##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921312_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1524120
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.382263207621445	33.0	30.0	34.0	22.0	34.0
2	30.15440057213343	33.0	31.0	34.0	26.0	34.0
3	30.396501587801485	33.0	31.0	34.0	27.0	34.0
4	32.575025588536334	37.0	35.0	37.0	19.0	37.0
5	32.2478223499462	37.0	35.0	37.0	19.0	37.0
6	32.41012649922578	37.0	35.0	37.0	17.0	37.0
7	32.62516074849749	37.0	35.0	37.0	17.0	37.0
8	32.87730428050285	37.0	35.0	37.0	17.0	37.0
9	34.63370338293573	39.0	37.0	39.0	22.0	39.0
10-11	34.829740112327116	39.0	37.0	39.0	26.0	39.0
12-13	34.87066077474215	39.0	37.0	39.0	27.0	39.0
14-15	36.303514158990104	41.0	38.0	41.0	27.0	41.0
16-17	36.33198927905939	41.0	38.0	41.0	27.0	41.0
18-19	36.343984397553996	41.0	38.0	41.0	27.0	41.0
20-21	36.33678286486629	41.0	38.0	41.0	27.0	41.0
22-23	36.300183384510404	41.0	38.0	41.0	27.0	41.0
24-25	36.253540731700916	41.0	38.0	41.0	27.0	41.0
26-27	36.17076608141092	41.0	38.0	41.0	27.0	41.0
28-29	36.10103666377975	41.0	38.0	41.0	26.0	41.0
30-31	36.01278836312102	40.0	38.0	41.0	25.5	41.0
32-33	35.89913983150933	40.0	37.5	41.0	25.0	41.0
34-35	35.821419901320105	40.0	37.0	41.0	25.0	41.0
36-37	35.751594034590454	40.0	37.0	41.0	24.0	41.0
38-39	35.656300356927275	40.0	37.0	41.0	24.0	41.0
40-41	35.53023449597145	40.0	37.0	41.0	23.0	41.0
42-43	35.394973492900824	40.0	36.0	41.0	21.5	41.0
44-45	35.25125843109466	40.0	36.0	41.0	20.0	41.0
46-47	35.05251817442196	40.0	35.5	41.0	16.5	41.0
48-49	34.852017557672625	40.0	35.0	41.0	13.5	41.0
50-51	34.677092026874526	40.0	35.0	41.0	10.0	41.0
52-53	34.48766665354434	39.5	35.0	41.0	8.5	41.0
54-55	34.2932702149437	39.0	35.0	41.0	7.5	41.0
56-57	34.05198672020576	39.0	35.0	41.0	6.5	41.0
58-59	33.984445778547624	39.0	35.0	41.0	2.0	41.0
60-61	33.739834789911555	38.0	35.0	41.0	2.0	41.0
62-63	33.45683213920164	37.0	34.5	40.5	2.0	41.0
64-65	33.15015910820671	37.0	34.0	40.0	2.0	41.0
66-67	32.82914501482823	36.0	34.0	39.5	2.0	41.0
68-69	32.49383709944099	36.0	34.0	39.0	2.0	41.0
70-71	32.15526992625253	35.0	34.0	39.0	2.0	40.5
72-73	31.80508326116054	35.0	34.0	37.0	2.0	39.5
74-75	31.47262846757473	35.0	33.0	37.0	2.0	39.0
76-77	31.17428155263365	35.0	33.0	36.5	2.0	39.0
78-79	30.915925911345564	35.0	33.0	36.0	2.0	37.5
80-81	30.68673037556098	35.0	33.0	36.0	2.0	37.0
82-83	30.486995774610925	35.0	33.0	35.0	2.0	37.0
84-85	30.31191999317639	35.0	33.0	35.0	2.0	36.0
86-87	30.167878841561034	35.0	33.0	35.0	2.0	36.0
88-89	30.048165498779625	35.0	33.0	35.0	2.0	36.0
90-91	29.947824974411464	35.0	32.0	35.0	2.0	35.5
92-93	29.884808610870536	35.0	32.0	35.0	2.0	35.0
94-95	29.81539084848962	35.0	32.0	35.0	2.0	35.0
96-97	29.753033553788413	35.0	32.0	35.0	2.0	35.0
98-99	29.695542017688894	35.0	32.0	35.0	2.0	35.0
100-101	29.64479666955358	35.0	32.0	35.0	2.0	35.0
102-103	28.981426987376324	34.0	30.5	35.0	2.0	35.0
104-105	29.520563341469177	35.0	32.0	35.0	2.0	35.0
106-107	29.58173831456841	35.0	32.0	35.0	2.0	35.0
108-109	29.651657677873132	35.0	32.5	35.0	2.0	35.0
110-111	29.75641156864289	35.0	33.0	35.0	2.0	35.0
112-113	29.721460908589876	35.0	33.0	35.0	2.0	35.0
114-115	29.66407467915912	35.0	33.0	35.0	2.0	35.0
116-117	29.608379261475473	35.0	33.0	35.0	2.0	35.0
118-119	29.537138479909718	35.0	33.0	35.0	2.0	35.0
120-121	29.449733616775582	35.0	33.0	35.0	2.0	35.0
122-123	29.345476734115422	35.0	32.5	35.0	2.0	35.0
