##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921317_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	913291
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.77292889122963	31.0	31.0	34.0	25.0	34.0
2	29.974782407797733	31.0	31.0	34.0	25.0	34.0
3	30.03412493936763	31.0	31.0	34.0	25.0	34.0
4	33.390503136459245	37.0	35.0	37.0	27.0	37.0
5	33.45424185719557	37.0	35.0	37.0	27.0	37.0
6	33.494305757967616	37.0	35.0	37.0	28.0	37.0
7	33.43944044121753	37.0	35.0	37.0	27.0	37.0
8	33.37587472120058	37.0	35.0	37.0	27.0	37.0
9	34.91768341087342	39.0	37.0	39.0	27.0	39.0
10-11	34.92209054945248	39.0	37.0	39.0	27.0	39.0
12-13	34.82670802624793	39.0	37.0	39.0	27.0	39.0
14-15	36.05564327251665	40.0	37.0	41.0	26.5	41.0
16-17	36.035137760034864	40.0	37.0	41.0	27.0	41.0
18-19	36.03867496778135	40.0	37.0	41.0	26.0	41.0
20-21	36.077941751314754	40.0	37.0	41.0	26.0	41.0
22-23	36.04587420657819	40.0	37.0	41.0	26.0	41.0
24-25	36.06331443099735	40.0	37.0	41.0	26.0	41.0
26-27	36.053699204306184	40.0	37.0	41.0	27.0	41.0
28-29	35.989691675490064	40.0	37.0	41.0	27.0	41.0
30-31	35.90790449046361	40.0	36.0	41.0	27.0	41.0
32-33	35.81786637555828	40.0	36.0	41.0	26.5	41.0
34-35	35.699088242411236	40.0	36.0	41.0	25.5	41.0
36-37	35.586920817132764	40.0	36.0	41.0	25.0	41.0
38-39	35.47530852707406	40.0	35.0	41.0	25.0	41.0
40-41	35.36723508717375	40.0	35.0	41.0	24.0	41.0
42-43	35.167993005515214	40.0	35.0	41.0	23.5	41.0
44-45	34.93310237372316	40.0	35.0	41.0	23.0	41.0
46-47	34.70912118919381	39.5	35.0	41.0	22.0	41.0
48-49	34.49464902205321	39.0	35.0	41.0	19.5	41.0
50-51	34.32171290421125	39.0	34.0	41.0	19.0	41.0
52-53	34.13908546126043	39.0	34.0	41.0	18.0	41.0
54-55	33.939385146683804	38.5	34.0	40.0	15.5	41.0
56-57	33.69993189465351	38.0	34.0	40.0	13.5	41.0
58-59	33.43913823742925	38.0	33.0	40.0	10.5	41.0
60-61	33.15234355752986	37.0	33.0	40.0	8.0	41.0
62-63	32.81467297936803	37.0	33.0	40.0	7.0	41.0
64-65	32.4780590195239	36.0	32.5	39.0	4.0	41.0
66-67	32.147293140959455	36.0	32.0	39.0	2.0	40.5
68-69	31.79347984377378	35.0	32.0	38.5	2.0	40.0
70-71	31.746648111062083	35.0	32.0	38.5	2.0	40.0
72-73	31.45015717881814	35.0	32.0	37.0	2.0	39.5
74-75	31.12337305415251	35.0	32.0	37.0	2.0	39.0
76-77	30.816554088455923	35.0	32.0	36.5	2.0	39.0
78-79	30.519825553958157	35.0	31.5	36.0	2.0	37.5
80-81	30.239802538292835	35.0	31.0	35.5	2.0	37.0
82-83	30.00388539906777	35.0	31.0	35.0	2.0	37.0
84-85	29.778739197035776	35.0	31.0	35.0	2.0	36.0
86-87	29.600025621625527	35.0	31.0	35.0	2.0	36.0
88-89	29.457581975514927	35.0	31.0	35.0	2.0	36.0
90-91	29.331081221647864	35.0	31.0	35.0	2.0	35.0
92-93	29.21101543757685	35.0	31.0	35.0	2.0	35.0
94-95	29.083390726504476	34.0	30.0	35.0	2.0	35.0
96-97	28.983826075150198	34.0	30.0	35.0	2.0	35.0
98-99	28.90043808599888	34.0	30.0	35.0	2.0	35.0
100-101	28.808033803026635	34.0	30.0	35.0	2.0	35.0
102-103	27.98924384451396	33.5	28.0	34.5	2.0	35.0
104-105	28.575148008685073	34.0	29.5	35.0	2.0	35.0
106-107	28.64457166445306	34.0	30.0	35.0	2.0	35.0
108-109	28.5869585926063	34.0	30.0	35.0	2.0	35.0
110-111	28.51097788109157	34.0	29.0	35.0	2.0	35.0
112-113	28.423642628691184	34.0	29.0	35.0	2.0	35.0
114-115	28.287331748588347	34.0	29.0	35.0	2.0	35.0
116-117	28.158890211334615	34.0	29.0	35.0	2.0	35.0
118-119	28.1583219368197	34.0	29.0	35.0	2.0	35.0
120-121	28.320501351704987	34.0	30.0	35.0	2.0	35.0
