##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921316_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1325041
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.974308719503775	31.0	30.0	34.0	16.0	34.0
2	29.797331554268887	33.0	31.0	34.0	25.0	34.0
3	30.031662416483716	33.0	31.0	34.0	26.0	34.0
4	32.07414789429157	37.0	33.0	37.0	19.0	37.0
5	31.733683712428522	37.0	33.0	37.0	19.0	37.0
6	31.9001208264499	37.0	33.0	37.0	17.0	37.0
7	32.136028998347975	37.0	35.0	37.0	17.0	37.0
8	32.40341015862905	37.0	35.0	37.0	17.0	37.0
9	34.123144868724815	39.0	37.0	39.0	17.0	39.0
10-11	34.33652241704219	39.0	37.0	39.0	17.5	39.0
12-13	34.38969586601471	39.0	37.0	39.0	19.0	39.0
14-15	35.7823380559545	40.5	38.0	41.0	22.0	41.0
16-17	35.81486648337674	41.0	38.0	41.0	23.0	41.0
18-19	35.8395204374808	41.0	38.0	41.0	23.0	41.0
20-21	35.83380136916518	41.0	38.0	41.0	23.0	41.0
22-23	35.79424410263532	41.0	38.0	41.0	23.0	41.0
24-25	35.74202232232814	41.0	38.0	41.0	22.0	41.0
26-27	35.655760085914324	40.5	37.5	41.0	20.5	41.0
28-29	35.58107862322751	40.0	37.0	41.0	19.0	41.0
30-31	35.47494794500699	40.0	37.0	41.0	18.5	41.0
32-33	35.34335956396821	40.0	36.5	41.0	17.0	41.0
34-35	35.260629293735064	40.0	36.0	41.0	16.0	41.0
36-37	35.181232127911514	40.0	36.0	41.0	15.5	41.0
38-39	35.085190194114745	40.0	36.0	41.0	14.5	41.0
40-41	34.956553042509626	40.0	35.5	41.0	9.0	41.0
42-43	34.80897232613934	40.0	35.0	41.0	8.5	41.0
44-45	34.64730336646187	40.0	35.0	41.0	4.5	41.0
46-47	34.41196649764045	40.0	35.0	41.0	2.0	41.0
48-49	34.18357318754665	40.0	35.0	41.0	2.0	41.0
50-51	33.993898302014806	40.0	35.0	41.0	2.0	41.0
52-53	33.813880476151304	39.0	35.0	41.0	2.0	41.0
54-55	33.62229659308656	39.0	35.0	41.0	2.0	41.0
56-57	33.38691180121974	39.0	34.0	41.0	2.0	41.0
58-59	33.32660347868481	39.0	34.0	41.0	2.0	41.0
60-61	33.08730786443589	38.0	34.0	41.0	2.0	41.0
62-63	32.80706257391281	37.0	34.0	40.5	2.0	41.0
64-65	32.50672960308398	37.0	34.0	40.0	2.0	41.0
66-67	32.1973942693094	36.0	34.0	39.0	2.0	41.0
68-69	31.86604339035547	36.0	33.5	39.0	2.0	41.0
70-71	31.532727289193318	35.0	33.0	39.0	2.0	41.0
72-73	31.195071322321347	35.0	33.0	37.0	2.0	39.5
74-75	30.86132353640378	35.0	33.0	37.0	2.0	39.0
76-77	30.560924907229285	35.0	33.0	36.5	2.0	39.0
78-79	30.303856635379585	35.0	32.0	36.0	2.0	38.0
80-81	30.07462863413283	35.0	32.0	36.0	2.0	37.0
82-83	29.871294171274698	35.0	32.0	35.0	2.0	37.0
84-85	29.69500641866931	35.0	32.0	35.0	2.0	36.0
86-87	29.55158029072308	35.0	32.0	35.0	2.0	36.0
88-89	29.42433630355589	35.0	32.0	35.0	2.0	36.0
90-91	29.320208582225	35.0	32.0	35.0	2.0	35.5
92-93	29.251763530336042	35.0	32.0	35.0	2.0	35.0
94-95	29.18265283866688	35.0	31.0	35.0	2.0	35.0
96-97	29.12042570758188	35.0	31.0	35.0	2.0	35.0
98-99	29.064327066105882	35.0	31.0	35.0	2.0	35.0
100-101	29.014733129012612	35.0	31.0	35.0	2.0	35.0
102-103	28.365360392621813	34.0	29.5	35.0	2.0	35.0
104-105	28.894367419574188	35.0	31.0	35.0	2.0	35.0
106-107	28.953419554564725	35.0	31.0	35.0	2.0	35.0
108-109	29.0241384983559	35.0	31.5	35.0	2.0	35.0
110-111	29.12754963808667	35.0	32.0	35.0	2.0	35.0
112-113	29.095867599568617	35.0	32.0	35.0	2.0	35.0
114-115	29.043243190210717	35.0	32.0	35.0	2.0	35.0
116-117	28.989663716066143	35.0	32.0	35.0	2.0	35.0
118-119	28.92533514057301	35.0	32.0	35.0	2.0	35.0