124-125	28.837442589822324	34.5	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96905.0
3	5217.0
4	4029.0
5	3811.0
6	4011.0
7	4299.0
8	4665.0
9	4657.0
10	4807.0
11	4922.0
12	5355.0
13	6611.0
14	7804.0
15	6790.0
16	4375.0
17	3871.0
18	3468.0
19	3253.0
20	3179.0
21	3237.0
22	3734.0
23	4712.0
24	6083.0
25	7830.0
26	9383.0
27	10946.0
28	13342.0
29	15768.0
30	19021.0
31	23823.0
32	30487.0
33	41404.0
34	61450.0
35	105477.0
36	246617.0
37	542313.0
38	196083.0
39	381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71637948630159	17.60467214032223	11.477491950829279	27.201456422546894
2	16.399561714300713	19.195732619478783	37.04071857858961	27.363987087630896
3	19.79975330026507	21.934165288822403	28.017544550297877	30.24853686061465
4	14.09241034545439	15.062633007782125	30.94232671009537	39.90262993666811
5	14.697909998067612	37.16506927017537	32.69402419033622	15.442996541420792
6	35.79914730488609	33.57189979027245	15.268034436016686	15.360918468824773
7	31.18279358191628	31.153655077548454	18.046972056568077	19.616579283967194
8	28.431804208303614	35.34137758392952	17.214845008520413	19.01197319924645
9	27.58599647247798	15.861775729388668	17.333479438536077	39.21874835959727
10-11	27.39528963279607	25.337628806792274	24.989452688006246	22.277628872405412
12-13	26.903712593248123	22.476974779102967	26.068640072583033	24.550672555065876
14-15	24.150919875075452	23.885553630947694	23.18695378316668	28.77657271081017
16-17	24.527366611552896	27.19966275621342	23.85504422223972	24.417926409993964
18-19	24.58727003123114	26.162900559011103	25.645552843608115	23.604276566149647
20-21	25.078340288166284	24.920741148990892	25.814765241582027	24.186153321260793
22-23	25.002690076896833	24.501089153085058	25.712771960213104	24.783448809805
24-25	24.54632837309398	25.23413510747185	25.60064168175734	24.618894837676823
26-27	24.43337794924284	25.27294438758103	25.801478886176945	24.492198776999185
28-29	24.442924441645015	25.517971025903474	25.70913051465764	24.329974017793873
30-31	24.727088418234786	25.296039681914813	25.611697241687004	24.3651746581634
32-33	24.296052804241135	25.381695667007847	25.735998477810146	24.586253050940872
34-35	24.484883080072436	25.395802167808306	25.92400860824607	24.195306143873186
36-37	24.621781749468546	25.499271710888905	25.775070204445843	24.103876335196702
38-39	24.52087106002152	25.56891845786421	25.866237566595807	24.043972915518463
40-41	24.45640763194499	25.551400152218985	25.719628375718447	24.272563840117574
42-43	24.335255754140096	25.459740702831795	25.706374826129174	24.49862871689893
44-45	24.336764821667586	25.562816576122614	25.737671574416716	24.362747027793088
46-47	24.373048053959007	25.56859039970606	25.483820171640026	24.574541374694906
48-49	24.294084455292232	25.782713959530746	25.359354906437815	24.563846678739207
50-51	24.309437577093668	25.815191717187624	25.40725139752775	24.468119308190957
52-53	24.43537910400756	25.823163530430676	25.420373723853768	24.321083641708004
54-55	24.158104348738945	25.49802508988794	25.516527570007614	24.82734299136551
56-57	24.211020129648585	25.73816366165394	25.335177020182137	24.71563918851534
58-59	24.292116106343332	25.78599454111225	25.502486680838782	24.41940267170564
60-61	24.480454294937406	25.49041414061885	25.58860194735323	24.44052961709052
62-63	24.284078681468653	25.524400965803217	25.761291761803534	24.4302285909246