122-123	28.190736577936278	34.5	30.0	35.0	2.0	35.0
124-125	27.612963447575858	34.0	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40794.0
3	6169.0
4	3769.0
5	3241.0
6	3176.0
7	3717.0
8	3738.0
9	3916.0
10	4422.0
11	5001.0
12	4961.0
13	5066.0
14	5224.0
15	4873.0
16	3819.0
17	3483.0
18	3080.0
19	2847.0
20	2759.0
21	2728.0
22	3021.0
23	4244.0
24	6439.0
25	8166.0
26	8041.0
27	8333.0
28	10227.0
29	12206.0
30	14639.0
31	18567.0
32	23672.0
33	32703.0
34	51580.0
35	82113.0
36	165753.0
37	274768.0
38	71985.0
39	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.986718312805195	18.572788092038763	12.67936793712225	24.76112565803379
2	16.063479478786697	20.012002054366242	39.47777922571418	24.44673924113288
3	19.616114096987697	25.257559232052984	28.352978795165946	26.773347875793373
4	13.151917753013736	15.686764087468461	35.266308347350716	35.89500981216709
5	13.75717112474554	39.22237029558339	32.07834772029684	14.94211085937423
6	32.652799698579095	34.86329269481462	17.766199456960447	14.717708149645842
7	28.865023150277498	33.85492638781204	19.613234862521956	17.6668155993885
8	27.166085539572126	35.917487253166996	18.91930072013036	17.997126487130515
9	28.344223501958478	16.96862383090837	18.084031147305822	36.60312151982733
10-11	26.236520872628923	26.79701166480055	26.18839433103286	20.778073131537667
12-13	25.509496233426283	24.30306477937388	26.548226324022252	23.639212663177588
14-15	23.619801190927227	25.472713575212598	23.9642897032318	26.943195530628376
16-17	23.802836564914962	28.153604835464453	24.69119985984161	23.35235873977898
18-19	23.77748954675885	27.698885334364103	25.97451801757163	22.549107101305417
20-21	24.770324250693136	26.534365405083417	25.77805055384979	22.917259790373656
22-23	24.59768529088258	25.722573259245994	25.909762588742467	23.76997886112895
24-25	24.17598782363988	25.67213617375403	25.942326538880582	24.20954946372551
26-27	23.642343243484433	26.11771842600455	26.03154319902809	24.20839513148292
28-29	23.764730487647302	26.926926269269263	25.770989507709896	23.537353735373536
30-31	24.981453508393965	26.01857879398256	25.88258031383675	23.117387383786724
32-33	24.532232614011285	25.988561366854245	25.730244924284484	23.74896109484999
34-35	24.31071203701169	26.399627693065785	25.9888307919735	23.300829477949026
36-37	24.123975272398372	26.454876397579802	25.957521129660037	23.463627200361792
38-39	24.26171472497101	26.672933792216863	25.772175933580883	23.293175549231247
40-41	24.25287167526253	26.12255401158525	25.984417945095977	23.64015636805624
42-43	23.94766263445423	26.28327615526883	25.57726484813776	24.19179636213918
44-45	23.4295979269176	26.432238555587617	26.25282034800672	23.885343169488063
46-47	23.379993123128845	26.196936945223747	25.984991338208463	24.438078593438938
48-49	23.471658816244272	26.481227699115383	25.944829315592454	24.10228416904789
50-51	23.524574457018655	26.59050966608084	26.090315304217377	23.794600572683127
52-53	23.821344569468838	26.390154219463803	25.86170837147575	23.92679283959161
54-55	23.296526531448023	26.101603417294744	26.44149328159065	24.16037676966658
56-57	23.64241111880665	26.333087866933486	25.805289481536636	24.219211532723225
58-59	23.504487359181457	26.60375457715017	26.10212957934019	23.789628484328187
60-61	24.265876959144244	26.52046346589842	25.992513546407636	23.2211460285497
