##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921314_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1536572 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.166598766605144 31.0 30.0 34.0 18.0 34.0 2 29.942955487930277 33.0 31.0 34.0 26.0 34.0 3 30.18347138956066 33.0 31.0 34.0 26.0 34.0 4 32.32564565799715 37.0 35.0 37.0 19.0 37.0 5 32.00068594247455 37.0 33.0 37.0 19.0 37.0 6 32.159404831013454 37.0 35.0 37.0 17.0 37.0 7 32.380408467680006 37.0 35.0 37.0 17.0 37.0 8 32.63347958963199 37.0 35.0 37.0 17.0 37.0 9 34.35366582236303 39.0 37.0 39.0 17.0 39.0 10-11 34.5432739240335 39.0 37.0 39.0 22.0 39.0 12-13 34.57692545484363 39.0 37.0 39.0 24.5 39.0 14-15 35.978525249711694 40.5 38.0 41.0 25.0 41.0 16-17 36.000925111221605 41.0 38.0 41.0 25.0 41.0 18-19 36.02148874247351 41.0 38.0 41.0 25.0 41.0 20-21 36.00607944177038 41.0 38.0 41.0 25.0 41.0 22-23 35.971193019266266 41.0 38.0 41.0 25.0 41.0 24-25 35.91708035809582 41.0 38.0 41.0 25.0 41.0 26-27 35.829089362555095 40.5 38.0 41.0 24.0 41.0 28-29 35.75037941599873 40.0 37.0 41.0 24.0 41.0 30-31 35.6558654589567 40.0 37.0 41.0 23.5 41.0 32-33 35.52533724420333 40.0 37.0 41.0 22.0 41.0 34-35 35.44481775016075 40.0 37.0 41.0 21.5 41.0 36-37 35.36577589595541 40.0 36.0 41.0 19.5 41.0 38-39 35.267489906102675 40.0 36.0 41.0 18.5 41.0 40-41 35.13647652046243 40.0 36.0 41.0 17.0 41.0 42-43 34.99374581861443 40.0 35.0 41.0 15.0 41.0 44-45 34.84634888570142 40.0 35.0 41.0 11.0 41.0 46-47 34.63654940998535 40.0 35.0 41.0 7.5 41.0 48-49 34.423557112846 40.0 35.0 41.0 2.0 41.0 50-51 34.23361319873068 40.0 35.0 41.0 2.0 41.0 52-53 34.04757408048565 39.0 35.0 41.0 2.0 41.0 54-55 33.84420417656966 39.0 35.0 41.0 2.0 41.0 56-57 33.60170496403683 39.0 34.5 41.0 2.0 41.0 58-59 33.532434536097234 38.5 35.0 41.0 2.0 41.0 60-61 33.28673274015145 37.5 34.0 41.0 2.0 41.0 62-63 32.9863921117917 37.0 34.0 40.0 2.0 41.0 64-65 32.67992681110941 36.5 34.0 40.0 2.0 41.0 66-67 32.363803648641266 36.0 34.0 39.0 2.0 41.0 68-69 32.025225632121376 35.5 34.0 39.0 2.0 41.0 70-71 31.698246486334515 35.0 33.0 38.5 2.0 40.5 72-73 31.353097349164244 35.0 33.0 37.0 2.0 39.5 74-75 31.023581387660325 35.0 33.0 37.0 2.0 39.0 76-77 30.7290309207769 35.0 33.0 36.0 2.0 39.0 78-79 30.47588332990579 35.0 33.0 36.0 2.0 37.0 80-81 30.25530108579357 35.0 32.5 35.5 2.0 37.0 82-83 30.063056270711687 35.0 32.0 35.0 2.0 36.5 84-85 29.895587060027125 35.0 32.0 35.0 2.0 36.0 86-87 29.76382915997428 35.0 32.0 35.0 2.0 36.0 88-89 29.652711034692807 35.0 32.0 35.0 2.0 36.0 90-91 29.55712325878644 35.0 32.0 35.0 2.0 35.0 92-93 29.48849712216544 35.0 32.0 35.0 2.0 35.0 94-95 29.422782987064714 35.0 32.0 35.0 2.0 35.0 96-97 29.35899977352184 35.0 32.0 35.0 2.0 35.0 98-99 29.303567291347232 35.0 32.0 35.0 2.0 35.0 100-101 29.247892061029354 35.0 31.0 35.0 2.0 35.0 102-103 28.579313888317632 34.0 29.5 35.0 2.0 35.0 104-105 29.120466531994595 35.0 31.0 35.0 2.0 35.0 106-107 29.1770349843678 35.0 31.0 35.0 2.0 35.0 108-109 29.24717618178647 35.0 31.5 35.0 2.0 35.0 110-111 29.358258513105795 35.0 32.0 35.0 2.0 35.0 112-113 29.32102953847916 35.0 32.0 35.0 2.0 35.0 114-115 29.263304941128695 35.0 32.0 35.0 2.0 35.0 116-117 29.19821004157306 35.0 32.0 35.0 2.0 35.0 118-119 29.122856592466867 35.0 32.0 35.0 2.0 35.0 120-121 29.02670099416103 35.0 32.0 35.0 2.0 35.0 122-123 28.915700988954633 35.0 32.0 35.0 2.0 35.0 124-125 28.395508638710062 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103918.0 3 6287.0 4 4786.0 5 4529.0 6 4765.0 7 5080.0 8 5313.0 9 5201.0 10 5298.0 11 5335.0 12 5886.0 13 6942.0 14 8579.0 15 7426.0 16 5046.0 17 4348.0 18 3929.0 19 3585.0 20 3442.0 21 3482.0 22 3987.0 23 4892.0 24 6669.0 25 8486.0 26 10332.0 27 11594.0 28 13857.0 29 16566.0 30 19941.0 31 24655.0 32 31695.0 33 43016.0 34 66047.0 35 111360.0 36 252032.0 37 532238.0 38 175789.0 39 239.