##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921315_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1320187
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.578229447797927	31.0	30.0	34.0	25.0	34.0
2	29.532427603059265	31.0	31.0	34.0	24.0	34.0
3	29.66456948901936	31.0	31.0	34.0	24.0	34.0
4	33.09828153132852	37.0	35.0	37.0	26.0	37.0
5	33.07043774859168	37.0	35.0	37.0	26.0	37.0
6	33.26635014585055	37.0	35.0	37.0	27.0	37.0
7	33.058332645299494	37.0	35.0	37.0	26.0	37.0
8	33.09733166589279	37.0	35.0	37.0	26.0	37.0
9	34.665911723111954	39.0	37.0	39.0	26.0	39.0
10-11	34.670863673100854	39.0	36.0	39.0	26.0	39.0
12-13	34.60018239840265	39.0	35.5	39.0	25.5	39.0
14-15	35.7954187550703	40.0	37.0	41.0	25.0	41.0
16-17	35.76220717216576	40.0	37.0	41.0	25.0	41.0
18-19	35.731583480219086	40.0	37.0	41.0	25.0	41.0
20-21	35.79022441517755	40.0	37.0	41.0	25.0	41.0
22-23	35.727491635654644	40.0	37.0	41.0	25.0	41.0
24-25	35.726651603144106	40.0	37.0	41.0	25.0	41.0
26-27	35.71031793223233	40.0	37.0	41.0	25.0	41.0
28-29	35.6497969605821	40.0	36.0	41.0	25.0	41.0
30-31	35.57052637240027	40.0	36.0	41.0	25.0	41.0
32-33	35.49004383469918	40.0	36.0	41.0	24.0	41.0
34-35	35.3556640082049	40.0	36.0	41.0	24.0	41.0
36-37	35.23182359771759	40.0	35.5	41.0	23.0	41.0
38-39	35.12003602519946	40.0	35.0	41.0	22.5	41.0
40-41	35.013521569292834	40.0	35.0	41.0	21.5	41.0
42-43	34.776367287361566	40.0	35.0	41.0	19.0	41.0
44-45	34.45078765356726	39.5	35.0	41.0	17.0	41.0
46-47	34.18893649157279	39.0	34.0	41.0	15.0	41.0
48-49	33.958578216570835	39.0	34.0	41.0	11.0	41.0
50-51	33.77291474616854	39.0	34.0	40.0	9.0	41.0
52-53	33.57578964192194	38.0	33.5	40.0	8.0	41.0
54-55	33.37419623129148	38.0	33.0	40.0	7.0	41.0
56-57	33.13328452711623	38.0	33.0	40.0	3.5	41.0
58-59	32.86045954095897	37.5	33.0	40.0	2.0	41.0
60-61	32.58076961824348	37.0	32.0	40.0	2.0	41.0
62-63	32.266025191885696	36.5	32.0	39.5	2.0	41.0
64-65	31.927046319953156	36.0	31.5	39.0	2.0	41.0
66-67	31.594388143497852	35.5	31.0	39.0	2.0	40.0
68-69	31.250033896713116	35.0	31.0	38.5	2.0	40.0
70-71	31.237742834916567	35.0	31.0	38.0	2.0	40.0
72-73	30.968656334292035	35.0	31.0	37.0	2.0	39.5
74-75	30.644453020670554	35.0	31.0	37.0	2.0	39.0
76-77	30.34355436010202	35.0	31.0	36.0	2.0	39.0
78-79	30.05920487021914	35.0	31.0	36.0	2.0	37.5
80-81	29.771414201169986	35.0	31.0	35.5	2.0	37.0
82-83	29.535744557399823	35.0	30.0	35.0	2.0	37.0
84-85	29.31571890951812	35.0	30.0	35.0	2.0	36.0
86-87	29.137851683132766	34.0	30.0	35.0	2.0	36.0
88-89	28.98808085521218	34.0	30.0	35.0	2.0	36.0
90-91	28.857649333011157	34.0	30.0	35.0	2.0	35.0
92-93	28.73884267910531	34.0	30.0	35.0	2.0	35.0
94-95	28.61552795172199	34.0	29.0	35.0	2.0	35.0
96-97	28.519311279386933	34.0	29.0	35.0	2.0	35.0
98-99	28.435023598929547	34.0	29.0	35.0	2.0	35.0
100-101	28.33535249173034	34.0	29.0	35.0	2.0	35.0
102-103	27.464579260362356	32.5	27.5	34.5	2.0	35.0
104-105	28.07997730624525	34.0	29.0	35.0	2.0	35.0
106-107	28.172022221094437	34.0	29.0	35.0	2.0	35.0
108-109	28.117242481557536	34.0	29.0	35.0	2.0	35.0
110-111	28.033343382414763	34.0	29.0	35.0	2.0	35.0
112-113	27.944419616311933	34.0	29.0	35.0	2.0	35.0
114-115	27.818013281451794	34.0	29.0	35.0	2.0	35.0
116-117	27.67760324863069	34.0	28.5	35.0	2.0	35.0
118-119	27.674854395627285	34.0	28.0	35.0	2.0	35.0
120-121	27.849559191235784	34.0	29.0	35.0	2.0	35.0
