##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921312_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1524120
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.01251607484975	31.0	31.0	34.0	26.0	34.0
2	29.96836535180957	31.0	31.0	34.0	26.0	34.0
3	30.189279715507965	31.0	31.0	34.0	26.0	34.0
4	33.682749389811825	37.0	35.0	37.0	28.0	37.0
5	33.69203081122221	37.0	35.0	37.0	28.0	37.0
6	33.69076713119702	37.0	35.0	37.0	29.0	37.0
7	33.69738012754901	37.0	35.0	37.0	29.0	37.0
8	33.67174303860588	37.0	35.0	37.0	28.0	37.0
9	35.25672650447471	39.0	37.0	39.0	28.0	39.0
10-11	35.26380271894601	39.0	37.0	39.0	28.5	39.0
12-13	35.178264834789914	39.0	37.0	39.0	28.0	39.0
14-15	36.43534728236622	40.0	38.0	41.0	27.0	41.0
16-17	36.407593562186705	40.0	38.0	41.0	27.0	41.0
18-19	36.37535397475264	40.0	37.5	41.0	27.0	41.0
20-21	36.409100661365244	40.0	38.0	41.0	27.0	41.0
22-23	36.347615673306564	40.0	37.5	41.0	27.0	41.0
24-25	36.32279610529355	40.0	37.0	41.0	27.0	41.0
26-27	36.28810428312731	40.0	37.0	41.0	27.0	41.0
28-29	36.22266127339054	40.0	37.0	41.0	27.0	41.0
30-31	36.14556432564365	40.0	37.0	41.0	27.0	41.0
32-33	36.07293454583628	40.0	37.0	41.0	27.0	41.0
34-35	35.948476497913546	40.0	37.0	41.0	26.5	41.0
36-37	35.835541164737684	40.0	36.5	41.0	26.0	41.0
38-39	35.73083484240087	40.0	36.0	41.0	25.5	41.0
40-41	35.62678201191507	40.0	36.0	41.0	25.0	41.0
42-43	35.41349992126604	40.0	35.5	41.0	24.5	41.0
44-45	35.10673864262657	40.0	35.0	41.0	24.0	41.0
46-47	34.86986523370863	39.0	35.0	41.0	23.0	41.0
48-49	34.64938915570953	39.0	35.0	41.0	22.5	41.0
50-51	34.46853594205181	39.0	34.5	40.5	22.0	41.0
52-53	34.260076962443904	38.5	34.0	40.0	21.0	41.0
54-55	34.048927905939166	38.0	34.0	40.0	20.0	41.0
56-57	33.792753523344615	38.0	34.0	40.0	18.5	41.0
58-59	33.50400821457628	37.5	33.0	40.0	18.0	41.0
60-61	33.21771710888906	37.0	33.0	40.0	16.0	41.0
62-63	32.900523580820405	36.5	33.0	39.5	13.5	41.0
64-65	32.54825407448233	36.0	32.5	39.0	12.0	41.0
66-67	32.21236877673674	35.5	32.0	39.0	9.0	40.0
68-69	31.864925333963207	35.0	32.0	38.5	8.0	40.0
70-71	31.836817967089203	35.0	32.0	38.0	7.5	40.0
72-73	31.560464727186837	35.0	32.0	37.0	7.0	39.0
74-75	31.242266028921605	35.0	32.0	37.0	4.0	39.0
76-77	30.950665301944728	35.0	32.0	36.0	2.0	39.0
78-79	30.67071818491982	35.0	31.5	36.0	2.0	37.5
80-81	30.395345182794006	35.0	31.0	35.0	2.0	37.0
82-83	30.177741253969504	35.0	31.0	35.0	2.0	36.5
84-85	29.962082710022834	35.0	31.0	35.0	2.0	36.0
86-87	29.795580728551556	35.0	31.0	35.0	2.0	36.0
88-89	29.6586800908065	34.5	31.0	35.0	2.0	35.5
90-91	29.53276152796368	34.0	31.0	35.0	2.0	35.0
92-93	29.420608613495	34.0	31.0	35.0	2.0	35.0
94-95	29.300829002965646	34.0	30.0	35.0	2.0	35.0
96-97	29.2138945752303	34.0	30.0	35.0	2.0	35.0
98-99	29.13384772852531	34.0	30.0	35.0	2.0	35.0
100-101	29.03417184998557	34.0	30.0	35.0	2.0	35.0
102-103	28.156262958297248	33.5	28.0	34.5	2.0	35.0
104-105	28.78906647770517	34.0	29.5	35.0	2.0	35.0