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4893299074784 17.684517426010807 11.946493440672143 26.879659225838644 2 16.05495870027568 19.093215287015514 38.22150865693244 26.630317355776366 3 19.524174591232953 22.90559765503992 28.151690906771698 29.41853684695543 4 14.151389837260513 15.56820822530576 30.646785470418724 39.633616467015 5 14.677829505887876 37.598946311438 31.857712922029656 15.865511260644471 6 34.84776267125071 33.73254935667031 16.000026045736313 15.419661926342657 7 30.984362326369226 31.460418888112063 18.16019404203463 19.395024743484086 8 28.348350850153732 35.83591290968402 17.105650248498467 18.71008599166378 9 28.280990778086352 16.306496343191345 17.3478586012292 38.064654277493105 10-11 27.524508192827067 25.531962666454934 24.869494646042668 22.074034494675328 12-13 26.991843977404073 22.672603252641345 25.860061536457906 24.475491233496673 14-15 24.68859252934454 23.928035913709216 23.267800011974707 28.115571544971534 16-17 24.738606456449812 27.024278719122822 23.78717040268858 24.449944421738778 18-19 24.738053277034854 26.0290438716832 25.544068224593445 23.6888346266885 20-21 25.494054297488173 25.071067284839238 25.515530674774755 23.919347742897827 22-23 25.30284945970641 24.4300625027659 25.4908003009296 24.77628773659809 24-25 24.82919772064049 24.77856553418909 25.461254012177758 24.930982732992664 26-27 24.44987934180761 25.026259752227688 25.615005349570342 24.908855556394364 28-29 24.57096055375212 25.46157941183361 25.504109146854166 24.4633508875601 30-31 25.28732789612202 25.090005544810133 25.40092491598181 24.22174164308604 32-33 24.85893924918585 25.126710625990846 25.42751006786535 24.586840056957957 34-35 24.874623512598173 25.346225233832193 25.59391945187079 24.185231801698848 36-37 24.959162343189906 25.088508706393192 25.658316043764952 24.29401290665195 38-39 24.768543224788687 25.40531781133588 25.536095933024942 24.29004303085049 40-41 24.817776192719897 25.239949706229194 25.578788367873422 24.36348573317749 42-43 24.592697250763386 25.216748710766563 25.355791983714397 24.83476205475565 44-45 24.32238775664271 25.29198111120078 25.73045714746852 24.655173984687995 46-47 24.345816531864436 25.29751290535035 25.280429423417843 25.076241139367372 48-49 24.50109724763955 25.565186662258586 25.02606451243417 24.907651577667693 50-51 24.438913373405217 25.645039737805973 25.24636007944958 24.669686809339233 52-53 24.640042900690627 25.490409821342574 25.111254142337618 24.75829313562918 54-55 24.28714697391336 25.172689597363483 25.542734086004433 24.99742934271873 56-57 24.449911881773193 25.433952981051327 25.09814053620657 25.017994600968912 58-59 24.411840122037887 25.419863175952706 25.430764064423926 24.73753263758548 60-61 24.875437011737816 25.285896137636243 25.44303163144975 24.395635219176192 62-63 24.654848585032134 25.477426375073865 25.282739760974426 24.584985278919568 64-65 24.46741838325832 25.10747950632967 25.32862111245031 25.096480997961695 66-67 24.36905006729265 25.351366548394736 25.26653485811273 25.013048526199878 68-69 24.139317910257375 25.297447825419177 