FastQCFastQC Report
Mon 15 Aug 2016
SRR2921315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320187
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24290.18398908639457895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.1366473082979911No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17090.12945135802730978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.062.2698971
GTATCAA38750.051.824621
TATCAAC52200.038.2491462
TCAACGC51200.037.366514
CAACGCA52150.037.1429565
ATCAACG52750.036.7204743
GTACATG73400.035.5839351
AACGCAG54600.035.47096
TACATGG75350.034.565722
ACATGGG78200.033.5346153
CATGGGG65100.032.3177534
ATGGGGG39700.030.4756455
GTATACG1004.323675E-429.8839911
ACGCAGA66100.029.0193487
CGCAGAG69150.027.561878
GTGGTAT11750.027.4678361
TGGTATC12450.025.3719522
TGGGGGG31100.024.9094686
GTAAGTA7950.024.7396872
GCAGAGT75850.023.8709059
Produced by FastQC (version 0.11.5)