FastQCFastQC Report
Mon 15 Aug 2016
SRR2921317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913291
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27150.29727655259933583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22530.24669026630066432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21350.23376995941052742No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC10000.10949412618760067No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.10204852560684381No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.074.8728561
GTATCAA34350.049.2490231
GTACATG58250.040.0227171
TACATGG61250.037.661892
ACATGGG62350.036.4238433
CATGGGG52950.036.2482574
TCAACGC47150.034.3862234
ATGGGGG34600.034.282645
TATCAAC49000.033.93952
GTGGTAT8500.033.7567181
ATCAACG48300.033.5675053
TGGGGGG28050.033.5606966
CAACGCA48250.033.3552135
AACGCAG49750.032.694626
TGGTATC8900.030.138372
ACGCAGA57600.027.510247
CGCAGAG59000.026.7491578
GAGTACT33600.024.26876412-13
AGTACTT34400.024.13693612-13
AGAGTAC60550.024.04745110-11
Produced by FastQC (version 0.11.5)