25.49460747690313 25.06862678742031 70-71 24.19740174882791 25.240372725781807 25.497698773633775 25.064526751756507 72-73 24.311909887724102 25.6946957252898 24.97142991021573 25.02196447677037 74-75 24.175860291610153 25.636742046581613 25.139205972775763 25.048191689032468 76-77 24.211133614305087 25.13328369903916 25.204058124188126 25.45152456246762 78-79 24.15946014895495 25.210696277167617 25.444268150142 25.185575423735436 80-81 24.39739237731782 25.432228362875282 25.06686962927868 25.103509630528215 82-83 24.484989964674615 25.43398552101691 25.150660040661943 24.93036447364653 84-85 24.321086158019277 25.627435616424094 24.906219819181917 25.145258406374708 86-87 23.932558968925637 25.83312073889151 25.091144443605636 25.143175848577222 88-89 23.835069232030783 25.525455364278404 25.18590082339129 25.453574580299527 90-91 24.230776865173098 25.231937569900165 25.19982050890736 25.337465056019376 92-93 24.1759289479413 25.46090675718901 25.154866687904537 25.208297606965154 94-95 24.23185268752719 25.36662001515713 24.958405748385772 25.443121548929902 96-97 23.868612046097656 25.198299582705307 25.178840581973805 25.75424778922323 98-99 23.781743967007067 25.363577288788708 25.038730706641378 25.815948037562848 100-101 23.83415811299438 25.484747867330654 25.164717305794976 25.516376713879986 102-103 23.682554413330454 25.36200711714127 25.16234188830722 25.793096581221054 104-105 23.81438156310982 25.461164677335223 25.01897894110362 25.70547481845134 106-107 23.876669384843765 25.286238366126092 25.160894173968106 25.67619807506204 108-109 23.844530842230903 25.27265414607416 25.149782436374192 25.733032575320745 110-111 23.61112964091624 25.679641615852518 25.134236235599765 25.574992507631478 112-113 23.60240746658267 25.6994714793437 25.110165415706454 25.587955638367177 114-115 23.612408196203873 25.772909053635868 24.989570893518987 25.625111856641276 116-117 23.395730451737524 25.5694878676729 25.079040399438746 25.955741281150836 118-119 23.52795894480255 25.400479185560265 25.174260387858503 25.89730148177868 120-121 23.456897384859733 25.3672543060964 25.328629354377586 25.84721895466628 122-123 23.391882323233638 25.59318029765422 25.031401557256917 25.983535821855224 124-125 23.176052391981887 25.585410631854955 24.86744836392201 26.37108861224115 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 33.0 1 37.5 2 60.5 3 71.0 4 60.0 5 90.0 6 153.0 7 365.0 8 551.5 9 619.5 10 616.0 11 546.0 12 584.5 13 651.0 14 701.0 15 750.0 16 823.5 17 890.0 18 950.0 19 1027.5 20 1100.5 21 1170.0 22 1248.0 23 1356.0 24 1544.5 25 1752.5 26 2113.5 27 2618.5 28 3164.0 29 3982.5 30 5070.0 31 6422.0 32 8328.0 33 10428.5 34 12689.0 35 15200.5 36 18030.5 37 21230.0 38 24191.0 39 27462.5 40 30990.0 41 33968.0 42 36880.0 43 40854.5 44 45364.0 45 50082.0 46 54840.0 47 59187.5 48 64070.0 49 68925.0 50 72371.0 51 75070.0 52 75606.0 53 74492.0 54 73573.5 55 71569.0 56 67544.5 57 61415.0 58 55481.0 59 49391.5 60 42473.5 61 35931.5 62 29316.0 63 22430.0 64 16436.0 65 12017.0 66 8958.0 67 6750.0 68 5097.0 69 3952.5 70 2940.5 71 2190.0 72 1621.5 73 1247.0 74 973.0 75 683.0 76 521.0 77 392.0 78 295.0 79 235.5 80 197.0 81 160.0 82 139.5 83 134.5 84 117.0 85 108.0 86 108.0 87 96.0 88 88.5 89 86.0 90 74.0 91 67.0 92 65.5 93 73.0 94 68.0 95 53.0 96 49.0 97 41.5 98 29.0 99 13.