106-107	28.89114800671863	34.0	30.0	35.0	2.0	35.0
108-109	28.838257158229013	34.0	30.0	35.0	2.0	35.0
110-111	28.761365574889115	34.0	30.0	35.0	2.0	35.0
112-113	28.667010799674564	34.0	30.0	35.0	2.0	35.0
114-115	28.54174277615936	34.0	29.0	35.0	2.0	35.0
116-117	28.411217948717947	34.0	29.0	35.0	2.0	35.0
118-119	28.41313512059418	34.0	29.5	35.0	2.0	35.0
120-121	28.60035987979949	34.0	30.0	35.0	2.0	35.0
122-123	28.46462253628323	34.0	30.0	35.0	2.0	35.0
124-125	27.8489817074771	34.0	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65483.0
3	8954.0
4	5738.0
5	4826.0
6	4950.0
7	5631.0
8	5698.0
9	6232.0
10	6642.0
11	6934.0
12	6823.0
13	6612.0
14	6598.0
15	6133.0
16	5417.0
17	5125.0
18	4833.0
19	4730.0
20	4650.0
21	4704.0
22	5459.0
23	6966.0
24	9223.0
25	11694.0
26	13102.0
27	15453.0
28	18419.0
29	21962.0
30	27020.0
31	33741.0
32	44225.0
33	60134.0
34	92017.0
35	151405.0
36	304166.0
37	434766.0
38	97603.0
39	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.91431383807727	17.432269707161023	11.839947502255763	25.813468952505946
2	16.584971180194653	19.14161338414858	38.00008136739215	26.27333406826462
3	20.4123467807554	22.294876505945037	27.97967138245098	29.313105330848575
4	13.827984069998564	14.135415511474706	34.08551778082402	37.95108263770271
5	14.76718633426663	37.35609293255126	32.97980888288417	14.896911850297936
6	35.77788672503877	33.23304934851507	15.85865208611034	15.13041184033582
7	30.88498548472615	31.121350839156065	19.02970775215367	18.96395592396411
8	27.903433251089293	35.22743976061736	18.202727177279648	18.6663998110137
9	27.311083449858366	15.724087971532677	17.68964818079873	39.275180397810225
10-11	26.944411180427853	25.239361483987068	26.056715786227386	21.759511549357693
12-13	26.415539413321927	22.362906297581763	26.52551161515766	24.696042673938656
14-15	23.729160542560788	23.956649204216184	23.363879498593203	28.950310754629825
16-17	24.50466026488545	27.23133351486659	24.07377030356516	24.1902359166828
18-19	24.635226757496085	26.511634621125975	25.434227301448985	23.418911319928952
20-21	25.061735483098403	25.423962914420038	25.36264572674318	24.151655875738374
22-23	25.0661316859602	24.91761220569636	25.29119102649696	24.72506508184647
24-25	24.630890472326996	25.36869943331521	25.273295081918807	24.727115012438983
26-27	24.54164264907502	25.576413997775017	25.382851620002278	24.499091733147687
28-29	24.527479838888265	25.8707556352854	25.34169971762469	24.260064808201644
30-31	24.89094788726295	25.552543827532315	25.19474528917182	24.36176299603292
32-33	24.503447488464627	25.475752095789524	25.348685833670164	24.672114582075686
34-35	24.51185571762418	25.70600681162839	25.510338996785787	24.27179847396165
36-37	24.537020784893592	25.793438452561666	25.339702909845734	24.329837852699004
38-39	24.648626197328376	25.793133676898826	25.43870872916199	24.119531396610803
40-41	24.576316228066148	25.669449230781343	25.36259509427079	24.391639446881722
