##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921313_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1316224
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.538363530827578	31.0	31.0	34.0	25.0	34.0
2	29.605564858261207	31.0	31.0	34.0	24.0	34.0
3	29.581935901487892	31.0	31.0	34.0	23.0	34.0
4	32.92459870052514	37.0	35.0	37.0	25.0	37.0
5	33.004150509335794	37.0	35.0	37.0	26.0	37.0
6	33.09090094087328	37.0	35.0	37.0	26.0	37.0
7	32.9554490724983	37.0	35.0	37.0	25.0	37.0
8	32.97170162525528	37.0	35.0	37.0	25.0	37.0
9	34.45945143075951	39.0	35.0	39.0	25.0	39.0
10-11	34.50128511560342	39.0	35.0	39.0	25.0	39.0
12-13	34.47769490603423	39.0	35.0	39.0	25.0	39.0
14-15	35.7095293050423	40.0	37.0	41.0	25.0	41.0
16-17	35.691432081469415	40.0	37.0	41.0	25.0	41.0
18-19	35.663076725542155	40.0	37.0	41.0	24.0	41.0
20-21	35.67443687396674	40.0	37.0	41.0	24.0	41.0
22-23	35.63806312603326	40.0	37.0	41.0	24.0	41.0
24-25	35.62081036358553	40.0	37.0	41.0	24.0	41.0
26-27	35.57603455035009	40.0	36.5	41.0	24.0	41.0
28-29	35.50350966096956	40.0	36.0	41.0	24.0	41.0
30-31	35.430147148205776	40.0	36.0	41.0	24.0	41.0
32-33	35.33296004327531	40.0	36.0	41.0	23.0	41.0
34-35	35.21458239630944	40.0	36.0	41.0	22.5	41.0
36-37	35.099736063162496	40.0	35.0	41.0	21.0	41.0
38-39	34.98939238305942	40.0	35.0	41.0	20.0	41.0
40-41	34.87054027278032	40.0	35.0	41.0	18.5	41.0
42-43	34.674588064037735	40.0	35.0	41.0	16.5	41.0
44-45	34.42973802331518	40.0	35.0	41.0	15.0	41.0
46-47	34.198524339322184	39.0	34.5	41.0	12.0	41.0
48-49	33.98371667740446	39.0	34.0	41.0	8.5	41.0
50-51	33.79948815703102	39.0	34.0	41.0	8.0	41.0
52-53	33.58822624416513	38.5	34.0	40.0	6.5	41.0
54-55	33.37526325306331	38.0	33.0	40.0	2.0	41.0
56-57	33.121915038777594	38.0	33.0	40.0	2.0	41.0
58-59	32.85766556452397	37.5	33.0	40.0	2.0	41.0
60-61	32.568113026354176	37.0	32.5	40.0	2.0	41.0
62-63	32.239267024457845	36.5	32.0	39.5	2.0	41.0
64-65	31.89644125923855	36.0	32.0	39.0	2.0	41.0
66-67	31.562906845643298	35.0	31.5	39.0	2.0	40.0
68-69	31.219726277594088	35.0	31.0	38.0	2.0	40.0
70-71	31.171764836380433	35.0	32.0	37.5	2.0	40.0
72-73	30.896070881552077	35.0	31.5	37.0	2.0	39.0
74-75	30.58535173344355	35.0	31.0	36.5	2.0	39.0
76-77	30.294256524725274	35.0	31.0	36.0	2.0	38.5
78-79	30.014923371705727	35.0	31.0	36.0	2.0	37.0
80-81	29.754195714407274	35.0	31.0	35.0	2.0	37.0
82-83	29.5428885964699	35.0	31.0	35.0	2.0	36.5
84-85	29.3384294770495	35.0	30.5	35.0	2.0	36.0
86-87	29.17562132281435	35.0	30.0	35.0	2.0	36.0
88-89	29.037296843090537	34.0	30.0	35.0	2.0	35.5
90-91	28.920675736044927	34.0	30.0	35.0	2.0	35.0
92-93	28.8144111488622	34.0	30.0	35.0	2.0	35.0
94-95	28.697762690848975	34.0	30.0	35.0	2.0	35.0
96-97	28.607943632694738	34.0	29.5	35.0	2.0	35.0
98-99	28.525842105902946	34.0	29.0	35.0	2.0	35.0
100-101	28.432291919916366	34.0	29.0	35.0	2.0	35.0
102-103	27.61564065083147	33.5	28.0	34.5	2.0	35.0
104-105	28.212456238451814	34.0	29.0	35.0	2.0	35.0