62-63	24.034814354104707	26.436746367892916	25.96962038622756	23.558818891774816
64-65	23.6686704771916	26.02769200446751	26.000646037273068	24.302991481067824
66-67	23.7168151283048	26.318060125594712	25.829040081904836	24.13608466419565
68-69	23.351698439230624	26.263159940672477	26.272358006231688	24.112783613865208
70-71	23.434492198193265	26.25688475225842	26.32198193265809	23.98664111689023
72-73	23.358233306396688	26.91042141616681	25.752638367858232	23.978706909578275
74-75	23.425089076690266	26.719667388624025	25.80234720090051	24.052896333785196
76-77	23.144311129725896	26.063985589965533	26.122457918264665	24.669245362043903
78-79	23.087914933641823	26.188443970442265	26.386415311681677	24.33722578423423
80-81	23.47011568950254	26.48315628512472	25.835971764994444	24.210756260378304
82-83	23.713915136822315	26.435414753847947	26.17310879373751	23.67756131559223
84-85	23.606054915169974	26.704361599788	25.62826561513608	24.061317869905942
86-87	23.05540605575482	27.064689582750468	25.83493433475995	24.044970026734763
88-89	23.019457357677798	26.36721348453633	26.145369275891085	24.467959881894785
90-91	23.649026716700483	26.07733487800151	26.238298645823864	24.03533975947414
92-93	23.706697604998606	26.296122104938107	26.039884386417427	23.957295903645857
94-95	23.60186589432074	26.314858716540652	25.877567110327572	24.205708278811038
96-97	23.167955357035307	26.106465060813555	26.166638012879968	24.558941569271166
98-99	22.857424430732507	26.21472019797752	26.05194719869475	24.875908172595224
100-101	22.88123204486136	26.55572875346236	26.146623982234164	24.416415219442115
102-103	22.738451632966363	26.468881330988587	26.238439588019002	24.554227448026055
104-105	22.992886957816683	26.551324278512396	25.934079531169928	24.521709232500996
106-107	23.02811464863533	26.33956582441348	26.224862438062907	24.407457088888282
108-109	22.89010910193889	26.40105953767321	26.15741843361218	24.551412926775722
110-111	22.657069130620698	27.060441591050573	26.136896839262807	24.145592439065922
112-113	22.641768719997195	27.0825120887207	26.055189827954727	24.220529363327376
114-115	22.774194502067022	27.357283521147345	25.858658524500683	24.009863452284947
116-117	22.55334851332611	27.099243537051464	25.944538460148685	24.402869489473737
118-119	22.52789041786961	26.664199094675254	26.292527917835333	24.5153825696198
120-121	22.446923550749435	26.75206351379098	26.524782697967286	24.2762302374923
122-123	22.363436084758977	26.979202252998014	26.20024483395965	24.45711682828336
124-125	22.263788160076736	26.80711002004326	26.127577196122303	24.801524623757697
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	672.0
1	583.0
2	434.5
3	323.0
4	252.5
5	221.5
6	223.0
7	265.5
8	326.5
9	355.5
10	364.0
11	387.5
12	402.0
13	444.5
14	486.0
15	542.0
16	591.0
17	609.5
18	655.5
19	708.0
20	787.5
21	862.0
22	1000.0
23	1189.0
24	1352.5
25	1644.0
26	2163.5
27	2828.0
28	3377.0
29	4176.5
30	5526.0
31	6730.5
32	8288.5
33	10161.5
34	11968.5
35	13680.5
36	15720.5
37	17663.0
38	18803.5
39	20486.5
40	22010.5
41	23230.0
42	24502.5
43	26351.0
44	28885.0
45	31108.0
46	33546.0
47	36052.5
48	38618.0
49	41034.0
50	42466.5
51	43147.5
52	42244.5
53	40704.5
54	39645.0
55	37634.5
56	34886.5
57	31521.0
58	27873.0
59	24069.0
60	19875.5
61	16439.0
62	13461.5
63	10147.0
64	7220.0
65	5303.5
66	3931.0