120-121	28.843248246658028	35.0	32.0	35.0	2.0	35.0
122-123	28.741454792719622	35.0	32.0	35.0	2.0	35.0
124-125	28.242316652843193	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96750.0
3	5252.0
4	4109.0
5	3950.0
6	4159.0
7	4365.0
8	4477.0
9	4451.0
10	4653.0
11	4837.0
12	5497.0
13	6744.0
14	8669.0
15	7332.0
16	4575.0
17	3934.0
18	3519.0
19	3157.0
20	2935.0
21	3027.0
22	3452.0
23	4166.0
24	5629.0
25	7426.0
26	8667.0
27	9942.0
28	11752.0
29	14004.0
30	16616.0
31	20552.0
32	26333.0
33	35316.0
34	53327.0
35	90842.0
36	205353.0
37	455018.0
38	169978.0
39	276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.212543329705646	18.684200760527688	13.201085429480027	25.90217048028664
2	15.76147455059881	19.52173555384324	38.645068341281515	26.071721554276433
3	19.17465195416595	23.306297691920477	28.620623814659318	28.898426539254256
4	14.066899255584625	16.737570557926567	31.504878479587077	37.690651706901726
5	14.621514546664246	37.43974172866388	32.26138631827254	15.677357406399322
6	33.34974331714735	35.06984688125993	16.20767790926739	15.372731892325337
7	29.776901777599292	32.0111299999698	18.703970988323466	19.507997234107442
8	27.41053766086541	36.1763487183977	17.48022367754688	18.93288994319001
9	27.253618605268997	17.15760046733557	18.106385259004437	37.482395668391
10-11	26.936432369771186	26.356853587962085	24.847020484311862	21.85969355795486
12-13	26.560923110961266	23.493652706005303	26.012333266254096	23.93309091677934
14-15	24.287059796640257	24.64199221005237	23.554856038416926	27.516091954890452
16-17	24.01110607143477	27.839591378681867	24.175553813051824	23.973748736831542
18-19	24.066576053118357	26.615025497324236	26.182208701466596	23.136189748090814
20-21	24.933605828046076	25.32483900498173	26.324543919773046	23.41701124719914
22-23	24.803496646518862	24.756781110924113	26.251980127407375	24.187742115149643
24-25	24.336605433341308	25.296537993918676	26.15213416037692	24.214722412363088
26-27	23.949938152857158	25.490154644271385	26.34473197433136	24.215175228540097
28-29	24.039369347816407	25.661281424499315	26.295790092532982	24.00355913515129
30-31	24.596106837448804	25.418685157666822	26.20383067391877	23.781377330965608
32-33	24.222910838230664	25.597736220992406	26.13956851146493	24.039784429312
34-35	24.328643415562233	25.661319159180735	26.34427915815435	23.665758267102678
36-37	24.437923052947042	25.51698400275916	26.325072205312892	23.720020738980907
38-39	24.237400955894948	25.770561061884123	26.274847344346323	23.7171906378746
40-41	24.22894838725745	25.64984781602984	26.22156597418495	23.899637822527755
42-43	24.114197221067123	25.666715218623427	26.076061042639438	24.14302651767002
44-45	24.01808698749699	25.688639068526935	26.283865933205085	24.00940801077099
46-47	24.104423938580013	25.707581878598475	25.828219655089917	24.359774527731595
48-49	24.105480509659703	26.04708080731087	25.507852209856146	24.339586473173284
50-51	24.052312343542578	26.212622854689027	25.611848991842518	24.12321580992588
52-53	24.250155278214034	26.014855389380404	25.519210348962783	24.215778983442775
54-55	23.897449211005547	25.865841132463068	25.784636098052815	24.452073558478567
56-57	23.995710321416468	26.002138801742742	25.536568302414793	24.465582574425998
