##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921316_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1325041
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.846391922966912	31.0	31.0	34.0	26.0	34.0
2	29.977955399115952	31.0	31.0	34.0	26.0	34.0
3	30.18036573962617	33.0	31.0	34.0	26.0	34.0
4	33.587230130992175	37.0	35.0	37.0	28.0	37.0
5	33.66632881548571	37.0	35.0	37.0	28.0	37.0
6	33.645853222655	37.0	35.0	37.0	28.0	37.0
7	33.60064179146155	37.0	35.0	37.0	28.0	37.0
8	33.46896963943003	37.0	35.0	37.0	28.0	37.0
9	35.07136307480297	39.0	37.0	39.0	28.0	39.0
10-11	35.09197187105909	39.0	37.0	39.0	27.0	39.0
12-13	35.01250112260677	39.0	37.0	39.0	27.0	39.0
14-15	36.25775315631742	40.0	38.0	41.0	27.0	41.0
16-17	36.24035935491808	40.0	38.0	41.0	27.0	41.0
18-19	36.240411051431614	40.0	38.0	41.0	27.0	41.0
20-21	36.267096640783194	40.0	38.0	41.0	27.0	41.0
22-23	36.22352327211007	40.0	37.5	41.0	27.0	41.0
24-25	36.214449967963255	40.0	37.0	41.0	27.0	41.0
26-27	36.177825818219965	40.0	37.0	41.0	27.0	41.0
28-29	36.11148673890091	40.0	37.0	41.0	27.0	41.0
30-31	36.03178995970691	40.0	37.0	41.0	27.0	41.0
32-33	35.94924685349359	40.0	37.0	41.0	27.0	41.0
34-35	35.83481530005487	40.0	36.5	41.0	26.0	41.0
36-37	35.72420513780328	40.0	36.0	41.0	25.0	41.0
38-39	35.62776434842394	40.0	36.0	41.0	25.0	41.0
40-41	35.52010881172734	40.0	36.0	41.0	24.5	41.0
42-43	35.3298829998468	40.0	35.5	41.0	24.0	41.0
44-45	35.093531822788876	40.0	35.0	41.0	23.0	41.0
46-47	34.87241413661917	40.0	35.0	41.0	22.5	41.0
48-49	34.66396549238854	39.0	35.0	41.0	21.5	41.0
50-51	34.48748831168243	39.0	35.0	41.0	20.0	41.0
52-53	34.294807481428876	39.0	34.0	41.0	19.5	41.0
54-55	34.087875771391225	38.5	34.0	40.5	18.0	41.0
56-57	33.843755778123096	38.0	34.0	40.0	16.0	41.0
58-59	33.57784174225552	38.0	34.0	40.0	13.5	41.0
60-61	33.2982209607099	37.0	33.0	40.0	12.5	41.0
62-63	32.97996439355461	37.0	33.0	40.0	9.0	41.0
64-65	32.63257287887696	36.0	33.0	39.0	8.0	41.0
66-67	32.300927292061154	36.0	32.5	39.0	7.0	40.5
68-69	31.95976426389825	35.0	32.0	38.5	6.5	40.0
70-71	31.91034088756499	35.0	32.0	38.5	2.0	40.0
72-73	31.628597530189634	35.0	32.0	37.0	2.0	39.5
74-75	31.307464448269904	35.0	32.0	37.0	2.0	39.0
76-77	31.01064533097466	35.0	32.0	36.5	2.0	39.0
78-79	30.717412517801336	35.0	32.0	36.0	2.0	37.5
80-81	30.441326721210892	35.0	32.0	35.5	2.0	37.0
82-83	30.218047215142775	35.0	31.5	35.0	2.0	37.0
84-85	29.999958869197254	35.0	31.0	35.0	2.0	36.0
86-87	29.831604078666246	35.0	31.0	35.0	2.0	36.0
88-89	29.6941064465175	35.0	31.0	35.0	2.0	36.0
90-91	29.571654009196696	35.0	31.0	35.0	2.0	35.0
92-93	29.467816090219095	35.0	31.0	35.0	2.0	35.0
94-95	29.349341265666496	35.0	31.0	35.0	2.0	35.0
96-97	29.267609077756838	34.0	31.0	35.0	2.0	35.0
98-99	29.19495396746214	34.0	31.0	35.0	2.0	35.0
100-101	29.110705253648753	34.0	30.5	35.0	2.0	35.0
102-103	28.29352676634157	33.5	29.0	34.5	2.0	35.0
104-105	28.892450875105	34.0	30.0	35.0	2.0	35.0
106-107	28.981579815266095	34.0	30.0	35.0	2.0	35.0
108-109	28.935422752956324	34.0	30.0	35.0	2.0	35.0
110-111	28.861801257470525	34.0	30.0	35.0	2.0	35.0
112-113	28.78708319214273	34.0	30.0	35.0	2.0	35.0
114-115	28.674199137988936	34.0	30.0	35.0	2.0	35.0
116-117	28.557322754541183	34.0	30.0	35.0	2.0	35.0
118-119	28.56225618678969	34.0	29.5	35.0	2.0	35.0
120-121	28.74241664974895	35.0	31.0	35.0	2.0	35.0
122-123	28.62627231912069	35.0	31.0	35.0	2.0	35.0
124-125	28.069665768832813	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60254.0
