##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921317_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 913291 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.8720385944896 31.0 30.0 34.0 16.0 34.0 2 29.701215713283062 33.0 31.0 34.0 25.0 34.0 3 29.943703595020644 33.0 31.0 34.0 26.0 34.0 4 31.92201171368162 37.0 33.0 37.0 19.0 37.0 5 31.600565427667632 37.0 33.0 37.0 19.0 37.0 6 31.77247339566469 37.0 33.0 37.0 17.0 37.0 7 32.00064930016829 37.0 35.0 37.0 17.0 37.0 8 32.276733264644015 37.0 35.0 37.0 17.0 37.0 9 33.98742240972483 39.0 35.0 39.0 17.0 39.0 10-11 34.207197924867316 39.0 37.0 39.0 17.0 39.0 12-13 34.25949177206389 39.0 37.0 39.0 17.5 39.0 14-15 35.60393182457727 40.0 37.0 41.0 18.0 41.0 16-17 35.65020513724541 40.0 37.5 41.0 19.5 41.0 18-19 35.70161646178491 40.5 38.0 41.0 21.0 41.0 20-21 35.697134319729415 41.0 38.0 41.0 20.5 41.0 22-23 35.655291686877455 41.0 37.5 41.0 19.5 41.0 24-25 35.5972291416427 41.0 37.0 41.0 19.0 41.0 26-27 35.49634946583291 40.0 37.0 41.0 18.0 41.0 28-29 35.41211125479174 40.0 37.0 41.0 17.5 41.0 30-31 35.27459320194768 40.0 36.5 41.0 16.5 41.0 32-33 35.09996594732675 40.0 35.5 41.0 15.5 41.0 34-35 35.004175011031535 40.0 35.0 41.0 14.0 41.0 36-37 34.91918621775535 40.0 35.0 41.0 9.0 41.0 38-39 34.81803609145388 40.0 35.0 41.0 8.5 41.0 40-41 34.66528357336271 40.0 35.0 41.0 5.0 41.0 42-43 34.50086281371436 40.0 35.0 41.0 2.0 41.0 44-45 34.3018413627201 40.0 35.0 41.0 2.0 41.0 46-47 34.01312779825926 40.0 35.0 41.0 2.0 41.0 48-49 33.724173894191445 40.0 35.0 41.0 2.0 41.0 50-51 33.5254677862806 39.5 35.0 41.0 2.0 41.0 52-53 33.35516609711472 39.0 34.0 41.0 2.0 41.0 54-55 33.168388279310754 39.0 34.0 41.0 2.0 41.0 56-57 32.938838770994124 39.0 34.0 41.0 2.0 41.0 58-59 32.882419185122814 38.5 34.0 41.0 2.0 41.0 60-61 32.65252641272059 38.0 34.0 41.0 2.0 41.0 62-63 32.36405099798421 37.0 34.0 40.0 2.0 41.0 64-65 32.06928514569836 37.0 33.0 40.0 2.0 41.0 66-67 31.762795757321598 36.0 33.0 39.0 2.0 41.0 68-69 31.42731670409541 36.0 33.0 39.0 2.0 41.0 70-71 31.09664389553822 35.0 33.0 38.5 2.0 40.5 72-73 30.749902276492378 35.0 32.5 37.0 2.0 39.5 74-75 30.41319962640604 35.0 32.0 37.0 2.0 39.0 76-77 30.111739303245077 35.0 32.0 36.5 2.0 39.0 78-79 29.853248854965173 35.0 32.0 36.0 2.0 38.0 80-81 29.621837399032728 35.0 32.0 35.5 2.0 37.0 82-83 29.41497124136776 35.0 31.0 35.0 2.0 37.0 84-85 29.24148053577666 35.0 31.0 35.0 2.0 36.0 86-87 29.09263860040228 35.0 31.0 35.0 2.0 36.0 88-89 28.96113232255656 35.0 31.0 35.0 2.0 36.0 90-91 28.854571544009524 35.0 31.0 35.0 2.0 35.5 92-93 28.78000878142892 35.0 31.0 35.0 2.0 35.0 94-95 28.704865152508894 35.0 31.0 35.0 2.0 35.0 96-97 28.635808849534264 35.0 31.0 35.0 2.0 35.0 98-99 28.584001156257973 35.0 31.0 35.0 2.0 35.0 100-101 28.530891030350674 35.0 31.0 35.0 2.0 35.0 102-103 27.887628368176188 34.0 29.0 35.0 2.0 35.0 104-105 28.398312257538944 35.0 30.0 35.0 2.0 35.0 106-107 28.447593373853458 35.0 30.0 35.0 2.0 35.0 108-109 28.51090889979207 35.0 30.5 35.0 2.0 35.0 110-111 28.610470813793192 35.0 31.0 35.0 2.0 35.0 112-113 28.570157813884073 35.0 31.0 35.0 2.0 35.0 114-115 28.50580154627605 35.0 31.0 35.0 2.0 35.0 116-117 28.44099909010381 35.0 31.0 35.0 2.0 35.0 118-119 28.364479120017606 35.0 31.0 35.0 2.0 35.0 120-121 28.262407053173632 35.0 31.0 35.0 2.0 35.0 122-123 28.146903889340855 35.0 31.0 35.0 2.0 35.0 124-125 27.64550510187881 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68529.0 3 3633.0 4 2824.0 5 2873.0 6 3067.0 7 3166.0 8 3396.0 9 3270.0 10 3613.0 11 3765.0 12 4434.0 13 5981.0 14 7934.0 15 6822.0 16 4118.0 17 3444.0 18 2947.0 19 2600.0 20 2304.0 21 2422.0 22 2609.0 23 3203.0 24 4130.0 25 5535.0 26 6403.0 27 6868.0 28 7965.0 29 9651.0 30 11734.0 31 14252.0 32 18575.0 33 24484.0 34 38316.0 35 63954.0 36 135521.0 37 304514.0 38 114189.0 39 246.