67	2808.5
68	2074.5
69	1536.0
70	1127.0
71	830.5
72	597.5
73	443.5
74	322.0
75	212.5
76	137.5
77	84.5
78	64.0
79	45.5
80	31.0
81	21.0
82	23.0
83	22.5
84	15.5
85	16.5
86	9.5
87	5.5
88	6.5
89	3.5
90	4.0
91	3.5
92	1.0
93	3.0
94	3.0
95	1.0
96	1.5
97	1.5
98	1.5
99	1.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016862095432890502
2	0.012482330385386476
3	0.011277894997322869
4	0.015219683540076493
5	0.008759530095008053
6	0.030877343584903388
7	0.013905754025825284
8	0.013467777521074881
9	0.008978518347383254
10-11	0.00804781827478865
12-13	0.0066243946343498405
14-15	0.005803188687942835
16-17	0.0042155238582226254
18-19	0.006295912255787038
20-21	0.005146223930817232
22-23	0.004872488615348229
24-25	0.005036729804629631
26-27	0.003887041479659824
28-29	0.005365212183192432
30-31	0.00498198274153583
32-33	0.007664588833132048
34-35	0.007226612328381644
36-37	0.0066243946343498405
38-39	0.0065696475712560395
40-41	0.006679141697443641
42-43	0.006788635823631241
44-45	0.006733888760537441
46-47	0.009307000725946058
48-49	0.007719335896225847
50-51	0.005036729804629631
52-53	0.005146223930817232
54-55	0.0059126828141304365
56-57	0.006952877012912642
58-59	0.006460153445068439
60-61	0.004544006236785428
62-63	0.005146223930817232
64-65	0.0033943179118156204
66-67	0.003941788542753624
68-69	0.006076924003411837
70-71	0.004489259173691627
72-73	0.004051282668941224
74-75	0.003613306164190822
76-77	0.0042155238582226254
78-79	0.0030110884701590184
80-81	0.0030110884701590184
82-83	0.004872488615348229
84-85	0.009033265410477056
86-87	0.0066243946343498405
88-89	0.004106029732035025
90-91	0.004598753299879228
92-93	0.00837630065335145
94-95	0.006131671066505638
96-97	0.009854471356884059
98-99	0.006131671066505638
100-101	0.008485794779539051
102-103	0.004927235678442029
104-105	0.004270270921316426
106-107	0.007226612328381644
108-109	0.006843382886725042
110-111	0.007719335896225847
112-113	0.004708247426066829
114-115	0.0022446295868458134
116-117	0.0030110884701590184
118-119	0.16024465367555357
120-121	0.011332642060416669
122-123	0.0020803883975644127
124-125	0.001806653082095411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	913291.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.81551906498692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.28661780136478	57.1594073643172
2	8.728258112006205	11.838227087977673
3	2.7323665378001216	5.558905651101494
4	1.2782676944515983	3.4674554881295694
5	0.7466849709136893	2.531841444026825
6	0.5004809251124767	2.036422423117647
7	0.3402913394068221	1.6153923670635295
8	0.24957060675743198	1.3539808192495184
9	0.18139099517914076	1.1071012042609174
>10	0.9150740669252341	10.520423831703837
>50	0.0313146229854294	1.4445422244502868
>100	0.009036838741747193	1.0031937039430345
>500	4.8411626649857146E-4	0.24174262114664263
>1k	1.6137208883285715E-4	0.12136376951182737
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1109	0.12142898594204915	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	5.4747063093800336E-5	0.0
12-13	0.0	0.0	0.0	1.0949412618760067E-4	0.0
14-15	0.0	0.0	0.0	1.0949412618760067E-4	5.4747063093800336E-5
16-17	0.0	0.0	0.0	1.0949412618760067E-4	1.0949412618760067E-4
18-19	0.0	0.0	0.0	1.0949412618760067E-4	1.0949412618760067E-4
20-21	0.0	0.0	0.0	1.6424118928140102E-4	1.0949412618760067E-4
