FastQCFastQC Report
Mon 15 Aug 2016
SRR2921316_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921316_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325041
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.059.7296561
GTATCAA23850.055.8905261
TCAACGC30650.043.4873854
CAACGCA31300.042.3925865
ATCAACG33450.039.4914053
AACGCAG34150.039.027466
TATCAAC34350.038.45672
GTACATG67300.036.2532841
TACATGG70400.033.3019262
ACATGGG70400.032.287653
ACGCAGA41650.031.7080127
CATGGGG64300.030.9087354
CGCAGAG43300.030.2249648
ATGGGGG40300.029.0864375
GCAGAGT49350.026.1579449
TGGGGGG25900.025.2700396
GTGTACG3101.2732926E-1124.9551531
AGAGTAC44000.023.6600910-11
GTATAGA4800.023.5554241
GACCGTA1602.6150537E-422.3080867
Produced by FastQC (version 0.11.5)