##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921311_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1070130
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.003580873351837	31.0	31.0	34.0	26.0	34.0
2	30.004860157177166	31.0	31.0	34.0	25.0	34.0
3	30.17531608309271	31.0	31.0	34.0	26.0	34.0
4	33.63659088148169	37.0	35.0	37.0	28.0	37.0
5	33.612831151355444	37.0	35.0	37.0	28.0	37.0
6	33.70142225710895	37.0	35.0	37.0	29.0	37.0
7	33.54974162017699	37.0	35.0	37.0	28.0	37.0
8	33.51979105342342	37.0	35.0	37.0	28.0	37.0
9	35.06515563529665	39.0	37.0	39.0	28.0	39.0
10-11	35.1274686253072	39.0	37.0	39.0	28.0	39.0
12-13	35.09257052881426	39.0	37.0	39.0	27.0	39.0
14-15	36.34731995178156	40.0	38.0	41.0	27.0	41.0
16-17	36.31539859643221	40.0	38.0	41.0	27.0	41.0
18-19	36.28199424369002	40.0	37.5	41.0	27.0	41.0
20-21	36.32223468176764	40.0	38.0	41.0	27.0	41.0
22-23	36.25971424032595	40.0	37.5	41.0	27.0	41.0
24-25	36.24725594086699	40.0	37.0	41.0	27.0	41.0
26-27	36.21531869959725	40.0	37.0	41.0	27.0	41.0
28-29	36.150804575145074	40.0	37.0	41.0	27.0	41.0
30-31	36.073613953444905	40.0	37.0	41.0	27.0	41.0
32-33	36.001617560483304	40.0	37.0	41.0	27.0	41.0
34-35	35.88390289030305	40.0	37.0	41.0	26.5	41.0
36-37	35.76918598674927	40.0	36.5	41.0	25.5	41.0
38-39	35.65741171633353	40.0	36.0	41.0	25.0	41.0
40-41	35.54826142618187	40.0	36.0	41.0	25.0	41.0
42-43	35.329581919953654	40.0	35.5	41.0	24.0	41.0
44-45	35.04623363516582	40.0	35.0	41.0	23.5	41.0
46-47	34.806281947053165	39.0	35.0	41.0	23.0	41.0
48-49	34.58850840552083	39.0	35.0	41.0	22.0	41.0
50-51	34.40824432545578	39.0	34.5	41.0	21.0	41.0
52-53	34.204786334370596	39.0	34.0	40.0	20.0	41.0
54-55	33.98601898834721	38.0	34.0	40.0	18.5	41.0
56-57	33.740117088578025	38.0	34.0	40.0	18.0	41.0
58-59	33.462675562782096	38.0	33.0	40.0	15.5	41.0
60-61	33.1672857503294	37.0	33.0	40.0	13.0	41.0
62-63	32.8474040537131	36.5	33.0	39.5	12.0	41.0
64-65	32.49852728173212	36.0	32.5	39.0	8.0	41.0
66-67	32.15678702587536	35.5	32.0	39.0	7.5	40.0
68-69	31.80985674637661	35.0	32.0	38.5	7.0	40.0
70-71	31.780960257165017	35.0	32.0	38.0	6.0	40.0
72-73	31.50023969050489	35.0	32.0	37.0	2.0	39.5
74-75	31.183328661003806	35.0	32.0	37.0	2.0	39.0
76-77	30.88908170035416	35.0	32.0	36.0	2.0	39.0
78-79	30.613098408604564	35.0	31.5	36.0	2.0	37.5
80-81	30.337928569426143	35.0	31.0	35.0	2.0	37.0
82-83	30.121387588423836	35.0	31.0	35.0	2.0	36.5
84-85	29.90620812424659	35.0	31.0	35.0	2.0	36.0
86-87	29.73880743461075	35.0	31.0	35.0	2.0	36.0
88-89	29.60047050358368	35.0	31.0	35.0	2.0	35.5
90-91	29.482462878341885	34.0	31.0	35.0	2.0	35.0
92-93	29.37312943287264	34.0	31.0	35.0	2.0	35.0
94-95	29.251734835954508	34.0	30.0	35.0	2.0	35.0
96-97	29.15580910730472	34.0	30.0	35.0	2.0	35.0
98-99	29.071001186771703	34.0	30.0	35.0	2.0	35.0
100-101	28.981892386906264	34.0	30.0	35.0	2.0	35.0
102-103	28.11849494921178	33.5	28.0	34.5	2.0	35.0
104-105	28.744516554063523	34.0	29.5	35.0	2.0	35.0
106-107	28.83860886060572	34.0	30.0	35.0	2.0	35.0
108-109	28.781284984067355	34.0	30.0	35.0	2.0	35.0
110-111	28.698136674983413	34.0	30.0	35.0	2.0	35.0
112-113	28.610648706231952	34.0	30.0	35.0	2.0	35.0
114-115	28.480992963471728	34.0	29.0	35.0	2.0	35.0
116-117	28.351039593320436	34.0	29.0	35.0	2.0	35.0
118-119	28.35977311167802	34.0	29.5	35.0	2.0	35.0
120-121	28.537534692046762	34.0	30.0	35.0	2.0	35.0
122-123	28.408800799902814	34.0	30.0	35.0	2.0	35.0
124-125	27.805273191107624	34.0	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48052.0
3	6466.0
4	4051.0
5	3443.0
6	3470.0
7	3777.0
8	4123.0
9	4294.0
10	4629.0
11	5028.0