64-65	24.202556229168305	25.279505550744037	25.696040994147438	24.821897225940216
66-67	24.16220507571582	25.88021284413301	25.355844684145605	24.601737396005564
68-69	24.1466551190195	25.665498779623654	25.48191743432276	24.70592866703409
70-71	24.10886281920059	25.514657638506154	25.60277405978532	24.77370548250794
72-73	24.317540613599977	25.69581135343674	25.2885927617248	24.698055271238488
74-75	23.973341994068708	25.751351599611578	25.53007637193922	24.745230034380494
76-77	24.037838227961053	25.46282444951841	25.5571083641708	24.942228958349734
78-79	24.069758286749074	25.43438180720678	25.58528855995591	24.910571346088233
80-81	24.112012177518828	25.71437944518804	25.337243786578483	24.83636459071464
82-83	24.06067107576831	25.834284701991972	25.328254993045167	24.776789229194552
84-85	24.189597931921373	25.70204445844159	25.298696952995826	24.809660656641206
86-87	23.91573498149752	25.831004120410466	25.41807731674671	24.8351835813453
88-89	23.888440542739417	25.623638558643673	25.40977744534551	25.078143453271394
90-91	24.0138309546344	25.44200168294111	25.508007092629015	25.03616026979547
92-93	23.929713895979532	25.762221776790245	25.485241092608916	24.822823234621303
94-95	23.891277518356507	25.545610283711444	25.432102086516867	25.131010111415186
96-97	23.734051389856663	25.628627517364187	25.41030395364126	25.227017139137885
98-99	23.794714326955884	25.594146130225965	25.47319108731596	25.13794845550219
100-101	23.779394010970265	25.542640999396372	25.51692123979739	25.161043749835972
102-103	23.667296538330316	25.5590767131197	25.51892239456211	25.254704353987872
104-105	23.666706033645646	25.575381203579774	25.489528383591843	25.26838437918274
106-107	23.615363114929423	25.436908428350037	25.57003460361993	25.377693853100613
108-109	23.510009923808116	25.481485126590066	25.646171132380317	25.3623338172215
110-111	23.438946536186574	25.676277708703083	25.506835114148213	25.377940640962134
112-113	23.50256871223206	25.76930142837459	25.45839867201186	25.26973118738149
114-115	23.52844302635559	25.71233048381456	25.427148965597784	25.332077524232066
116-117	23.207011565106335	25.52997305641553	25.69777585024183	25.565239528236305
118-119	23.424504245249132	25.474447310587685	25.58943203451444	25.511616409648745
120-121	23.1481420729891	25.601823480859093	25.686396985010347	25.563637461141457
122-123	23.21216853280143	25.721001643598335	25.490438588145754	25.576391235454484
124-125	23.05951831845151	25.628051352945537	25.44545406231403	25.866976266288916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	23.0
2	35.5
3	46.0
4	43.5
5	60.5
6	129.5
7	318.5
8	480.5
9	536.0
10	505.5
11	439.0
12	456.5
13	508.0
14	552.5
15	626.0
16	696.0
17	714.0
18	792.5
19	876.0
20	928.5
21	1004.0
22	1108.0
23	1334.0
24	1555.0
25	1768.5
26	2223.5
27	2890.0
28	3639.0
29	4491.0
30	5631.0
31	7065.0
32	8903.0
33	11064.0
34	13281.5
35	15647.5
36	18908.0
37	22363.0
38	25087.5
39	28299.0
40	32066.0
41	35118.0
42	38447.0
43	43755.5
44	49035.5
45	54882.5
46	58984.0
47	61653.5
48	65853.5
49	68473.0
50	69906.5
51	70996.5
52	71140.5
53	70360.5
54	70211.5
55	69310.0
56	64859.0
57	58926.5
58	54362.0
59	49801.0
60	42300.0
61	33836.0
62	27019.0
63	20661.5
64	14857.0
65	10746.5
66	7908.5
67	5809.5
68	4363.5
69	3250.5
70	2365.0
71	1743.0
72	1240.5
73	893.5
74	704.5
75	519.5
76	380.0
77	280.5
78	218.5
79	182.0
80	152.0
81	139.0
82	128.0
83	117.5
84	109.0