122-123	27.714586266945517	34.0	29.0	35.0	2.0	35.0
124-125	27.100360024754067	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69952.0
3	8919.0
4	5701.0
5	4703.0
6	4725.0
7	5336.0
8	5736.0
9	5835.0
10	6399.0
11	6858.0
12	6980.0
13	7028.0
14	7464.0
15	6729.0
16	5632.0
17	4908.0
18	4540.0
19	4307.0
20	4237.0
21	4381.0
22	4880.0
23	6202.0
24	8819.0
25	10997.0
26	12072.0
27	13492.0
28	16088.0
29	19126.0
30	23050.0
31	29162.0
32	38439.0
33	52522.0
34	80998.0
35	129131.0
36	249982.0
37	360295.0
38	84515.0
39	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56984949293073	18.462912651957893	12.896590597437152	25.07064725767422
2	15.940158119774669	20.01610591418866	39.381460168543924	24.66227579749275
3	20.13349706710989	24.26492145248324	28.34377123532892	27.257810245077952
4	13.47005257655419	15.308565281292141	34.8411339565751	36.380248185578566
5	14.219906535896715	38.350507810517406	32.28617378202365	15.143411871562233
6	33.66251558077947	34.32598211004482	17.436833909837958	14.574668399337746
7	29.32388072930911	32.37830012219799	19.484783585317757	18.81303556317515
8	27.692440560245817	35.16687979927151	18.77155316092825	18.36912647955442
9	27.925769253495712	16.33980937027952	18.34867875816449	37.38574261806028
10-11	26.39455205137053	26.00937914399876	26.364212440906886	21.231856363723825
12-13	25.841030485342902	23.574533909764355	26.81275410449542	23.771681500397328
14-15	23.741860366461527	24.851373094439445	24.07021068356256	27.336555855536464
16-17	24.071665457167835	27.776481585569645	24.600486627588996	23.551366329673527
18-19	24.00351340213683	27.212011623467525	26.014742956530167	22.769732017865476
20-21	24.908112882528417	25.93129449480266	25.95311105690638	23.207481565762542
22-23	24.921700681276985	25.401628444024272	25.820765104316397	23.855905770382346
24-25	24.331739879561106	25.67426350581798	25.91833805082008	24.075658563800836
26-27	23.911642077769393	26.027188684562176	26.01855302747957	24.042616210188864
28-29	24.073855934113258	26.456356738039677	25.89559675581128	23.574190572035782
30-31	24.746136079394166	25.87022014291374	25.860486009740193	23.5231577679519
32-33	24.371361658657534	25.909516683200064	25.849102556672804	23.870019101469598
34-35	24.324688763675535	26.18835687522821	25.939352664807153	23.547601696289103
36-37	24.281674990104673	26.16517715950131	25.99469345317569	23.558454397218323
38-39	24.266554046376328	26.340118327058416	25.800146961903536	23.593180664661723
40-41	24.315961714006505	26.140303697166427	25.851340653679934	23.692393935147134
42-43	24.04164173112032	26.00358314331816	25.832077086821155	24.12269803874037
44-45	23.895946190610022	26.057541018751323	26.055609298618933	23.990903492019726
46-47	23.855904148213916	25.939707796290712	26.050803082941552	24.153584972553823
48-49	23.782549804780714	26.236237305443566	25.998330371333296	23.982882518442427
50-51	23.85590230366782	26.247745893179292	26.045373404323414	23.850978398829476
52-53	23.955065338125415	26.112107883185637	25.891403986608534	24.041422792080418
54-55	23.709429206993946	25.922242098895943	26.157643696293686	24.21068499781643
56-57	23.86631572448488	26.079236219717885	25.79656085247285	24.25788720332438
58-59	23.867102099440483	26.2580052815541	26.011085809345897	23.863806809659515
60-61	24.258065884552423	26.18599239298324	26.005664735242938	23.550276987221395
62-63	24.03619297313328	26.12972368182333	25.99204372730506	23.84203961773833