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40716876518399 18.490320156204845 12.508804103343966 25.5937069752672 2 16.155529836601914 20.176154150210614 38.75654090536313 24.91177510782434 3 19.070263475715844 24.66081457060236 29.217412631899364 27.051509321782437 4 13.751842595776651 16.748949200209392 31.44273332793058 38.05647487608338 5 13.849841482277828 38.645194554450015 31.823776561317285 15.681187401954869 6 32.661992660349455 35.141260886235415 16.71063153858794 15.48611491482719 7 29.260928040093575 33.06329019652511 18.816862782893644 18.858918980487676 8 28.109915813264884 35.856343790242754 17.58416483429843 18.44957556219394 9 28.771428414995377 16.438735675607603 17.737772594624484 37.05206331477253 10-11 27.078848533292454 26.326989827761775 25.038761811950465 21.555399826995302 12-13 26.572425232426415 23.883991664858968 26.147451361910335 23.396131740804286 14-15 24.605903266319277 24.75919504298192 24.172251779553285 26.46264991114552 16-17 23.768054212731755 27.651975109795234 24.82084023602554 23.759130441447468 18-19 23.698963419107383 26.372426751166934 27.185365891046775 22.74324393867891 20-21 24.624900497212828 25.374004561525297 27.23354330656932 22.767551634692555 22-23 24.383246960716793 24.68572448431004 27.27318018024923 23.65784837472394 24-25 23.88318728641802 24.985847884190253 27.17085791932692 23.96010691006481 26-27 23.306153241409362 25.199963647950106 27.55753642595843 23.9363466846821 28-29 23.423804679997943 25.938830011464038 27.3176347954814 23.319730513056626 30-31 24.51819847124301 25.248798028229775 27.254347190544966 22.978656309982252 32-33 24.112303745465574 25.357963672038814 27.20830491048308 23.321427672012536 34-35 23.953865744872115 25.684967879898085 27.445961911373264 22.915204463856536 36-37 23.974724375910856 25.51032474862886 27.449246735158894 23.065704140301392 38-39 23.81218034558536 25.779789793176544 27.29250589352134 23.115523967716754 40-41 23.852255195770024 25.45771282099572 27.345336809406863 23.3446951738274 42-43 23.636168537738794 25.643360111946794 26.895096962523446 23.82537438779097 44-45 23.20295502747755 25.750883343863016 27.4430055699662 23.60315605869323 46-47 23.320715960192313 25.648944312382362 26.760692922628166 24.26964680479716 48-49 23.482657772823774 26.0980892180039 26.350911155371072 24.068341853801254 50-51 23.531656394292728 26.278480790897973 26.49927569635527 23.690587118454033 52-53 23.71779640881165 26.06836156274397 26.279083008592004 23.93475901985238 54-55 23.210838604563058 25.710917987804542 26.918090729022843 24.160152678609556 56-57 23.490158120467626 25.894922866862807 26.353155784957917 24.26176322771165 58-59 23.44028354598918 26.067211874418998 26.623661023704383 23.868843555887445 60-61 24.058104153002713 25.982463420749795 26.76408724053998 23.195345185707513 62-63 23.881709115714486 25.865797429296904 26.61610592899744 23.636387525991168 64-65 23.496563526849602 25.46986666900254 26.71985161356019 