3	8361.0
4	5145.0
5	4028.0
6	4226.0
7	4867.0
8	5087.0
9	5367.0
10	5822.0
11	6267.0
12	6103.0
13	6482.0
14	6620.0
15	5959.0
16	5149.0
17	4479.0
18	4243.0
19	3762.0
20	3795.0
21	3813.0
22	4373.0
23	5598.0
24	8206.0
25	10431.0
26	11082.0
27	12214.0
28	14579.0
29	17470.0
30	21504.0
31	26514.0
32	33817.0
33	46536.0
34	72009.0
35	119920.0
36	250195.0
37	406318.0
38	104388.0
39	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28473797910355	18.53318720005314	13.629570250404207	24.55250457043911
2	16.08577348222676	19.082335447982278	39.75439377757315	25.077497292217814
3	19.898013481965553	23.710355873470903	28.161321658322517	28.230308986241027
4	13.507420046087992	15.409814040307745	35.10710967296848	35.975656240635786
5	14.612409097942134	37.62981021424016	32.847767592638014	14.910013095179691
6	33.638124687646744	34.519734686574736	16.787204198631763	15.054936427146764
7	29.55417916463504	31.898463410326354	19.692848117794643	18.854509307243966
8	27.060200787541238	35.406824602625285	19.010206939027825	18.52276767080565
9	27.09168428369696	16.626149383863076	18.542223134626315	37.73994319781365
10-11	26.355002428390772	26.004234871993425	26.237903802516545	21.402858897099254
12-13	25.924793825676183	23.26066004463494	26.805190453225013	24.009355676463862
14-15	23.65081467544117	24.673059992430048	23.810100639596733	27.866024692532047
16-17	24.017550301266084	27.7494232981872	24.50879873569	23.724227664856716
18-19	24.058055566457252	27.072232469818232	25.922547797236472	22.947164166488047
20-21	24.93472560224335	25.835793426861542	25.809302530937178	23.42017843995793
22-23	24.872856619399304	25.25569244226613	25.808493030652148	24.062957907682414
24-25	24.37960220426551	25.50290514586485	25.81645787680008	24.30103477306956
26-27	24.13328689278384	25.802656092510784	25.828769467577718	24.23528754712766
28-29	24.15353055984495	26.171570159112555	25.731976627465897	23.9429226535766
30-31	24.718948906079067	25.758133226765807	25.708585365154157	23.81433250200097
32-33	24.431018411946066	25.732383363963308	25.662909306353797	24.17368891773683
34-35	24.36301706395	25.82291349832485	25.887519368553093	23.926550069172055
36-37	24.391577579306905	25.85931612455739	25.817465915098794	23.93164038103691
38-39	24.38961433973742	26.023133502369696	25.68142546028696	23.90582669760593
40-41	24.457567711580293	25.7652695406194	25.774515116705587	24.002647631094725
42-43	24.16886232877798	25.809438919334234	25.683171051827188	24.338527700060606
44-45	24.122371719059323	25.80249410738262	25.910534860218736	24.164599313339316
46-47	24.13325936744067	25.716582322906405	25.81854958922513	24.331608720427795
48-49	24.049312105338434	26.036559156827604	25.647713854538495	24.266414883295464
50-51	24.039380276502705	26.104848533334142	25.72242708935545	24.13334410080771
52-53	24.252047007395568	25.893992602169234	25.643687503915146	24.210272886520055
54-55	23.972631946779394	25.691720337470393	25.882252162398473	24.453395553351744
56-57	24.14751103525129	25.871451822830675	25.580801210000164	24.40023593191787
58-59	24.153816951322092	25.985715088970306	25.727330807731075	24.133137151976523
60-61	24.44557904762767	25.950500609063038	25.80053676038841	23.803383582920876
62-63	24.286338015040986	25.912363172411347	25.739077472519384	24.06222134002828
64-65	24.127945370689726	25.725790809530242	25.757828472841194	24.388435346938834
66-67	24.14096942897585	25.925426972825967	25.63444507211906	24.299158526079125
