994552921	25.674144918815138	26.081223589412083	25.42510154624357
102-103	22.680820830273664	25.58302513222982	26.049236708873956	25.68691732862256
104-105	22.88305685844893	25.577588479658726	25.882562346489042	25.656792315403305
106-107	22.932244313103993	25.404137522193494	26.077315272696943	25.586302892005573
108-109	22.780999647733392	25.344614305301207	26.094428304063428	25.779957742901978
110-111	22.6970494528328	25.840412173967444	25.9746532075601	25.487885165639657
112-113	22.571643272767624	25.869218583943592	26.000592822527853	25.558545320760928
114-115	22.489135979921578	25.994518650366604	25.841025965659643	25.67531940405218
116-117	22.33265635484695	25.80472186908621	25.891634288625198	25.970987487441636
118-119	22.31765761673003	25.40843874629022	26.165699114121548	26.1082045228582
120-121	22.291504606088996	25.53995264138416	26.23904892519625	25.929493827330592
122-123	22.27351587784207	25.662310657333766	25.940778481043296	26.12339498378087
124-125	21.940649539447815	25.564816911142625	25.804439591997237	26.69009395741233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	81.0
1	84.5
2	97.5
3	96.5
4	77.5
5	104.5
6	276.5
7	586.0
8	850.0
9	988.5
10	903.5
11	761.0
12	757.0
13	792.5
14	880.5
15	976.5
16	1018.5
17	1075.0
18	1118.5
19	1142.0
20	1256.0
21	1348.5
22	1464.0
23	1696.0
24	1891.5
25	2157.0
26	2699.5
27	3465.5
28	4139.0
29	4834.5
30	6145.0
31	7736.5
32	9719.0
33	12061.0
34	14623.5
35	17354.5
36	20076.0
37	23005.0
38	25520.5
39	28078.5
40	30611.0
41	32875.5
42	35010.5
43	37997.0
44	41424.5
45	44719.5
46	48445.0
47	51857.0
48	55715.0
49	59377.5
50	61957.5
51	63619.5
52	63421.0
53	61834.0
54	59861.0
55	56933.0
56	52515.5
57	47227.5
58	42793.0
59	38329.0
60	32872.5
61	27530.5
62	22151.0
63	16807.5
64	12577.5
65	9744.5
66	7383.5
67	5463.0
68	4181.5
69	3280.0
70	2641.5
71	2085.0
72	1584.5
73	1288.0
74	1045.5
75	769.0
76	572.0
77	376.5
78	251.0
79	201.0
80	177.5
81	146.5
82	114.5
83	103.5
84	93.0
85	90.0
86	88.0
87	76.5
88	76.0
89	75.5
90	70.5
91	66.5
92	59.5
93	56.5
94	54.0
95	51.0
96	42.5
97	36.0
98	23.0
99	8.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3323060790712708
2	0.0
3	0.0
4	0.016012058942250836
5	0.1698022994806135
6	0.0414079291715882
7	0.022044509055377894
8	0.007521943968220159
9	0.0070006211189375745
10-11	0.0019363420116210311
12-13	0.0583136844268949
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.4894938550931008E-4
92-93	2.9789877101862015E-4
94-95	7.447469275465504E-5
96-97	3.351361173959477E-4
98-99	3.723734637732752E-5
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.4894938550931008E-4
108-109	3.7237346377327516E-4
110-111	2.9789877101862015E-4
112-113	6.702722347918954E-4
114-115	2.2342407826396514E-4
116-117	5.213228492825853E-4
118-119	2.2342407826396514E-4
120-121	3.351361173959477E-4
122-123	0.002494902207280944
124-125	1.4894938550931008E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1342738.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.31692189145774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.98251990058367	55.694622124948026
2	8.981318362242911	11.912267766223565
3	2.7804804751378334	5.53178719471317
4	1.2947284122846825	3.434496119525375
5	0.7329405094213217	2.4303179257189513
6	0.483715161701358	1.9247100357757685
7	0.3299634720068771	1.531751326007999
8	0.23079722838334613	1.2244609413970637
9	0.17716654809959442	1.0574226118889993
>10	0.9468260858163676	11.07896305433022
>50	0.043241391398370986	1.926680749477922
>100	0.015515459662693073	1.5337759259837302
>500	3.3728282617021677E-4	0.18110266806206757
>1k	4.4971043489362234E-4	0.537641555947126
