FastQCFastQC Report
Mon 15 Aug 2016
SRR2921311_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921311_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070130
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11200.060.5739441
GTATCAA15850.042.803042
GTACATG50800.040.18181
TACATGG52600.038.0147862
TCAACGC18300.037.7194445
CAACGCA18900.036.8316846
ACATGGG53500.036.707873
AACGCAG20750.033.5431947
CATGGGG49600.033.4735074
ATCAACG21100.032.715554
TATCAAC21550.032.3100473
ATGGGGG28700.031.5152035
GTATAGA4000.026.7800561
GTATAGG2455.529728E-1026.7193341
ACGCAGA26800.025.9709428
TGGGGGG18600.024.3107416
CGCAGAG28500.024.222122
CTAGTAC2001.8226547E-623.8044953
GAGTACA35450.022.9987031
AGTACAT35500.022.6310352
Produced by FastQC (version 0.11.5)