85	103.5
86	103.0
87	87.5
88	86.0
89	89.5
90	79.0
91	72.0
92	69.0
93	68.0
94	69.5
95	62.5
96	45.0
97	37.5
98	29.0
99	10.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3497099965881952
2	0.0
3	0.0
4	0.01594362648610346
5	0.17623284255832875
6	0.04691231661548959
7	0.0238170222817101
8	0.008726347006797365
9	0.006823609689525759
10-11	0.0022964071070519384
12-13	0.058984856835419784
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.6402907907513844E-4
92-93	4.2647560559535994E-4
94-95	6.561163163005537E-5
96-97	3.936697897803323E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.3122326326011073E-4
108-109	4.920872372254153E-4
110-111	3.280581581502769E-4
112-113	6.561163163005538E-4
114-115	1.9683489489016615E-4
116-117	6.23310500485526E-4
118-119	2.952523423352492E-4
120-121	1.3122326326011073E-4
122-123	0.0016402907907513845
124-125	6.561163163005537E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1524120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.69228285792043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.94612588501332	49.93751294351076
2	10.383953533415982	12.81219597134002
3	3.4238309315376894	6.336718388583609
4	1.6322316664507577	4.027843906253399
5	0.9320758938555893	2.875094484439402
6	0.5693055231155246	2.1073054418771586
7	0.4015037856449655	1.7338779578774544
8	0.29503154854675934	1.456093579596557
9	0.2153985745447124	1.195958680920473
>10	1.1229119846144953	12.102482412204193
>50	0.05364943743611832	2.2529631177279685
>100	0.02249576195259747	2.3539300918157307
>500	0.001061052765306665	0.4704521607610195
>1k	4.24421106122666E-4	0.3375708630923302
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1689	0.11081804582316351	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.561163163005538E-5
2	0.0	0.0	0.0	0.0	1.3122326326011076E-4
3	0.0	0.0	0.0	0.0	1.3122326326011076E-4
4	0.0	0.0	0.0	0.0	1.9683489489016613E-4
5	0.0	0.0	0.0	0.0	1.9683489489016613E-4
6	6.561163163005538E-5	0.0	0.0	0.0	3.280581581502769E-4
7	6.561163163005538E-5	0.0	0.0	0.0	3.280581581502769E-4
8	6.561163163005538E-5	0.0	0.0	0.0	3.280581581502769E-4
9	6.561163163005538E-5	0.0	0.0	0.0	3.280581581502769E-4
10-11	6.561163163005538E-5	0.0	0.0	0.0	3.9366978978033225E-4
12-13	6.561163163005538E-5	0.0	0.0	6.561163163005538E-5	3.9366978978033225E-4
14-15	6.561163163005538E-5	0.0	0.0	6.561163163005538E-5	7.545337637456368E-4
16-17	6.561163163005538E-5	0.0	0.0	1.3122326326011076E-4	7.873395795606645E-4
18-19	6.561163163005538E-5	0.0	0.0	1.9683489489016613E-4	8.529512111907199E-4
20-21	6.561163163005538E-5	0.0	0.0	2.624465265202215E-4	8.529512111907199E-4
22-23	6.561163163005538E-5	0.0	0.0	3.9366978978033225E-4	8.857570270057475E-4
24-25	6.561163163005538E-5	0.0	0.0	5.576988688554708E-4	9.185628428207752E-4
26-27	6.561163163005538E-5	0.0	0.0	0.0011153977377109413	9.185628428207752E-4
28-29	9.841744744508308E-5	0.0	0.0	0.0033461932131328247	9.51368658635803E-4
30-31	1.3122326326011076E-4	0.0	0.0	0.01289268561530588	9.841744744508306E-4
32-33	1.3122326326011076E-4	0.0	0.0	0.026867963152507675	9.841744744508306E-4
34-35	1.3122326326011076E-4	0.0	0.0	0.04228669658557069	9.841744744508306E-4
36-37	1.3122326326011076E-4	0.0	0.0	0.06616933049891084	9.841744744508306E-4
38-39	1.3122326326011076E-4	0.0	0.0	0.10740624097840065	9.841744744508306E-4
40-41	1.3122326326011076E-4	0.0	0.0	0.1545153924887804	9.841744744508306E-4