24.313718190587664 66-67 23.45807634149466 25.77573851050761 26.58139629099597 24.184788857001767 68-69 23.16994254843199 25.811488342707854 26.83427297542623 24.18429613343392 70-71 23.41745402067906 25.719075300205517 26.78653353640844 24.07693714270698 72-73 23.321318177886347 26.41474623093844 26.121192478629485 24.142743112545727 74-75 23.303142152939206 26.283736508954973 26.286473862109666 24.126647475996148 76-77 23.19715183878961 25.577554142108045 26.430239649793986 24.79505436930836 78-79 23.074189935080934 25.826653279184836 26.677313145536306 24.42184364019792 80-81 23.481343843309528 26.016899323435794 26.14276282148844 24.35899401176624 82-83 23.50762243359455 26.01914395302264 26.443652680251965 24.02958093313084 84-85 23.383346600371624 26.29474066863683 25.934723981732 24.387188749259543 86-87 23.007453265169588 26.568092754664175 26.024892394647487 24.399561585518743 88-89 22.79339224847283 26.06206565048818 26.31778918219932 24.82675291883967 90-91 23.373143725278183 25.729685340048995 26.439976458672653 24.45719447600016 92-93 23.44339731103147 25.9428854237496 26.21820960990357 24.39550765531536 94-95 23.351029438091643 25.95546100113929 25.88730079564037 24.806208765128694 96-97 22.959911309417897 25.77485184019271 26.187153552413672 25.078083297975716 98-99 22.71374770678114 25.819221813869735 26.06054700010566 25.406483479243462 100-101 22.812882202934222 26.160336628741547 26.137342862242157 24.88943830608207 102-103 22.664462914886933 26.04438234910888 26.049638067165887 25.241516668838297 104-105 22.950486701923815 26.016708821951408 25.88766985294923 25.14513462317555 106-107 23.056611872036125 25.77519273745769 26.121304428280645 25.046890962225536 108-109 22.95309303269018 25.76732493034771 26.078454022916137 25.201128014045977 110-111 22.628769832905665 26.408908923979496 25.985985777737874 24.976335465376962 112-113 22.701742994652815 26.42385806394393 26.05579093918708 24.818608002216177 114-115 22.751172413491048 26.424880678253388 25.917756411730373 24.906190496525188 116-117 22.478470575997637 26.264583344739044 25.995226024734887 25.261720054528432 118-119 22.495380698848937 25.87345167868825 26.24157234167773 25.389595280785077 120-121 22.47761118854779 25.99768310428987 26.433579220642713 25.091126486519634 122-123 22.399337769373368 26.222712276779113 26.03197058504703 25.345979368800485 124-125 22.088943319725015 26.15813496158666 25.845035993515747 25.907885725172576 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 48.0 1 45.0 2 68.0 3 86.5 4 74.0 5 95.0 6 211.0 7 642.5 8 951.0 9 1056.0 10 1071.5 11 867.0 12 833.5 13 918.0 14 961.0 15 1034.0 16 1103.0 17 1124.0 18 1193.5 19 1278.5 20 1337.0 21 1403.0 22 1524.0 23 1663.0 24 1759.0 25 1953.5 26 2435.5 27 3036.5 28 3480.5 29 4079.5 30 5180.0 31 6324.0 32 7777.0 33 9448.5 34 10895.0 35 12489.0 36 14498.5 37 16519.5 38 17891.5 39 19354.0 40 21109.0 41 22352.0 42 23548.0 43 25530.5 44 27693.5 45 29867.0 46 32417.5 47 35198.0 48 38044.5 49 40605.0 50 42230.5 51 43351.5 52 43208.5 53 41623.0 54 40091.0 55 38365.0 56 35786.5 57 32286.5 58 28456.5 59 24320.0 60 20137.0 61 16680.0 62 13662.0 63 10402.0 64 7474.0 65 5433.5 66 4049.0 67 2963.5 68 2167.0 69 1659.5 70 1241.0 71 937.0 72 695.5 73 535.0 74 408.5 75 294.5 76 236.0 77 177.5 78 141.5 79 117.5 80 92.0 81 88.5 82 89.0 83 82.5 84 76.5 85 66.0 86 58.5 87 66.0 88 75.0 89 61.0 90 48.5 91 54.5 92 49.5 93 47.0 94 42.5 95 37.5 96 31.0 97 22.5 98 16.5 99 4.