68-69	24.00792018530245	25.880492298310376	25.814943594906747	24.296643921480428
70-71	23.97374619713824	25.736402339943954	25.97738547263411	24.312465990283695
72-73	23.978683669014224	26.108467701917025	25.59438970832687	24.318458920741875
74-75	23.896632491836794	26.095839209117276	25.771914803051015	24.235613495994908
76-77	23.90288836033164	25.729278813697153	25.738637371362284	24.629195454608922
78-79	23.823583018524033	25.758174381522835	25.899456494052682	24.518786105900453
80-81	23.971148886102327	26.087662012989345	25.568722337462486	24.372466763445846
82-83	24.064015939833165	26.005437807669157	25.82139764110092	24.109148611396755
84-85	24.080718013394634	26.07206323940032	25.520375173546444	24.326843573658604
86-87	23.741306374130637	26.24771220371859	25.640262195605168	24.370719226545607
88-89	23.70627032567112	25.957462224157673	25.76108293892695	24.57518451124426
90-91	23.99854640028408	25.757847344105016	25.839546289716914	24.404059965893993
92-93	24.137582290685895	25.84236038650442	25.736356586368192	24.283700736441496
94-95	23.96968976539003	25.86811727102225	25.672715883061066	24.489477080526655
96-97	23.75185339350738	25.756556267498503	25.9021483703534	24.589441968640713
98-99	23.580116651873855	25.85681314567597	25.767565707247268	24.795504495202902
100-101	23.659472657355586	25.96843642566295	25.856167614504756	24.515923302476704
102-103	23.542650563309326	25.871549246876846	25.917851745211728	24.667948444602096
104-105	23.624506929682788	25.91494190326935	25.853620616329877	24.606930550717987
106-107	23.639646071933573	25.720692383950137	25.982247745872794	24.657413798243493
108-109	23.609427106270235	25.742462167911416	25.90831541778603	24.739795308032317
110-111	23.381409125507137	26.11878201284344	25.985117247245476	24.514691614403944
112-113	23.42510184096771	26.220195361070807	25.913323383516385	24.441379414445098
114-115	23.454662502698078	26.213077860074353	25.887006818020588	24.445252819206985
116-117	23.249673587369152	26.124255665994973	25.935389166874213	24.690681579761662
118-119	23.333044822163103	25.886141901384363	26.048060921464362	24.732752354988175
120-121	23.24067906627444	25.894751143004324	26.21567920779375	24.64889058292749
122-123	23.196973333272958	26.072170877162794	26.044963781733436	24.685892007830816
124-125	23.130689740401614	25.95414057444091	25.992819016963796	24.92235066819368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	292.0
1	235.5
2	166.5
3	139.5
4	115.5
5	95.0
6	109.5
7	136.5
8	168.5
9	227.0
10	262.0
11	291.0
12	317.5
13	341.5
14	381.5
15	417.0
16	472.0
17	535.0
18	558.0
19	607.5
20	701.5
21	789.5
22	889.0
23	1066.0
24	1276.5
25	1640.0
26	2171.0
27	2797.5
28	3632.5
29	4790.5
30	6399.5
31	7938.5
32	9721.0
33	12049.5
34	14459.0
35	16875.0
36	19923.0
37	22984.5
38	25581.5
39	28708.0
40	32088.5
41	34983.0
42	37474.5
43	41211.5
44	45153.0
45	48368.0
46	51429.0
47	54188.0
48	57469.0
49	59566.5
50	60385.0
51	61061.5
52	60491.5
53	59181.5
54	57761.0
55	55778.5
56	52294.0
57	47593.5
58	42743.0
59	37585.0
60	31730.5
61	26204.0
62	21327.0
63	16101.5
64	11520.0
65	8442.0
66	6216.5
67	4575.0
68	3398.0
69	2510.0
70	1837.5
71	1304.5
72	898.0
73	596.5
74	405.5
75	282.0
76	218.5
77	147.0
78	92.5
79	68.5
80	49.0
81	33.5
82	23.5
83	21.0
84	19.5
85	17.0
86	9.0
87	7.5
88	11.5
89	9.5
90	9.5
91	6.5
92	2.5
93	2.5
94	4.5
95	4.5
96	3.0
97	2.5
98	1.5
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016829667912162716
2	0.011773220602230421
3	0.012075098053569664
4	0.013886362761605112