58-59	24.086613168950997	25.98055456397198	25.689242823429616	24.243589443647405
60-61	24.30807801418975	25.794862196716934	25.936291782669368	23.96076800642395
62-63	24.153818636555396	25.921197910102407	25.86821841739237	24.05676503594983
64-65	24.0278602699841	25.621320396878282	25.844671976187904	24.506147356949707
66-67	23.96820173866318	25.91965078816429	25.771504428919556	24.34064304425297
68-69	23.8719783010488	25.899915549782985	25.83769106012569	24.390415089042527
70-71	23.92808977231648	25.820295371992263	25.863992133073616	24.38762272261764
72-73	23.87292166808423	26.07440071665707	25.570755923778965	24.481921691479734
74-75	23.828206070604608	26.149907814173297	25.675130052579505	24.34675606264259
76-77	23.871563219553206	25.702940512784135	25.615546990621425	24.809949277041238
78-79	23.770207865266055	25.821804759248955	25.75169372117542	24.656293654309565
80-81	23.91265628761676	26.082023122303383	25.44664655659712	24.55867403348274
82-83	23.931787771095387	25.991007070724603	25.668375544605787	24.40882961357422
84-85	23.93039158788294	26.023723039513495	25.43585443771174	24.610030934891828
86-87	23.614740977826347	26.26790416296552	25.50864463816591	24.60871022104222
88-89	23.562742586833163	26.034137811584696	25.550530134539233	24.852589467042908
90-91	23.811272183755893	25.758684357195122	25.70045972179718	24.729583737251804
92-93	23.83297686556914	25.966085500944125	25.588624628123874	24.612313005362864
94-95	23.78586775805428	26.017723225168126	25.403817693188362	24.79259132358923
96-97	23.59397375545975	25.88304104600813	25.53173023404998	24.99125496448214
98-99	23.443614197598414	25.919688522845707	25.430231970180543	25.206465309375332
100-101	23.534743453221445	26.005761331158812	25.55385078650396	24.905644429115778
102-103	23.364899652161707	25.93055611109392	25.586679959337104	25.117864277407264
104-105	23.453462949448355	25.994403192052168	25.488758461058943	25.06337539744053
106-107	23.484489719370842	25.798381257025728	25.56721851143418	25.14991051216925
108-109	23.442053526786978	25.77991188928615	25.566106619623973	25.211927964302898
110-111	23.240494521479942	26.162900172334613	25.525975282982344	25.0706300232031
112-113	23.19299851664128	26.234091439952273	25.532637476712853	25.040272566693595
114-115	23.228799421602588	26.177614948373275	25.44839195481195	25.14519367521219
116-117	22.96612206531669	26.12795949394602	25.538579503281607	25.367338937455685
118-119	23.12711314375415	25.912198877015037	25.5953405180221	25.36534746120872
120-121	23.08073718529252	25.895406931111513	25.683715208597473	25.34014067499849
122-123	22.97338602192574	26.11768292959039	25.483767012976426	25.425164035507443
124-125	22.841066804725287	26.05455227423151	25.387931392311636	25.716449528731566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	33.5
2	42.0
3	41.0
4	40.5
5	62.5
6	128.5
7	330.0
8	502.0
9	630.0
10	661.0
11	585.0
12	606.0
13	689.5
14	786.5
15	850.5
16	929.5
17	1020.5
18	1099.5
19	1174.0
20	1237.5
21	1312.0
22	1434.0
23	1613.0
24	1773.0
25	2060.0
26	2517.0
27	3103.5
28	3722.0
29	4591.5
30	5980.0
31	7335.0
32	9034.5
33	11294.0
34	13663.5
35	15966.5
36	18515.0
37	21445.0
38	24155.5
39	27363.5
40	30681.5
41	33510.5
42	36306.5
43	39527.5
44	43333.0
45	47103.0
46	50358.0
47	53405.5
48	56872.0
49	59730.5
50	61000.0
51	61754.5
52	61316.5