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35081918030507253 2 0.0 3 0.0 4 0.01938046033520532 5 0.18843939116886074 6 0.04828690964873189 7 0.02485516664458535 8 0.009088012473570856 9 0.00832155359025765 10-11 0.002299376649939614 12-13 0.05792239275324076 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.8322944165660236E-4 92-93 4.379765047504027E-4 94-95 1.64241189281401E-4 96-97 3.8322944165660236E-4 98-99 5.4747063093800336E-5 100-101 0.0 102-103 0.0 104-105 1.0949412618760067E-4 106-107 2.1898825237520134E-4 108-109 4.92723567844203E-4 110-111 4.92723567844203E-4 112-113 7.117118202194043E-4 114-115 4.379765047504027E-4 116-117 6.56964757125604E-4 118-119 3.8322944165660236E-4 120-121 0.0 122-123 0.0015329177666264094 124-125 1.64241189281401E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 913291.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.39479031923145 #Duplication Level Percentage of deduplicated Percentage of total 1 85.39772057271345 58.407591923110544 2 8.006708399074174 10.95234284203815 3 2.5712603658952355 5.275824407446649 4 1.2190215168316738 3.334988841533352 5 0.6966702996376878 2.38243095326779 6 0.4804713274396395 1.9717041416782162 7 0.3119215486253498 1.493366623999653 8 0.23429890310707077 1.2819859480027227 9 0.17114118357718633 1.053464882917208 >10 0.8677264774199324 10.140494323674458 >50 0.03078230384392087 1.405491455270293 >100 0.011161001833335182 1.1834173305519347 >500 6.377714292553545E-4 0.342309858319866 >1k 4.7832857194151594E-4 0.7745864681892348 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2715 0.29727655259933583 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2253 0.24669026630066432 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2135 0.23376995941052742 No Hit GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 1000 0.10949412618760067 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 932 0.10204852560684381 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.0949412618760067E-4 0.0 8 0.0 0.0 0.0 1.0949412618760067E-4 0.0 9 0.0 0.0 0.0 1.0949412618760067E-4 0.0 10-11 0.0 0.0 0.0 2.1898825237520134E-4 0.0 12-13 0.0 0.0 0.0 3.28482378562802E-4 0.0 14-15 0.0 0.0 0.0 3.28482378562802E-4 1.6424118928140102E-4 16-17 0.0 0.0 0.0 3.28482378562802E-4 3.28482378562802E-4 18-19 0.0 0.0 0.0 3.28482378562802E-4 4.379765047504027E-4 20-21 0.0 0.0 0.0 3.8322944165660236E-4 4.379765047504027E-4 22-23 0.0 0.0 0.0 4.92723567844203E-4 4.379765047504027E-4 24-25 0.0 0.0 0.0 8.21205946407005E-4 4.92723567844203E-4 26-27 0.0 0.0 0.0 0.00164241189281401 5.474706309380033E-4 28-29 0.0 0.0 0.0 0.0036680532272846227 5.474706309380033E-4 30-31 0.0 0.0 0.0 0.012756065700855477 5.474706309380033E-4 32-33 0.0 0.0 0.0 0.0327387437300926 5.474706309380033E-4 34-35 0.0 0.0 0.0 0.05797713981633455 6.56964757125604E-4 36-37 0.0 0.0 0.0 0.09367222495349237 6.56964757125604E-4 38-39 0.0 0.0 0.0 0.15400348848286033 6.56964757125604E-4 40-41 0.0 0.0 0.0 0.22966392967849242 6.56964757125604E-4 42-43 0.0 0.0 0.0 0.29223982279470617 6.56964757125604E-4 44-45 5.4747063093800336E-5 0.0 0.0 0.35848376913820457 6.56964757125604E-4 46-47 1.0949412618760067E-4 0.0 0.0 0.4273008274471116 6.56964757125604E-4 48-49 1.0949412618760067E-4 0.0 0.0 0.5079432513842794 6.56964757125604E-4 50-51 1.0949412618760067E-4 0.0 0.0 