12	4992.0
13	4802.0
14	4834.0
15	4386.0
16	3893.0
17	3692.0
18	3381.0
19	3241.0
20	3318.0
21	3373.0
22	3814.0
23	4792.0
24	6534.0
25	8376.0
26	9160.0
27	10538.0
28	12534.0
29	15125.0
30	18418.0
31	23126.0
32	30106.0
33	41227.0
34	63625.0
35	104761.0
36	212638.0
37	308386.0
38	69699.0
39	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22523313999241	17.476854231661427	11.573725323797756	25.7241873045484
2	16.662850477989313	19.02078779218376	38.06204281670239	26.254318913124543
3	20.5790461878073	22.342315567929376	28.047824254369857	29.030813989893467
4	13.533655396330948	13.98516042868211	34.16811763243509	38.31306654255185
5	14.341266384932124	37.920709357985416	32.81733919729953	14.920685059782922
6	35.696084205016035	33.46251998093048	15.961748787122465	14.879647026931023
7	31.25921744477945	31.30660141946313	18.620032860365875	18.81414827539155
8	28.245483356339847	35.22397935675854	18.156669193177112	18.373868093724504
9	27.589616395008377	15.78913921346351	17.53366484958875	39.08757954193936
10-11	27.13960159489141	25.360651899949676	25.88063173250757	21.61911477265134
12-13	26.343542635952318	22.46326935989649	26.641658571277045	24.55152943287415
14-15	23.724208246071953	23.960269554726317	23.316614660285722	28.998907538916008
16-17	24.451862387601654	27.351323922181376	24.083711654664427	24.113102035552547
18-19	24.480299047952805	26.62765025407394	25.549722562934406	23.34232813503884
20-21	24.990584719303147	25.430930154971314	25.502654501719046	24.07583062400649
22-23	24.940622904629848	25.00973069455899	25.50983933256547	24.539807068245693
24-25	24.489295281457068	25.35699917434961	25.514185813859058	24.639519730334264
26-27	24.29322777073778	25.659439142640334	25.547344300778636	24.49998878584325
28-29	24.38446352432685	25.930120904152794	25.476222183283685	24.20919338823667
30-31	24.841155103082137	25.595031722253424	25.444060149392232	24.119753025272203
32-33	24.451390151166468	25.565454926916658	25.47756146818056	24.505593453736314
34-35	24.42662377744456	25.721851494822406	25.709141731153924	24.142382996579112
36-37	24.53237151226551	25.72899392413557	25.573067141035473	24.165567422563452
38-39	24.5067520209336	25.918695388065977	25.469884584832485	24.104668006167937
40-41	24.578138280648027	25.649398134757444	25.56010311682134	24.212360467773188
42-43	24.299882482401564	25.616873938428252	25.523607486548556	24.559636092621624
44-45	24.19667305266109	25.663380215877762	25.733470398579506	24.406476332881642
46-47	24.27281006591893	25.6383837222723	25.592216641363557	24.496589570445217
48-49	24.086609967772187	25.861779739700957	25.547258346311843	24.50435194621502
50-51	24.17836843708706	25.821070849835433	25.570712460726718	24.429848252350787
52-53	24.386255104806228	25.77887427925276	25.551739605820178	24.283131010120833
54-55	24.092540475477346	25.545634526613657	25.742959633628537	24.618865364280467
56-57	24.210562221654232	25.756004745574856	25.406114390035228	24.627318642735688
58-59	24.227526941409938	25.900293770358214	25.60470107335755	24.267478214874302
60-61	24.558149097374244	25.68288597267942	25.573127054475282	24.185837875471055
62-63	24.39451192986604	25.778584788889287	25.553505319529208	24.273397961715464
64-65	24.239548523112116	25.596165922802612	25.57130815409574	24.592977399989532
66-67	24.262313618865033	25.856204948109283	25.448059991467968	24.433421441557705
68-69	24.119453170594717	25.739783628854283	25.59077322335814	24.54998997719286
70-71	24.04692383567686	25.560743667847426	25.825818669634685	24.566513826841028
72-73	24.108057341507177	25.854795903110052	25.492346366626794	24.54480038875598