5	0.00950913971718611
6	0.03162166302778555
7	0.011999628690734853
8	0.011924159327900042
9	0.006943181380802556
10-11	0.006754507973715531
12-13	0.0052828553984367276
14-15	0.004943243265680081
16-17	0.0037357334603231145
18-19	0.005207386035601917
20-21	0.004339488363001597
22-23	0.0045658964515060295
24-25	0.0048677739028452705
26-27	0.0037357334603231145
28-29	0.005056447309932296
30-31	0.004490427088671219
32-33	0.006490365203793693
34-35	0.006339426478124073
36-37	0.005848875619697805
38-39	0.0059998143453674265
40-41	0.006263957115289262
42-43	0.006037549026784832
44-45	0.0058866103011152105
46-47	0.007999752460489902
48-49	0.006980916062219962
50-51	0.004754569858593055
52-53	0.004377223044419003
54-55	0.004943243265680081
56-57	0.006226222433871858
58-59	0.005773406256862995
60-61	0.0041508149559145715
62-63	0.004641365814340839
64-65	0.002867835787722795
66-67	0.0033583866461490623
68-69	0.005433794124106348
70-71	0.00392440686741014
72-73	0.0035847947346534935
74-75	0.003056509194809821
76-77	0.0038112028231579247
78-79	0.002452754292131338
80-81	0.0023772849292965277
82-83	0.004414957725836408
84-85	0.008112956504742117
86-87	0.00573567157544559
88-89	0.004075345593079762
90-91	0.004113080274497166
92-93	0.00667903861088072
94-95	0.00573567157544559
96-97	0.008037487141907308
98-99	0.005509263486941159
100-101	0.007245058832141798
102-103	0.004414957725836408
104-105	0.003962141548827546
106-107	0.006226222433871858
108-109	0.005622467531193374
110-111	0.006377161159541478
112-113	0.004414957725836408
114-115	0.001735795345200639
116-117	0.0023395502478791223
118-119	0.16282515031610345
120-121	0.010075159938447188
122-123	0.0014339178938613975
124-125	0.0010188363982699404
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1325041.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.27558399805513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.38446823837262	54.60078744867369
2	9.71911099922945	12.882795148317058
3	3.118771433931542	6.200951944207944
4	1.5235467097307462	4.0389579174288235
5	0.8621414022205118	2.856946246053329
6	0.5323330836306781	2.116841159946523
7	0.37265060411620526	1.7288345490520687
8	0.26796088709672555	1.4207411424777923
9	0.20283459180963048	1.2098682924471351
>10	0.9798068114820124	10.900741126749145
>50	0.030224675430041623	1.2912184216308207
>100	0.005808875973015068	0.6054795576848696
>500	3.416869768210835E-4	0.1458370453307562
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	7.546936283481039E-5
10-11	0.0	0.0	0.0	0.0	7.546936283481039E-5
12-13	0.0	0.0	0.0	0.0	1.1320404425221559E-4
14-15	0.0	0.0	0.0	0.0	1.8867340708702597E-4
16-17	0.0	0.0	0.0	0.0	3.0187745133924157E-4
18-19	0.0	7.546936283481039E-5	0.0	0.0	3.0187745133924157E-4
20-21	0.0	7.546936283481039E-5	0.0	0.0	4.150814955914572E-4
22-23	0.0	7.546936283481039E-5	0.0	0.0	4.905508584262675E-4
24-25	0.0	7.546936283481039E-5	0.0	0.0	5.282855398436728E-4
26-27	0.0	7.546936283481039E-5	0.0	3.7734681417405196E-5	5.282855398436728E-4
28-29	0.0	7.546936283481039E-5	0.0	6.037549026784831E-4	5.282855398436728E-4
30-31	0.0	7.546936283481039E-5	0.0	0.003207447920479442	5.282855398436728E-4
32-33	0.0	7.546936283481039E-5	0.0	0.009924221212777567	6.037549026784831E-4
34-35	0.0	7.546936283481039E-5	0.0	0.018980544752954815	6.792242655132935E-4
36-37	0.0	1.1320404425221559E-4	0.0	0.03279143815172512	6.792242655132935E-4
38-39	0.0	1.5093872566962079E-4	0.0	0.05863969492264768	6.792242655132935E-4