42-43	24.4087156736852	25.5540143030276	25.454441661586873	24.582828361700326
44-45	24.323571512231997	25.660368939817214	25.533434535278676	24.482625012672116
46-47	24.42048162017768	25.666461720979463	25.391261439611124	24.521795219231734
48-49	24.207948614223803	25.788721336560368	25.524449943126697	24.478880106089136
50-51	24.284080936328667	25.71783830778442	25.52916184805164	24.468918907835274
52-53	24.383999286110082	25.756006468315068	25.531406727297007	24.328587518277843
54-55	24.171243348228053	25.479386888709538	25.55582895349829	24.793540809564117
56-57	24.349241688295457	25.60395745304904	25.407698962703996	24.639101895951505
58-59	24.350262152329517	25.81641889517745	25.518161360902113	24.315157591590918
60-61	24.578663619521546	25.55097455824105	25.50934205221025	24.361019770027152
62-63	24.42767175096684	25.61360018582214	25.596474628585376	24.362253434625647
64-65	24.31255730944558	25.38835914958114	25.685949979413707	24.61313356155957
66-67	24.276187604797826	25.894753211066057	25.334869137842176	24.494190046293944
68-69	24.258370835415263	25.604640076796564	25.52760729554243	24.60938179224574
70-71	24.195156998927203	25.497964312311566	25.6719737804738	24.63490490828743
72-73	24.347908247526178	25.748971331124753	25.381104512495266	24.522015908853803
74-75	24.088105137213592	25.716151963285043	25.595192595068912	24.600550304432453
76-77	24.098835015796674	25.472866792865084	25.623189451824246	24.805108739513994
78-79	24.101676131973864	25.46735740280413	25.681913843450566	24.74905262177144
80-81	24.170570401906204	25.780957558177107	25.412930929557575	24.635541110359107
82-83	24.137312218471237	25.802190880190544	25.484778992746275	24.575717908591937
84-85	24.314714899941436	25.727854486518055	25.397306071427984	24.560124542112526
86-87	24.00093962052323	25.825620997631592	25.569325064410265	24.604114317434913
88-89	24.118986558551548	25.53675081829423	25.57405263394452	24.770209989209704
90-91	24.196571430071195	25.434689820815837	25.62628502476795	24.742453724345026
92-93	24.208513260179306	25.747785923744043	25.583054844524177	24.460645971552474
94-95	24.198065837127306	25.4895199762995	25.609006714813503	24.703407471759693
96-97	23.935639511974742	25.5420270036618	25.687107790562663	24.835225693800798
98-99	24.041671355470612	25.551657605655564	25.60785763708559	24.79881340178823
100-101	23.901562636873035	25.547172250293553	25.729423564192654	24.821841548640762
102-103	23.810342434900885	25.579684017790854	25.790472241077424	24.819501306230833
104-105	23.86539304433182	25.596928451260194	25.75427216394191	24.783406340466072
106-107	23.76971973655072	25.514849779775428	25.86281280501308	24.85261767866077
108-109	23.64494799605517	25.519530924483224	25.93333810142787	24.90218297803374
110-111	23.66301291662429	25.79023690867156	25.74722523876234	24.79952493594181
112-113	23.64737558860573	25.838220815440998	25.728283224954097	24.78612037099918