74-75	23.96602888273881	25.939188971348553	25.63084912373824	24.4639330221744
76-77	24.03605157754855	25.537437457830176	25.73620801474285	24.690302949878422
78-79	23.937825964659147	25.490728375944894	25.833408871939167	24.738036787456792
80-81	24.11887372458575	25.799988505677305	25.49716450011144	24.583973269625506
82-83	24.18036942425756	25.80348677960938	25.571445993511645	24.444697802621416
84-85	24.081893184103595	25.924788884029205	25.440175397279326	24.55314253458788
86-87	23.911527912116668	25.939227422889477	25.616627182714907	24.532617482278948
88-89	23.893256179934884	25.664438787525373	25.691866745913373	24.75043828662637
90-91	24.10896265955687	25.576666264824922	25.674229702085743	24.640141373532458
92-93	24.096123194991605	25.788203937503475	25.682834389443045	24.43283847806187
94-95	24.10783916550201	25.553931030744636	25.704810914190045	24.63341888956331
96-97	23.848530117486867	25.656415751080925	25.76580474741702	24.72924938401519
98-99	23.859837176669807	25.57615002212502	25.709087052026742	24.85492574917843
100-101	23.787444155307703	25.559802008049203	25.91100268353576	24.741751153107327
102-103	23.574007506982017	25.641861914705622	25.875678284748528	24.908452293563833
104-105	23.70657370117688	25.630364988605937	25.879553246059032	24.78350806415815
106-107	23.669597365361682	25.599083035216978	25.90752598939117	24.82379361003017
108-109	23.58891928704766	25.61595989000591	25.995425479471894	24.799695343474532
110-111	23.518895449354513	25.902204204305235	25.772396700702586	24.806503645637665
112-113	23.495810318862468	25.946398808675514	25.89976632358926	24.65802454887276
114-115	23.667892565130625	25.892872578671422	25.791246514926886	24.64798834127107
116-117	23.4233080848316	25.768657312681615	25.953220106784762	24.854814495702023
118-119	23.48074998163278	25.661840025943413	25.981265901685525	24.876144090738286
120-121	23.246204090558837	25.834473909124338	26.11087564912353	24.808446351193293
122-123	23.32639165016907	25.939311591240305	25.877448531943813	24.856848226646814
124-125	23.270936292033667	25.78100652316422	25.946083087758776	25.001974097043338
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	216.0
1	190.0
2	158.5
3	134.5
4	108.0
5	99.0
6	95.0
7	107.0
8	141.0
9	163.0
10	161.0
11	161.0
12	185.0
13	212.0
14	235.0
15	268.5
16	271.5
17	284.0
18	347.0
19	399.5
20	428.0
21	478.0
22	573.5
23	765.5
24	961.0
25	1194.0
26	1574.5
27	2052.5
28	2650.0
29	3495.5
30	4495.5
31	5528.5
32	6964.0
33	8609.0
34	10352.0
35	12274.5
36	14680.0
37	17037.5
38	19250.5
39	21771.5
40	24069.0
41	26121.0
42	28433.5
43	31722.0
44	35048.0
45	38482.0
46	40899.0
47	42903.0
48	46327.5
49	48751.5
50	49306.0
51	49408.5
52	49043.0
53	48432.0
54	48322.0
55	46988.0
56	43867.5
57	40054.0
58	36853.5
59	33553.5
60	28490.0
61	23261.0
62	18726.5
63	14158.5
64	10098.0
65	7298.0
66	5481.0
67	4111.5
68	3035.0
69	2233.5
70	1670.0
71	1220.5
72	836.5
73	584.5
74	438.0
75	287.5
76	183.0
77	125.5
78	83.5
79	48.5
80	42.5
81	39.0
82	25.0
83	18.0
84	18.0
85	13.5
86	9.0
87	7.5
88	5.0
89	4.5
90	6.5
91	4.5
92	3.5
93	4.0
94	2.5
95	1.0
96	0.5
97	2.0
98	1.5
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01607281358339641
2	0.011867717006345023
3	0.012054610187547308
4	0.014297328361974714
5	0.008597086335305057
6	0.03364077261641109
7	0.013830095408969003
8	0.013362862455963293
9	0.009064319288310765
10-11	0.00827002326820106
12-13	0.007148664180987357
14-15	0.006447814751478792
16-17	0.00485922271125938
18-19	0.00630764486557708
20-21	0.005700242026669656
22-23	0.00584041191257137