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3559872235079124 2 0.0 3 0.0 4 0.020370018456668477 5 0.18951275957130548 6 0.052844904111229406 7 0.026682771780300565 8 0.009371510088690931 9 0.00800483153409017 10-11 0.002928596902715916 12-13 0.05837669826080392 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.579396214430564E-4 92-93 5.531794149574508E-4 94-95 9.761989675719719E-5 96-97 5.206394493717184E-4 98-99 3.25399655857324E-5 100-101 0.0 102-103 0.0 104-105 3.25399655857324E-5 106-107 1.9523979351439438E-4 108-109 6.50799311714648E-4 110-111 5.531794149574508E-4 112-113 0.0012365186922578312 114-115 4.5555951820025356E-4 116-117 0.0010412788987434367 118-119 4.2301955261452116E-4 120-121 3.25399655857324E-5 122-123 0.001561918348115155 124-125 6.50799311714648E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1536572.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.64415332376764 #Duplication Level Percentage of deduplicated Percentage of total 1 82.50917247423297 52.512264235672724 2 9.346130228394037 11.89653090479619 3 3.0743178939142415 5.869870782188412 4 1.471766566418475 3.746773480397299 5 0.8991618480741219 2.861319726085584 6 0.5505503684395673 2.1023587236854753 7 0.40054005950851446 1.7844423071769637 8 0.30315990524085085 1.5435484392614063 9 0.2290731945209539 1.3121252563011154 >10 1.15652661283494 12.843007552223531 >50 0.04784338540259234 2.0228539058586845 >100 0.011144098063268526 1.0426404402564675 >500 2.044549851410802E-4 0.09394927112776084 >1k 4.089099702821604E-4 0.36831497496832005 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1802 0.11727403597097956 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.50799311714648E-5 2 0.0 0.0 0.0 0.0 6.50799311714648E-5 3 0.0 0.0 0.0 0.0 6.50799311714648E-5 4 0.0 0.0 0.0 0.0 6.50799311714648E-5 5 0.0 0.0 0.0 0.0 6.50799311714648E-5 6 0.0 0.0 0.0 0.0 6.50799311714648E-5 7 0.0 0.0 0.0 0.0 6.50799311714648E-5 8 0.0 0.0 0.0 0.0 6.50799311714648E-5 9 0.0 0.0 0.0 0.0 6.50799311714648E-5 10-11 0.0 0.0 0.0 0.0 6.50799311714648E-5 12-13 0.0 0.0 0.0 0.0 6.50799311714648E-5 14-15 0.0 0.0 0.0 0.0 1.62699827928662E-4 16-17 6.50799311714648E-5 0.0 0.0 0.0 2.603197246858592E-4 18-19 6.50799311714648E-5 0.0 0.0 0.0 3.2539965585732394E-4 20-21 6.50799311714648E-5 0.0 0.0 0.0 3.2539965585732394E-4 22-23 6.50799311714648E-5 0.0 0.0 6.50799311714648E-5 3.9047958702878875E-4 24-25 6.50799311714648E-5 0.0 0.0 1.9523979351439438E-4 4.5555951820025356E-4 26-27 6.50799311714648E-5 0.0 0.0 2.603197246858592E-4 5.206394493717184E-4 28-29 6.50799311714648E-5 0.0 0.0 0.0018547780383867465 5.206394493717184E-4 30-31 6.50799311714648E-5 0.0 0.0 0.009566749882205326 5.206394493717184E-4 32-33 6.50799311714648E-5 0.0 0.0 0.02547879305362847 5.857193805431832E-4 34-35 6.50799311714648E-5 0.0 0.0 0.0440916533686674 7.158792428861127E-4 36-37 6.50799311714648E-5 0.0 0.0 0.07236888346266884 7.158792428861127E-4 38-39 6.50799311714648E-5 0.0 0.0 0.12121137180685318 7.158792428861127E-4 40-41 6.50799311714648E-5 0.0 0.0 0.185315104010746 7.158792428861127E-4 42-43 6.50799311714648E-5 0.0 0.0 0.24073066540324828 7.158792428861127E-4 44-45 6.50799311714648E-5 0.0 0.0 0.2974803653847655 7.158792428861127E-4 46-47 6.50799311714648E-5 0.0 0.0 0.3555967439208836 7.484192084718451E-4 48-49 6.50799311714648E-5 0.0 0.0 0.4233774922359642 7.809591740575775E-4 50-51 6.50799311714648E-5 0.0 0.0 0.5008226103300073 7.809591740575775E-4 52-53 6.50799311714648E-5 0.0 0.0 0.5846781016444397 7.809591740575775E-4 54-55 6.50799311714648E-5 6.50799311714648E-5 0.0 0.6703558310316731 7.809591740575775E-4 