64-65	23.889684271126868	25.88794958034118	25.978812532194045	24.24355361633791
66-67	23.931381787661085	26.14258898460438	25.80379300000909	24.12223622772544
68-69	23.680049088027026	26.014987756400664	26.051046245390896	24.25391691018141
70-71	23.68585214276597	25.942643177112114	26.223189149040856	24.148315531081064
72-73	23.756678405560734	26.363008318227305	25.816199067807073	24.064114208404884
74-75	23.668385579313963	26.25205710855284	25.940872687469106	24.13868462466409
76-77	23.61792714922512	25.88304227793225	26.001214293208253	24.497816279634378
78-79	23.56556468977428	25.93401016189724	26.169669217556635	24.33075593077184
80-81	23.789792790209795	26.192387069357654	25.836135760306423	24.181684380126132
82-83	23.84804735717984	26.167690140599674	26.098452725579325	23.88580977664116
84-85	23.866766007203577	26.18529686823528	25.804701908315554	24.143235216245586
86-87	23.497069475808498	26.45925863078577	25.86247254882503	24.18119934458071
88-89	23.45437542681248	26.064583474578356	26.099315547128622	24.381725551480546
90-91	23.786727874266862	25.968044027051036	26.097390533653765	24.14783756502834
92-93	23.929231843776442	25.993915414258627	25.998801566902035	24.078051175062896
94-95	23.804201218193434	25.921476104099067	25.977685265813655	24.296637411893844
96-97	23.45412232783326	25.873361701096098	26.130663925341825	24.541852045728813
98-99	23.31506913653756	25.92902340710978	25.99398213275215	24.761925323600508
100-101	23.33982044660328	26.16750994088874	26.076036340965096	24.416633271542885
102-103	23.15352304190229	26.166791026403402	26.149330161844414	24.530355769849894
104-105	23.28776877596858	26.164130883528202	26.042473894122057	24.50562644638116
106-107	23.339147429960967	25.99791298551021	26.17919159436923	24.483747990159593
108-109	23.199887885370366	26.053924864259866	26.145510396169925	24.60067685419985
110-111	23.106729335369096	26.50353562536954	26.009354853005178	24.380380186256193
112-113	23.022600207484516	26.493212438314263	26.131989393215733	24.352197960985485
114-115	23.09227395475497	26.572573467308512	26.019376659382115	24.3157759185544
116-117	22.924764056791506	26.45343970693729	26.122713951006226	24.49908228526498
118-119	22.901351793248544	26.20079103083624	26.234174261984013	24.6636829139312
120-121	22.831079458463982	26.275337626692867	26.432759126166737	24.460823788676407
122-123	22.85323312234689	26.36405160633505	26.216530267818456	24.56618500349961
124-125	22.724065460470378	26.254066282343253	26.184453270845747	24.837414986340626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	483.0
1	386.0
2	256.5
3	207.5
4	182.5
5	155.0
6	150.0
7	201.0
8	289.0
9	353.5
10	359.5
11	365.0
12	389.5
13	414.5
14	450.0
15	505.0
16	576.5
17	608.5
18	632.5
19	705.5
20	765.5
21	849.5
22	984.5
23	1163.5
24	1441.0
25	1844.0
26	2429.0
27	3191.5
28	4072.0
29	5227.5
30	6742.5
31	8500.0
32	10706.0
33	13140.5
34	15975.5
35	18780.5
36	22053.0
37	25601.5
38	28035.0
39	30672.0
40	33552.0
41	36169.5
42	38600.0
43	41483.5
44	44667.5
45	47419.0
46	50540.5
47	53507.5
48	55853.0
49	58341.5
50	59551.5
51	59225.5
52	58547.5
53	57404.5
54	55575.5
55	52938.0
56	49714.5
57	45492.0
58	40811.0
59	35754.0
60	30155.0
61	24922.0
62	20051.0
63	15447.5
64	11480.5
65	8462.0
66	6297.0
67	4703.5
68	3532.5
69	2696.5
70	2048.5
71	1532.5
72	1141.5
73	835.0
74	616.5