22-23	0.0	0.0	0.0	2.7373531546900166E-4	1.0949412618760067E-4
24-25	0.0	0.0	0.0	6.022176940318037E-4	1.0949412618760067E-4
26-27	0.0	0.0	0.0	0.0014234236404388088	1.0949412618760067E-4
28-29	0.0	0.0	0.0	0.0038322944165660234	1.0949412618760067E-4
30-31	0.0	0.0	0.0	0.013029801016324479	1.0949412618760067E-4
32-33	0.0	0.0	0.0	0.03383368499196861	1.0949412618760067E-4
34-35	0.0	0.0	0.0	0.060002781150805165	1.0949412618760067E-4
36-37	0.0	0.0	0.0	0.0971212899284018	1.0949412618760067E-4
38-39	0.0	0.0	0.0	0.16013515954936597	1.0949412618760067E-4
40-41	0.0	0.0	0.0	0.23924466571990746	1.0949412618760067E-4
42-43	0.0	0.0	0.0	0.3059813356312501	1.0949412618760067E-4
44-45	0.0	0.0	0.0	0.3775904941579409	1.0949412618760067E-4
46-47	0.0	0.0	0.0	0.45138953520838376	1.0949412618760067E-4
48-49	0.0	0.0	0.0	0.5373424242656503	3.28482378562802E-4
50-51	0.0	0.0	0.0	0.6302481903358295	3.28482378562802E-4
52-53	0.0	0.0	0.0	0.7355815397283012	3.28482378562802E-4
54-55	0.0	0.0	0.0	0.8476487778813105	3.28482378562802E-4
56-57	0.0	0.0	0.0	0.9512849683178746	3.28482378562802E-4
58-59	0.0	0.0	0.0	1.081145001976369	3.28482378562802E-4
60-61	0.0	0.0	0.0	1.213030676969334	3.28482378562802E-4
62-63	0.0	0.0	0.0	1.379516495837581	4.379765047504027E-4
64-65	0.0	0.0	0.0	1.5177528301494267	4.379765047504027E-4
66-67	0.0	0.0	0.0	1.6737272129036638	4.379765047504027E-4
68-69	0.0	0.0	0.0	1.8488083206776373	4.379765047504027E-4
70-71	0.0	0.0	0.0	2.0403135473797507	4.379765047504027E-4
72-73	0.0	0.0	0.0	2.215175666901349	6.56964757125604E-4
74-75	0.0	0.0	0.0	2.398633075328674	6.56964757125604E-4
76-77	0.0	0.0	0.0	2.622931792823974	6.56964757125604E-4
78-79	0.0	0.0	0.0	2.8726331475947973	6.56964757125604E-4
80-81	0.0	0.0	0.0	3.0977530710365047	7.117118202194043E-4
82-83	0.0	0.0	0.0	3.339954078163477	7.664588833132046E-4
84-85	0.0	0.0	0.0	3.595732356937712	7.664588833132046E-4
86-87	0.0	0.0	0.0	3.8741759198327808	7.664588833132046E-4
88-89	0.0	0.0	0.0	4.190285462136384	7.664588833132046E-4
90-91	0.0	0.0	0.0	4.523421341062159	7.664588833132046E-4
92-93	0.0	0.0	0.0	4.833837188804006	7.664588833132046E-4
94-95	0.0	0.0	0.0	5.146552413195794	7.664588833132046E-4
96-97	0.0	0.0	0.0	5.514069447744475	7.664588833132046E-4
98-99	0.0	0.0	0.0	5.930694597888296	7.664588833132046E-4
100-101	0.0	0.0	0.0	6.363415384581694	7.664588833132046E-4
102-103	0.0	0.0	0.0	6.804512471928444	8.759530095008054E-4
104-105	0.0	0.0	0.0	7.273913790894687	8.759530095008054E-4
106-107	0.0	0.0	0.0	7.7910545488787255	8.759530095008054E-4
108-109	0.0	0.0	0.0	8.325057402295654	8.759530095008054E-4
110-111	0.0	0.0	0.0	8.914957007131353	0.0010401941987822063
112-113	0.0	0.0	0.0	9.476114403842805	0.0010949412618760067
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1275	0.0	56.474277	1
GTATCAA	1965	0.0	41.186214	1
GTACATG	5770	0.0	38.88133	1
TACATGG	5935	0.0	36.992172	2
ACATGGG	6105	0.0	35.474792	3
CATGGGG	5120	0.0	34.513634	4
TCAACGC	2375	0.0	34.321095	4
ATGGGGG	3280	0.0	34.102654	5
CAACGCA	2460	0.0	32.651474	5
ATCAACG	2515	0.0	32.174004	3
TGGGGGG	2515	0.0	32.17224	6
TATCAAC	2570	0.0	31.485453	2
AACGCAG	2630	0.0	31.217903	6
GAGTACA	4140	0.0	26.160532	1
ACGCAGA	3235	0.0	25.378235	7
AGTACAT	4060	0.0	25.206154	2
CGCAGAG	3470	0.0	24.002428	8
TATGTAA	575	0.0	22.764547	2
GTATAGT	265	3.718924E-8	22.455858	1
CCTATAC	240	3.562327E-7	22.311842	3
>>END_MODULE