FastQCFastQC Report
Mon 15 Aug 2016
SRR2921312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524120
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16890.11081804582316351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.063.4562571
GTATCAA26950.048.5675741
GTACATG66350.036.842021
TACATGG69950.035.021012
ACATGGG69500.034.8189623
TCAACGC35700.034.0593644
TATCAAC39900.032.7149162
CAACGCA37800.032.0094955
ATCAACG40100.031.2140053
CATGGGG67000.030.9584354
AACGCAG39250.030.5202546
GTGGTAT9600.028.6383251
ATGGGGG38000.028.233425
TGGTATC10500.024.9768662
ACGCAGA50950.023.2731427
GTATAGG3950.022.6963061
CGCAGAG53150.022.3054168
AGAGTAC52200.022.1516365
CAGAGTA58700.020.7141274
AGTACAT45200.020.5651687
Produced by FastQC (version 0.11.5)