75	412.0
76	276.5
77	196.0
78	127.5
79	107.0
80	79.0
81	49.0
82	43.0
83	38.0
84	28.5
85	24.5
86	20.5
87	17.5
88	15.0
89	12.5
90	14.0
91	13.5
92	9.0
93	6.0
94	4.0
95	4.0
96	5.5
97	4.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018860964393680592
2	0.013255697867044593
3	0.011968001502817403
4	0.015679596905589892
5	0.008180659255090377
6	0.03423757391945232
7	0.014997875300999023
8	0.014770634766135403
9	0.010680305138590215
10-11	0.009884963266567539
12-13	0.008332152944999458
14-15	0.007801925030317674
16-17	0.006135494441317783
18-19	0.007498937650499512
20-21	0.0067414692009541075
22-23	0.007006583158294999
24-25	0.0074610642280222425
26-27	0.0059840007514087015
28-29	0.007839798452794946
30-31	0.006817216045908648
32-33	0.009960710111522079
34-35	0.00977134299913573
36-37	0.009051747972067594
38-39	0.008862380859681242
40-41	0.009241115084453945
42-43	0.008862380859681242
44-45	0.009089621394544865
46-47	0.01128627989822654
48-49	0.01007433037895389
50-51	0.007309570538113161
52-53	0.007006583158294999
54-55	0.007764051607840405
56-57	0.009203241661976676
58-59	0.009165368239499403
60-61	0.006817216045908648
62-63	0.007309570538113161
64-65	0.005075038611954215
66-67	0.005378025991772378
68-69	0.008294279522522189
70-71	0.006249114708749593
72-73	0.005605266526635999
74-75	0.0052644057243405665
76-77	0.005870380483976891
78-79	0.004506937274795161
80-81	0.003900962515158837
82-83	0.006930836313340458
84-85	0.011248406475749269
86-87	0.008786634014726702
88-89	0.006173367863795054
90-91	0.0067793426234313775
92-93	0.009847089844090268
94-95	0.008900254282158512
96-97	0.010907545673453837
98-99	0.008938127704635783
100-101	0.01015007722390843
102-103	0.006968709735817729
104-105	0.006211241286272324
106-107	0.009051747972067594
108-109	0.008294279522522189
110-111	0.009354735351885756
112-113	0.0067035957784768365
114-115	0.003181367488090702
116-117	0.004544810697272432
118-119	0.16361318510180756
120-121	0.012422482572544647
122-123	0.0029541269532270807
124-125	0.002386025616068027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1320187.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.50903863104085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.17278425363351	60.90623919506387
2	8.831080130373943	12.63004100393455
3	2.516744043517087	5.399098410969159
4	1.1464871362634592	3.2793677166822044
5	0.6215533672629961	2.2223341875431557
6	0.38748041633235064	1.6625011236169114
7	0.26972021837703686	1.350120346084741
8	0.18535227952478192	1.060349065351129
9	0.1341756113604682	0.863529207850732
>10	0.7048717209272255	8.645908902048209
>50	0.02435254052469156	1.1848510933697844
>100	0.004869050141672521	0.5488356069067569
>500	5.292317608012568E-4	0.2468241405788345
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.574684495454053E-5	0.0
5	0.0	0.0	0.0	7.574684495454053E-5	0.0
6	0.0	0.0	0.0	1.5149368990908106E-4	0.0
7	0.0	0.0	0.0	1.5149368990908106E-4	0.0
8	0.0	0.0	0.0	1.5149368990908106E-4	0.0
9	0.0	0.0	0.0	1.5149368990908106E-4	7.574684495454053E-5
10-11	0.0	0.0	0.0	1.5149368990908106E-4	7.574684495454053E-5
12-13	0.0	0.0	0.0	1.5149368990908106E-4	7.574684495454053E-5
14-15	0.0	0.0	0.0	1.5149368990908106E-4	1.8936711238635133E-4
16-17	0.0	0.0	0.0	1.5149368990908106E-4	2.272405348636216E-4
18-19	0.0	0.0	0.0	2.272405348636216E-4	2.272405348636216E-4