53	59933.5
54	58732.5
55	56598.5
56	53237.5
57	48479.0
58	43564.0
59	38266.0
60	32219.0
61	26624.0
62	21542.0
63	16380.0
64	11679.0
65	8519.5
66	6264.5
67	4682.5
68	3589.0
69	2639.5
70	1935.0
71	1420.0
72	1044.0
73	784.0
74	581.5
75	433.0
76	338.5
77	256.0
78	204.5
79	168.5
80	157.0
81	148.5
82	126.0
83	121.0
84	115.5
85	102.0
86	94.0
87	91.0
88	83.5
89	82.5
90	81.5
91	73.5
92	68.0
93	68.5
94	71.5
95	61.5
96	48.0
97	42.0
98	31.0
99	13.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35032878227918984
2	0.0
3	0.0
4	0.018339055168858927
5	0.18120194016637975
6	0.0486022696656179
7	0.02513129782399186
8	0.007395997557811418
9	0.006263957115289262
10-11	0.002037672796539881
12-13	0.05558318572783785
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.7734681417405196E-5
92-93	2.2640808850443118E-4
94-95	0.0
96-97	2.641427699218364E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	3.7734681417405196E-5
108-109	2.641427699218364E-4
110-111	1.88673407087026E-4
112-113	5.66020221261078E-4
114-115	3.7734681417405196E-5
116-117	3.3961213275664675E-4
118-119	7.546936283481039E-5
120-121	7.546936283481039E-5
122-123	0.001735795345200639
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1325041.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.18663356677155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.68246487870469	56.22343103769762
2	8.929009673141454	11.99820202047027
3	2.908350920353723	5.862069227081651
4	1.4033134637816567	3.7713562988166043
5	0.796517470291279	2.6757663702995997
6	0.4925160530460385	1.9854297349054084
7	0.3650848257055076	1.7170174283825217
8	0.2591151300611186	1.3927258636018178
9	0.18923451046583736	1.144262674157005
>10	0.9379900555492288	10.722980222620974
>50	0.028653219400537774	1.2782678865424275
>100	0.007188235689310107	0.7136333607766987
>500	2.2462552385771098E-4	0.11643348975244297
>1k	3.3693828578656645E-4	0.39842438489494414
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2280	0.1720701472633677	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1571	0.11856236901348713	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1429	0.10784571949094404	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.546936283481039E-5
7	0.0	0.0	0.0	0.0	7.546936283481039E-5
8	0.0	0.0	0.0	0.0	7.546936283481039E-5
9	0.0	0.0	0.0	0.0	7.546936283481039E-5
10-11	0.0	0.0	0.0	0.0	7.546936283481039E-5
12-13	0.0	0.0	0.0	0.0	7.546936283481039E-5
14-15	0.0	0.0	0.0	0.0	7.546936283481039E-5
16-17	0.0	0.0	0.0	0.0	7.546936283481039E-5
18-19	0.0	0.0	0.0	0.0	1.5093872566962079E-4
20-21	0.0	0.0	0.0	0.0	2.2640808850443118E-4
22-23	0.0	0.0	0.0	0.0	3.396121327566468E-4
24-25	0.0	0.0	0.0	7.546936283481039E-5	3.77346814174052E-4
26-27	0.0	0.0	0.0	1.5093872566962079E-4	4.5281617700886236E-4
28-29	0.0	0.0	0.0	7.924283097655091E-4	4.5281617700886236E-4
30-31	0.0	0.0	0.0	0.0036225294160708993	4.5281617700886236E-4
32-33	0.0	0.0	0.0	0.01067891484112567	5.282855398436728E-4
34-35	0.0	0.0	0.0	0.019961646469807352	6.037549026784831E-4
36-37	0.0	0.0	0.0	0.033697070505742846	6.037549026784831E-4
38-39	0.0	0.0	0.0	0.0587906336483173	6.037549026784831E-4
40-41	0.0	0.0	0.0	0.08829915451672815	7.54693628348104E-4
42-43	0.0	0.0	0.0	0.11267575871237193	7.54693628348104E-4
44-45	0.0	0.0	0.0	0.14003340273999068	7.924283097655092E-4
