FastQCFastQC Report
Mon 15 Aug 2016
SRR2921316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325041
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.1720701472633677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.11856236901348713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.10784571949094404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.067.342491
GTATCAA32350.050.2583581
GTGGTAT7650.042.193551
TCAACGC44450.036.0725984
TATCAAC46400.035.0704652
TGGTATC8600.034.65522
ATCAACG46550.034.189163
CAACGCA46450.033.877795
GTACATG66550.032.6939241
AACGCAG48700.032.417836
TACATGG68600.031.8019562
ACATGGG69750.031.1067073
CATGGGG62400.030.4721414
ACGCAGA57450.026.9517067
ATGGGGG39350.025.9028915
CGCAGAG60750.025.2896948
TATACCG1701.3796416E-524.5440375
AGAGTAC60950.022.4706710-11
ATACCGT1602.5892686E-422.340786
GCAGAGT65550.021.8934639
Produced by FastQC (version 0.11.5)