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2442	0.1818671997068676	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2064	0.153715765845608	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1640	0.12213849611763426	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	7.447469275465504E-5	1.4894938550931008E-4
14-15	0.0	0.0	0.0	7.447469275465504E-5	2.234240782639651E-4
16-17	0.0	0.0	0.0	1.4894938550931008E-4	3.3513611739594766E-4
18-19	0.0	0.0	0.0	2.6066142464129265E-4	3.723734637732752E-4
20-21	0.0	0.0	0.0	2.9789877101862015E-4	4.468481565279302E-4
22-23	0.0	0.0	0.0	4.840855029052578E-4	6.702722347918953E-4
24-25	0.0	0.0	0.0	8.192216203012054E-4	0.001005408352187843
26-27	0.0	0.0	0.0	0.0015267312014704284	0.0010426456985651706
28-29	0.0	0.0	0.0	0.006032450113127058	0.0010426456985651706
30-31	0.0	0.0	0.0	0.019326182769832983	0.001079883044942498
32-33	0.0	0.0	0.0	0.04230162548464406	0.0012288324304518081
34-35	0.0	0.0	0.0	0.07097438219518626	0.0012660697768291356
36-37	0.0	0.0	0.0	0.11122795362907731	0.0012660697768291356
38-39	0.0	0.0	0.0	0.1819789117459996	0.0013405444695837906
40-41	0.0	0.0	0.0	0.2730242236385654	0.0013405444695837906
42-43	0.0	0.0	0.0	0.35211634734400904	0.0013405444695837906
44-45	0.0	0.0	0.0	0.43820909216839027	0.0013405444695837906
46-47	0.0	0.0	0.0	0.5221420709028864	0.0013405444695837906
48-49	0.0	0.0	0.0	0.6161663705056384	0.0013405444695837906
50-51	0.0	0.0	0.0	0.7277666975984891	0.0013405444695837906
52-53	0.0	0.0	0.0	0.8397021608087356	0.0015267312014704284
54-55	0.0	0.0	0.0	0.9580796849422597	0.001712917933357066
56-57	0.0	0.0	0.0	1.0792127727077063	0.0017501552797343934
58-59	0.0	0.0	0.0	1.2163951567617808	0.001787392626111721
60-61	0.0	0.0	0.0	1.3638178110696204	0.001787392626111721
62-63	0.0	0.0	0.0	1.523305365603714	0.001787392626111721
64-65	0.0	0.0	0.0	1.6756061122869839	0.001787392626111721
66-67	0.0	0.0	0.0	1.8420570505936378	0.001787392626111721
68-69	0.0	0.0	0.0	2.0233656901048453	0.001787392626111721
70-71	0.0	0.0	0.0	2.225527243587357	0.001861867318866376
72-73	0.0	0.0	0.0	2.4130172825972007	0.0019363420116210311
74-75	0.0	0.0	0.0	2.598645454288178	0.0019363420116210311
76-77	0.0	0.0	0.0	2.8126112465723025	0.0019363420116210311
78-79	0.0	0.0	0.0	3.0629951636134525	0.0019363420116210311
80-81	0.0	0.0	0.0	3.3116661627212456	0.0019363420116210311
82-83	0.0	0.0	0.0	3.573966030603141	0.002085291397130341
84-85	0.0	0.0	0.0	3.8357818129821304	0.002085291397130341
86-87	0.0	0.0	0.0	4.116216268549784	0.002122528743507669
88-89	0.0	0.0	0.0	4.44036736876442	0.002159766089884996
90-91	0.0	0.0	0.0	4.7914038330634865	0.002159766089884996
92-93	0.0	0.0	0.0	5.122816215821702	0.002159766089884996
94-95	0.0	0.0	0.0	5.4684905022424335	0.002234240782639651
96-97	0.0	0.0	0.0	5.8454813969664965	0.0023459528217716335
98-99	0.0	0.0	0.0	6.268683838544824	0.0024576648609036162
100-101	0.0	0.0	0.0	6.725921214712029	0.0024576648609036162
102-103	0.0	0.0	0.0	7.20926197068974	0.002867275671054219
104-105	0.0	0.0	0.0	7.7098808553865315	0.0029789877101862017
106-107	0.0	0.0	0.0	8.239619344950391	0.003016225056563529
108-109	0.0	0.0	0.0	8.786487013847825	0.0031279370956955117
110-111	0.0	0.0	0.0	9.368320550993566	0.0032024117884501668
112-113	0.0	0.0	0.0	9.956819573140852	0.0032024117884501668
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1980	0.0	73.61674	1
GTATCAA	3175	0.0	46.155884	2
GTACATG	7045	0.0	41.040848	1
TACATGG	7045	0.0	40.587837	2
ACATGGG	7165	0.0	39.741787	3
CATGGGG	6195	0.0	36.444633	4
ATGGGGG	4195	0.0	35.643265	5
TCAACGC	4215	0.0	35.332806	5
CAACGCA	4315	0.0	34.51397	6
ATCAACG	4480	0.0	33.64172	4
TATCAAC	4505	0.0	33.190563	3
AACGCAG	4485	0.0	33.066757	7
TGGGGGG	3290	0.0	30.238222	6
GTGGTAT	920	0.0	26.622448	1
ACGCAGA	5710	0.0	25.86748	8
CGCAGAG	6015	0.0	24.555828	9
GAGTACA	4820	0.0	23.672182	1
AGTACAT	4650	0.0	23.444113	2
TGGTATC	875	0.0	23.147633	2
GTATAGG	445	0.0	22.821323	1
>>END_MODULE