46-47	0.0	0.0	0.0	0.17301351429880282	9.4336703543513E-4
48-49	0.0	0.0	0.0	0.2083331761054941	9.81101716852535E-4
50-51	0.0	0.0	0.0	0.24806779563802178	9.81101716852535E-4
52-53	0.0	0.0	0.0	0.2927456584362295	0.0011320404425221558
54-55	0.0	0.0	0.0	0.3419139483231085	0.001169775123939561
56-57	0.0	0.0	0.0	0.3950066450773976	0.0012075098053569663
58-59	0.0	0.0	0.0	0.457344338778951	0.0012075098053569663
60-61	0.0	0.0	0.0	0.5213800931442876	0.0012075098053569663
62-63	0.0	0.0	0.0	0.5902836214124696	0.0012829791681917768
64-65	0.0	0.0	0.0	0.6582815173266336	0.0012829791681917768
66-67	7.546936283481039E-5	0.0	0.0	0.7388073274713763	0.0013207138496091819
68-69	7.546936283481039E-5	0.0	0.0	0.8316723784396105	0.001358448531026587
70-71	7.546936283481039E-5	0.0	0.0	0.9294806726735247	0.0013961832124439921
72-73	7.546936283481039E-5	0.0	0.0	1.0254022328365688	0.001547121938113613
74-75	7.546936283481039E-5	0.0	0.0	1.124946322415684	0.0015848566195310182
76-77	7.546936283481039E-5	0.0	0.0	1.2389805296590821	0.0015848566195310182
78-79	7.546936283481039E-5	0.0	0.0	1.3634295089736845	0.0015848566195310182
80-81	7.546936283481039E-5	0.0	0.0	1.5004064025188655	0.0016603259823658287
82-83	1.1320404425221559E-4	0.0	0.0	1.64693017046265	0.0018112647080354494
84-85	1.5093872566962079E-4	0.0	0.0	1.7984349163535316	0.0018112647080354494
86-87	1.5093872566962079E-4	0.0	0.0	1.9673353503778372	0.0018112647080354494
88-89	1.5093872566962079E-4	0.0	0.0	2.1623859186244045	0.0018112647080354494
90-91	1.5093872566962079E-4	0.0	0.0	2.3711719109069076	0.0018112647080354494
92-93	1.5093872566962079E-4	7.546936283481039E-5	0.0	2.5734298033041996	0.0018112647080354494
94-95	1.5093872566962079E-4	7.546936283481039E-5	0.0	2.7878005284364784	0.00188673407087026
96-97	1.5093872566962079E-4	7.546936283481039E-5	0.0	3.026887469897158	0.00188673407087026
98-99	1.5093872566962079E-4	7.546936283481039E-5	0.0	3.290917035774742	0.00188673407087026
100-101	1.5093872566962079E-4	7.546936283481039E-5	0.0	3.5728328406441765	0.00188673407087026
102-103	1.5093872566962079E-4	7.546936283481039E-5	0.0	3.8758800671073574	0.002113142159374691
104-105	1.5093872566962079E-4	7.546936283481039E-5	0.0	4.189908085863003	0.0021886115222095014
106-107	1.5093872566962079E-4	1.5093872566962079E-4	0.0	4.528614586265633	0.0022263462036269067
108-109	1.5093872566962079E-4	1.5093872566962079E-4	0.0	4.895207016235724	0.0022640808850443116
110-111	1.5093872566962079E-4	1.5093872566962079E-4	0.0	5.291232497711391	0.0023395502478791223
112-113	1.5093872566962079E-4	1.5093872566962079E-4	0.0	5.694012487160775	0.0023395502478791223
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1935	0.0	67.34249	1
GTATCAA	3235	0.0	50.258358	1
GTGGTAT	765	0.0	42.19355	1
TCAACGC	4445	0.0	36.072598	4
TATCAAC	4640	0.0	35.070465	2
TGGTATC	860	0.0	34.6552	2
ATCAACG	4655	0.0	34.18916	3
CAACGCA	4645	0.0	33.87779	5
GTACATG	6655	0.0	32.693924	1
AACGCAG	4870	0.0	32.41783	6
TACATGG	6860	0.0	31.801956	2
ACATGGG	6975	0.0	31.106707	3
CATGGGG	6240	0.0	30.472141	4
ACGCAGA	5745	0.0	26.951706	7
ATGGGGG	3935	0.0	25.902891	5
CGCAGAG	6075	0.0	25.289694	8
TATACCG	170	1.3796416E-5	24.544037	5
AGAGTAC	6095	0.0	22.47067	10-11
ATACCGT	160	2.5892686E-4	22.34078	6
GCAGAGT	6555	0.0	21.893463	9
>>END_MODULE