114-115	23.689961166902272	25.815562305682782	25.751031847601823	24.74344467981312
116-117	23.46981357265601	25.601363183929443	25.97394885698182	24.954874386432724
118-119	23.625410442329752	25.586826152516146	25.8383242485369	24.949439156617203
120-121	23.377351575821145	25.762368421829695	26.02139897496738	24.838881027381777
122-123	23.438545704590013	25.776593538972715	25.913954013274786	24.870906743162486
124-125	23.387003382003698	25.66494313188174	25.906233627595103	25.04181985851946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	322.0
1	262.0
2	178.5
3	133.5
4	109.0
5	105.0
6	106.0
7	119.5
8	128.0
9	156.0
10	175.0
11	180.5
12	197.5
13	202.5
14	230.0
15	270.0
16	301.0
17	333.0
18	379.5
19	436.5
20	494.5
21	551.5
22	667.0
23	891.5
24	1130.5
25	1487.0
26	2021.0
27	2685.5
28	3544.5
29	4608.5
30	5878.0
31	7437.0
32	9430.0
33	11707.5
34	14227.0
35	16987.5
36	20552.0
37	24072.5
38	26949.5
39	30128.0
40	33648.5
41	36604.0
42	39648.0
43	44698.0
44	50206.5
45	55742.5
46	59336.5
47	61874.0
48	66286.5
49	68982.5
50	69576.5
51	70485.5
52	70442.0
53	69302.0
54	69123.0
55	68025.0
56	63979.5
57	58116.0
58	53296.5
59	48883.5
60	41576.5
61	33461.5
62	26851.5
63	20620.0
64	14852.5
65	10776.5
66	7843.0
67	5613.0
68	4161.0
69	2992.0
70	2170.5
71	1621.0
72	1155.0
73	809.5
74	573.5
75	368.0
76	244.5
77	156.5
78	99.0
79	77.5
80	53.5
81	36.5
82	26.0
83	19.5
84	19.5
85	12.0
86	6.5
87	10.5
88	9.5
89	5.0
90	2.0
91	1.0
92	5.0
93	4.5
94	1.0
95	1.5
96	1.5
97	1.5
98	2.0
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016074849749363566
2	0.011022754113849304
3	0.01049786106080886
4	0.013319161220901241
5	0.008726347006797365
6	0.029328399338634754
7	0.013647219379051518
8	0.013122326326011076
9	0.008332677217017033
10-11	0.0077093667165315065
12-13	0.0064627457155604535
14-15	0.005773823583444873
16-17	0.0042647560559536
18-19	0.005970658478335039
20-21	0.005084901451329292
22-23	0.005084901451329292
24-25	0.005281736346219458
26-27	0.0041663386085085165
28-29	0.005544182872739679
30-31	0.005117707267144319
32-33	0.007348502742566202
34-35	0.007118862031861009
36-37	0.006856415505340788
38-39	0.0066923864262656485
40-41	0.00695483295278587
42-43	0.007020444584415924
44-45	0.006856415505340788
46-47	0.009120016796577698
48-49	0.007774978348161562
50-51	0.0054129596094795685
52-53	0.004953678188069181
54-55	0.005905046846704984
56-57	0.0071844736634910636
58-59	0.006659580610450621
60-61	0.004625620029918904
62-63	0.005380153793664541
64-65	0.00311655250242763
66-67	0.003575833923838018
68-69	0.006134687557410178
70-71	0.0042647560559536
72-73	0.004133532792693489
74-75	0.003575833923838018
76-77	0.0042647560559536
78-79	0.0029853292391675197
80-81	0.0029853292391675197
82-83	0.0049208723722541535
84-85	0.009021599349132613
86-87	0.006593968978820565
88-89	0.004297561871768627
90-91	0.00482245492480907
92-93	0.0079390074272367
94-95	0.006659580610450621
96-97	0.009284045875652836
98-99	0.00652835734719051