106-107	28.29476403712438	34.0	29.0	35.0	2.0	35.0
108-109	28.23199508594282	34.0	29.0	35.0	2.0	35.0
110-111	28.155712097636876	34.0	29.0	35.0	2.0	35.0
112-113	28.068712848268987	34.0	29.0	35.0	2.0	35.0
114-115	27.94684491393562	34.0	29.0	35.0	2.0	35.0
116-117	27.815604334824467	34.0	29.0	35.0	2.0	35.0
118-119	27.81371863755713	34.0	29.0	35.0	2.0	35.0
120-121	27.990624316225812	34.0	29.0	35.0	2.0	35.0
122-123	27.859218871559854	34.0	29.0	35.0	2.0	35.0
124-125	27.277175085699696	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75094.0
3	8890.0
4	5516.0
5	4544.0
6	4768.0
7	5284.0
8	5461.0
9	5657.0
10	6072.0
11	6541.0
12	6530.0
13	6594.0
14	6792.0
15	6087.0
16	5071.0
17	4676.0
18	4198.0
19	4094.0
20	4037.0
21	4121.0
22	4719.0
23	6090.0
24	8685.0
25	10525.0
26	11442.0
27	13380.0
28	15673.0
29	18964.0
30	22532.0
31	28543.0
32	36755.0
33	50214.0
34	77028.0
35	125778.0
36	253757.0
37	368533.0
38	83542.0
39	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.499232907981636	17.447829004208938	11.708126616155361	25.344811471654072
2	16.735507048160912	18.870090753809777	38.62135358729811	25.7730486107312
3	20.51135150496056	23.21718799982676	27.803151933657578	28.4683085615551
4	14.01900972825769	14.257296193267344	33.998448402546074	37.72524567592889
5	14.549531273031748	37.951840184542725	32.50400040117983	14.994628141245695
6	35.604546428685424	33.40269302030785	16.130839019155736	14.86192153185099
7	30.798098878094926	32.02030294856257	18.664807589290803	18.516790584051698
8	28.5348803635065	35.27228794820944	17.985441503871375	18.207390184412684
9	27.95639610643702	15.944354660399766	17.582425931962185	38.51682330120103
10-11	27.27532097004279	25.505279693646703	25.682238232997946	21.537161103312556
12-13	26.414462849633914	22.563479921847488	26.40758670495527	24.61447052356333
14-15	23.984141661341805	24.066920791123827	23.353325943108437	28.59561160442593
16-17	24.663607284773242	27.226991953926927	23.998875525198113	24.11052523610172
18-19	24.630493963829323	26.59189802978234	25.34291330236926	23.434694704019073
20-21	25.269704846139646	25.524612870309937	25.318331116026076	23.887351167524336
22-23	25.128660415899756	24.872042386992142	25.36810726063768	24.631189936470417
24-25	24.78862688009628	24.961706666990843	25.402231954266817	24.847434498646056
26-27	24.46835305027202	25.333097294839302	25.424346940116756	24.77420271477192
28-29	24.562797790226515	25.886124181611102	25.244557433672227	24.306520594490156
30-31	25.127653795233474	25.434114078292303	25.28549964574908	24.152732480725142
32-33	24.783580085150938	25.309438594185163	25.291659242774383	24.615322077889516
34-35	24.7094712928385	25.58384968107375	25.42250604991129	24.284172976176457
36-37	24.715361476678446	25.61021104849549	25.40806736046159	24.26636011436447
38-39	24.791720327412225	25.76574358787332	25.267657899355616	24.174878185358843
40-41	24.76684634633817	25.46844424048077	25.476270164576142	24.288439248604917
