FastQCFastQC Report
Mon 15 Aug 2016
SRR2921314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536572
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.11727403597097956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19650.061.4488531
GTATCAA33050.050.099491
GTACATG79400.036.9645961
TACATGG83400.035.0192762
TCAACGC46350.034.7209134
ACATGGG84400.034.463113
TATCAAC48700.033.9022032
CATGGGG74350.033.2693444
CAACGCA47900.033.224075
AACGCAG50300.032.114916
ATCAACG50850.031.8826983
ATGGGGG46450.030.2833145
GTGGTAT10900.028.5168691
ACGCAGA62650.025.6823567
TGGTATC11400.025.6193622
TGGGGGG34850.024.9721266
CGCAGAG65300.024.4544098
GTATAGG4200.024.1949421
GTAAGTA8200.023.2601912
AGAGTAC65050.022.80504810-11
Produced by FastQC (version 0.11.5)