FastQCFastQC Report
Mon 15 Aug 2016
SRR2921313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316224
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18610.1413893075950598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.1166974618302052No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15650.061.4833341
GTATCAA25800.053.742041
GTACATG59450.038.2012521
TATCAAC37600.036.29962
TACATGG62000.036.2413022
TCAACGC36550.036.0378654
CAACGCA37850.034.4851725
ACATGGG65200.034.096943
ATCAACG39800.033.544333
CATGGGG58250.033.458464
AACGCAG40200.032.159266
ATGGGGG38150.031.7143385
TGGGGGG28000.027.447586
ACGCAGA49650.025.9104357
GTGGTAT8750.025.2719961
CGCAGAG51800.024.6013078
AGTACAT41050.022.7950552
GAGTACA42350.022.7205221
AGAGTAC52200.022.5296410-11
ACCGTAT1602.5970492E-422.3308588
Produced by FastQC (version 0.11.5)