42-43	24.5604570942301	25.52357651921528	25.24902366009695	24.666942726457673
44-45	24.316476850305875	25.558289559511316	25.59665932322575	24.52857426695706
46-47	24.27379878239892	25.439656564313463	25.46849148534035	24.81805316794727
48-49	24.40594683799752	25.616277444139175	25.325614225971943	24.652161491891366
50-51	24.40584641053734	25.79454746314652	25.43148315019929	24.368122976116847
52-53	24.557376638148064	25.661081093196604	25.30489298971211	24.476649278943228
54-55	24.286793731572693	25.322882662064362	25.659052168386392	24.731271437976552
56-57	24.41151732453791	25.591293928680788	25.318944275642618	24.678244471138687
58-59	24.35406004206227	25.71409359462187	25.426928968258792	24.504917395057078
60-61	24.686029226567634	25.61794090484821	25.53273125606164	24.163298612522514
62-63	24.521989199620865	25.76621718905222	25.373976898661443	24.337816712665468
64-65	24.453628408815494	25.42594760758952	25.406535413388564	24.71388857020642
66-67	24.392320319228187	25.56359358124626	25.316475892824243	24.727610206701314
68-69	24.21874287694089	25.49549898643618	25.554344952087455	24.731413184535477
70-71	24.216303858097035	25.437045602875624	25.654951081435712	24.69169945759163
72-73	24.23562305781168	25.842634310157504	25.27602817265247	24.645714459378347
74-75	24.173800332326124	25.8233125179972	25.380894818857954	24.621992330818728
76-77	24.17267526231414	25.374267761763296	25.44599101939719	25.00706595652537
78-79	24.032943697124644	25.390731546097246	25.71617971637726	24.860145040400855
80-81	24.269898023531628	25.68056490496024	25.336084717509532	24.713452353998598
82-83	24.349937298728157	25.652095126623898	25.521602022244235	24.476365552403713
84-85	24.365967046280407	25.795663536376097	25.240660320480263	24.597709096863237
86-87	24.063389792518667	25.926447934019304	25.393298977884232	24.616863295577794
88-89	23.967485926004155	25.53179930989079	25.554250842502306	24.946463921602742
90-91	24.26092958815527	25.414509671480783	25.559058582007477	24.765502158356465
92-93	24.286829026637964	25.64414278539566	25.481316801012667	24.58771138695371
94-95	24.33599540475465	25.49415223117853	25.41186633975184	24.757986024314988
96-97	24.01035471430889	25.37091159552486	25.633882436609856	24.984851253556396
98-99	23.92076860832203	25.475498520299816	25.46467144577535	25.139061425602804
100-101	23.92021289730668	25.52803584759075	25.640790880822717	24.91096037427985
102-103	23.702392371762603	25.574995488778928	25.687519588196743	25.035092551261716
104-105	23.90440426465476	25.652624001124476	25.502149232525497	24.940822501695262
106-107	23.9607472772612	25.450522665556345	25.65000782592707	24.938722231255387
108-109	23.83547082821896	25.517620229101155	25.616849530465334	25.030059412214552
110-111	23.709987835557403	25.84286822512349	25.633580547833006	24.813563391486106
112-113	23.673375577437394	25.863724775103332	25.643804704595187	24.81909494286409
