FastQCFastQC Report
Mon 15 Aug 2016
SRR2921312_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921312_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524120
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19900.050.5381241
GGTATCA12850.048.6263471
TCAACGC23400.040.6901784
CAACGCA24000.039.6716235
AACGCAG25750.036.9730646
GTACATG67300.034.6622281
TATCAAC28250.033.915082
ATCAACG28800.033.474033
CGTATAC1102.0380043E-532.4596633
TACATGG70700.031.9010722
ACATGGG72100.030.6213363
TATCTCG1903.715104E-828.1877335
CATGGGG68500.028.1475074
GAGTACA48100.027.7133121
ACGCAGA34000.027.471227
AGTACAT47350.027.1468372
CGCAGAG36150.026.0019558
TATACCG1701.3977984E-524.5030615
ATGGGGG39050.023.467725
GTATCGC1300.002051757622.8859716
Produced by FastQC (version 0.11.5)