FastQCFastQC Report
Mon 15 Aug 2016
SRR2921315_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921315_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320187
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.055.6038861
GTATCAA24950.047.4725841
GTACATG74850.039.361691
TACATGG77850.037.8391272
TCAACGC31500.037.406684
ACATGGG80900.036.2654383
CAACGCA32750.035.9789475
ATCAACG33500.035.5287323
CATGGGG68150.034.754564
AACGCAG34950.033.881886
TATCAAC34650.033.8343282
ATGGGGG41450.033.0215765
GTGGTAT9200.027.1720141
ACGCAGA44050.026.741287
TGGGGGG30350.026.4689486
CGCAGAG45800.025.8494018
GCAGAGT51350.022.8229759
AGAGTAC45450.022.44710210-11
TGGTATC9750.021.9731542
GAGTACA52200.021.8922841
Produced by FastQC (version 0.11.5)