20-21	0.0	0.0	0.0	2.272405348636216E-4	2.272405348636216E-4
22-23	0.0	0.0	0.0	3.4086080229543236E-4	2.272405348636216E-4
24-25	0.0	0.0	0.0	5.302279146817837E-4	2.6511395734089187E-4
26-27	0.0	0.0	0.0	8.332152944999459E-4	3.7873422477270266E-4
28-29	0.0	0.0	0.0	0.002120911658727135	4.1660764724997296E-4
30-31	0.0	0.0	0.0	0.01003645695647662	4.544810697272432E-4
32-33	0.0	0.0	0.0	0.028064206055657268	4.544810697272432E-4
34-35	0.0	0.0	0.0	0.04968993029017858	4.544810697272432E-4
36-37	0.0	0.0	0.0	0.0799886682719948	4.544810697272432E-4
38-39	0.0	0.0	0.0	0.12975434540712794	4.544810697272432E-4
40-41	0.0	0.0	0.0	0.18546614987119248	5.302279146817837E-4
42-43	0.0	0.0	0.0	0.23882980214166633	5.302279146817837E-4
44-45	0.0	0.0	0.0	0.2958671763924353	5.302279146817837E-4
46-47	0.0	0.0	0.0	0.359305159041863	5.68101337159054E-4
48-49	0.0	0.0	0.0	0.42664410420644955	6.059747596363242E-4
50-51	0.0	0.0	0.0	0.5001942906573084	6.059747596363242E-4
52-53	0.0	0.0	0.0	0.5811676679137122	6.059747596363242E-4
54-55	0.0	0.0	0.0	0.6650572987008658	6.059747596363242E-4
56-57	0.0	0.0	0.0	0.7566731076733826	6.438481821135945E-4
58-59	0.0	0.0	0.0	0.8510536764867401	6.817216045908648E-4
60-61	0.0	0.0	0.0	0.9469113087767111	6.817216045908648E-4
62-63	0.0	0.0	0.0	1.0539416006974769	6.817216045908648E-4
64-65	0.0	0.0	0.0	1.1652894627806516	7.19595027068135E-4
66-67	0.0	0.0	0.0	1.2930365167964841	7.574684495454053E-4
68-69	0.0	0.0	0.0	1.4366904082527703	7.574684495454053E-4
70-71	0.0	0.0	0.0	1.5832984266622834	7.574684495454053E-4
72-73	0.0	0.0	0.0	1.7238845708979107	8.332152944999458E-4
74-75	0.0	0.0	0.0	1.875113146849651	8.332152944999458E-4
76-77	0.0	0.0	0.0	2.0465282569817758	8.332152944999458E-4
78-79	0.0	0.0	0.0	2.2429019525264224	8.332152944999458E-4
80-81	0.0	0.0	0.0	2.4419267876444777	8.710887169772161E-4
82-83	0.0	0.0	0.0	2.6408001290726237	9.847089844090269E-4
84-85	0.0	0.0	0.0	2.8472860284187016	9.847089844090269E-4
86-87	0.0	0.0	0.0	3.0814573995956636	9.847089844090269E-4
88-89	0.0	0.0	0.0	3.343541483138374	9.847089844090269E-4
90-91	0.0	0.0	0.0	3.6131245043315836	9.847089844090269E-4
92-93	0.0	0.0	0.0	3.8876689438693157	9.847089844090269E-4
94-95	0.0	0.0	0.0	4.16399343426348	0.0010604558293635675
96-97	0.0	0.0	0.0	4.481183347510617	0.0010604558293635675
98-99	0.0	0.0	0.0	4.835110480560708	0.0010604558293635675
100-101	0.0	0.0	0.0	5.206383641105389	0.0010604558293635675
102-103	0.0	0.0	0.0	5.584701258230842	0.0011362026743181079
104-105	0.0	0.0	0.0	5.991840549861497	0.0011362026743181079
106-107	0.0	0.0	0.0	6.428483237601945	0.0011362026743181079
108-109	0.0	0.0	0.0	6.883683902356257	0.0011362026743181079
110-111	0.0	0.0	0.0	7.37770482514977	0.0011362026743181079
112-113	0.0	0.0	0.0	7.86983207681942	0.0011362026743181079
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1520	0.0	55.603886	1
GTATCAA	2495	0.0	47.472584	1
GTACATG	7485	0.0	39.36169	1
TACATGG	7785	0.0	37.839127	2
TCAACGC	3150	0.0	37.40668	4
ACATGGG	8090	0.0	36.265438	3
CAACGCA	3275	0.0	35.978947	5
ATCAACG	3350	0.0	35.528732	3
CATGGGG	6815	0.0	34.75456	4
AACGCAG	3495	0.0	33.88188	6
TATCAAC	3465	0.0	33.834328	2
ATGGGGG	4145	0.0	33.021576	5
GTGGTAT	920	0.0	27.172014	1
ACGCAGA	4405	0.0	26.74128	7
TGGGGGG	3035	0.0	26.468948	6
CGCAGAG	4580	0.0	25.849401	8
GCAGAGT	5135	0.0	22.822975	9
AGAGTAC	4545	0.0	22.447102	10-11
TGGTATC	975	0.0	21.973154	2
GAGTACA	5220	0.0	21.892284	1
>>END_MODULE