56-57 6.50799311714648E-5 6.50799311714648E-5 0.0 0.755480381003949 7.809591740575775E-4 58-59 6.50799311714648E-5 6.50799311714648E-5 0.0 0.8550201357307045 7.809591740575775E-4 60-61 6.50799311714648E-5 6.50799311714648E-5 0.0 0.9646147398234513 7.809591740575775E-4 62-63 6.50799311714648E-5 6.50799311714648E-5 0.0 1.09119520595195 9.761989675719719E-4 64-65 6.50799311714648E-5 6.50799311714648E-5 0.0 1.1973405736926093 9.761989675719719E-4 66-67 6.50799311714648E-5 6.50799311714648E-5 0.0 1.3158511283558467 9.761989675719719E-4 68-69 6.50799311714648E-5 6.50799311714648E-5 0.0 1.4537880424737661 9.761989675719719E-4 70-71 6.50799311714648E-5 6.50799311714648E-5 0.0 1.5995670882978472 0.0011063588299149016 72-73 6.50799311714648E-5 6.50799311714648E-5 0.0 1.7390008408327107 0.0013666785546007606 74-75 6.50799311714648E-5 6.50799311714648E-5 0.0 1.8783044335052312 0.0013666785546007606 76-77 6.50799311714648E-5 6.50799311714648E-5 0.0 2.0484233735874398 0.0013666785546007606 78-79 6.50799311714648E-5 6.50799311714648E-5 3.25399655857324E-5 2.236699614466488 0.0013666785546007606 80-81 6.50799311714648E-5 6.50799311714648E-5 6.50799311714648E-5 2.41784960288226 0.001399218520186493 82-83 6.50799311714648E-5 6.50799311714648E-5 6.50799311714648E-5 2.6068091830386084 0.0014317584857722254 84-85 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 2.8022767563121027 0.0014317584857722254 86-87 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 3.0194484866312807 0.0014317584857722254 88-89 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 3.2717959197486355 0.0014968384169436902 90-91 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 3.5342958221287386 0.0014968384169436902 92-93 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 3.787912313903937 0.0014968384169436902 94-95 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 4.033849373800902 0.0016920782104580846 96-97 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 4.324333646584735 0.0019523979351439439 98-99 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 4.662651668779596 0.0020174778663154085 100-101 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 5.016849194180292 0.0020174778663154085 102-103 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 5.382728567226268 0.002082557797486873 104-105 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 5.766244601619709 0.002147637728658338 106-107 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 6.167722697016476 0.0021801776942440704 108-109 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 6.590416849975139 0.0022777975910012677 110-111 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 7.041258073165462 0.0024404974189299296 112-113 6.50799311714648E-5 1.301598623429296E-4 6.50799311714648E-5 7.497728710402116 0.002538117315687127 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1965 0.0 61.448853 1 GTATCAA 3305 0.0 50.09949 1 GTACATG 7940 0.0 36.964596 1 TACATGG 8340 0.0 35.019276 2 TCAACGC 4635 0.0 34.720913 4 ACATGGG 8440 0.0 34.46311 3 TATCAAC 4870 0.0 33.902203 2 CATGGGG 7435 0.0 33.269344 4 CAACGCA 4790 0.0 33.22407 5 AACGCAG 5030 0.0 32.11491 6 ATCAACG 5085 0.0 31.882698 3 ATGGGGG 4645 0.0 30.283314 5 GTGGTAT 1090 0.0 28.516869 1 ACGCAGA 6265 0.0 25.682356 7 TGGTATC 1140 0.0 25.619362 2 TGGGGGG 3485 0.0 24.972126 6 CGCAGAG 6530 0.0 24.454409 8 GTATAGG 420 0.0 24.194942 1 GTAAGTA 820 0.0 23.260191 2 AGAGTAC 6505 0.0 22.805048 10-11 >>END_MODULE