FastQCFastQC Report
Mon 15 Aug 2016
SRR2921313_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921313_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316224
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21900.052.7171061
GGTATCA13050.050.1621441
TCAACGC24900.045.1672254
CAACGCA26350.042.4559145
TATCAAC27550.041.0386352
AACGCAG28100.040.232336
ATCAACG28500.039.2530983
GTACATG60800.038.6622771
TACATGG63350.036.6335452
ACATGGG64350.035.139533
CATGGGG57800.033.1504144
ACGCAGA36300.031.3043847
ATGGGGG36900.031.1237455
CGCAGAG37900.029.9816888
TACCGTA850.00721386927.9975137
TATACTG4750.027.5606845
GAGTACA43850.025.649931
GCAGAGT43800.025.1261839
AGTACAT43500.025.0335672
TGGGGGG27350.024.5838016
Produced by FastQC (version 0.11.5)