24-25	0.006027305093773654
26-27	0.005046115892461663
28-29	0.00630764486557708
30-31	0.005700242026669656
32-33	0.00789623690579649
34-35	0.008083130086998777
36-37	0.007148664180987357
38-39	0.0074757272480913545
40-41	0.007615897133993066
42-43	0.007242110771588499
44-45	0.0074757272480913545
46-47	0.009671722127218188
48-49	0.008550363040004486
50-51	0.005980581798473082
52-53	0.005606795436068515
54-55	0.006401091456178221
56-57	0.007522450543391924
58-59	0.007148664180987357
60-61	0.005373178959565661
62-63	0.005933858503172512
64-65	0.003924756805247961
66-67	0.004158373281750815
68-69	0.006587984637380505
70-71	0.005186285778363376
72-73	0.004672329530057096
74-75	0.004158373281750815
76-77	0.00485922271125938
78-79	0.003457523852242251
80-81	0.00341080055694168
82-83	0.005560072140767944
84-85	0.009531552241316476
86-87	0.007008494295085644
88-89	0.005046115892461663
90-91	0.00532645566426509
92-93	0.008083130086998777
94-95	0.00686832440918393
96-97	0.008924149402409053
98-99	0.006961770999785073
100-101	0.008083130086998777
102-103	0.005560072140767944
104-105	0.005186285778363376
106-107	0.007288834066889069
108-109	0.006774877818582789
110-111	0.007849513610495922
112-113	0.005653518731369086
114-115	0.002850121013334828
116-117	0.003130460785138254
118-119	0.15423359778718473
120-121	0.012101333482847879
122-123	0.0022894414697279767
124-125	0.0019156551073234093
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1070130.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.81873297668753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.27758676714164	53.33128926625593
2	9.74534775867279	12.633621882687438
3	3.132681491006718	6.091693351997274
4	1.4775653785567446	3.830956629130714
5	0.8651721896329994	2.803968256933873
6	0.5315997441626782	2.0674573120413626
7	0.37956124517365897	1.7221875299447302
8	0.2864034245125786	1.4851445677671773
9	0.20775484285385143	1.2119765115201728
>10	1.0414614505469593	11.54323371006316
>50	0.042745639463576714	1.8909393081907016
>100	0.012120068275832032	1.3875316734674896
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.344659060114191E-5
7	0.0	0.0	0.0	0.0	9.344659060114191E-5
8	0.0	0.0	0.0	0.0	9.344659060114191E-5
9	0.0	0.0	0.0	9.344659060114191E-5	9.344659060114191E-5
10-11	0.0	0.0	0.0	9.344659060114191E-5	9.344659060114191E-5
12-13	0.0	0.0	0.0	9.344659060114191E-5	1.8689318120228382E-4
14-15	0.0	0.0	0.0	9.344659060114191E-5	2.8033977180342575E-4
16-17	0.0	0.0	0.0	9.344659060114191E-5	4.672329530057096E-4
18-19	0.0	0.0	0.0	9.344659060114191E-5	5.606795436068515E-4
20-21	0.0	0.0	0.0	1.8689318120228382E-4	5.606795436068515E-4
22-23	0.0	0.0	0.0	3.7378636240456765E-4	6.074028389074224E-4
24-25	0.0	0.0	0.0	4.6723295300570955E-4	6.541261342079935E-4
26-27	0.0	0.0	4.6723295300570956E-5	7.942960201097063E-4	6.541261342079935E-4
28-29	0.0	0.0	9.344659060114191E-5	0.0027099511274331156	6.541261342079935E-4
30-31	0.0	0.0	9.344659060114191E-5	0.011680823825142739	6.541261342079935E-4
32-33	0.0	0.0	9.344659060114191E-5	0.02714623456963173	6.541261342079935E-4
34-35	0.0	0.0	9.344659060114191E-5	0.048078270864287516	6.541261342079935E-4
36-37	0.0	0.0	9.344659060114191E-5	0.0775606701989478	6.541261342079935E-4
38-39	0.0	0.0	9.344659060114191E-5	0.12727425639875528	6.541261342079935E-4
40-41	0.0	0.0	9.344659060114191E-5	0.18525786586676385	6.541261342079935E-4
42-43	0.0	0.0	9.344659060114191E-5	0.24141926681805015	6.541261342079935E-4
44-45	0.0	0.0	9.344659060114191E-5	0.3011783615074804	6.541261342079935E-4
46-47	0.0	0.0	9.344659060114191E-5	0.3623391550559278	7.008494295085644E-4