40-41	0.0	1.5093872566962079E-4	0.0	0.08943119495925031	7.54693628348104E-4
42-43	0.0	1.5093872566962079E-4	0.0	0.11561906386292953	7.924283097655092E-4
44-45	0.0	1.5093872566962079E-4	0.0	0.1446370338729141	8.301629911829143E-4
46-47	0.0	1.5093872566962079E-4	0.0	0.17901332864417024	8.301629911829143E-4
48-49	0.0	1.5093872566962079E-4	0.0	0.21648386729165361	8.301629911829143E-4
50-51	0.0	1.5093872566962079E-4	0.0	0.2585957717534778	8.301629911829143E-4
52-53	0.0	1.5093872566962079E-4	0.0	0.30633014374649536	9.4336703543513E-4
54-55	0.0	1.5093872566962079E-4	0.0	0.35832853473967974	0.0010565710796873456
56-57	0.0	1.5093872566962079E-4	0.0	0.41304382279491725	0.0010943057611047507
58-59	0.0	1.5093872566962079E-4	0.0	0.47828708696561084	0.0011320404425221558
60-61	0.0	1.5093872566962079E-4	0.0	0.5465868603311143	0.0011320404425221558
62-63	0.0	1.5093872566962079E-4	0.0	0.6191883873782018	0.0012829791681917768
64-65	0.0	1.5093872566962079E-4	0.0	0.6902805271685932	0.0012829791681917768
66-67	0.0	2.2640808850443118E-4	0.0	0.7757118458975987	0.0012829791681917768
68-69	0.0	2.2640808850443118E-4	0.0	0.8731050586359215	0.0012829791681917768
70-71	0.0	2.2640808850443118E-4	0.0	0.9767622284895336	0.0013207138496091819
72-73	0.0	2.2640808850443118E-4	0.0	1.078947745767867	0.0015848566195310182
74-75	0.0	2.2640808850443118E-4	0.0	1.1854727514092018	0.0015848566195310182
76-77	0.0	2.2640808850443118E-4	0.0	1.3069784255732464	0.0015848566195310182
78-79	0.0	2.2640808850443118E-4	0.0	1.440030912251017	0.0015848566195310182
80-81	0.0	2.2640808850443118E-4	0.0	1.5863660067877143	0.001735795345200639
82-83	0.0	2.2640808850443118E-4	0.0	1.741568751457502	0.002113142159374691
84-85	0.0	2.2640808850443118E-4	0.0	1.9033750653753354	0.002113142159374691
86-87	0.0	2.2640808850443118E-4	0.0	2.083180822329271	0.0021886115222095014
88-89	0.0	2.2640808850443118E-4	0.0	2.290268753947991	0.0022640808850443116
90-91	0.0	2.2640808850443118E-4	0.0	2.5113185176911506	0.0022640808850443116
92-93	0.0	2.2640808850443118E-4	0.0	2.7262552630446906	0.002490488973548743
94-95	0.0	2.2640808850443118E-4	0.0	2.9537199226288093	0.0025659583363835535
96-97	0.0	2.2640808850443118E-4	0.0	3.2051461049129797	0.0025659583363835535
98-99	0.0	2.2640808850443118E-4	0.0	3.484873298260205	0.0025659583363835535
100-101	0.0	2.2640808850443118E-4	0.0	3.7842225259444806	0.0025659583363835535
102-103	0.0	2.2640808850443118E-4	0.0	4.104665440541085	0.0025659583363835535
104-105	0.0	2.2640808850443118E-4	0.0	4.4372211878726775	0.0026036930178009584
106-107	0.0	2.2640808850443118E-4	0.0	4.792681886824634	0.002679162380635769
108-109	0.0	2.2640808850443118E-4	0.0	5.17855673899902	0.002716897062053174
110-111	0.0	2.2640808850443118E-4	0.0	5.59722302932513	0.0027546317434705794
112-113	0.0	2.2640808850443118E-4	0.0	6.020342012058494	0.0027923664248879847
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1345	0.0	59.729656	1
GTATCAA	2385	0.0	55.890526	1
TCAACGC	3065	0.0	43.487385	4
CAACGCA	3130	0.0	42.392586	5
ATCAACG	3345	0.0	39.491405	3
AACGCAG	3415	0.0	39.02746	6
TATCAAC	3435	0.0	38.4567	2
GTACATG	6730	0.0	36.253284	1
TACATGG	7040	0.0	33.301926	2
ACATGGG	7040	0.0	32.28765	3
ACGCAGA	4165	0.0	31.708012	7
CATGGGG	6430	0.0	30.908735	4
CGCAGAG	4330	0.0	30.224964	8
ATGGGGG	4030	0.0	29.086437	5
GCAGAGT	4935	0.0	26.157944	9
TGGGGGG	2590	0.0	25.270039	6
GTGTACG	310	1.2732926E-11	24.955153	1
AGAGTAC	4400	0.0	23.66009	10-11
GTATAGA	480	0.0	23.555424	1
GACCGTA	160	2.6150537E-4	22.308086	7
>>END_MODULE