FastQCFastQC Report
Mon 15 Aug 2016
SRR2921317_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921317_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913291
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC11090.12142898594204915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.056.4742771
GTATCAA19650.041.1862141
GTACATG57700.038.881331
TACATGG59350.036.9921722
ACATGGG61050.035.4747923
CATGGGG51200.034.5136344
TCAACGC23750.034.3210954
ATGGGGG32800.034.1026545
CAACGCA24600.032.6514745
ATCAACG25150.032.1740043
TGGGGGG25150.032.172246
TATCAAC25700.031.4854532
AACGCAG26300.031.2179036
GAGTACA41400.026.1605321
ACGCAGA32350.025.3782357
AGTACAT40600.025.2061542
CGCAGAG34700.024.0024288
TATGTAA5750.022.7645472
GTATAGT2653.718924E-822.4558581
CCTATAC2403.562327E-722.3118423
Produced by FastQC (version 0.11.5)