114-115	23.74574151570131	25.955632747706268	25.518919674577344	24.77970606201508
116-117	23.548182571390907	25.85389684516594	25.622584641310333	24.97533594213282
118-119	23.648744535150616	25.653014070596097	25.673444836361742	25.024796557891545
120-121	23.451011232113245	25.724369677663233	25.90243466929566	24.92218442092786
122-123	23.445761831698007	25.899265270956263	25.718327654736235	24.936645242609497
124-125	23.411177231467043	25.827256824082923	25.67272133277364	25.088844611676397
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	366.0
1	303.0
2	223.5
3	187.5
4	148.5
5	136.0
6	156.0
7	202.5
8	279.5
9	338.5
10	330.5
11	323.5
12	362.5
13	408.5
14	431.0
15	445.5
16	491.0
17	545.5
18	575.0
19	632.5
20	699.0
21	749.5
22	819.5
23	984.0
24	1197.0
25	1481.0
26	1918.5
27	2456.0
28	3168.0
29	4095.5
30	5229.5
31	6592.5
32	8267.5
33	10082.0
34	12060.0
35	14180.5
36	16839.0
37	19711.0
38	22314.5
39	25210.0
40	28121.0
41	30930.5
42	33761.0
43	37105.5
44	41030.5
45	44968.0
46	48768.5
47	52283.0
48	56053.5
49	59436.5
50	61300.5
51	62475.0
52	62473.0
53	61667.5
54	60679.0
55	58356.5
56	54865.0
57	50049.5
58	45165.5
59	40172.5
60	34597.5
61	29580.5
62	24352.0
63	18668.5
64	13756.5
65	10267.5
66	7703.5
67	5840.0
68	4369.5
69	3230.0
70	2493.5
71	1893.5
72	1373.0
73	1021.5
74	773.0
75	537.0
76	373.0
77	249.0
78	162.5
79	120.5
80	91.5
81	65.0
82	47.5
83	36.0
84	32.0
85	33.5
86	23.5
87	19.5
88	22.5
89	14.5
90	9.0
91	8.5
92	8.5
93	7.0
94	5.0
95	6.0
96	3.5
97	1.0
98	1.5
99	1.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0163346056598269
2	0.010332587766216084
3	0.010864412136536031
4	0.012231960517358748
5	0.00805334046484489
6	0.028718515997277056
7	0.012080010697267335
8	0.011700136147038801
9	0.007825415734707769
10-11	0.007331578819410678
12-13	0.006040005348633667
14-15	0.005394218613245162
16-17	0.004102645142468151
18-19	0.005698118253427988
20-21	0.00478641933287951
22-23	0.0049763566079937755
24-25	0.005166293883108042
26-27	0.004140632597491005
28-29	0.005394218613245162
30-31	0.005052331518039483
32-33	0.006875729359136439
34-35	0.006761766994067879
36-37	0.006153967713702227
38-39	0.006457867353885052
40-41	0.006495854808907906
42-43	0.0063818924438393465
44-45	0.006343904988816493
46-47	0.008471202470096275
48-49	0.007293591364387825
50-51	0.00501434406301663
52-53	0.0049763566079937755
54-55	0.005508180978313721
56-57	0.006457867353885052
58-59	0.006077992803656521
60-61	0.0044445322376738304
62-63	0.005052331518039483
64-65	0.0031149713118739666
66-67	0.003760758047262472
68-69	0.005774093163473695
70-71	0.004330569872605271
72-73	0.004102645142468151
74-75	0.0035708207721482053
76-77	0.004102645142468151
78-79	0.0029630214917825536
80-81	0.0028870465817368474
82-83	0.004748431877856656
84-85	0.008015353009822037
86-87	0.00619195516872508
88-89	0.004216607507536711
90-91	0.004596482057765243
92-93	0.007217616454342119
94-95	0.006153967713702227
96-97	0.008243277739959157
98-99	0.006002017893610814