100-101	0.00823425976957195
102-103	0.0051505130829593475
104-105	0.004461590950843766
106-107	0.007053250400230953
108-109	0.006626774794635593
110-111	0.007545337637456369
112-113	0.00482245492480907
114-115	0.0021979896596068553
116-117	0.0026900768968322704
118-119	0.15999396372989003
120-121	0.011055559929664331
122-123	0.0018371256856415505
124-125	0.0015418733433063014
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1524120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.23238807741043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.05634402178264	49.020411252004706
2	10.939290839327109	13.396778039306767
3	3.593895559242017	6.601884227795675
4	1.6716221729803638	4.094296704589509
5	0.9519313716989399	2.9144515587465563
6	0.5987077759864584	2.1996184130499676
7	0.42194188318451975	1.8085556396087523
8	0.31143808975188775	1.5256078379019977
9	0.23032022010562964	1.2692751389624253
>10	1.1487371663984949	12.17397862542248
>50	0.051996507392568184	2.167955297106041
>100	0.022703539202203523	2.3608471439274825
>500	0.0010708529471946447	0.46634012157771204
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.561163163005538E-5
2	0.0	0.0	0.0	0.0	6.561163163005538E-5
3	0.0	0.0	0.0	0.0	6.561163163005538E-5
4	0.0	0.0	0.0	0.0	1.3122326326011076E-4
5	0.0	0.0	0.0	0.0	1.3122326326011076E-4
6	0.0	0.0	0.0	0.0	1.3122326326011076E-4
7	0.0	0.0	0.0	0.0	1.3122326326011076E-4
8	0.0	0.0	0.0	0.0	1.3122326326011076E-4
9	0.0	0.0	0.0	0.0	2.624465265202215E-4
10-11	0.0	0.0	0.0	0.0	2.624465265202215E-4
12-13	0.0	0.0	0.0	6.561163163005538E-5	2.624465265202215E-4
14-15	0.0	0.0	0.0	6.561163163005538E-5	3.6086397396530457E-4
16-17	0.0	0.0	0.0	9.841744744508308E-5	4.592814214103876E-4
18-19	0.0	0.0	0.0	1.3122326326011076E-4	5.905046846704984E-4
20-21	0.0	0.0	0.0	1.9683489489016615E-4	5.905046846704984E-4
22-23	0.0	0.0	0.0	3.6086397396530457E-4	7.217279479306091E-4
24-25	0.0	0.0	0.0	4.920872372254153E-4	8.857570270057475E-4
26-27	0.0	0.0	0.0	0.0010825919218959137	9.185628428207752E-4
28-29	0.0	0.0	0.0	0.003378999028947852	9.51368658635803E-4
30-31	0.0	0.0	0.0	0.01322074377345616	0.0010169802902658585
32-33	0.0	0.0	0.0	0.027819331811143478	0.0011810093693409968
34-35	6.561163163005538E-5	0.0	0.0	0.044812744403327824	0.0013122326326011075
36-37	6.561163163005538E-5	0.0	0.0	0.06925307718552345	0.0013122326326011075
38-39	6.561163163005538E-5	0.0	0.0	0.11235991916646984	0.0013122326326011075
40-41	6.561163163005538E-5	0.0	0.0	0.16065008004619058	0.0013122326326011075
42-43	6.561163163005538E-5	0.0	0.0	0.20762800829331024	0.0013122326326011075
44-45	6.561163163005538E-5	0.0	0.0	0.2593299740177939	0.0013122326326011075
46-47	6.561163163005538E-5	0.0	0.0	0.31614964700942183	0.0013122326326011075
48-49	6.561163163005538E-5	0.0	0.0	0.37670918300396294	0.0014434558958612183
50-51	6.561163163005538E-5	0.0	0.0	0.44199275647586805	0.0014434558958612183
52-53	6.561163163005538E-5	0.0	0.0	0.5037661076555652	0.001574679159121329