42-43	1.3122326326011076E-4	0.0	0.0	0.1996233892344435	0.0010169802902658585
44-45	1.3122326326011076E-4	0.0	0.0	0.2489633362202451	0.0010825919218959137
46-47	1.3122326326011076E-4	0.0	0.0	0.30250242763037033	0.001148203553525969
48-49	1.3122326326011076E-4	0.0	0.0	0.35850195522662254	0.0011810093693409968
50-51	1.3122326326011076E-4	0.0	0.0	0.4208330052751752	0.0011810093693409968
52-53	1.3122326326011076E-4	0.0	0.0	0.4804411726110805	0.0013122326326011075
54-55	1.3122326326011076E-4	0.0	0.0	0.5435923680550088	0.0014434558958612183
56-57	1.3122326326011076E-4	0.0	0.0	0.619636249114243	0.0014434558958612183
58-59	1.3122326326011076E-4	0.0	0.0	0.696565887200483	0.0014434558958612183
60-61	1.3122326326011076E-4	0.0	0.0	0.7822546781093351	0.0014434558958612183
62-63	1.3122326326011076E-4	0.0	0.0	0.8679434690181875	0.001574679159121329
64-65	1.3122326326011076E-4	0.0	0.0	0.9550757158229011	0.001574679159121329
66-67	1.3122326326011076E-4	0.0	0.0	1.0634989370915675	0.001574679159121329
68-69	1.3122326326011076E-4	0.0	0.0	1.1780568459176441	0.001574679159121329
70-71	1.3122326326011076E-4	0.0	0.0	1.3066228380967377	0.001574679159121329
72-73	1.3122326326011076E-4	0.0	0.0	1.4272170170327796	0.001968348948901661
74-75	1.3122326326011076E-4	0.0	0.0	1.5550940870797576	0.001968348948901661
76-77	1.3122326326011076E-4	0.0	0.0	1.6973729102695327	0.001968348948901661
78-79	1.3122326326011076E-4	0.0	0.0	1.862812639424717	0.001968348948901661
80-81	1.3122326326011076E-4	0.0	0.0	2.0345182794005723	0.002001154764716689
82-83	1.3122326326011076E-4	0.0	0.0	2.2131787523292132	0.0022964071070519384
84-85	1.3122326326011076E-4	0.0	0.0	2.404207017820119	0.0023292129228669658
86-87	1.3122326326011076E-4	0.0	0.0	2.6026165918694066	0.0023620187386819936
88-89	1.3122326326011076E-4	0.0	0.0	2.8283862163084272	0.0023620187386819936
90-91	1.3122326326011076E-4	0.0	0.0	3.0726583208671236	0.0023620187386819936
92-93	1.3122326326011076E-4	0.0	0.0	3.3223433850352992	0.0023948245544970215
94-95	1.3122326326011076E-4	0.0	0.0	3.5823294753693933	0.00255885363357216
96-97	1.3122326326011076E-4	0.0	0.0	3.8517964464740313	0.00255885363357216
98-99	1.3122326326011076E-4	0.0	0.0	4.155545495105372	0.00255885363357216
100-101	1.3122326326011076E-4	0.0	0.0	4.491345825787995	0.00255885363357216
102-103	1.3122326326011076E-4	0.0	0.0	4.848601160013647	0.0027884943442773535
104-105	1.3122326326011076E-4	0.0	0.0	5.212811327192084	0.0028213001600923813
106-107	1.3122326326011076E-4	0.0	0.0	5.596442537333019	0.0028541059759074087
108-109	1.3122326326011076E-4	0.0	0.0	5.9978544996456975	0.0029525234233524923
110-111	1.3122326326011076E-4	0.0	0.0	6.437550849014514	0.0031165525024276306
112-113	1.3122326326011076E-4	0.0	0.0	6.891878592236832	0.0032149699498727133
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1620	0.0	63.456257	1
GTATCAA	2695	0.0	48.567574	1
GTACATG	6635	0.0	36.84202	1
TACATGG	6995	0.0	35.02101	2
ACATGGG	6950	0.0	34.818962	3
TCAACGC	3570	0.0	34.059364	4
TATCAAC	3990	0.0	32.714916	2
CAACGCA	3780	0.0	32.009495	5
ATCAACG	4010	0.0	31.214005	3
CATGGGG	6700	0.0	30.958435	4
AACGCAG	3925	0.0	30.520254	6
GTGGTAT	960	0.0	28.638325	1
ATGGGGG	3800	0.0	28.23342	5
TGGTATC	1050	0.0	24.976866	2
ACGCAGA	5095	0.0	23.273142	7
GTATAGG	395	0.0	22.696306	1
CGCAGAG	5315	0.0	22.305416	8
AGAGTAC	5220	0.0	22.151636	5
CAGAGTA	5870	0.0	20.714127	4
AGTACAT	4520	0.0	20.565168	7
>>END_MODULE