100-101	0.007331578819410678
102-103	0.0046724569678109505
104-105	0.004292582417582418
106-107	0.00653384226393076
108-109	0.006115980258679374
110-111	0.007065666634250705
112-113	0.004862394242925216
114-115	0.002127297481279782
116-117	0.002849059126713994
118-119	0.15419107993776135
120-121	0.009990700671010407
122-123	0.0018613852961198094
124-125	0.00163346056598269
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1316224.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.41412138354582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.89652720448079	55.05500020032795
2	9.357537145869317	12.429452157136074
3	3.0110578142453095	5.999302775044865
4	1.4825329856541947	3.938445026573931
5	0.8421710046484	2.796602366421078
6	0.5468713618931086	2.1791988605972357
7	0.3717326396531711	1.7281807656506123
8	0.2633505269886896	1.3992155092678074
9	0.2022675727383561	1.209008081502033
>10	0.9871112841391081	11.113328443208465
>50	0.03231634744816817	1.3925681479371337
>100	0.006295203594643776	0.6628560427696817
>500	2.289086467410972E-4	0.09684162356308902
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.59749100457065E-5	0.0
5	0.0	0.0	0.0	7.59749100457065E-5	0.0
6	0.0	0.0	0.0	7.59749100457065E-5	0.0
7	0.0	0.0	0.0	7.59749100457065E-5	0.0
8	0.0	0.0	0.0	7.59749100457065E-5	0.0
9	0.0	0.0	0.0	1.51949820091413E-4	0.0
10-11	0.0	0.0	0.0	1.51949820091413E-4	0.0
12-13	0.0	3.798745502285325E-5	0.0	2.2792473013711953E-4	3.418870952056793E-4
14-15	0.0	7.59749100457065E-5	0.0	2.2792473013711953E-4	3.7987455022853254E-4
16-17	0.0	7.59749100457065E-5	0.0	2.2792473013711953E-4	3.7987455022853254E-4
18-19	0.0	7.59749100457065E-5	0.0	2.2792473013711953E-4	3.7987455022853254E-4
20-21	0.0	7.59749100457065E-5	0.0	2.659121851599728E-4	3.7987455022853254E-4
22-23	0.0	7.59749100457065E-5	0.0	4.5584946027423906E-4	3.7987455022853254E-4
24-25	0.0	7.59749100457065E-5	0.0	4.5584946027423906E-4	3.7987455022853254E-4
26-27	0.0	7.59749100457065E-5	0.0	7.977365554799183E-4	3.7987455022853254E-4
28-29	0.0	7.59749100457065E-5	0.0	0.0026211343965768744	3.7987455022853254E-4
30-31	0.0	7.59749100457065E-5	0.0	0.010408562676261791	3.7987455022853254E-4
32-33	0.0	7.59749100457065E-5	0.0	0.025033732860060297	3.7987455022853254E-4
34-35	0.0	7.59749100457065E-5	0.0	0.04338167363609841	4.5584946027423906E-4
36-37	0.0	7.59749100457065E-5	0.0	0.06921314305163863	4.5584946027423906E-4
38-39	0.0	7.59749100457065E-5	0.0	0.11297469123796558	4.5584946027423906E-4
40-41	0.0	7.59749100457065E-5	0.0	0.17204518379850237	4.5584946027423906E-4
42-43	0.0	7.59749100457065E-5	0.0	0.22104900077798306	4.5584946027423906E-4
44-45	0.0	7.59749100457065E-5	0.0	0.2717622532334922	4.5584946027423906E-4
46-47	0.0	7.59749100457065E-5	0.0	0.32885739813284065	4.5584946027423906E-4
48-49	0.0	7.59749100457065E-5	0.0	0.3914227365554799	5.318243703199456E-4
50-51	0.0	7.59749100457065E-5	0.0	0.4620414154429641	5.318243703199456E-4