48-49	0.0	0.0	9.344659060114191E-5	0.4265369627989123	9.344659060114192E-4
50-51	0.0	0.0	9.344659060114191E-5	0.5008737256221207	9.811892013119902E-4
52-53	0.0	0.0	9.344659060114191E-5	0.5778269929821611	0.0010279124966125612
54-55	0.0	0.0	9.344659060114191E-5	0.6597796529393625	0.0010279124966125612
56-57	0.0	0.0	9.344659060114191E-5	0.7448627736817022	0.0010279124966125612
58-59	0.0	0.0	9.344659060114191E-5	0.838122471101642	0.0010279124966125612
60-61	0.0	0.0	9.344659060114191E-5	0.9364282844140431	0.0010279124966125612
62-63	0.0	0.0	9.344659060114191E-5	1.0549652845915918	0.0010279124966125612
64-65	0.0	0.0	9.344659060114191E-5	1.1648117518432342	0.0010279124966125612
66-67	0.0	0.0	9.344659060114191E-5	1.282974965658378	0.0010279124966125612
68-69	0.0	0.0	9.344659060114191E-5	1.415388784540196	0.0010279124966125612
70-71	0.0	0.0	9.344659060114191E-5	1.5608384028108735	0.0012148056778148448
72-73	0.0	0.0	9.344659060114191E-5	1.705166661994337	0.001308252268415987
74-75	0.0	0.0	9.344659060114191E-5	1.849354751291899	0.001308252268415987
76-77	0.0	0.0	9.344659060114191E-5	2.0165774251726427	0.001308252268415987
78-79	0.0	0.0	9.344659060114191E-5	2.2044985188715387	0.001308252268415987
80-81	0.0	0.0	9.344659060114191E-5	2.394195097791857	0.001308252268415987
82-83	0.0	4.6723295300570956E-5	9.344659060114191E-5	2.594544588040705	0.0014951454496182706
84-85	0.0	9.344659060114191E-5	9.344659060114191E-5	2.799753301000813	0.0014951454496182706
86-87	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	3.0256604337790733	0.0014951454496182706
88-89	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	3.278713801126966	0.0014951454496182706
90-91	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	3.554661583172138	0.0014951454496182706
92-93	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	3.8368702867875863	0.0014951454496182706
94-95	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	4.121274985282162	0.0017754852214216963
96-97	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	4.42801341893041	0.0017754852214216963
98-99	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	4.76923364451048	0.0017754852214216963
100-101	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	5.137740274546083	0.0017754852214216963
102-103	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	5.527739620419949	0.0017754852214216963
104-105	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	5.928999280461252	0.0017754852214216963
106-107	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	6.338248623998954	0.0018222085167222675
108-109	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	6.768430003831311	0.0019156551073234093
110-111	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	7.242764897722706	0.0019623784026239805
112-113	9.344659060114191E-5	9.344659060114191E-5	9.344659060114191E-5	7.725977217721212	0.0019623784026239805
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1120	0.0	60.573944	1
GTATCAA	1585	0.0	42.80304	2
GTACATG	5080	0.0	40.1818	1
TACATGG	5260	0.0	38.014786	2
TCAACGC	1830	0.0	37.719444	5
CAACGCA	1890	0.0	36.831684	6
ACATGGG	5350	0.0	36.70787	3
AACGCAG	2075	0.0	33.543194	7
CATGGGG	4960	0.0	33.473507	4
ATCAACG	2110	0.0	32.71555	4
TATCAAC	2155	0.0	32.310047	3
ATGGGGG	2870	0.0	31.515203	5
GTATAGA	400	0.0	26.780056	1
GTATAGG	245	5.529728E-10	26.719334	1
ACGCAGA	2680	0.0	25.970942	8
TGGGGGG	1860	0.0	24.310741	6
CGCAGAG	2850	0.0	24.22212	2
CTAGTAC	200	1.8226547E-6	23.804495	3
GAGTACA	3545	0.0	22.998703	1
AGTACAT	3550	0.0	22.631035	2
>>END_MODULE