FastQCFastQC Report
Mon 15 Aug 2016
SRR2921314_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921314_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536572
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24100.052.349811
GGTATCA14150.052.150871
GTACATG81200.042.0680351
TACATGG84350.040.210892
TCAACGC30900.039.8613174
CAACGCA31400.039.4160845
ACATGGG89050.037.7544753
CATGGGG76800.037.344414
ATCAACG33350.037.1126023
AACGCAG33400.036.6983386
TATCAAC34550.035.8235932
ATGGGGG51350.035.574415
TGGGGGG38450.029.2476566
ACGCAGA42800.028.4947627
GTATAGG4100.027.578211
CGCAGAG45850.026.7290088
GAGTACA54800.023.5654351
ATGGGGA25000.023.5632385
GCAGAGT52800.023.4360499
AGTACAT54050.023.009362
Produced by FastQC (version 0.11.5)