52-53	0.0	7.59749100457065E-5	0.0	0.5351672663619567	5.698118253427988E-4
54-55	0.0	7.59749100457065E-5	0.0	0.616346457745794	6.07799280365652E-4
56-57	0.0	7.59749100457065E-5	0.0	0.6948285398230089	6.457867353885053E-4
58-59	0.0	7.59749100457065E-5	0.0	0.7846308834970339	6.837741904113585E-4
60-61	0.0	7.59749100457065E-5	0.0	0.8845378902071379	6.837741904113585E-4
62-63	0.0	7.59749100457065E-5	0.0	0.9979684309053778	6.837741904113585E-4
64-65	0.0	7.59749100457065E-5	0.0	1.1018261329378585	6.837741904113585E-4
66-67	0.0	7.59749100457065E-5	0.0	1.215636548186327	6.837741904113585E-4
68-69	0.0	1.51949820091413E-4	0.0	1.345363707089371	6.837741904113585E-4
70-71	0.0	1.51949820091413E-4	0.0	1.4787376616746086	6.837741904113585E-4
72-73	0.0	1.51949820091413E-4	0.0	1.609148594768064	7.597491004570651E-4
74-75	0.0	1.51949820091413E-4	0.0	1.7449157590197413	7.597491004570651E-4
76-77	0.0	1.51949820091413E-4	0.0	1.9063624428668677	7.597491004570651E-4
78-79	0.0	1.51949820091413E-4	0.0	2.083953795098707	7.597491004570651E-4
80-81	0.0	1.51949820091413E-4	0.0	2.25960778712438	7.597491004570651E-4
82-83	0.0	1.51949820091413E-4	0.0	2.4382248006418363	7.597491004570651E-4
84-85	0.0	1.51949820091413E-4	0.0	2.621970120587377	7.597491004570651E-4
86-87	0.0	1.51949820091413E-4	0.0	2.837093078381795	8.357240105027715E-4
88-89	0.0	1.51949820091413E-4	0.0	3.0856070091413015	9.116989205484781E-4
90-91	0.0	1.51949820091413E-4	0.0	3.345099314402412	9.116989205484781E-4
92-93	0.0	1.51949820091413E-4	0.0	3.5951707308178547	9.876738305941845E-4
94-95	0.0	1.51949820091413E-4	0.0	3.8510922153068172	9.876738305941845E-4
96-97	0.0	3.03899640182826E-4	0.0	4.141316371681416	0.0011396236506855976
98-99	0.0	3.03899640182826E-4	0.0	4.460259044053291	0.0011396236506855976
100-101	0.0	3.418870952056793E-4	0.0	4.78907845473111	0.0011396236506855976
102-103	0.0	3.7987455022853254E-4	0.0	5.13138341194204	0.001215598560731304
104-105	0.0	3.7987455022853254E-4	0.0	5.4922262472041234	0.0012915734707770105
106-107	0.0	3.7987455022853254E-4	0.0	5.875177781289507	0.0013295609257998639
108-109	0.0	3.7987455022853254E-4	0.0	6.277199017796363	0.001367548380822717
110-111	0.0	3.7987455022853254E-4	0.0	6.727046460176991	0.0014055358358455703
112-113	0.0	3.7987455022853254E-4	0.0	7.175108492171545	0.0014435232908684237
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2190	0.0	52.717106	1
GGTATCA	1305	0.0	50.162144	1
TCAACGC	2490	0.0	45.167225	4
CAACGCA	2635	0.0	42.455914	5
TATCAAC	2755	0.0	41.038635	2
AACGCAG	2810	0.0	40.23233	6
ATCAACG	2850	0.0	39.253098	3
GTACATG	6080	0.0	38.662277	1
TACATGG	6335	0.0	36.633545	2
ACATGGG	6435	0.0	35.13953	3
CATGGGG	5780	0.0	33.150414	4
ACGCAGA	3630	0.0	31.304384	7
ATGGGGG	3690	0.0	31.123745	5
CGCAGAG	3790	0.0	29.981688	8
TACCGTA	85	0.007213869	27.997513	7
TATACTG	475	0.0	27.560684	5
GAGTACA	4385	0.0	25.64993	1
GCAGAGT	4380	0.0	25.126183	9
AGTACAT	4350	0.0	25.033567	2
TGGGGGG	2735	0.0	24.583801	6
>>END_MODULE