0.5941698757570151 6.56964757125604E-4 52-53 1.0949412618760067E-4 0.0 0.0 0.6927145893258556 7.117118202194043E-4 54-55 1.0949412618760067E-4 0.0 0.0 0.7987596505385468 7.664588833132046E-4 56-57 1.0949412618760067E-4 0.0 0.0 0.8985635465585449 7.664588833132046E-4 58-59 1.0949412618760067E-4 0.0 0.0 1.0217991855826893 7.664588833132046E-4 60-61 1.0949412618760067E-4 0.0 0.0 1.1467867306258355 7.664588833132046E-4 62-63 1.0949412618760067E-4 0.0 0.0 1.3052247312192937 7.664588833132046E-4 64-65 1.0949412618760067E-4 0.0 0.0 1.4372199003384463 7.664588833132046E-4 66-67 1.0949412618760067E-4 0.0 0.0 1.583723041177456 8.21205946407005E-4 68-69 1.0949412618760067E-4 0.0 0.0 1.7467597950707934 8.759530095008054E-4 70-71 1.0949412618760067E-4 0.0 0.0 1.9278630797850849 8.759530095008054E-4 72-73 1.0949412618760067E-4 0.0 0.0 2.0901333747951094 0.0010949412618760067 74-75 1.0949412618760067E-4 1.0949412618760067E-4 0.0 2.261655923467986 0.0010949412618760067 76-77 1.0949412618760067E-4 1.0949412618760067E-4 0.0 2.4732533223255238 0.0010949412618760067 78-79 1.0949412618760067E-4 1.0949412618760067E-4 0.0 2.709815381953835 0.0010949412618760067 80-81 1.0949412618760067E-4 1.0949412618760067E-4 0.0 2.9230551927041875 0.0012591824511574076 82-83 1.0949412618760067E-4 1.0949412618760067E-4 0.0 3.1520621576255543 0.0017519060190016107 84-85 1.0949412618760067E-4 1.0949412618760067E-4 0.0 3.3944274059418085 0.0017519060190016107 86-87 1.0949412618760067E-4 1.0949412618760067E-4 0.0 3.6568848264134868 0.0018614001451892114 88-89 1.0949412618760067E-4 1.0949412618760067E-4 0.0 3.951150290542664 0.001970894271376812 90-91 1.0949412618760067E-4 1.0949412618760067E-4 0.0 4.260909173527386 0.001970894271376812 92-93 1.0949412618760067E-4 1.0949412618760067E-4 0.0 4.554627167025625 0.001970894271376812 94-95 1.0949412618760067E-4 1.0949412618760067E-4 0.0 4.8471407251358 0.001970894271376812 96-97 1.0949412618760067E-4 1.0949412618760067E-4 0.0 5.193087416825524 0.001970894271376812 98-99 1.0949412618760067E-4 1.0949412618760067E-4 0.0 5.587813741731825 0.001970894271376812 100-101 1.0949412618760067E-4 1.0949412618760067E-4 0.0 5.996555314790138 0.001970894271376812 102-103 1.0949412618760067E-4 1.0949412618760067E-4 0.0 6.413837429691084 0.0020803883975644127 104-105 1.0949412618760067E-4 1.0949412618760067E-4 0.0 6.858219340823462 0.0021351354606582128 106-107 1.0949412618760067E-4 1.0949412618760067E-4 0.0 7.349026761459381 0.0021898825237520133 108-109 1.0949412618760067E-4 1.0949412618760067E-4 0.0 7.858338689421005 0.0021898825237520133 110-111 1.0949412618760067E-4 1.0949412618760067E-4 0.0 8.414568850454017 0.002299376649939614 112-113 1.0949412618760067E-4 1.0949412618760067E-4 0.0 8.944958397706756 0.0024088707761272146 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1980 0.0 74.872856 1 GTATCAA 3435 0.0 49.249023 1 GTACATG 5825 0.0 40.022717 1 TACATGG 6125 0.0 37.66189 2 ACATGGG 6235 0.0 36.423843 3 CATGGGG 5295 0.0 36.248257 4 TCAACGC 4715 0.0 34.386223 4 ATGGGGG 3460 0.0 34.28264 5 TATCAAC 4900 0.0 33.9395 2 GTGGTAT 850 0.0 33.756718 1 ATCAACG 4830 0.0 33.567505 3 TGGGGGG 2805 0.0 33.560696 6 CAACGCA 4825 0.0 33.355213 5 AACGCAG 4975 0.0 32.69462 6 TGGTATC 890 0.0 30.13837 2 ACGCAGA 5760 0.0 27.51024 7 CGCAGAG 5900 0.0 26.749157 8 GAGTACT 3360 0.0 24.268764 12-13 AGTACTT 3440 0.0 24.136936 12-13 AGAGTAC 6055 0.0 24.047451 10-11 >>END_MODULE