54-55	6.561163163005538E-5	0.0	0.0	0.571050835892187	0.0017059024223814397
56-57	6.561163163005538E-5	0.0	0.0	0.6505721334278141	0.001771514054011495
58-59	6.561163163005538E-5	0.0	0.0	0.7313400519644122	0.001869931501456578
60-61	6.561163163005538E-5	0.0	0.0	0.8206374826129177	0.001902737317271606
62-63	6.561163163005538E-5	0.0	0.0	0.9117392331312495	0.0020339605805317165
64-65	6.561163163005538E-5	0.0	0.0	1.0031034301761017	0.0020667663963467443
66-67	6.561163163005538E-5	0.0	0.0	1.1164803296328372	0.002099572212161772
68-69	6.561163163005538E-5	0.0	0.0	1.2373697609112144	0.002099572212161772
70-71	6.561163163005538E-5	0.0	0.0	1.3719064115686428	0.0022307954754218827
72-73	6.561163163005538E-5	0.0	0.0	1.4972246279820487	0.0023620187386819936
74-75	6.561163163005538E-5	0.0	0.0	1.631531637928772	0.0023620187386819936
76-77	6.561163163005538E-5	0.0	0.0	1.7806012649922578	0.0023948245544970215
78-79	6.561163163005538E-5	0.0	0.0	1.9550297876807599	0.002427630370312049
80-81	6.561163163005538E-5	0.0	0.0	2.1367083956643835	0.0024604361861270763
82-83	6.561163163005538E-5	0.0	0.0	2.324718526100307	0.00255885363357216
84-85	6.561163163005538E-5	0.0	0.0	2.5262118468362074	0.00255885363357216
86-87	6.561163163005538E-5	0.0	0.0	2.7353489226570087	0.00255885363357216
88-89	6.561163163005538E-5	0.0	0.0	2.971747631420098	0.002624465265202215
90-91	6.561163163005538E-5	0.0	0.0	3.2289780332257303	0.002624465265202215
92-93	6.561163163005538E-5	0.0	0.0	3.4907684434296513	0.0026572710810172425
94-95	6.561163163005538E-5	0.0	0.0	3.762695850720416	0.0027556885284623256
96-97	6.561163163005538E-5	0.0	0.0	4.046794215678555	0.0027556885284623256
98-99	6.561163163005538E-5	0.0	0.0	4.364780988373619	0.0027556885284623256
100-101	6.561163163005538E-5	0.0	0.0	4.71498307219904	0.0027556885284623256
102-103	6.561163163005538E-5	0.0	0.0	5.08880534341128	0.0027884943442773535
104-105	6.561163163005538E-5	0.0	0.0	5.468532661470226	0.0028541059759074087
106-107	6.561163163005538E-5	0.0	0.0	5.868533973702858	0.0029197176075374644
108-109	6.561163163005538E-5	0.0	0.0	6.287726688187282	0.0030837466866126028
110-111	6.561163163005538E-5	0.0	0.0	6.746056740939034	0.0033789990289478516
112-113	6.561163163005538E-5	0.0	0.0	7.220527255071779	0.0034774164763929348
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1990	0.0	50.538124	1
GGTATCA	1285	0.0	48.626347	1
TCAACGC	2340	0.0	40.690178	4
CAACGCA	2400	0.0	39.671623	5
AACGCAG	2575	0.0	36.973064	6
GTACATG	6730	0.0	34.662228	1
TATCAAC	2825	0.0	33.91508	2
ATCAACG	2880	0.0	33.47403	3
CGTATAC	110	2.0380043E-5	32.459663	3
TACATGG	7070	0.0	31.901072	2
ACATGGG	7210	0.0	30.621336	3
TATCTCG	190	3.715104E-8	28.187733	5
CATGGGG	6850	0.0	28.147507	4
GAGTACA	4810	0.0	27.713312	1
ACGCAGA	3400	0.0	27.47122	7
AGTACAT	4735	0.0	27.146837	2
CGCAGAG	3615	0.0	26.001955	8
TATACCG	170	1.3977984E-5	24.503061	5
ATGGGGG	3905	0.0	23.46772	5
GTATCGC	130	0.0020517576	22.885971	6
>>END_MODULE